Expression, production, and secretion of vascular endothelial growth factor and interleukin-6 by granulosa cells is comparable in women with and without endometriosis
Objective: To investigate the production and secretion of interleukin (IL)-6 and vascular endothelial growth factor (VEGF) mRNA and protein by granulosa luteal cells (GCs) in vivo and in vitro in women with and without endometriosis. Design: Prospective study. Setting: A private, university-affiliat...
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| Veröffentlicht in: | Fertility and sterility 2001-09, Vol.76 (3), p.568-575 |
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| creator | Garrido, Nicolás Albert, Carmela Krüssel, Jan S O’Connor, José E Remohı́, José Simón, Carlos Pellicer, Antonio |
| description | Objective: To investigate the production and secretion of interleukin (IL)-6 and vascular endothelial growth factor (VEGF) mRNA and protein by granulosa luteal cells (GCs) in vivo and in vitro in women with and without endometriosis.
Design: Prospective study.
Setting: A private, university-affiliated assisted reproduction unit and a university center.
Patient(s): Women with severe endometriosis (n = 6) or without the disease (n = 14) after laparoscopy, undergoing in vitro fertilization/intracytoplasmic sperm injection and embryo transfer.
Intervention(s): GCs were obtained from each aspirate.
Main Outcome Measure(s): Intracellular and secreted protein, as well as mRNA for both VEGF and IL-6 in GCs.
Result(s): The expression of VEGF and IL-6 mRNAs in vivo and in vitro was similar in both groups. Also, GCs from patients with endometriosis produced and secreted equal amounts of these proteins compared with controls without the disease, either in freshly isolated cells or in 24-hour cultures.
Conclusion(s): The GC function in terms of VEGF and IL-6 production does not seem to be altered in patients with endometriosis in comparison with those without this condition. |
| doi_str_mv | 10.1016/S0015-0282(01)01961-6 |
| format | Article |
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Design: Prospective study.
Setting: A private, university-affiliated assisted reproduction unit and a university center.
Patient(s): Women with severe endometriosis (n = 6) or without the disease (n = 14) after laparoscopy, undergoing in vitro fertilization/intracytoplasmic sperm injection and embryo transfer.
Intervention(s): GCs were obtained from each aspirate.
Main Outcome Measure(s): Intracellular and secreted protein, as well as mRNA for both VEGF and IL-6 in GCs.
Result(s): The expression of VEGF and IL-6 mRNAs in vivo and in vitro was similar in both groups. Also, GCs from patients with endometriosis produced and secreted equal amounts of these proteins compared with controls without the disease, either in freshly isolated cells or in 24-hour cultures.
Conclusion(s): The GC function in terms of VEGF and IL-6 production does not seem to be altered in patients with endometriosis in comparison with those without this condition.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(01)01961-6</identifier><identifier>PMID: 11532483</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Biological and medical sciences ; Cells, Cultured ; Culture Media, Conditioned ; DNA Primers ; Embryo Transfer ; Endometriosis ; Endometriosis - genetics ; Endometriosis - physiopathology ; Endothelial Growth Factors - genetics ; Endothelial Growth Factors - metabolism ; Enzyme-Linked Immunosorbent Assay ; Female ; Female genital diseases ; Fertilization in Vitro ; Flow Cytometry ; follicular environment ; granulosa cells ; Granulosa Cells - physiology ; Gynecology. Andrology. Obstetrics ; Humans ; interleukin-6 (IL-6) ; Interleukin-6 - genetics ; Interleukin-6 - metabolism ; Lymphokines - genetics ; Lymphokines - metabolism ; Medical sciences ; Non tumoral diseases ; oocyte quality ; Polymerase Chain Reaction ; Reference Values ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Sperm Injections, Intracytoplasmic ; Transcription, Genetic ; vascular endothelial growth factor (VEGF) ; Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors</subject><ispartof>Fertility and sterility, 2001-09, Vol.76 (3), p.568-575</ispartof><rights>2001 American Society for Reproductive Medicine</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-405f51bcc03ad6a980957b56da6fd7926dd33bacb2c7881db045046be9fc05663</citedby><cites>FETCH-LOGICAL-c438t-405f51bcc03ad6a980957b56da6fd7926dd33bacb2c7881db045046be9fc05663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0015-0282(01)01961-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14061521$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11532483$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garrido, Nicolás</creatorcontrib><creatorcontrib>Albert, Carmela</creatorcontrib><creatorcontrib>Krüssel, Jan S</creatorcontrib><creatorcontrib>O’Connor, José E</creatorcontrib><creatorcontrib>Remohı́, José</creatorcontrib><creatorcontrib>Simón, Carlos</creatorcontrib><creatorcontrib>Pellicer, Antonio</creatorcontrib><title>Expression, production, and secretion of vascular endothelial growth factor and interleukin-6 by granulosa cells is comparable in women with and without endometriosis</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective: To investigate the production and secretion of interleukin (IL)-6 and vascular endothelial growth factor (VEGF) mRNA and protein by granulosa luteal cells (GCs) in vivo and in vitro in women with and without endometriosis.
