MOZ is fused to p300 in an acute monocytic leukemia with t(8;22)
We report on the fusion of the monocytic leukemia zinc finger protein (MOZ) gene to the adenoviral E1A‐associated protein p300 (p300) gene in acute monocytic leukemia M5 associated with a t(8;22)(p11;q13) translocation. We studied two patients with double‐color fluorescence in situ hybridization (FI...
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| Veröffentlicht in: | Genes chromosomes & cancer 2000-06, Vol.28 (2), p.138-144 |
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| creator | Chaffanet, Max Gressin, Laetitia Preudhomme, Claude Soenen-Cornu, Valérie Birnbaum, Daniel Pébusque, Marie-Josèphe |
| description | We report on the fusion of the monocytic leukemia zinc finger protein (MOZ) gene to the adenoviral E1A‐associated protein p300 (p300) gene in acute monocytic leukemia M5 associated with a t(8;22)(p11;q13) translocation. We studied two patients with double‐color fluorescence in situ hybridization (FISH) using the yeast artificial chromosome 176C9 and the bacterial artificial chromosome clone H59D10 specific to the MOZ and p300 genes, respectively. Both probes were split in the patients' chromosome metaphase cells, and the two derivative chromosomes were each labeled with both probes. We showed by Southern blot the rearrangement of the MOZ gene, and cloned the fusion transcripts in one patient carrying the t(8;22) by reverse transcription‐polymerase chain reaction using MOZ‐ and p300‐specific primers. Both fusion transcripts were expressed. This result defines a novel reciprocal translocation involving two acetyltransferases, MOZ and p300, resulting in an abnormal transcriptional co‐activator that could play a critical role in leukemogenesis. Genes Chromosomes Cancer 28:138–144, 2000. © 2000 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/(SICI)1098-2264(200006)28:2<138::AID-GCC2>3.0.CO;2-2 |
| format | Article |
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We studied two patients with double‐color fluorescence in situ hybridization (FISH) using the yeast artificial chromosome 176C9 and the bacterial artificial chromosome clone H59D10 specific to the MOZ and p300 genes, respectively. Both probes were split in the patients' chromosome metaphase cells, and the two derivative chromosomes were each labeled with both probes. We showed by Southern blot the rearrangement of the MOZ gene, and cloned the fusion transcripts in one patient carrying the t(8;22) by reverse transcription‐polymerase chain reaction using MOZ‐ and p300‐specific primers. Both fusion transcripts were expressed. This result defines a novel reciprocal translocation involving two acetyltransferases, MOZ and p300, resulting in an abnormal transcriptional co‐activator that could play a critical role in leukemogenesis. Genes Chromosomes Cancer 28:138–144, 2000. © 2000 Wiley‐Liss, Inc.</description><identifier>ISSN: 1045-2257</identifier><identifier>EISSN: 1098-2264</identifier><identifier>DOI: 10.1002/(SICI)1098-2264(200006)28:2<138::AID-GCC2>3.0.CO;2-2</identifier><identifier>PMID: 10824998</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Acetyltransferases - genetics ; Acetyltransferases - isolation & purification ; Amino Acid Sequence ; Chromosomes, Human, Pair 22 - genetics ; Chromosomes, Human, Pair 8 - genetics ; E1A-Associated p300 Protein ; Gene Rearrangement ; Histone Acetyltransferases ; Humans ; In Situ Hybridization, Fluorescence ; Leukemia, Monocytic, Acute - enzymology ; Leukemia, Monocytic, Acute - genetics ; Leukemia, Myelomonocytic, Chronic - enzymology ; Leukemia, Myelomonocytic, Chronic - genetics ; Molecular Sequence Data ; Nuclear Proteins - genetics ; Nuclear Proteins - isolation & purification ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - isolation & purification ; RNA, Messenger - isolation & purification ; Trans-Activators - genetics ; Trans-Activators - isolation & purification ; Translocation, Genetic - genetics ; Tumor Cells, Cultured</subject><ispartof>Genes chromosomes & cancer, 2000-06, Vol.28 (2), p.138-144</ispartof><rights>Copyright © 2000 