Comparison of culture, PCR and immunoassays for detecting Escherichia coli O157 following enrichment culture and immunomagnetic separation performed on naturally contaminated raw meat products

The aims of this study were (i) to evaluate the specificity and sensitivity of three previously described PCR assays for the detection of Escherichia coli O157 and, (ii) to compare PCR, culture, and two visual immunoassays (VIAs), BioSign™ and Path-Stik™, for detecting E. coli O157 after enrichment...

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Veröffentlicht in:	International journal of food microbiology 2001-08, Vol.68 (1), p.11-20
Hauptverfasser:	Chapman, P.A, Ellin, M, Ashton, R, Shafique, W
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cattle Colony Count, Microbial Culture Media Enrichment culture Escherichia coli Escherichia coli O157 Escherichia coli O157 - isolation & purification Food industries Food microbiology Fundamental and applied biological sciences. Psychology Immunoassay Immunomagnetic Separation Meat and meat product industries Meat Products - microbiology Polymerase Chain Reaction Raw meat products Sensitivity and Specificity Sheep
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