Modulation of the levels of NMDA receptor subunit mRNA and the bindings of [3H]MK-801 in rat brain by chronic infusion of subtoxic dose of MK-801
The effects of continuous infusion of NMDA receptor antagonist MK-801 on the modulation of NMDA receptor subunits NR1, NR2A, NR2B, and NR2C were investigated by using in situ hybridization study. Differential assembly of NMDA receptor subunits determines their functional characteristics. Continuous...
Gespeichert in:
| Veröffentlicht in: | Neurochemical research 2001-05, Vol.26 (5), p.559-565 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 565 |
|---|---|
| container_issue | 5 |
| container_start_page | 559 |
| container_title | Neurochemical research |
| container_volume | 26 |
| creator | OH, Seikwan YOUNG HWA KIM HOO JAE HANN HEE LAI LEE HONG SERCK CHOI HACK SEANG KIM ING KANG HO |
| description | The effects of continuous infusion of NMDA receptor antagonist MK-801 on the modulation of NMDA receptor subunits NR1, NR2A, NR2B, and NR2C were investigated by using in situ hybridization study. Differential assembly of NMDA receptor subunits determines their functional characteristics. Continuous intracerebroventricular (i.c.v.) infusion with MK-801 (1 pmol/10 microl/h) for 7 days resulted in significant modulations in the NR1, NR2A, and NR2B mRNA levels without producing stereotypic motor syndromes. The levels of NR1 mRNA were significantly increased (9-20%) in the cerebral cortex, striatum, septum, and CA1 of hippocampus in MK-801-infused rats. The levels of NR2A mRNA were significantly decreased (11-16%) in the CA3 and dentate gyrus of hippocampus in MK-801-infused rats. In contrast to NR2A, NR2B subunit mRNA levels were increased (10-14%) in the cerebral cortex, caudate putamen, and thalamus. However, no changes of NR2C subunits in cerebellar granule layer were observed. Using quantitative ligand autoradiography, the binding of NMDA receptor ligand [3H]MK-801 was increased (12-25%) significantly in almost all brain regions except in the thalamus and cerebellum after 7 days infusion with MK-801. These results suggest that region-specific changes of NMDA receptor subunit mRNA and [3H]MK-801 binding are involved in the MK-801-infused adult rats. |
| doi_str_mv | 10.1023/A:1010977315838 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_71142458</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>390357841</sourcerecordid><originalsourceid>FETCH-LOGICAL-c309t-63698e693ded6ad003de666d09e8d7d09aa530879d0ac79f55b6d89c73977d203</originalsourceid><addsrcrecordid>eNpdkEFPGzEQha0KVELaMzdkIcRt6Xi967V7i9JCEAmVqvZUVSuv7QVHGzvYu4j8jP5jTBJUidPMPH0z8_QQOiFwSSCnXyZfCRAQVUVJySn_gEakrGjGBNADNALKiowSAUfoOMYlJBRy8hEdEVISWvByhP4tvB462VvvsG9x_2BwZ55MF1-nu8W3CQ5GmXXvA45DMzjb49XPuwmWTm_hxjpt3f0W_0Nnfxe3GQeCrcNB9rgJMnXNBquH4J1VSW-HuP-V7vX-OYnaR_Mq7HY_ocNWdtF83tcx-n31_dd0ls1_XN9MJ_NMURB9xigT3DBBtdFMaoDUMMY0CMN1lYqUJQVeCQ1SVaIty4ZpLlRFU1o6BzpGF7u76-AfBxP7emWjMl0nnfFDrCtCirxIqY7R2Ttw6Yfgkrc6zwmHgmyh0z00NCuj63WwKxk29VvSCTjfAzIq2bVBOmXjfw4YT27pC6pAigY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>221804158</pqid></control><display><type>article</type><title>Modulation of the levels of NMDA receptor subunit mRNA and the bindings of [3H]MK-801 in rat brain by chronic infusion of subtoxic dose of MK-801</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>OH, Seikwan ; YOUNG HWA KIM ; HOO JAE HANN ; HEE LAI LEE ; HONG SERCK CHOI ; HACK SEANG KIM ; ING KANG HO</creator><creatorcontrib>OH, Seikwan ; YOUNG HWA KIM ; HOO JAE HANN ; HEE LAI LEE ; HONG SERCK CHOI ; HACK SEANG KIM ; ING KANG HO</creatorcontrib><description>The effects of continuous infusion of NMDA receptor antagonist MK-801 on the modulation of NMDA receptor subunits NR1, NR2A, NR2B, and NR2C were investigated by using in situ hybridization study. Differential assembly of NMDA receptor subunits determines their functional characteristics. Continuous