Trans-10, Cis-12, But Not Cis-9, Trans-11, Conjugated Linoleic Acid Attenuates Lipogenesis in Primary Cultures of Stromal Vascular Cells from Human Adipose Tissue
We have previously shown that both a commercially available mixture of conjugated linoleic acid (CLA) isomers and the trans-10, cis-12 isomer of CLA reduced the triglyceride (TG) content and induced apoptosis in differentiating cultures of murine 3T3-L1 preadipocytes. However, the influence of CLA i...
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| Veröffentlicht in: | The Journal of nutrition 2001-09, Vol.131 (9), p.2316-2321 |
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| creator | Brown, J.M. Halvorsen, Y.D. Lea-Currie, Y.R. Geigerman, C. McIntosh, M. |
| description | We have previously shown that both a commercially available mixture of conjugated linoleic acid (CLA) isomers and the trans-10, cis-12 isomer of CLA reduced the triglyceride (TG) content and induced apoptosis in differentiating cultures of murine 3T3-L1 preadipocytes. However, the influence of CLA isomers on differentiating human (pre)adipocytes is unknown. Therefore, we conducted a series of studies using primary cultures of stromal vascular cells isolated from human adipose tissue to determine: 1) the influence of seeding density and thiazolidinedione (TZD) concentration on TG content; 2) the chronic dose response of cis-9, trans-11 CLA vs. trans-10, cis-12 CLA on TG content; 3) whether chronic linoleic acid supplementation could rescue the TG content of CLA-treated cultures; and 4) whether trans-10, cis-12-mediated reduction in cellular TG was due to decreased lipogenesis and/or increased lipolysis. In expt. 1, the TG content [μmol/(L · 106 cells)] increased as both seeding density and TZD concentration increased. For example, cultures seeded at 4 × 104 cells/cm2 and supplemented with 10 μmol/L BRL 49653 had 10-fold more TG than similarly seeded cultures without BRL 49653. In expt. 2, TG content decreased as the level of trans-10, cis-12 CLA increased from 1 to 10 μmol/L, whereas the TG content increased with increasing concentrations of either linoleic acid or cis-9, trans-11 CLA. In expt. 3, linoleic acid supplementation restored the TG content of cultures treated with trans-10, cis-12 CLA compared with cultures treated with CLA alone, suggesting that attenuation of TG content by CLA is reversible. In expt. 4, glucose incorporation into total lipid decreased with increasing levels of trans-10, cis-12 CLA, whereas neither CLA isomer acutely affected lipolysis. These data suggest that the reported antiobesity actions of a supplement containing a crude mixture of CLA isomers given to humans may be due to inhibition of lipogenesis by the trans-10, cis-12 isomer. J. Nutr. 131: 2316–2321, 2001. |
| doi_str_mv | 10.1093/jn/131.9.2316 |
| format | Article |
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However, the influence of CLA isomers on differentiating human (pre)adipocytes is unknown. Therefore, we conducted a series of studies using primary cultures of stromal vascular cells isolated from human adipose tissue to determine: 1) the influence of seeding density and thiazolidinedione (TZD) concentration on TG content; 2) the chronic dose response of cis-9, trans-11 CLA vs. trans-10, cis-12 CLA on TG content; 3) whether chronic linoleic acid supplementation could rescue the TG content of CLA-treated cultures; and 4) whether trans-10, cis-12-mediated reduction in cellular TG was due to decreased lipogenesis and/or increased lipolysis. In expt. 1, the TG content [μmol/(L · 106 cells)] increased as both seeding density and TZD concentration increased. For example, cultures seeded at 4 × 104 cells/cm2 and supplemented with 10 μmol/L BRL 49653 had 10-fold more TG than similarly seeded cultures without BRL 49653. In expt. 2, TG content decreased as the level of trans-10, cis-12 CLA increased from 1 to 10 μmol/L, whereas the TG content increased with increasing concentrations of either linoleic acid or cis-9, trans-11 CLA. In expt. 3, linoleic acid supplementation restored the TG content of cultures treated with trans-10, cis-12 CLA compared with cultures treated with CLA alone, suggesting that attenuation of TG content by CLA is reversible. In expt. 4, glucose incorporation into total lipid decreased with increasing levels of trans-10, cis-12 CLA, whereas neither CLA isomer acutely affected lipolysis. These data suggest that the reported antiobesity actions of a supplement containing a crude mixture of CLA isomers given to humans may be due to inhibition of lipogenesis by the trans-10, cis-12 isomer. J. Nutr. 131: 2316–2321, 2001.