Mechanisms of pH Regulation in the Regulated Secretory Pathway

A precise pH gradient between organelles of the regulated secretory pathway is required for sorting and processing of prohormones. We studied pH regulation in live endocrine cells by targeting biotin-based pH indicators to cellular organelles expressing avidin-chimera proteins. In AtT-20 cells, we f...

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Veröffentlicht in:	The Journal of biological chemistry 2001-08, Vol.276 (35), p.33027-33035
Hauptverfasser:	Wu, Minnie M., Grabe, Michael, Adams, Stephen, Tsien, Roger Y., Moore, Hsiao-Ping H., Machen, Terry E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology Chlorides - metabolism Endoplasmic Reticulum - physiology Golgi Apparatus - physiology Homeostasis Hydrogen-Ion Concentration Intracellular Membranes - drug effects Intracellular Membranes - physiology Kinetics Membrane Potentials - physiology Mice Models, Biological Models, Chemical Organelles - drug effects Organelles - physiology Pituitary Gland - physiology Pituitary Hormones - metabolism Protein Precursors - metabolism Proton-Translocating ATPases - metabolism Secretory Vesicles - physiology Tumor Cells, Cultured Vacuolar Proton-Translocating ATPases Valinomycin - pharmacology
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