Single bead and hard tag decoding using accurate isotopic difference target analysis-encoded combinatorial libraries
A single fully automated generic microbore liquid chromatography electrospray time‐of‐flight protocol has been developed for the analysis of single beads, single beads with analytical constructs, and isotopically labelled dialkylamine hard tags. The protocol relies upon the incorporation of an isoto...
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| Veröffentlicht in: | Rapid communications in mass spectrometry 2000-05, Vol.14 (9), p.782-793 |
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| creator | Lane, Stephen J Pipe, Adrian |
| description | A single fully automated generic microbore liquid chromatography electrospray time‐of‐flight protocol has been developed for the analysis of single beads, single beads with analytical constructs, and isotopically labelled dialkylamine hard tags. The protocol relies upon the incorporation of an isotopic signature into the linker and/or derivatising molecule to give a specific isotopic ratio together with an accurate isotopic difference. These properties facilitate highly specific and sensitive analysis of beads and hard tags using accurate isotopic difference analysis (AIDA) without prior knowledge of the molecular weight. Three open access methods with automated processing have been developed around a core generic approach.
Copyright © 2000 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/(SICI)1097-0231(20000515)14:9<782::AID-RCM944>3.0.CO;2-D |
| format | Article |
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Copyright © 2000 John Wiley & Sons, Ltd.</description><subject>Amines - analysis</subject><subject>Automation</subject><subject>Carbon Isotopes</subject><subject>Chromatography, Liquid - methods</subject><subject>Combinatorial Chemistry Techniques</subject><subject>Dansyl Compounds - analysis</subject><subject>Fluorescence</subject><subject>Mass Spectrometry - methods</subject><subject>Polysaccharides - analysis</subject><issn>0951-4198</issn><issn>1097-0231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVtv00AQhS0EomnhLyA_ofbBYW9e2wGhFoeWSC1BbRGIl9FeJumCE4ddW5B_z0YOFRJI7MOuNHvmzOh8SXJKyZgSwl4c38zq2QklVZERxukxI_HkND-hYlK9Kko2mZzNptl1fVUJ8ZqPybiev2TZ9EEyum96mIxIldNM0Ko8SA5D-EoIpTkjj5MDSkqWE1qMku7GrZcNphqVTdXapnfK27RTy9SiaW38TPuwu5UxvVcdpi60XbtxJrVusUCPa4NR75fYxX7VbIMLWSy2Fm1q2pV2a9W13qkmbZz2yjsMT5JHC9UEfLp_j5KP529v63fZ5fxiVp9dZoYzITJdCiIV5yilZDIuqLlmWEpDCmZLw0hupbZWl6ZCq5XmBS-0XGgtaC6Y1PwoeT74bnz7vcfQwcoFg02j1tj2AQpKuSxlHoWfB6HxbQgeF7DxbqX8FiiBHRGAHRHYhQu7cOE3EaACKohEACIRGIgABwL1HBhMo_Wz_Q69XqH9w3hAEAVfBsEP1-D2r8H_nfvPsftKNM8Gcxc6_Hlvrvw3kDGtHD69v4Dy-sN5cSuu4A3_BWwLuJ4</recordid><startdate>20000515</startdate><enddate>20000515</enddate><creator>Lane, Stephen J</creator><creator>Pipe, Adrian</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000515</creationdate><title>Single bead and hard tag decoding using accurate isotopic difference target analysis-encoded combinatorial libraries</title><author>Lane, Stephen J ; Pipe, Adrian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3244-b8406a33e66626eadb3b2e86c072d8c205d6bddb8c9edbab3737b6fbb415426b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amines - analysis</topic><topic>Automation</topic><topic>Carbon Isotopes</topic><topic>Chromatography, Liquid - methods</topic><topic>Combinatorial Chemistry Techniques</topic><topic>Dansyl Compounds - analysis</topic><topic>Fluorescence</topic><topic>Mass Spectrometry - methods</topic><topic>Polysaccharides - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lane, Stephen J</creatorcontrib><creatorcontrib>Pipe, Adrian</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Rapid communications in mass spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lane, Stephen J</au><au>Pipe, Adrian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single bead and hard tag decoding using accurate isotopic difference target analysis-encoded combinatorial libraries</atitle><jtitle>Rapid communications in mass spectrometry</jtitle><addtitle>Rapid Commun. Mass Spectrom</addtitle><date>2000-05-15</date><risdate>2000</risdate><volume>14</volume><issue>9</issue><spage>782</spage><epage>793</epage><pages>782-793</pages><issn>0951-4198</issn><eissn>1097-0231</eissn><abstract>A single fully automated generic microbore liquid chromatography electrospray time‐of‐flight protocol has been developed for the analysis of single beads, single beads with analytical constructs, and isotopically labelled dialkylamine hard tags. The protocol relies upon the incorporation of an isotopic signature into the linker and/or derivatising molecule to give a specific isotopic ratio together with an accurate isotopic difference. These properties facilitate highly specific and sensitive analysis of beads and hard tags using accurate isotopic difference analysis (AIDA) without prior knowledge of the molecular weight. Three open access methods with automated processing have been developed around a core generic approach.
Copyright © 2000 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>10825017</pmid><doi>10.1002/(SICI)1097-0231(20000515)14:9<782::AID-RCM944>3.0.CO;2-D</doi><tpages>12</tpages></addata></record> |
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| identifier | ISSN: 0951-4198 |
| ispartof | Rapid communications in mass spectrometry, 2000-05, Vol.14 (9), p.782-793 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Amines - analysis Automation Carbon Isotopes Chromatography, Liquid - methods Combinatorial Chemistry Techniques Dansyl Compounds - analysis Fluorescence Mass Spectrometry - methods Polysaccharides - analysis |
| title | Single bead and hard tag decoding using accurate isotopic difference target analysis-encoded combinatorial libraries |
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