Evaluation of the potentiometric detection of acetylcholine and other neurotransmitters in capillary electrophoresis

It is demonstrated that acetylcholine may be determined potentiometrically in capillary electrophoresis down to 6 × 10—7 M with a miniature coated‐wire ion selective electrode. The more established amperometric method is not possible for this compound. Similarly, 4‐aminobutyric acid (GABA) is not ac...

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Veröffentlicht in:	Electrophoresis 2000-04, Vol.21 (7), p.1390-1394
Hauptverfasser:	Kappes, Thomas, Schnierle, Peter, Hauser, Peter C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylcholine Acetylcholine - analysis Amperometric detection Capillary electrophoresis Dopamine - analysis Electrophoresis, Capillary - methods Epinephrine - analysis Evaluation Studies as Topic gamma-Aminobutyric Acid - analysis Neurotransmitter Agents - analysis Neurotransmitters Norepinephrine - analysis Potentiometric detection Potentiometry - methods Serotonin - analysis
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creator	Kappes, Thomas Schnierle, Peter Hauser, Peter C.
description	It is demonstrated that acetylcholine may be determined potentiometrically in capillary electrophoresis down to 6 × 10—7 M with a miniature coated‐wire ion selective electrode. The more established amperometric method is not possible for this compound. Similarly, 4‐aminobutyric acid (GABA) is not accessible amperometrically; although potentiometric detection was possible, the sensitivity is not good. Other neurotransmitters may be determined by either method, but in these cases amperometric determination is generally favorable. For serotonin the potentiometric detection limit was 4 × 10—5 M, while for the amperometric method a detection limit of 10—7 M was found.
doi_str_mv	10.1002/(SICI)1522-2683(20000401)21:7<1390::AID-ELPS1390>3.0.CO;2-5
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The more established amperometric method is not possible for this compound. Similarly, 4‐aminobutyric acid (GABA) is not accessible amperometrically; although potentiometric detection was possible, the sensitivity is not good. Other neurotransmitters may be determined by either method, but in these cases amperometric determination is generally favorable. 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For serotonin the potentiometric detection limit was 4 × 10—5 M, while for the amperometric method a detection limit of 10—7 M was found.</description><subject>Acetylcholine</subject><subject>Acetylcholine - analysis</subject><subject>Amperometric detection</subject><subject>Capillary electrophoresis</subject><subject>Dopamine - analysis</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Epinephrine - analysis</subject><subject>Evaluation Studies as Topic</subject><subject>gamma-Aminobutyric Acid - analysis</subject><subject>Neurotransmitter Agents - analysis</subject><subject>Neurotransmitters</subject><subject>Norepinephrine - analysis</subject><subject>Potentiometric detection</subject><subject>Potentiometry - methods</subject><subject>Serotonin - analysis</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-P0zAQxSMEYsvCV0A5od1Div8ksVsQ0hLKUlEooiAOHEaOM1G9pHGxXaDfHkfdVkgI4Yvl8Zv3RvNLkoqSMSWEPb1Yzav5JS0Yy1gp-QUj8eSEXjI6Fc8pn5Dp9Gr-KpstPqyG1ws-JuNq-YxlxZ1kdOq7m4wIFTwjkhdnyQPvbwabSZ7fT84okawsZTFKwuyH6nYqGNuntk3DGtOtDdjHwgaDMzptMKA-_iuNYd_pte1Mj6nqm9TGFpf2uHM2ONX7jQkBnU9Nn2q1NV2n3D7FLlo4u11bh974h8m9VnUeH93e58nn17NP1ZtssbyeV1eLTOeSk6xuVanLpkRVt1KommrZTHhd66YkhSZlWTSyEEhlLnJsuZjIFie1UlQTUuct5-fJk4Pv1tnvO_QBNsZrjDP1aHceBKWcU5pH4deDUDvrvcMWts5s4uRACQxQAAYoMCwXhuXCEQowCgIGDAARChyhAAcC1RIYFNH98e0Yu3qDzR_eBwpRAAfBT9Ph_q_s_0f_I_lUiwnZIcH4gL9OCcp9g1JwUcCX99fA3r57KVZcwkf-GyRivM8</recordid><startdate>20000401</startdate><enddate>20000401</enddate><creator>Kappes, Thomas</creator><creator>Schnierle, Peter</creator><creator>Hauser, Peter C.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000401</creationdate><title>Evaluation of the potentiometric detection of acetylcholine and other neurotransmitters in capillary electrophoresis</title><author>Kappes, Thomas ; Schnierle, Peter ; Hauser, Peter C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4830-bfa6c6d6eabf87ab1c8d93bbcd605c0665d857e18474ef3798fe9baa1c00b4f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Acetylcholine</topic><topic>Acetylcholine - analysis</topic><topic>Amperometric detection</topic><topic>Capillary electrophoresis</topic><topic>Dopamine - analysis</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Epinephrine - analysis</topic><topic>Evaluation Studies as Topic</topic><topic>gamma-Aminobutyric Acid - analysis</topic><topic>Neurotransmitter Agents - analysis</topic><topic>Neurotransmitters</topic><topic>Norepinephrine - analysis</topic><topic>Potentiometric detection</topic><topic>Potentiometry - methods</topic><topic>Serotonin - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kappes, Thomas</creatorcontrib><creatorcontrib>Schnierle, Peter</creatorcontrib><creatorcontrib>Hauser, Peter C.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kappes, Thomas</au><au>Schnierle, Peter</au><au>Hauser, Peter C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the potentiometric detection of acetylcholine and other neurotransmitters in capillary electrophoresis</atitle><jtitle>Electrophoresis</jtitle><addtitle>ELECTROPHORESIS</addtitle><date>2000-04-01</date><risdate>2000</risdate><volume>21</volume><issue>7</issue><spage>1390</spage><epage>1394</epage><pages>1390-1394</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>It is demonstrated that acetylcholine may be determined potentiometrically in capillary electrophoresis down to 6 × 10—7 M with a miniature coated‐wire ion selective electrode. 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subjects	Acetylcholine Acetylcholine - analysis Amperometric detection Capillary electrophoresis Dopamine - analysis Electrophoresis, Capillary - methods Epinephrine - analysis Evaluation Studies as Topic gamma-Aminobutyric Acid - analysis Neurotransmitter Agents - analysis Neurotransmitters Norepinephrine - analysis Potentiometric detection Potentiometry - methods Serotonin - analysis
title	Evaluation of the potentiometric detection of acetylcholine and other neurotransmitters in capillary electrophoresis
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