Detection of Individual Differences in Radiation-Induced Apoptosis of Peripheral Blood Lymphocytes in Normal Individuals, Ataxia Telangiectasia Homozygotes and Heterozygotes, and Breast Cancer Patients after Radiotherapy

Barber, J. B. P., West, C. M. L., Kiltie, A. E., Roberts, S. A. and Scott, D. Detection of Individual Differences in Radiation-Induced Apoptosis of Peripheral Blood Lymphocytes in Normal Individuals, Ataxia Telangiectasia Homozygotes and Heterozygotes, and Breast Cancer Patients after Radiotherapy....

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Veröffentlicht in:Radiation research 2000-05, Vol.153 (5), p.570-578
Hauptverfasser: Barber, J. B P., West, C. M L., Kiltie, A. E., Roberts, S. A., Scott, D.
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container_end_page 578
container_issue 5
container_start_page 570
container_title Radiation research
container_volume 153
creator Barber, J. B P.
West, C. M L.
Kiltie, A. E.
Roberts, S. A.
Scott, D.
description Barber, J. B. P., West, C. M. L., Kiltie, A. E., Roberts, S. A. and Scott, D. Detection of Individual Differences in Radiation-Induced Apoptosis of Peripheral Blood Lymphocytes in Normal Individuals, Ataxia Telangiectasia Homozygotes and Heterozygotes, and Breast Cancer Patients after Radiotherapy. Quantification of radiation-induced apoptosis in peripheral blood lymphocytes (PBLs) has been proposed as a possible screening test for cancer-prone individuals and also for the prediction of normal tissue responses after radiotherapy. We have used the TUNEL assay (terminal transferase nick-end labeling) 24 h after irradiation with 4 Gy at high dose rate to assess interindividual differences in radiation-induced apoptosis between (1) a panel of normal individuals, (2) ataxia telangiectasia (AT) homozygotes and heterozygotes, and (3) breast cancer patients who had received radiotherapy 8–13 years ago, including a number of patients who had suffered adverse responses to radiation. With this protocol, we show clear differences in radiation-induced apoptosis between individuals, and good reproducibility in the assay. In agreement with previous reports using EBV-transformed lymphoblasts, we show a very poor induction of apoptosis in AT homozygotes and a reduced level in AT heterozygotes compared to normal individuals. A similar reduced level compared to normal individuals was seen in the breast cancer patients. Despite a wide range of values in the breast cancer patients and good reproducibility on repeat samples, there was no correlation of rates of apoptosis with the severity of breast fibrosis, retraction or telangiectasia. The reduced rate of apoptosis observed in the breast cancer cases may be associated with genetic predisposition to breast cancer; however, we conclude that assays of lymphocyte apoptosis are unlikely to be of use in predicting normal tissue tolerance to radiotherapy.
doi_str_mv 10.1667/0033-7587(2000)153[0570:DOIDIR]2.0.CO;2
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We have used the TUNEL assay (terminal transferase nick-end labeling) 24 h after irradiation with 4 Gy at high dose rate to assess interindividual differences in radiation-induced apoptosis between (1) a panel of normal individuals, (2) ataxia telangiectasia (AT) homozygotes and heterozygotes, and (3) breast cancer patients who had received radiotherapy 8–13 years ago, including a number of patients who had suffered adverse responses to radiation. With this protocol, we show clear differences in radiation-induced apoptosis between individuals, and good reproducibility in the assay. In agreement with previous reports using EBV-transformed lymphoblasts, we show a very poor induction of apoptosis in AT homozygotes and a reduced level in AT heterozygotes compared to normal individuals. A similar reduced level compared to normal individuals was seen in the breast cancer patients. Despite a wide range of values in the breast cancer patients and good reproducibility on repeat samples, there was no correlation of rates of apoptosis with the severity of breast fibrosis, retraction or telangiectasia. 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B P.</creatorcontrib><creatorcontrib>West, C. M L.</creatorcontrib><creatorcontrib>Kiltie, A. E.</creatorcontrib><creatorcontrib>Roberts, S. A.</creatorcontrib><creatorcontrib>Scott, D.</creatorcontrib><title>Detection of Individual Differences in Radiation-Induced Apoptosis of Peripheral Blood Lymphocytes in Normal Individuals, Ataxia Telangiectasia Homozygotes and Heterozygotes, and Breast Cancer Patients after Radiotherapy</title><title>Radiation research</title><addtitle>Radiat Res</addtitle><description>Barber, J. B. P., West, C. M. L., Kiltie, A. E., Roberts, S. A. and Scott, D. Detection of Individual Differences in Radiation-Induced Apoptosis of Peripheral Blood Lymphocytes in Normal Individuals, Ataxia Telangiectasia Homozygotes and Heterozygotes, and Breast Cancer Patients after Radiotherapy. Quantification of radiation-induced apoptosis in peripheral blood lymphocytes (PBLs) has been proposed as a possible screening test for cancer-prone individuals and also for