intracerebroventricular (i.c.v.) infusion with MK-801 (1 pmol/10 microl/h) for 7 days resulted in significant modulations in the NR1, NR2A, and NR2B mRNA levels without producing stereotypic motor syndromes. The levels of NR1 mRNA were significantly increased (9-20%) in the cerebral cortex, striatum, septum, and CA1 of hippocampus in MK-801-infused rats. The levels of NR2A mRNA were significantly decreased (11-16%) in the CA3 and dentate gyrus of hippocampus in MK-801-infused rats. In contrast to NR2A, NR2B subunit mRNA levels were increased (10-14%) in the cerebral cortex, caudate putamen, and thalamus. However, no changes of NR2C subunits in cerebellar granule layer were observed. Using quantitative ligand autoradiography, the binding of NMDA receptor ligand [3H]MK-801 was increased (12-25%) significantly in almost all brain regions except in the thalamus and cerebellum after 7 days infusion with MK-801. These results suggest that region-specific changes of NMDA receptor subunit mRNA and [3H]MK-801 binding are involved in the MK-801-infused adult rats.</description><identifier>ISSN: 0364-3190</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1023/A:1010977315838</identifier><identifier>PMID: 11513485</identifier><identifier>CODEN: NEREDZ</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Animals ; Biological and medical sciences ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Dizocilpine Maleate - administration & dosage ; Dizocilpine Maleate - metabolism ; Dizocilpine Maleate - pharmacology ; Dose-Response Relationship, Drug ; Excitatory Amino Acid Antagonists - administration & dosage ; Excitatory Amino Acid Antagonists - metabolism ; Excitatory Amino Acid Antagonists - pharmacology ; Fundamental and applied biological sciences. Psychology ; Male ; Protein Isoforms - genetics ; Protein Isoforms - metabolism ; Rats ; Rats, Sprague-Dawley ; Receptors, N-Methyl-D-Aspartate - genetics ; Receptors, N-Methyl-D-Aspartate - metabolism ; RNA, Messenger - metabolism ; Tissue Distribution ; Tritium ; Vertebrates: nervous system and sense organs</subject><ispartof>Neurochemical research, 2001-05, Vol.26 (5), p.559-565</ispartof><rights>2001 INIST-CNRS</rights><rights>Copyright Kluwer Academic Publishers May 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c309t-63698e693ded6ad003de666d09e8d7d09aa530879d0ac79f55b6d89c73977d203</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1068087$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11513485$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OH, Seikwan</creatorcontrib><creatorcontrib>YOUNG HWA KIM</creatorcontrib><creatorcontrib>HOO JAE HANN</creatorcontrib><creatorcontrib>HEE LAI LEE</creatorcontrib><creatorcontrib>HONG SERCK CHOI</creatorcontrib><creatorcontrib>HACK SEANG KIM</creatorcontrib><creatorcontrib>ING KANG HO</creatorcontrib><title>Modulation of the levels of NMDA receptor subunit mRNA and the bindings of [3H]MK-801 in rat brain by chronic infusion of subtoxic dose of MK-801</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><description>The effects of continuous infusion of NMDA receptor antagonist MK-801 on the modulation of NMDA receptor subunits NR1, NR2A, NR2B, and NR2C were investigated by using in situ hybridization study. Differential assembly of NMDA receptor subunits determines their functional characteristics. Continuous intracerebroventricular (i.c.v.) infusion with MK-801 (1 pmol/10 microl/h) for 7 days resulted in significant modulations in the NR1, NR2A, and NR2B mRNA levels without producing stereotypic motor syndromes. The levels of NR1 mRNA were significantly increased (9-20%) in the cerebral cortex, striatum, septum, and CA1 of hippocampus in MK-801-infused rats. The levels of NR2A mRNA were significantly decreased (11-16%) in the CA3 and dentate gyrus of hippocampus in MK-801-infused rats. In contrast to NR2A, NR2B subunit mRNA levels were increased (10-14%) in the cerebral cortex, caudate putamen, and thalamus. However, no changes of NR2C subunits in cerebellar granule layer were observed. Using quantitative ligand autoradiography, the binding of NMDA receptor ligand [3H]MK-801 was increased (12-25%) significantly in almost all brain regions except in the thalamus and cerebellum after 7 days infusion with MK-801. These results suggest that region-specific changes of NMDA receptor subunit mRNA and [3H]MK-801 binding are involved in the MK-801-infused adult rats.