Detection of Active Tuberculosis Infection by T Cell Responses to Early-Secreted Antigenic Target 6-kDa Protein and Culture Filtrate Protein 10
The purified protein derivative (PPD) skin test has no predictive value for tuberculosis (TB) in Mycobacterium bovis bacillus Calmette-Guérin (BCG)—vaccinated individuals because of cross-reactive responses to nonspecific constituents of PPD. T cell responses to early-secreted antigenic target 6-kDa...
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| Veröffentlicht in: | The Journal of infectious diseases 2000-05, Vol.181 (5), p.1850-1854 |
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| container_title | The Journal of infectious diseases |
| container_volume | 181 |
| creator | Arend, Sandra M. Andersen, Peter van Meijgaarden, Krista E. Skjøt, Rikke L. V. Subronto, Yanri W. van Dissel, Jaap T. Ottenhoff, Tom H. M. |
| description | The purified protein derivative (PPD) skin test has no predictive value for tuberculosis (TB) in Mycobacterium bovis bacillus Calmette-Guérin (BCG)—vaccinated individuals because of cross-reactive responses to nonspecific constituents of PPD. T cell responses to early-secreted antigenic target 6-kDa protein (ESAT-6) and the newly identified culture filtrate protein 10 (CFP-10), 2 proteins specifically expressed by M. tuberculosis (MTB) but not by BCG strains, were evaluated. Most TB patients responded to ESAT-6 (92%) or CFP-10 (89%). A minority of BCG-vaccinated individuals responded to both ESAT-6 and CFP-10, their history being consistent with latent infection with MTB in the presence of protective immunity. No responses were found in PPD-negative controls. The sensitivity and specificity of the assay were 84% and 100%, respectively, at a cutoff of 300 pg of interferon-γ/mL. These data indicate that ESAT-6 and CFP-10 are promising antigens for highly specific immunodiagnosis of TB, even in BCG-vaccinated individuals. |
| doi_str_mv | 10.1086/315448 |
| format | Article |
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No responses were found in PPD-negative controls. The sensitivity and specificity of the assay were 84% and 100%, respectively, at a cutoff of 300 pg of interferon-γ/mL. 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V.</creatorcontrib><creatorcontrib>Subronto, Yanri W.</creatorcontrib><creatorcontrib>van Dissel, Jaap T.</creatorcontrib><creatorcontrib>Ottenhoff, Tom H. M.</creatorcontrib><title>Detection of Active Tuberculosis Infection by T Cell Responses to Early-Secreted Antigenic Target 6-kDa Protein and Culture Filtrate Protein 10</title><title>The Journal of infectious diseases</title><addtitle>The Journal of Infectious Diseases</addtitle><description>The purified protein derivative (PPD) skin test has no predictive value for tuberculosis (TB) in Mycobacterium bovis bacillus Calmette-Guérin (BCG)—vaccinated individuals because of cross-reactive responses to nonspecific constituents of PPD. T cell responses to early-secreted antigenic target 6-kDa protein (ESAT-6) and the newly identified culture filtrate protein 10 (CFP-10), 2 proteins specifically expressed by M. tuberculosis (MTB) but not by BCG strains, were evaluated. Most TB patients responded to ESAT-6 (92%) or CFP-10 (89%). A minority of BCG-vaccinated individuals responded to both ESAT-6 and CFP-10, their history being consistent with latent infection with MTB in the presence of protective immunity. No responses were found in PPD-negative controls. The sensitivity and specificity of the assay were 84% and 100%, respectively, at a cutoff of 300 pg of interferon-γ/mL. These data indicate that ESAT-6 and CFP-10 are promising antigens for highly specific immunodiagnosis of TB, even in BCG-vaccinated individuals.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Antibody Formation</subject><subject>Antigens</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacillus calmette guerin vaccine</subject><subject>Bacterial diseases</subject><subject>Bacterial Proteins - immunology</subject><subject>BCG Vaccine</subject><subject>Biological and medical sciences</subject><subject>CFP-10 protein</subject><subject>Child</subject><subject>Concise Communications</subject><subject>Cutaneous tuberculosis</subject><subject>ESAT-6 protein</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Latent tuberculosis</subject><subject>Lymphocyte Activation</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Mycobacterium tuberculosis</subject><subject>ROC Curve</subject><subject>Skin tests</subject><subject>T