Failure of female baboons (Papio anubis) to conceive following immunization with recombinant non-human primate zona pellucida glycoprotein-B expressed in Escherichia coli
Progress in the development of an immunocontraceptive vaccine based on zona pellucida glycoproteins has been hampered due to observed ovarian dysfunction associated with immunization using these as immunogens. In this study four female baboons (Papio anubis) were immunized with recombinant bonnet mo...
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Veröffentlicht in: | Vaccine 2000-07, Vol.18 (26), p.2970-2978 |
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description | Progress in the development of an immunocontraceptive vaccine based on zona pellucida glycoproteins has been hampered due to observed ovarian dysfunction associated with immunization using these as immunogens. In this study four female baboons (Papio anubis) were immunized with recombinant bonnet monkey (Macaca radiata) zona pellucida glycoprotein-B (r-bmZPB) expressed in Escherichia coli and conjugated to diphtheria toxoid (DT) using Arlacel-A and Squalene as adjuvants. All the immunized animals elicited a good antibody response against r-bmZPB, continued to have ovulatory cycles and showed no disturbance in the cyclicity. In presence of high titres of circulating anti-bmZPB antibodies (>2×103 antibody units), the immunized animals failed to conceive following mating with males of proven fertility. Pregnancy was observed in the immunized animals subsequent to the decline in anti-r-bmZPB antibody titres. These results, though preliminary, suggest that immunization with ZPB may be used for immunocontraception without obvious ovarian dysfunction. |
doi_str_mv | 10.1016/S0264-410X(00)00103-1 |
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In this study four female baboons (Papio anubis) were immunized with recombinant bonnet monkey (Macaca radiata) zona pellucida glycoprotein-B (r-bmZPB) expressed in Escherichia coli and conjugated to diphtheria toxoid (DT) using Arlacel-A and Squalene as adjuvants. All the immunized animals elicited a good antibody response against r-bmZPB, continued to have ovulatory cycles and showed no disturbance in the cyclicity. In presence of high titres of circulating anti-bmZPB antibodies (>2×103 antibody units), the immunized animals failed to conceive following mating with males of proven fertility. Pregnancy was observed in the immunized animals subsequent to the decline in anti-r-bmZPB antibody titres. These results, though preliminary, suggest that immunization with ZPB may be used for immunocontraception without obvious ovarian dysfunction.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(00)00103-1</identifier><identifier>PMID: 10825598</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adjuvants ; Animals ; Antibodies ; Antibodies - analysis ; Antibody response ; Baboons ; Bacteria ; Biological and medical sciences ; Birth control ; Cloning ; Contraception ; Diphtheria ; E coli ; Egg Proteins - immunology ; Escherichia coli ; Escherichia coli - genetics ; Female ; Females ; Fertility ; glycoprotein B ; Glycoproteins ; Gynecology. Andrology. Obstetrics ; Immunization ; Immunocontraception ; Immunogenicity ; Immunologic contraceptives ; Immunology ; Laboratories ; Macaca radiata ; Medical sciences ; Membrane Glycoproteins - immunology ; Menstrual Cycle ; Menstruation ; Monkeys & apes ; Other methods of contraception. Sterilization ; Ovulation ; Papio ; Papio anubis ; Pregnancy ; Proteins ; R&D ; Rabbits ; Receptors, Cell Surface ; Recombinant Proteins - immunology ; Recombinant zona pellucida glycoprotein-B ; Research & development ; Squalene ; Zona pellucida ; Zona Pellucida - immunology ; Zona Pellucida Glycoproteins</subject><ispartof>Vaccine, 2000-07, Vol.18 (26), p.2970-2978</ispartof><rights>2000 Elsevier Science Ltd</rights><rights>2000 INIST-CNRS</rights><rights>2000. Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-24df769286bb89342718f5fc94b6f5a8ca203420d54612820249f718cbedb0a93</citedby><cites>FETCH-LOGICAL-c449t-24df769286bb89342718f5fc94b6f5a8ca203420d54612820249f718cbedb0a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2198003495?