Luteinizing Hormone (LH) Drives Diverse Intracellular Calcium Second Messenger Signals in Isolated Porcine Ovarian Thecal Cells: Preferential Recruitment of Intracellular Ca2+ Oscillatory Cells by Higher Concentrations of LH

Abstract The present study examines Ca2+ second messenger signaling driven by LH in isolated porcine thecal cells. To this end, we implemented semiquantitative fluorescent (fura-2) videomicroscopic imaging of single thecal cells in vitro. Stimulation of 388 cells with LH (5 μg/ml) elicited an intrac...

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Veröffentlicht in:	Endocrinology (Philadelphia) 2000-06, Vol.141 (6), p.2220-2228
Hauptverfasser:	Aguirre, C., Jayes, F. C. L., Veldhuis, J. D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplitudes Animals Calcium (extracellular) Calcium (intracellular) Calcium - metabolism Calcium chloride Calcium ions Calcium signalling Cells, Cultured Chelating Agents - pharmacology Cobalt - pharmacology Egtazic Acid - pharmacology Estrenes - pharmacology Female Fluorescence Fura-2 Granulosa cells Intracellular Intracellular signalling Luteinizing hormone Luteinizing Hormone - pharmacology Oscillations Periodicity Pertussis Pertussis Toxin Phosphodiesterase Inhibitors - pharmacology Phospholipase Phospholipase C Pyrrolidinones - pharmacology Second Messenger Systems Signal Transduction Swine Theca Cells - drug effects Theca Cells - metabolism Toxins Type C Phospholipases - antagonists & inhibitors Virulence Factors, Bordetella - pharmacology Whooping cough
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