lymphocytes</subject><subject>T-Lymphocytes - immunology</subject><subject>Tuberculin</subject><subject>Tuberculin Test</subject><subject>Tuberculosis - immunology</subject><subject>Tuberculosis - prevention & control</subject><subject>Tuberculosis and atypical mycobacterial infections</subject><subject>Tuberculosis vaccine</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhQtRnHbUf6AEBXeleVTlsWx6njD4mGlB3IRU1c1QPdVJT5KS6V8xf9k01bTixkW4gfPdQ05OUbwm-CPBkn9ipK4q-aSYkZqJknPCnhYzjCktiVTqqHgR4wpjXDEunhdHeYUyifGseDyBBG3qvUPeonm-_QK0HBsI7Tj42Ed06eweaLZoiRYwDOga4sa7CBElj05NGLblDbQhW3Vo7lJ_C65v0dKEW0iIl3cnBn0NPkHvkHEdWoxDGgOgs35IwSQ4iAS_LJ5ZM0R4tZ_Hxfez0-Xiorz6cn65mF-VbUVJKjvVYJBQWUsIqLqrLGsE1MAwEYR2lSDWUjBCqK7ju1NxbizlohFKMmDsuPgw-W6Cvx8hJr3uY5uzGQd-jFoQQqXg6r8gEVIxKWQG3_0DrvwYXA6hKWUK57fgP25t8DEGsHoT-rUJW02w3hWppyIz-HbvNjZr6P7CpuYy8H4PmNiawQbj2j4eOIkrpXYx30zUKiYfDmr-JoJpTbJeTnofEzwcdBPuNBdM1Prix099zq8_L_HNN83Zb_ckvCg</recordid><startdate>20000501</startdate><enddate>20000501</enddate><creator>Arend, Sandra M.</creator><creator>Andersen, Peter</creator><creator>van Meijgaarden, Krista E.</creator><creator>Skjøt, Rikke L. V.</creator><creator>Subronto, Yanri W.</creator><creator>van Dissel, Jaap T.</creator><creator>Ottenhoff, Tom H. M.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20000501</creationdate><title>Detection of Active Tuberculosis Infection by T Cell Responses to Early-Secreted Antigenic Target 6-kDa Protein and Culture Filtrate Protein 10</title><author>Arend, Sandra M. ; Andersen, Peter ; van Meijgaarden, Krista E. ; Skjøt, Rikke L. V. ; Subronto, Yanri W. ; van Dissel, Jaap T. ; Ottenhoff, Tom H. 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M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arend, Sandra M.</au><au>Andersen, Peter</au><au>van Meijgaarden, Krista E.</au><au>Skjøt, Rikke L. V.</au><au>Subronto, Yanri W.</au><au>van Dissel, Jaap T.</au><au>Ottenhoff, Tom H. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Active Tuberculosis Infection by T Cell Responses to Early-Secreted Antigenic Target 6-kDa Protein and Culture Filtrate Protein 10</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>The Journal of Infectious Diseases</addtitle><date>2000-05-01</date><risdate>2000</risdate><volume>181</volume><issue>5</issue><spage>1850</spage><epage>1854</epage><pages>1850-1854</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>The purified protein derivative (PPD) skin test has no predictive value for tuberculosis (TB) in Mycobacterium bovis bacillus Calmette-Guérin (BCG)—vaccinated individuals because of cross-reactive responses to nonspecific constituents of PPD. T cell responses to early-secreted antigenic target 6-kDa protein (ESAT-6) and the newly identified culture filtrate protein 10 (CFP-10), 2 proteins specifically expressed by M. tuberculosis (MTB) but not by BCG strains, were evaluated. Most TB patients responded to ESAT-6 (92%) or CFP-10 (89%). A minority of BCG-vaccinated individuals responded to both ESAT-6 and CFP-10, their history being consistent with latent infection with MTB in the presence of protective immunity. No responses were found in PPD-negative controls. The sensitivity and specificity of the assay were 84% and 100%, respectively, at a cutoff of 300 pg of interferon-γ/mL. These data indicate that ESAT-6 and CFP-10 are promising antigens for highly specific immunodiagnosis of TB, even in BCG-vaccinated individuals.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>10823800</pmid><doi>10.1086/315448</doi><tpages>5</tpages></addata></record> |
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| subjects | Adolescent Adult Aged Antibody Formation Antigens Antigens, Bacterial - immunology Bacillus calmette guerin vaccine Bacterial diseases Bacterial Proteins - immunology BCG Vaccine Biological and medical sciences CFP-10 protein Child Concise Communications Cutaneous tuberculosis ESAT-6 protein Human bacterial diseases Humans Infections Infectious diseases Latent tuberculosis Lymphocyte Activation Medical sciences Middle Aged Mycobacterium tuberculosis ROC Curve Skin tests T lymphocytes T-Lymphocytes - immunology Tuberculin Tuberculin Test Tuberculosis - immunology Tuberculosis - prevention & control Tuberculosis and atypical mycobacterial infections Tuberculosis vaccine |
| title | Detection of Active Tuberculosis Infection by T Cell Responses to Early-Secreted Antigenic Target 6-kDa Protein and Culture Filtrate Protein 10 |
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