pH Dependence of the Inwardly Rectifying Potassium Channel, Kir5.1, and Localization in Renal Tubular Epithelia
The physiological role of the inwardly rectifying potassium channel, Kir5.1, is poorly understood, as is the molecular identity of many renal potassium channels. In this study we have used Kir5.1-specific antibodies to reveal abundant expression of Kir5.1 in renal tubular epithelial cells, where Kir...
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| Veröffentlicht in: | The Journal of biological chemistry 2000-06, Vol.275 (22), p.16404-16407 |
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| container_issue | 22 |
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| container_title | The Journal of biological chemistry |
| container_volume | 275 |
| creator | Tucker, Stephen J. Imbrici, Paola Salvatore, Lorena D'Adamo, Maria Cristina Pessia, Mauro |
| description | The physiological role of the inwardly rectifying potassium channel, Kir5.1, is poorly understood, as is the molecular identity of many renal potassium channels. In this study we have used Kir5.1-specific antibodies to reveal abundant expression of Kir5.1 in renal tubular epithelial cells, where Kir4.1 is also expressed. Moreover, we also show that Kir5.1/Kir4.1 heteromeric channel activity is extremely sensitive to inhibition by intracellular acidification and that this novel property is conferred predominantly by the Kir5.1 subunit. These findings suggest that Kir5.1/Kir4.1 heteromeric channels are likely to exist in vivo and implicate an important and novel functional role for the Kir5.1 subunit. |
| doi_str_mv | 10.1074/jbc.C000127200 |
| format | Article |
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In this study we have used Kir5.1-specific antibodies to reveal abundant expression of Kir5.1 in renal tubular epithelial cells, where Kir4.1 is also expressed. Moreover, we also show that Kir5.1/Kir4.1 heteromeric channel activity is extremely sensitive to inhibition by intracellular acidification and that this novel property is conferred predominantly by the Kir5.1 subunit. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Base Sequence DNA Primers Epithelium - metabolism Hydrogen-Ion Concentration Immunohistochemistry Kidney Tubules - metabolism Molecular Sequence Data Potassium Channels - metabolism Potassium Channels, Inwardly Rectifying Rats |
| title | pH Dependence of the Inwardly Rectifying Potassium Channel, Kir5.1, and Localization in Renal Tubular Epithelia |
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