the prediction of normal tissue responses after radiotherapy. We have used the TUNEL assay (terminal transferase nick-end labeling) 24 h after irradiation with 4 Gy at high dose rate to assess interindividual differences in radiation-induced apoptosis between (1) a panel of normal individuals, (2) ataxia telangiectasia (AT) homozygotes and heterozygotes, and (3) breast cancer patients who had received radiotherapy 8–13 years ago, including a number of patients who had suffered adverse responses to radiation. With this protocol, we show clear differences in radiation-induced apoptosis between individuals, and good reproducibility in the assay. In agreement with previous reports using EBV-transformed lymphoblasts, we show a very poor induction of apoptosis in AT homozygotes and a reduced level in AT heterozygotes compared to normal individuals. A similar reduced level compared to normal individuals was seen in the breast cancer patients. Despite a wide range of values in the breast cancer patients and good reproducibility on repeat samples, there was no correlation of rates of apoptosis with the severity of breast fibrosis, retraction or telangiectasia. 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A.</au><au>Scott, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Individual Differences in Radiation-Induced Apoptosis of Peripheral Blood Lymphocytes in Normal Individuals, Ataxia Telangiectasia Homozygotes and Heterozygotes, and Breast Cancer Patients after Radiotherapy</atitle><jtitle>Radiation research</jtitle><addtitle>Radiat Res</addtitle><date>2000-05-01</date><risdate>2000</risdate><volume>153</volume><issue>5</issue><spage>570</spage><epage>578</epage><pages>570-578</pages><issn>0033-7587</issn><eissn>1938-5404</eissn><coden>RAREAE</coden><abstract>Barber, J. B. P., West, C. M. L., Kiltie, A. E., Roberts, S. A. and Scott, D. Detection of Individual Differences in Radiation-Induced Apoptosis of Peripheral Blood Lymphocytes in Normal Individuals, Ataxia Telangiectasia Homozygotes and Heterozygotes, and Breast Cancer Patients after Radiotherapy. Quantification of radiation-induced apoptosis in peripheral blood lymphocytes (PBLs) has been proposed as a possible screening test for cancer-prone individuals and also for the prediction of normal tissue responses after radiotherapy. We have used the TUNEL assay (terminal transferase nick-end labeling) 24 h after irradiation with 4 Gy at high dose rate to assess interindividual differences in radiation-induced apoptosis between (1) a panel of normal individuals, (2) ataxia telangiectasia (AT) homozygotes and heterozygotes, and (3) breast cancer patients who had received radiotherapy 8–13 years ago, including a number of patients who had suffered adverse responses to radiation. With this protocol, we show clear differences in radiation-induced apoptosis between individuals, and good reproducibility in the assay. In agreement with previous reports using EBV-transformed lymphoblasts, we show a very poor induction of apoptosis in AT homozygotes and a reduced level in AT heterozygotes compared to normal individuals. A similar reduced level compared to normal individuals was seen in the breast cancer patients. Despite a wide range of values in the breast cancer patients and good reproducibility on repeat samples, there was no correlation of rates of apoptosis with the severity of breast fibrosis, retraction or telangiectasia. The reduced rate of apoptosis observed in the breast cancer cases may be associated with genetic predisposition to breast cancer; however, we conclude that assays of lymphocyte apoptosis are unlikely to be of use in predicting normal tissue tolerance to radiotherapy.</abstract><cop>Oak Brook, Il</cop><pub>Radiation Research Society</pub><pmid>10790278</pmid><doi>10.1667/0033-7587(2000)153[0570:DOIDIR]2.0.CO;2</doi><tpages>9</tpages></addata></record>
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identifier ISSN: 0033-7587
ispartof Radiation research, 2000-05, Vol.153 (5), p.570-578
issn 0033-7587
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source MEDLINE; BioOne Complete; JSTOR Archive Collection A-Z Listing
subjects Adult
Apoptosis
Apoptosis - radiation effects
Ataxia telangiectasia
Ataxia Telangiectasia - blood
Ataxia Telangiectasia - genetics
Biological and medical sciences
Blood
Breast cancer
Breast Neoplasms - blood
Breast Neoplasms - radiotherapy
Case-Control Studies
Cohort Studies
Cytometry
Female
Flow Cytometry
Heterozygote
Heterozygotes
Homozygote
Homozygotes
Humans
In Situ Nick-End Labeling
Light
Lymphocytes
Lymphocytes - radiation effects
Male
Medical sciences
Middle Aged
Radiation tolerance
Radiotherapy
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
REGULAR ARTICLES
Reproducibility of Results
Scattering, Radiation
Space life sciences
Technology. Biomaterials. Equipments. Material. Instrumentation
title Detection of Individual Differences in Radiation-Induced Apoptosis of Peripheral Blood Lymphocytes in Normal Individuals, Ataxia Telangiectasia Homozygotes and Heterozygotes, and Breast Cancer Patients after Radiotherapy
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