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,64361,64363,64365,65309,72215</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1443370$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10825598$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Govind, Chhabi K</creatorcontrib><creatorcontrib>Gupta, Satish K</creatorcontrib><title>Failure of female baboons (Papio anubis) to conceive following immunization with recombinant non-human primate zona pellucida glycoprotein-B expressed in Escherichia coli</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Progress in the development of an immunocontraceptive vaccine based on zona pellucida glycoproteins has been hampered due to observed ovarian dysfunction associated with immunization using these as immunogens. In this study four female baboons (Papio anubis) were immunized with recombinant bonnet monkey (Macaca radiata) zona pellucida glycoprotein-B (r-bmZPB) expressed in Escherichia coli and conjugated to diphtheria toxoid (DT) using Arlacel-A and Squalene as adjuvants. All the immunized animals elicited a good antibody response against r-bmZPB, continued to have ovulatory cycles and showed no disturbance in the cyclicity. In presence of high titres of circulating anti-bmZPB antibodies (>2×103 antibody units), the immunized animals failed to conceive following mating with males of proven fertility. Pregnancy was observed in the immunized animals subsequent to the decline in anti-r-bmZPB antibody titres. These results, though preliminary, suggest that immunization with ZPB may be used for immunocontraception without obvious ovarian dysfunction.</description><subject>Adjuvants</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies - analysis</subject><subject>Antibody response</subject><subject>Baboons</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Cloning</subject><subject>Contraception</subject><subject>Diphtheria</subject><subject>E coli</subject><subject>Egg Proteins - immunology</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Female</subject><subject>Females</subject><subject>Fertility</subject><subject>glycoprotein B</subject><subject>Glycoproteins</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Immunization</subject><subject>Immunocontraception</subject><subject>Immunogenicity</subject><subject>Immunologic contraceptives</subject><subject>Immunology</subject><subject>Laboratories</subject><subject>Macaca radiata</subject><subject>Medical sciences</subject><subject>Membrane Glycoproteins - immunology</subject><subject>Menstrual Cycle</subject><subject>Menstruation</subject><subject>Monkeys & apes</subject><subject>Other methods of contraception. Sterilization</subject><subject>Ovulation</subject><subject>Papio</subject><subject>Papio anubis</subject><subject>Pregnancy</subject><subject>Proteins</subject><subject>R&D</subject><subject>Rabbits</subject><subject>Receptors, Cell Surface</subject><subject>Recombinant Proteins - immunology</subject><subject>Recombinant zona pellucida glycoprotein-B</subject><subject>Research & development</subject><subject>Squalene</subject><subject>Zona pellucida</subject><subject>Zona Pellucida - immunology</subject><subject>Zona Pellucida Glycoproteins</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkd1qFjEQhhdR7Gf1EpSAIu3B6iSb_cmRaGlVKCio4FnIZmf7TdlN1mS3tb0kr9J8P6h40qPA8MzknXmy7CmHVxx49foLiErmksP3I4BjAA5Fzu9lK97URS5K3tzPVn-Qg-xRjJcAUBZcPcwOODSiLFWzyn6dGRqWgMz3rMfRDMha03rvIjv6bCbyzLilpXjMZs-sdxbpClnvh8Ffk7tgNI6Lo1szk3fsmuY1C2j92JIzbmbOu3y9jMaxKdBoZmS33hk24TAsljrDLoYb66fgZySXv2P4cwoYI3aMHDuNdo2B7JpM-nmgx9mD3gwRn-zfw-zb2enXkw_5-af3H0_enudWSjXnQnZ9XSnRVG3bqEKKmjd92Vsl26ovTWONgFSFrpQVF40AIVWfGNti14JRxWH2cjc35fqxYJz1SNGmyMahX6KuOU_tNdwJ8roCBXIz8fl_4KVfgktLaMFVAymPKhNV7igbfIwBe709WrjRHPTGud461xuhGkBvnWue-p7tpy_tiN0_XTvJCXixB0y0ZuiDcZbiX07Kotiu82aHYTruFWHQ0RIm4x0lp7PuPN2R5DfVl8qA</recordid><startdate>20000701</startdate><enddate>20000701</enddate><creator>Govind, Chhabi K</creator><creator>Gupta, Satish K</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20000701</creationdate><title>Failure of female baboons (Papio anubis) to conceive following immunization with recombinant non-human primate zona pellucida glycoprotein-B expressed in Escherichia coli</title><author>Govind, Chhabi K ; Gupta, Satish K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-24df769286bb89342718f5fc94b6f5a8ca203420d54612820249f718cbedb0a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adjuvants</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies - analysis</topic><topic>Antibody response</topic><topic>Baboons</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Cloning</topic><topic>Contraception</topic><topic>Diphtheria</topic><topic>E coli</topic><topic>Egg Proteins - immunology</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Female</topic><topic>Females</topic><topic>Fertility</topic><topic>glycoprotein B</topic><topic>Glycoproteins</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Immunization</topic><topic>Immunocontraception</topic><topic>Immunogenicity</topic><topic>Immunologic contraceptives</topic><topic>Immunology</topic><topic>Laboratories</topic><topic>Macaca radiata</topic><topic>Medical sciences</topic><topic>Membrane Glycoproteins - immunology</topic><topic>Menstrual Cycle</topic><topic>Menstruation</topic><topic>Monkeys & apes</topic><topic>Other methods of contraception. Sterilization</topic><topic>Ovulation</topic><topic>Papio</topic><topic>Papio anubis</topic><topic>Pregnancy</topic><topic>Proteins</topic><topic>R&D</topic><topic>Rabbits</topic><topic>Receptors, Cell Surface</topic><topic>Recombinant Proteins - immunology</topic><topic>Recombinant zona pellucida glycoprotein-B</topic><topic>Research & development</topic><topic>Squalene</topic><topic>Zona pellucida</topic><topic>Zona Pellucida - immunology</topic><topic>Zona Pellucida Glycoproteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Govind, Chhabi K</creatorcontrib><creatorcontrib>Gupta, Satish K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Govind, Chhabi K</au><au>Gupta, Satish K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Failure of female baboons (Papio anubis) to conceive following immunization with recombinant non-human primate zona pellucida glycoprotein-B expressed in Escherichia coli</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2000-07-01</date><risdate>2000</risdate><volume>18</volume><issue>26</issue><spage>2970</spage><epage>2978</epage><pages>2970-2978</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Progress in the development of an immunocontraceptive vaccine based on zona pellucida glycoproteins has been hampered due to observed ovarian dysfunction associated with immunization using these as immunogens. In this study four female baboons (Papio anubis) were immunized with recombinant bonnet monkey (Macaca radiata) zona pellucida glycoprotein-B (r-bmZPB) expressed in Escherichia coli and conjugated to diphtheria toxoid (DT) using Arlacel-A and Squalene as adjuvants. All the immunized animals elicited a good antibody response against r-bmZPB, continued to have ovulatory cycles and showed no disturbance in the cyclicity. In presence of high titres of circulating anti-bmZPB antibodies (>2×103 antibody units), the immunized animals failed to conceive following mating with males of proven fertility. Pregnancy was observed in the immunized animals subsequent to the decline in anti-r-bmZPB antibody titres. These results, though preliminary, suggest that immunization with ZPB may be used for immunocontraception without obvious ovarian dysfunction.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>10825598</pmid><doi>10.1016/S0264-410X(00)00103-1</doi><tpages>9</tpages></addata></record> |
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subjects | Adjuvants Animals Antibodies Antibodies - analysis Antibody response Baboons Bacteria Biological and medical sciences Birth control Cloning Contraception Diphtheria E coli Egg Proteins - immunology Escherichia coli Escherichia coli - genetics Female Females Fertility glycoprotein B Glycoproteins Gynecology. Andrology. Obstetrics Immunization Immunocontraception Immunogenicity Immunologic contraceptives Immunology Laboratories Macaca radiata Medical sciences Membrane Glycoproteins - immunology Menstrual Cycle Menstruation Monkeys & apes Other methods of contraception. Sterilization Ovulation Papio Papio anubis Pregnancy Proteins R&D Rabbits Receptors, Cell Surface Recombinant Proteins - immunology Recombinant zona pellucida glycoprotein-B Research & development Squalene Zona pellucida Zona Pellucida - immunology Zona Pellucida Glycoproteins |
title | Failure of female baboons (Papio anubis) to conceive following immunization with recombinant non-human primate zona pellucida glycoprotein-B expressed in Escherichia coli |
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