</description><identifier>ISSN: 0022-3166</identifier><identifier>EISSN: 1541-6100</identifier><identifier>DOI: 10.1093/jn/131.9.2316</identifier><identifier>PMID: 11533273</identifier><identifier>CODEN: JONUAI</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>adipose tissue ; Adipose Tissue - blood supply ; Biological and medical sciences ; Blood Vessels - drug effects ; Blood Vessels - metabolism ; Body fat ; Cells, Cultured ; conjugated linoleic acid ; connective tissues ; cultured cells ; differentiation ; dose response ; Dose-Response Relationship, Drug ; Fatty acids ; Fundamental and applied biological sciences. Psychology ; Glucose - metabolism ; human (pre)adipocytes ; human nutrition ; human physiology ; Humans ; isomers ; Linoleic Acid - metabolism ; Linoleic Acid - pharmacology ; lipid content ; Lipids - biosynthesis ; Lipids. Glycolipids ; lipogenesis ; Lipolysis ; Metabolisms and neurohumoral controls ; methodology ; Nutrition ; Obesity ; Osmolar Concentration ; Stereoisomerism ; Stromal Cells - drug effects ; Stromal Cells - metabolism ; Thiazoles - pharmacology ; Thiazolidinediones ; triacylglycerols ; triglyceride ; Triglycerides - metabolism ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>The Journal of nutrition, 2001-09, Vol.131 (9), p.2316-2321</ispartof><rights>2001 American Society for Nutrition.</rights><rights>2002 INIST-CNRS</rights><rights>Copyright American Institute of Nutrition Sep 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-cd86cc75bd6fc8063299b4c4353da6b3a2c09353c774db40b62ea583cb93ffbd3</citedby><cites>FETCH-LOGICAL-c457t-cd86cc75bd6fc8063299b4c4353da6b3a2c09353c774db40b62ea583cb93ffbd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,780,784,789,790,23928,23929,25138,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14143412$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11533273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brown, J.M.</creatorcontrib><creatorcontrib>Halvorsen, Y.D.</creatorcontrib><creatorcontrib>Lea-Currie, Y.R.</creatorcontrib><creatorcontrib>Geigerman, C.</creatorcontrib><creatorcontrib>McIntosh, M.</creatorcontrib><title>Trans-10, Cis-12, But Not Cis-9, Trans-11, Conjugated Linoleic Acid Attenuates Lipogenesis in Primary Cultures of Stromal Vascular Cells from Human Adipose Tissue</title><title>The Journal of nutrition</title><addtitle>J Nutr</addtitle><description>We have previously shown that both a commercially available mixture of conjugated linoleic acid (CLA) isomers and the trans-10, cis-12 isomer of CLA reduced the triglyceride (TG) content and induced apoptosis in differentiating cultures of murine 3T3-L1 preadipocytes. However, the influence of CLA isomers on differentiating human (pre)adipocytes is unknown. Therefore, we conducted a series of studies using primary cultures of stromal vascular cells isolated from human adipose tissue to determine: 1) the influence of seeding density and thiazolidinedione (TZD) concentration on TG content; 2) the chronic dose response of cis-9, trans-11 CLA vs. trans-10, cis-12 CLA on TG content; 3) whether chronic linoleic acid supplementation could rescue the TG content of CLA-treated cultures; and 4) whether trans-10, cis-12-mediated reduction in cellular TG was due to decreased lipogenesis and/or increased lipolysis. In expt. 1, the TG content [μmol/(L · 106 cells)] increased as both seeding density and TZD concentration increased. For example, cultures seeded at 4 × 104 cells/cm2 and supplemented with 10 μmol/L BRL 49653 had 10-fold more TG than similarly seeded cultures without BRL 49653. In expt. 2, TG content decreased as the level of trans-10, cis-12 CLA increased from 1 to 10 μmol/L, whereas the TG content increased with increasing concentrations of either linoleic acid or cis-9, trans-11 CLA. In expt. 3, linoleic acid supplementation restored the TG content of cultures treated with trans-10, cis-12 CLA compared with cultures treated with CLA alone, suggesting that attenuation of TG content by CLA is reversible. In expt. 4, glucose incorporation into total lipid decreased with increasing levels of trans-10, cis-12 CLA, whereas neither CLA isomer acutely affected lipolysis. These data suggest that the reported antiobesity actions of a supplement containing a crude mixture of CLA isomers given to humans may be due to inhibition of lipogenesis by the trans-10, cis-12 isomer. J. Nutr. 131: 2316–2321, 2001.