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Dizocilpine Maleate - administration & dosage</subject><subject>Dizocilpine Maleate - metabolism</subject><subject>Dizocilpine Maleate - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Excitatory Amino Acid Antagonists - administration & dosage</subject><subject>Excitatory Amino Acid Antagonists - metabolism</subject><subject>Excitatory Amino Acid Antagonists - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>Protein Isoforms - genetics</subject><subject>Protein Isoforms - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, N-Methyl-D-Aspartate - genetics</subject><subject>Receptors, N-Methyl-D-Aspartate - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Tissue Distribution</subject><subject>Tritium</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0364-3190</issn><issn>1573-6903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkEFPGzEQha0KVELaMzdkIcRt6Xi967V7i9JCEAmVqvZUVSuv7QVHGzvYu4j8jP5jTBJUidPMPH0z8_QQOiFwSSCnXyZfCRAQVUVJySn_gEakrGjGBNADNALKiowSAUfoOMYlJBRy8hEdEVISWvByhP4tvB462VvvsG9x_2BwZ55MF1-nu8W3CQ5GmXXvA45DMzjb49XPuwmWTm_hxjpt3f0W_0Nnfxe3GQeCrcNB9rgJMnXNBquH4J1VSW-HuP-V7vX-OYnaR_Mq7HY_ocNWdtF83tcx-n31_dd0ls1_XN9MJ_NMURB9xigT3DBBtdFMaoDUMMY0CMN1lYqUJQVeCQ1SVaIty4ZpLlRFU1o6BzpGF7u76-AfBxP7emWjMl0nnfFDrCtCirxIqY7R2Ttw6Yfgkrc6zwmHgmyh0z00NCuj63WwKxk29VvSCTjfAzIq2bVBOmXjfw4YT27pC6pAigY</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>OH, Seikwan</creator><creator>YOUNG HWA KIM</creator><creator>HOO JAE HANN</creator><creator>HEE LAI LEE</creator><creator>HONG SERCK CHOI</creator><creator>HACK SEANG KIM</creator><creator>ING KANG HO</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20010501</creationdate><title>Modulation of the levels of NMDA receptor subunit mRNA and the bindings of [3H]MK-801 in rat brain by chronic infusion of subtoxic dose of MK-801</title><author>OH, Seikwan ; YOUNG HWA KIM ; HOO JAE HANN ; HEE LAI LEE ; HONG SERCK CHOI ; HACK SEANG KIM ; ING KANG HO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c309t-63698e693ded6ad003de666d09e8d7d09aa530879d0ac79f55b6d89c73977d203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Dizocilpine Maleate - administration & dosage</topic><topic>Dizocilpine Maleate - metabolism</topic><topic>Dizocilpine Maleate - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Excitatory Amino Acid Antagonists - administration & dosage</topic><topic>Excitatory Amino Acid Antagonists - metabolism</topic><topic>Excitatory Amino Acid Antagonists - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>Protein Isoforms - genetics</topic><topic>Protein Isoforms - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, N-Methyl-D-Aspartate - genetics</topic><topic>Receptors, N-Methyl-D-Aspartate - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Tissue Distribution</topic><topic>Tritium</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OH, Seikwan</creatorcontrib><creatorcontrib>YOUNG HWA KIM</creatorcontrib><creatorcontrib>HOO JAE HANN</creatorcontrib><creatorcontrib>HEE LAI LEE</creatorcontrib><creatorcontrib>HONG SERCK CHOI</creatorcontrib><creatorcontrib>HACK SEANG KIM</creatorcontrib><creatorcontrib>ING KANG HO</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Neurochemical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OH, Seikwan</au><au>YOUNG HWA KIM</au><au>HOO