Design: Prospective study.
Setting: A private, university-affiliated assisted reproduction unit and a university center.
Patient(s): Women with severe endometriosis (n = 6) or without the disease (n = 14) after laparoscopy, undergoing in vitro fertilization/intracytoplasmic sperm injection and embryo transfer.
Intervention(s): GCs were obtained from each aspirate.
Main Outcome Measure(s): Intracellular and secreted protein, as well as mRNA for both VEGF and IL-6 in GCs.
Result(s): The expression of VEGF and IL-6 mRNAs in vivo and in vitro was similar in both groups. Also, GCs from patients with endometriosis produced and secreted equal amounts of these proteins compared with controls without the disease, either in freshly isolated cells or in 24-hour cultures.
Conclusion(s): The GC function in terms of VEGF and IL-6 production does not seem to be altered in patients with endometriosis in comparison with those without this condition.</description><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Culture Media, Conditioned</subject><subject>DNA Primers</subject><subject>Embryo Transfer</subject><subject>Endometriosis</subject><subject>Endometriosis - genetics</subject><subject>Endometriosis - physiopathology</subject><subject>Endothelial Growth Factors - genetics</subject><subject>Endothelial Growth Factors - metabolism</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Fertilization in Vitro</subject><subject>Flow Cytometry</subject><subject>follicular environment</subject><subject>granulosa cells</subject><subject>Granulosa Cells - physiology</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>interleukin-6 (IL-6)</subject><subject>Interleukin-6 - genetics</subject><subject>Interleukin-6 - metabolism</subject><subject>Lymphokines - genetics</subject><subject>Lymphokines - metabolism</subject><subject>Medical sciences</subject><subject>Non tumoral diseases</subject><subject>oocyte quality</subject><subject>Polymerase Chain Reaction</subject><subject>Reference Values</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Sperm Injections, Intracytoplasmic</subject><subject>Transcription, Genetic</subject><subject>vascular endothelial growth factor (VEGF)</subject><subject>Vascular Endothelial Growth Factor A</subject><subject>Vascular Endothelial Growth Factors</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1TAQhS0EopfCI4C8AYFEwI5jJ1lVqCo_UiUWwNpy7Ak1OPHF47T0hXhOnNwrumRjz0jfmRmdQ8hTzt5wxtXbL4xxWbG6q18y_orxXvFK3SM7LqWqpJLiPtn9Q07II8QfjDHF2_ohOeFcirrpxI78ufi9T4Do4_ya7lN0i81bbWZHEWyCtaVxpNcG7RJMojC7mK8geBPo9xRv8hUdjc0xbRo_Z0gBlp9-rhQdbgti5iVENNRCCEg9UhunvUlmCFBwehMnKK8vc9YBaxGXvK2ZICcf0eNj8mA0AeHJ8T8l395ffD3_WF1-_vDp_N1lZRvR5aphcpR8sJYJ45TpO9bLdpDKGTW6tq-Vc0IMxg61bbuOu4E1kjVqgH60TColTsmLw9xixa8FMOvJ43q3mSEuqFvORc_rFZQH0KaImGDU--Qnk241Z3oNSG8B6dV9zbjeAtKr7tlxwTJM4O5Ux0QK8PwIFL9NGIt71uMd15QMZc0Ld3bgoNhx7SFptB5mC84nsFm76P9zyl-7h7EA</recordid><startdate>20010901</startdate><enddate>20010901</enddate><creator>Garrido, Nicolás</creator><creator>Albert, Carmela</creator><creator>Krüssel, Jan S</creator><creator>O’Connor, José E</creator><creator>Remohı́, José</creator><creator>Simón, Carlos</creator><creator>Pellicer, Antonio</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010901</creationdate><title>Expression, production, and secretion of vascular endothelial growth factor and interleukin-6 by granulosa cells is comparable in women with and without endometriosis</title><author>Garrido, Nicolás ; Albert, Carmela ; Krüssel, Jan S ; O’Connor, José E ; Remohı́, José ; Simón, Carlos ; Pellicer, Antonio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-405f51bcc03ad6a980957b56da6fd7926dd33bacb2c7881db045046be9fc05663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Culture Media, Conditioned</topic><topic>DNA Primers</topic><topic>Embryo Transfer</topic><topic>Endometriosis</topic><topic>Endometriosis - genetics</topic><topic>Endometriosis - physiopathology</topic><topic>Endothelial Growth Factors - genetics</topic><topic>Endothelial Growth Factors - metabolism</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Fertilization in Vitro</topic><topic>Flow Cytometry</topic><topic>follicular environment</topic><topic>granulosa cells</topic><topic>Granulosa Cells - physiology</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>interleukin-6 (IL-6)</topic><topic>Interleukin-6 - genetics</topic><topic>Interleukin-6 - metabolism</topic><topic>Lymphokines - genetics</topic><topic>Lymphokines - metabolism</topic><topic>Medical sciences</topic><topic>Non tumoral diseases</topic><topic>oocyte quality</topic><topic>Polymerase Chain Reaction</topic><topic>Reference Values</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>Sperm Injections, Intracytoplasmic</topic><topic>Transcription, Genetic</topic><topic>vascular endothelial growth factor (VEGF)</topic><topic>Vascular Endothelial Growth Factor A</topic><topic>Vascular Endothelial Growth Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garrido, Nicolás</creatorcontrib><creatorcontrib>Albert, Carmela</creatorcontrib><creatorcontrib>Krüssel, Jan S</creatorcontrib><creatorcontrib>O’Connor, José E</creatorcontrib><creatorcontrib>Remohı́, José</creatorcontrib><creatorcontrib>Simón, Carlos</creatorcontrib><creatorcontrib>Pellicer, Antonio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garrido, Nicolás</au><au>Albert, Carmela</au><au>Krüssel, Jan S</au><au>O’Connor, José E</au><au>Remohı́, José</au><au>Simón, Carlos</au><au>Pellicer, Antonio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression, production, and secretion of vascular endothelial growth factor and interleukin-6 by granulosa cells is comparable in women with and without endometriosis</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2001-09-01</date><risdate>2001</risdate><volume>76</volume><issue>3</issue><spage>568</spage><epage>575</epage><pages>568-575</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>Objective: To investigate the production and secretion of interleukin (IL)-6 and vascular endothelial growth factor (VEGF) mRNA and protein by granulosa luteal cells (GCs) in vivo and in vitro in women with and without endometriosis.
Design: Prospective study.
Setting: A private, university-affiliated assisted reproduction unit and a university center.
Patient(s): Women with severe endometriosis (n = 6) or without the disease (n = 14) after laparoscopy, undergoing in vitro fertilization/intracytoplasmic sperm injection and embryo transfer.
Intervention(s): GCs were obtained from each aspirate.
Main Outcome Measure(s): Intracellular and secreted protein, as well as mRNA for both VEGF and IL-6 in GCs.
Result(s): The expression of VEGF and IL-6 mRNAs in vivo and in vitro was similar in both groups. Also, GCs from patients with endometriosis produced and secreted equal amounts of these proteins compared with controls without the disease, either in freshly isolated cells or in 24-hour cultures.
Conclusion(s): The GC function in terms of VEGF and IL-6 production does not seem to be altered in patients with endometriosis in comparison with those without this condition.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>11532483</pmid><doi>10.1016/S0015-0282(01)01961-6</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Fertility and sterility, 2001-09, Vol.76 (3), p.568-575 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Biological and medical sciences Cells, Cultured Culture Media, Conditioned DNA Primers Embryo Transfer Endometriosis Endometriosis - genetics Endometriosis - physiopathology Endothelial Growth Factors - genetics Endothelial Growth Factors - metabolism Enzyme-Linked Immunosorbent Assay Female Female genital diseases Fertilization in Vitro Flow Cytometry follicular environment granulosa cells Granulosa Cells - physiology Gynecology. Andrology. Obstetrics Humans interleukin-6 (IL-6) Interleukin-6 - genetics Interleukin-6 - metabolism Lymphokines - genetics Lymphokines - metabolism Medical sciences Non tumoral diseases oocyte quality Polymerase Chain Reaction Reference Values Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Sperm Injections, Intracytoplasmic Transcription, Genetic vascular endothelial growth factor (VEGF) Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors |
| title | Expression, production, and secretion of vascular endothelial growth factor and interleukin-6 by granulosa cells is comparable in women with and without endometriosis |
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