</description><subject>adipose tissue</subject><subject>Adipose Tissue - blood supply</subject><subject>Biological and medical sciences</subject><subject>Blood Vessels - drug effects</subject><subject>Blood Vessels - metabolism</subject><subject>Body fat</subject><subject>Cells, Cultured</subject><subject>conjugated linoleic acid</subject><subject>connective tissues</subject><subject>cultured cells</subject><subject>differentiation</subject><subject>dose response</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fatty acids</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - metabolism</subject><subject>human (pre)adipocytes</subject><subject>human nutrition</subject><subject>human physiology</subject><subject>Humans</subject><subject>isomers</subject><subject>Linoleic Acid - metabolism</subject><subject>Linoleic Acid - pharmacology</subject><subject>lipid content</subject><subject>Lipids - biosynthesis</subject><subject>Lipids. Glycolipids</subject><subject>lipogenesis</subject><subject>Lipolysis</subject><subject>Metabolisms and neurohumoral controls</subject><subject>methodology</subject><subject>Nutrition</subject><subject>Obesity</subject><subject>Osmolar Concentration</subject><subject>Stereoisomerism</subject><subject>Stromal Cells - drug effects</subject><subject>Stromal Cells - metabolism</subject><subject>Thiazoles - pharmacology</subject><subject>Thiazolidinediones</subject><subject>triacylglycerols</subject><subject>triglyceride</subject><subject>Triglycerides - metabolism</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0022-3166</issn><issn>1541-6100</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10VGLEzEQB_BFFK-ePvqqQdCnbi-TZHe7j7WoJxQVrudryCbZkmWb1GQj-HX8pE7twoHg05DMj2GYf1G8BLoC2vKbwd8Ah1W7YhzqR8UCKgFlDZQ-LhaUMlbid31VPEtpoJSCaNdPiyuAinPW8EXxex-VTyXQJdk6rGxJ3ueJfAnT33e7JDMABMEP-aAma8jO-TBap8lGO0M202R9xkbCxikcrLfJJeI8-RbdUcVfZJvHKUfsh57cTTEc1Ui-q6TzqCLZ2nFMpMdfcpuPypONwSnJkr1LKdvnxZNejcm-mOt1cf_xw357W-6-fvq83exKLapmKrVZ11o3VWfqXq9pzVnbdkILXnGj6o4rpvFeFddNI0wnaFczq6o1113L-74z_Lp4d5l7iuFHtmmSR5c07qa8DTnJBkBAyzjCN__AIeTocTcJbSMqaOgZlRekY0gp2l6eLreQQOU5OTl4icnJVp6TQ_9qHpq7ozUPeo4KwdsZ4N3U2GMs2qUHJ0BwAQzd64vrVZDqENHc3zEKnALllK4piuYiLF7zp7NRJu2s19a4aPUkTXD_WfIPCui7QA</recordid><startdate>20010901</startdate><enddate>20010901</enddate><creator>Brown, J.M.</creator><creator>Halvorsen, Y.D.</creator><creator>Lea-Currie, Y.R.</creator><creator>Geigerman, C.</creator><creator>McIntosh, M.</creator><general>Elsevier Inc</general><general>American Society for Nutritional Sciences</general><general>American Institute of Nutrition</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20010901</creationdate><title>Trans-10, Cis-12, But Not Cis-9, Trans-11, Conjugated Linoleic Acid Attenuates Lipogenesis in Primary Cultures of Stromal Vascular Cells from Human Adipose Tissue</title><author>Brown, J.M. ; Halvorsen, Y.D. ; Lea-Currie, Y.R. ; Geigerman, C. ; McIntosh, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-cd86cc75bd6fc8063299b4c4353da6b3a2c09353c774db40b62ea583cb93ffbd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>adipose tissue</topic><topic>Adipose Tissue - blood supply</topic><topic>Biological and medical sciences</topic><topic>Blood Vessels - drug effects</topic><topic>Blood Vessels - metabolism</topic><topic>Body fat</topic><topic>Cells, Cultured</topic><topic>conjugated linoleic acid</topic><topic>connective tissues</topic><topic>cultured cells</topic><topic>differentiation</topic><topic>dose response</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fatty acids</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - metabolism</topic><topic>human (pre)adipocytes</topic><topic>human nutrition</topic><topic>human physiology</topic><topic>Humans</topic><topic>isomers</topic><topic>Linoleic Acid - metabolism</topic><topic>Linoleic Acid - pharmacology</topic><topic>lipid content</topic><topic>Lipids - biosynthesis</topic><topic>Lipids. Glycolipids</topic><topic>lipogenesis</topic><topic>Lipolysis</topic><topic>Metabolisms and neurohumoral controls</topic><topic>methodology</topic><topic>Nutrition</topic><topic>Obesity</topic><topic>Osmolar Concentration</topic><topic>Stereoisomerism</topic><topic>Stromal Cells - drug effects</topic><topic>Stromal Cells - metabolism</topic><topic>Thiazoles - pharmacology</topic><topic>Thiazolidinediones</topic><topic>triacylglycerols</topic><topic>triglyceride</topic><topic>Triglycerides - metabolism</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, J.M.