Removal of Inhibitory Substances with Recombinant Fibronectin-CH-296 Plates Enhances the Retroviral Transduction Efficiency of CD34+CD38− Bone Marrow Cells

In retroviral gene transduction, the efficiency of viral infection was reduced by the pro-teoglycans and some other materials secreted by the producer lines. In order to remove these inhibitors we have developed the rFN-CH-296-facilitated protocol. Because the rFN-CH-296 molecule has strong ability to bind a retroviral vector, rFN-CH-296 bound plates are utilized to wash out the unbound putative inhibitors present in a virus supernatant. The gene transduction efficiencies of human CD34+CD38− BMCs with a GALV-pseudotyped vector and the rFN-CH-296-facilitated protocol were compared with the protocol without a coating plate with CH-296, the mean gene transduction efficiencies being found to be 95.5 and 71.1%, respectively.