Macrocyclic Chelators with Paramagnetic Cations Are Internalized into Mammalian Cells via a HIV-Tat Derived Membrane Translocation Peptide
A major obstacle to using paramagnetic MR contrast agents for in vivo cell tracking or molecular sensing is their generally low cellular uptake. In this study, we show that a paramagnetically labeled DOTA chelator derivatized with a 13-mer HIV-tat peptide is efficiently internalized into mammalian c...
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| Veröffentlicht in: | Bioconjugate chemistry 2000-05, Vol.11 (3), p.301-305 |
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| container_end_page | 305 |
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| container_title | Bioconjugate chemistry |
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| creator | Bhorade, Rajeev Weissleder, Ralph Nakakoshi, Tsunenori Moore, Anna Tung, Ching-Hsuan |
| description | A major obstacle to using paramagnetic MR contrast agents for in vivo cell tracking or molecular sensing is their generally low cellular uptake. In this study, we show that a paramagnetically labeled DOTA chelator derivatized with a 13-mer HIV-tat peptide is efficiently internalized into mammalian cells. Intracellular concentrations were attained that were readily detectable by MR imaging using both gadolinium and dysprosium chelates. Using this paradigm, it should be feasible to internalize a variety of chemically different agents into mammalian cells. |
| doi_str_mv | 10.1021/bc990168d |
| format | Article |
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Weissleder, Ralph ; Nakakoshi, Tsunenori ; Moore, Anna ; Tung, Ching-Hsuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a349t-22382883135cdce4ebae0ac0ef9cda9cf7a53ec7989947747a66f7f55e682e3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>AIDS/HIV</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biological Transport</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Nucleus - metabolism</topic><topic>Chelating Agents - metabolism</topic><topic>Cytoplasm - metabolism</topic><topic>Dysprosium</topic><topic>Gadolinium</topic><topic>Gene Products, tat - chemical synthesis</topic><topic>Gene Products, tat - chemistry</topic><topic>Gene Products, tat - metabolism</topic><topic>HeLa Cells</topic><topic>Heterocyclic Compounds, 1-Ring - chemical synthesis</topic><topic>Heterocyclic Compounds, 1-Ring - chemistry</topic><topic>Heterocyclic Compounds, 1-Ring - metabolism</topic><topic>Humans</topic><topic>Indium Radioisotopes</topic><topic>Lymphocytes - metabolism</topic><topic>Lymphocytes - ultrastructure</topic><topic>Magnetic Resonance Imaging</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Peptide Fragments - chemistry</topic><topic>Peptide Fragments - metabolism</topic><topic>tat Gene Products, Human Immunodeficiency Virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bhorade, Rajeev</creatorcontrib><creatorcontrib>Weissleder, Ralph</creatorcontrib><creatorcontrib>Nakakoshi, Tsunenori</creatorcontrib><creatorcontrib>Moore, Anna</creatorcontrib><creatorcontrib>Tung, Ching-Hsuan</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioconjugate chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bhorade, Rajeev</au><au>Weissleder, Ralph</au><au>Nakakoshi, Tsunenori</au><au>Moore, Anna</au><au>Tung, Ching-Hsuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Macrocyclic Chelators with Paramagnetic Cations Are Internalized into Mammalian Cells via a HIV-Tat Derived Membrane Translocation Peptide</atitle><jtitle>Bioconjugate chemistry</jtitle><addtitle>Bioconjugate Chem</addtitle><date>2000-05-01</date><risdate>2000</risdate><volume>11</volume><issue>3</issue><spage>301</spage><epage>305</epage><pages>301-305</pages><issn>1043-1802</issn><eissn>1520-4812</eissn><abstract>A major obstacle to using paramagnetic MR contrast agents for in vivo cell tracking or molecular sensing is their generally low cellular uptake. In this study, we show that a paramagnetically labeled DOTA chelator derivatized with a 13-mer HIV-tat peptide is efficiently internalized into mammalian cells. Intracellular concentrations were attained that were readily detectable by MR imaging using both gadolinium and dysprosium chelates. Using this paradigm, it should be feasible to internalize a variety of chemically different agents into mammalian cells.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>10821645</pmid><doi>10.1021/bc990168d</doi><tpages>5</tpages></addata></record> |
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| ispartof | Bioconjugate chemistry, 2000-05, Vol.11 (3), p.301-305 |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | AIDS/HIV Amino Acid Sequence Animals Biological Transport Cell Membrane - metabolism Cell Nucleus - metabolism Chelating Agents - metabolism Cytoplasm - metabolism Dysprosium Gadolinium Gene Products, tat - chemical synthesis Gene Products, tat - chemistry Gene Products, tat - metabolism HeLa Cells Heterocyclic Compounds, 1-Ring - chemical synthesis Heterocyclic Compounds, 1-Ring - chemistry Heterocyclic Compounds, 1-Ring - metabolism Humans Indium Radioisotopes Lymphocytes - metabolism Lymphocytes - ultrastructure Magnetic Resonance Imaging Mice Molecular Sequence Data Peptide Fragments - chemistry Peptide Fragments - metabolism tat Gene Products, Human Immunodeficiency Virus |
| title | Macrocyclic Chelators with Paramagnetic Cations Are Internalized into Mammalian Cells via a HIV-Tat Derived Membrane Translocation Peptide |
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