</creatorcontrib><creatorcontrib>Halvorsen, Y.D.</creatorcontrib><creatorcontrib>Lea-Currie, Y.R.</creatorcontrib><creatorcontrib>Geigerman, C.</creatorcontrib><creatorcontrib>McIntosh, M.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, J.M.</au><au>Halvorsen, Y.D.</au><au>Lea-Currie, Y.R.</au><au>Geigerman, C.</au><au>McIntosh, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Trans-10, Cis-12, But Not Cis-9, Trans-11, Conjugated Linoleic Acid Attenuates Lipogenesis in Primary Cultures of Stromal Vascular Cells from Human Adipose Tissue</atitle><jtitle>The Journal of nutrition</jtitle><addtitle>J Nutr</addtitle><date>2001-09-01</date><risdate>2001</risdate><volume>131</volume><issue>9</issue><spage>2316</spage><epage>2321</epage><pages>2316-2321</pages><issn>0022-3166</issn><eissn>1541-6100</eissn><coden>JONUAI</coden><abstract>We have previously shown that both a commercially available mixture of conjugated linoleic acid (CLA) isomers and the trans-10, cis-12 isomer of CLA reduced the triglyceride (TG) content and induced apoptosis in differentiating cultures of murine 3T3-L1 preadipocytes. However, the influence of CLA isomers on differentiating human (pre)adipocytes is unknown. Therefore, we conducted a series of studies using primary cultures of stromal vascular cells isolated from human adipose tissue to determine: 1) the influence of seeding density and thiazolidinedione (TZD) concentration on TG content; 2) the chronic dose response of cis-9, trans-11 CLA vs. trans-10, cis-12 CLA on TG content; 3) whether chronic linoleic acid supplementation could rescue the TG content of CLA-treated cultures; and 4) whether trans-10, cis-12-mediated reduction in cellular TG was due to decreased lipogenesis and/or increased lipolysis. In expt. 1, the TG content [μmol/(L · 106 cells)] increased as both seeding density and TZD concentration increased. For example, cultures seeded at 4 × 104 cells/cm2 and supplemented with 10 μmol/L BRL 49653 had 10-fold more TG than similarly seeded cultures without BRL 49653. In expt. 2, TG content decreased as the level of trans-10, cis-12 CLA increased from 1 to 10 μmol/L, whereas the TG content increased with increasing concentrations of either linoleic acid or cis-9, trans-11 CLA. In expt. 3, linoleic acid supplementation restored the TG content of cultures treated with trans-10, cis-12 CLA compared with cultures treated with CLA alone, suggesting that attenuation of TG content by CLA is reversible. In expt. 4, glucose incorporation into total lipid decreased with increasing levels of trans-10, cis-12 CLA, whereas neither CLA isomer acutely affected lipolysis. These data suggest that the reported antiobesity actions of a supplement containing a crude mixture of CLA isomers given to humans may be due to inhibition of lipogenesis by the trans-10, cis-12 isomer. J. Nutr. 131: 2316–2321, 2001.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>11533273</pmid><doi>10.1093/jn/131.9.2316</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | adipose tissue Adipose Tissue - blood supply Biological and medical sciences Blood Vessels - drug effects Blood Vessels - metabolism Body fat Cells, Cultured conjugated linoleic acid connective tissues cultured cells differentiation dose response Dose-Response Relationship, Drug Fatty acids Fundamental and applied biological sciences. Psychology Glucose - metabolism human (pre)adipocytes human nutrition human physiology Humans isomers Linoleic Acid - metabolism Linoleic Acid - pharmacology lipid content Lipids - biosynthesis Lipids. Glycolipids lipogenesis Lipolysis Metabolisms and neurohumoral controls methodology Nutrition Obesity Osmolar Concentration Stereoisomerism Stromal Cells - drug effects Stromal Cells - metabolism Thiazoles - pharmacology Thiazolidinediones triacylglycerols triglyceride Triglycerides - metabolism Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Trans-10, Cis-12, But Not Cis-9, Trans-11, Conjugated Linoleic Acid Attenuates Lipogenesis in Primary Cultures of Stromal Vascular Cells from Human Adipose Tissue |
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