JAE HANN</au><au>HEE LAI LEE</au><au>HONG SERCK CHOI</au><au>HACK SEANG KIM</au><au>ING KANG HO</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of the levels of NMDA receptor subunit mRNA and the bindings of [3H]MK-801 in rat brain by chronic infusion of subtoxic dose of MK-801</atitle><jtitle>Neurochemical research</jtitle><addtitle>Neurochem Res</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>26</volume><issue>5</issue><spage>559</spage><epage>565</epage><pages>559-565</pages><issn>0364-3190</issn><eissn>1573-6903</eissn><coden>NEREDZ</coden><abstract>The effects of continuous infusion of NMDA receptor antagonist MK-801 on the modulation of NMDA receptor subunits NR1, NR2A, NR2B, and NR2C were investigated by using in situ hybridization study. Differential assembly of NMDA receptor subunits determines their functional characteristics. Continuous intracerebroventricular (i.c.v.) infusion with MK-801 (1 pmol/10 microl/h) for 7 days resulted in significant modulations in the NR1, NR2A, and NR2B mRNA levels without producing stereotypic motor syndromes. The levels of NR1 mRNA were significantly increased (9-20%) in the cerebral cortex, striatum, septum, and CA1 of hippocampus in MK-801-infused rats. The levels of NR2A mRNA were significantly decreased (11-16%) in the CA3 and dentate gyrus of hippocampus in MK-801-infused rats. In contrast to NR2A, NR2B subunit mRNA levels were increased (10-14%) in the cerebral cortex, caudate putamen, and thalamus. However, no changes of NR2C subunits in cerebellar granule layer were observed. Using quantitative ligand autoradiography, the binding of NMDA receptor ligand [3H]MK-801 was increased (12-25%) significantly in almost all brain regions except in the thalamus and cerebellum after 7 days infusion with MK-801. These results suggest that region-specific changes of NMDA receptor subunit mRNA and [3H]MK-801 binding are involved in the MK-801-infused adult rats.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>11513485</pmid><doi>10.1023/A:1010977315838</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0364-3190 |
| ispartof | Neurochemical research, 2001-05, Vol.26 (5), p.559-565 |
| issn | 0364-3190 1573-6903 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71142458 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Biological and medical sciences Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Dizocilpine Maleate - administration & dosage Dizocilpine Maleate - metabolism Dizocilpine Maleate - pharmacology Dose-Response Relationship, Drug Excitatory Amino Acid Antagonists - administration & dosage Excitatory Amino Acid Antagonists - metabolism Excitatory Amino Acid Antagonists - pharmacology Fundamental and applied biological sciences. Psychology Male Protein Isoforms - genetics Protein Isoforms - metabolism Rats Rats, Sprague-Dawley Receptors, N-Methyl-D-Aspartate - genetics Receptors, N-Methyl-D-Aspartate - metabolism RNA, Messenger - metabolism Tissue Distribution Tritium Vertebrates: nervous system and sense organs |
| title | Modulation of the levels of NMDA receptor subunit mRNA and the bindings of [3H]MK-801 in rat brain by chronic infusion of subtoxic dose of MK-801 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T08%3A29%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Modulation%20of%20the%20levels%20of%20NMDA%20receptor%20subunit%20mRNA%20and%20the%20bindings%20of%20%5B3H%5DMK-801%20in%20rat%20brain%20by%20chronic%20infusion%20of%20subtoxic%20dose%20of%20MK-801&rft.jtitle=Neurochemical%20research&rft.au=OH,%20Seikwan&rft.date=2001-05-01&rft.volume=26&rft.issue=5&rft.spage=559&rft.epage=565&rft.pages=559-565&rft.issn=0364-3190&rft.eissn=1573-6903&rft.coden=NEREDZ&rft_id=info:doi/10.1023/A:1010977315838&rft_dat=%3Cproquest_pubme%3E390357841%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=221804158&rft_id=info:pmid/11513485&rfr_iscdi=true |