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291098-2264%28200006%2928%3A2%3C138%3A%3AAID-GCC2%3E3.0.CO%3B2-2$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291098-2264%28200006%2928%3A2%3C138%3A%3AAID-GCC2%3E3.0.CO%3B2-2$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10824998$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chaffanet, Max</creatorcontrib><creatorcontrib>Gressin, Laetitia</creatorcontrib><creatorcontrib>Preudhomme, Claude</creatorcontrib><creatorcontrib>Soenen-Cornu, Valérie</creatorcontrib><creatorcontrib>Birnbaum, Daniel</creatorcontrib><creatorcontrib>Pébusque, Marie-Josèphe</creatorcontrib><title>MOZ is fused to p300 in an acute monocytic leukemia with t(8;22)</title><title>Genes chromosomes & cancer</title><addtitle>Genes Chromosom. Cancer</addtitle><description>We report on the fusion of the monocytic leukemia zinc finger protein (MOZ) gene to the adenoviral E1A‐associated protein p300 (p300) gene in acute monocytic leukemia M5 associated with a t(8;22)(p11;q13) translocation. We studied two patients with double‐color fluorescence in situ hybridization (FISH) using the yeast artificial chromosome 176C9 and the bacterial artificial chromosome clone H59D10 specific to the MOZ and p300 genes, respectively. Both probes were split in the patients' chromosome metaphase cells, and the two derivative chromosomes were each labeled with both probes. We showed by Southern blot the rearrangement of the MOZ gene, and cloned the fusion transcripts in one patient carrying the t(8;22) by reverse transcription‐polymerase chain reaction using MOZ‐ and p300‐specific primers. Both fusion transcripts were expressed. This result defines a novel reciprocal translocation involving two acetyltransferases, MOZ and p300, resulting in an abnormal transcriptional co‐activator that could play a critical role in leukemogenesis. Genes Chromosomes Cancer 28:138–144, 2000. © 2000 Wiley‐Liss, Inc.</description><subject>Acetyltransferases - genetics</subject><subject>Acetyltransferases - isolation & purification</subject><subject>Amino Acid Sequence</subject><subject>Chromosomes, Human, Pair 22 - genetics</subject><subject>Chromosomes, Human, Pair 8 - genetics</subject><subject>E1A-Associated p300 Protein</subject><subject>Gene Rearrangement</subject><subject>Histone Acetyltransferases</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Leukemia, Monocytic, Acute - enzymology</subject><subject>Leukemia, Monocytic, Acute - genetics</subject><subject>Leukemia, Myelomonocytic, Chronic - enzymology</subject><subject>Leukemia, Myelomonocytic, Chronic - genetics</subject><subject>Molecular Sequence Data</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - isolation & purification</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - isolation & purification</subject><subject>RNA, Messenger - isolation & purification</subject><subject>Trans-Activators - genetics</subject><subject>Trans-Activators - isolation & purification</subject><subject>Translocation, Genetic - genetics</subject><subject>Tumor Cells, Cultured</subject><issn>1045-2257</issn><issn>1098-2264</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkG9v0zAQxi0EYmPwFZBfofZFin3nJE43TUwZdIVCkRgM8ebkJo7mLWlKnWj028-hY8I6yffnuUe6H2MnUkykEPB29G2ez8dSZDoCSNQIRHjJGPQUTiTq6fRsfh7N8hxOcSIm-fIYInjCDh8Xng65ikMepwfshfc3wz5m8XN2IIUGlWX6kL37vPzFnedV723Ju5ZvUAju1tyEKPrO8qZdt8WucwWvbX9rG2f4neuueTfSxwDjl-xZZWpvXz38R-z7h_eX-UW0WM7m-dkicggAUSlFpbASiVGIaEBpCWW1AsjizKzKRBdprFSSWlGGDgpMlLRYGp2aVWVLhUfszd53s21_99Z31Dhf2Lo2a9v2nlIpEbJEBuHrB2G_amxJm61rzHZH_24Ogsu94M7VdvffnAbuNGCngSINFGmPnSCUFLBToE4DdUISlC9DF_7WwTba2zrf2T-PtmZ7S0mKaUxXX2aU__x49fX8xyda4D3i5YTV</recordid><startdate>200006</startdate><enddate>200006</enddate><creator>Chaffanet, Max</creator><creator>Gressin, Laetitia</creator><creator>Preudhomme, Claude</creator><creator>Soenen-Cornu, Valérie</creator><creator>Birnbaum, Daniel</creator><creator>Pébusque, Marie-Josèphe</creator><general>John Wiley & Sons, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200006</creationdate><title>MOZ is fused to p300 in an acute monocytic leukemia with t(8;22)</title><author>Chaffanet, Max ; Gressin, Laetitia ; Preudhomme, Claude ; Soenen-Cornu, Valérie ; Birnbaum, Daniel ; Pébusque, Marie-Josèphe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i3222-d10f43f06a4333a24812dfb22959abd68c754467e0d959303641e3da87abfed43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Acetyltransferases - genetics</topic><topic>Acetyltransferases - isolation & purification</topic><topic>Amino Acid Sequence</topic><topic>Chromosomes, Human, Pair 22 - genetics</topic><topic>Chromosomes, Human, Pair 8 - genetics</topic><topic>E1A-Associated p300 Protein</topic><topic>Gene Rearrangement</topic><topic>Histone Acetyltransferases</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Leukemia, Monocytic, Acute - enzymology</topic><topic>Leukemia, Monocytic, Acute - genetics</topic><topic>Leukemia, Myelomonocytic, Chronic - enzymology</topic><topic>Leukemia, Myelomonocytic, Chronic - genetics</topic><topic>Molecular Sequence Data</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - isolation & purification</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - isolation & purification</topic><topic>RNA, Messenger - isolation & purification</topic><topic>Trans-Activators - genetics</topic><topic>Trans-Activators - isolation & purification</topic><topic>Translocation, Genetic - genetics</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chaffanet, Max</creatorcontrib><creatorcontrib>Gressin, Laetitia</creatorcontrib><creatorcontrib>Preudhomme, Claude</creatorcontrib><creatorcontrib>Soenen-Cornu, Valérie</creatorcontrib><creatorcontrib>Birnbaum, Daniel</creatorcontrib><creatorcontrib>Pébusque, Marie-Josèphe</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Genes chromosomes & cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chaffanet, Max</au><au>Gressin, Laetitia</au><au>Preudhomme, Claude</au><au>Soenen-Cornu, Valérie</au><au>Birnbaum, Daniel</au><au>Pébusque, Marie-Josèphe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MOZ is fused to p300 in an acute monocytic leukemia with t(8;22)</atitle><jtitle>Genes chromosomes & cancer</jtitle><addtitle>Genes Chromosom. Cancer</addtitle><date>2000-06</date><risdate>2000</risdate><volume>28</volume><issue>2</issue><spage>138</spage><epage>144</epage><pages>138-144</pages><issn>1045-2257</issn><eissn>1098-2264</eissn><abstract>We report on the fusion of the monocytic leukemia zinc finger protein (MOZ) gene to the adenoviral E1A‐associated protein p300 (p300) gene in acute monocytic leukemia M5 associated with a t(8;22)(p11;q13) translocation. We studied two patients with double‐color fluorescence in situ hybridization (FISH) using the yeast artificial chromosome 176C9 and the bacterial artificial chromosome clone H59D10 specific to the MOZ and p300 genes, respectively. Both probes were split in the patients' chromosome metaphase cells, and the two derivative chromosomes were each labeled with both probes. We showed by Southern blot the rearrangement of the MOZ gene, and cloned the fusion transcripts in one patient carrying the t(8;22) by reverse transcription‐polymerase chain reaction using MOZ‐ and p300‐specific primers. Both fusion transcripts were expressed. This result defines a novel reciprocal translocation involving two acetyltransferases, MOZ and p300, resulting in an abnormal transcriptional co‐activator that could play a critical role in leukemogenesis. Genes Chromosomes Cancer 28:138–144, 2000. © 2000 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>10824998</pmid><doi>10.1002/(SICI)1098-2264(200006)28:2<138::AID-GCC2>3.0.CO;2-2</doi><tpages>7</tpages></addata></record> |
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| subjects | Acetyltransferases - genetics Acetyltransferases - isolation & purification Amino Acid Sequence Chromosomes, Human, Pair 22 - genetics Chromosomes, Human, Pair 8 - genetics E1A-Associated p300 Protein Gene Rearrangement Histone Acetyltransferases Humans In Situ Hybridization, Fluorescence Leukemia, Monocytic, Acute - enzymology Leukemia, Monocytic, Acute - genetics Leukemia, Myelomonocytic, Chronic - enzymology Leukemia, Myelomonocytic, Chronic - genetics Molecular Sequence Data Nuclear Proteins - genetics Nuclear Proteins - isolation & purification Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - isolation & purification RNA, Messenger - isolation & purification Trans-Activators - genetics Trans-Activators - isolation & purification Translocation, Genetic - genetics Tumor Cells, Cultured |
| title | MOZ is fused to p300 in an acute monocytic leukemia with t(8;22) |
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