Characterization and mapping of Rpi1, a late-blight resistance locus from diploid (1EBN) Mexican Solanum pinnatisectum
Solanum is a diverse genus with over 200 species occupying a range of habitats from the Southwestern United States to Central Chile. Germplasm evaluations have focused on species that can be crossed with S. tuberosum, while Mexican diploid (2n = 2x = 24) Solanum species with an Endosperm Balance Num...
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| Veröffentlicht in: | Molecular genetics and genomics : MGG 2001-08, Vol.265 (6), p.977-985 |
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| creator | Kuhl, J C Hanneman, Jr, R E Havey, M J |
| description | Solanum is a diverse genus with over 200 species occupying a range of habitats from the Southwestern United States to Central Chile. Germplasm evaluations have focused on species that can be crossed with S. tuberosum, while Mexican diploid (2n = 2x = 24) Solanum species with an Endosperm Balance Number (EBN) of 1 have received less attention because of poor crossability due to their ploidy and EBN. Recent changes in Phytophthora infestans populations have increased the need for new sources of genetic resistance to this fungus. We have characterized resistance to P. infestans in the Mexican 2x(1EBN) species S. pinnatisectum. An interspecific hybrid between resistant S. pinnatisectum and susceptible S. cardiophyllum plants was backcrossed to S. cardiophyllum to generate a family segregating for late-blight resistance. The diploid (1EBN) genetic map generated with 99 RFLP markers revealed extensive synteny with previously published potato maps. A single dominant late-blight resistance locus (Rpi1) from S. pinnatisectum was mapped to chromosome 7, a region previously not associated with late-blight resistance. Characterization of the P.infestans isolate used for disease evaluations revealed that it possessed the avirulence gene corresponding to the R9 resistance locus, indicating that Rpi1 could possibly correspond to R9. |
| doi_str_mv | 10.1007/s004380100490 |
| format | Article |
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Germplasm evaluations have focused on species that can be crossed with S. tuberosum, while Mexican diploid (2n = 2x = 24) Solanum species with an Endosperm Balance Number (EBN) of 1 have received less attention because of poor crossability due to their ploidy and EBN. Recent changes in Phytophthora infestans populations have increased the need for new sources of genetic resistance to this fungus. We have characterized resistance to P. infestans in the Mexican 2x(1EBN) species S. pinnatisectum. An interspecific hybrid between resistant S. pinnatisectum and susceptible S. cardiophyllum plants was backcrossed to S. cardiophyllum to generate a family segregating for late-blight resistance. The diploid (1EBN) genetic map generated with 99 RFLP markers revealed extensive synteny with previously published potato maps. A single dominant late-blight resistance locus (Rpi1) from S. pinnatisectum was mapped to chromosome 7, a region previously not associated with late-blight resistance. Characterization of the P.infestans isolate used for disease evaluations revealed that it possessed the avirulence gene corresponding to the R9 resistance locus, indicating that Rpi1 could possibly correspond to R9.</description><identifier>ISSN: 1617-4615</identifier><identifier>EISSN: 1617-4623</identifier><identifier>DOI: 10.1007/s004380100490</identifier><identifier>PMID: 11523789</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>AR9 gene ; chromosome 7 ; Chromosome Mapping ; Chromosomes ; Crosses, Genetic ; Cultivars ; Diploidy ; Fungi ; Genes ; Genetic Linkage ; Genetic Markers ; Genomics ; Immunity, Innate ; Lycopersicon esculentum - genetics ; Mexico ; Phenotype ; Phytophthora - pathogenicity ; Phytophthora infestans ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Plant introductions ; Ploidies ; R9 gene ; Rpi1 gene ; Solanaceae - genetics ; Solanaceae - physiology ; Solanum pinnatisectum ; Solanum tuberosum ; Solanum tuberosum - genetics</subject><ispartof>Molecular genetics and genomics : MGG, 2001-08, Vol.265 (6), p.977-985</ispartof><rights>Springer-Verlag 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-5bcd9b4b6342ef1e8ef6eb1a8b8e9bd6ee1cb0cb687ec70190baef6a259ee7f93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11523789$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kuhl, J C</creatorcontrib><creatorcontrib>Hanneman, Jr, R E</creatorcontrib><creatorcontrib>Havey, M J</creatorcontrib><title>Characterization and mapping of Rpi1, a late-blight resistance locus from diploid (1EBN) Mexican Solanum pinnatisectum</title><title>Molecular genetics and genomics : MGG</title><addtitle>Mol Genet Genomics</addtitle><description>Solanum is a diverse genus with over 200 species occupying a range of habitats from the Southwestern United States to Central Chile. Germplasm evaluations have focused on species that can be crossed with S. tuberosum, while Mexican diploid (2n = 2x = 24) Solanum species with an Endosperm Balance Number (EBN) of 1 have received less attention because of poor crossability due to their ploidy and EBN. Recent changes in Phytophthora infestans populations have increased the need for new sources of genetic resistance to this fungus. We have characterized resistance to P. infestans in the Mexican 2x(1EBN) species S. pinnatisectum. An interspecific hybrid between resistant S. pinnatisectum and susceptible S. cardiophyllum plants was backcrossed to S. cardiophyllum to generate a family segregating for late-blight resistance. The diploid (1EBN) genetic map generated with 99 RFLP markers revealed extensive synteny with previously published potato maps. A single dominant late-blight resistance locus (Rpi1) from S. pinnatisectum was mapped to chromosome 7, a region previously not associated with late-blight resistance. Characterization of the P.infestans isolate used for disease evaluations revealed that it possessed the avirulence gene corresponding to the R9 resistance locus, indicating that Rpi1 could possibly correspond to R9.</description><subject>AR9 gene</subject><subject>chromosome 7</subject><subject>Chromosome Mapping</subject><subject>Chromosomes</subject><subject>Crosses, Genetic</subject><subject>Cultivars</subject><subject>Diploidy</subject><subject>Fungi</subject><subject>Genes</subject><subject>Genetic Linkage</subject><subject>Genetic Markers</subject><subject>Genomics</subject><subject>Immunity, Innate</subject><subject>Lycopersicon esculentum - genetics</subject><subject>Mexico</subject><subject>Phenotype</subject><subject>Phytophthora - pathogenicity</subject><subject>Phytophthora infestans</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - microbiology</subject><subject>Plant introductions</subject><subject>Ploidies</subject><subject>R9 gene</subject><subject>Rpi1 gene</subject><subject>Solanaceae - genetics</subject><subject>Solanaceae - physiology</subject><subject>Solanum pinnatisectum</subject><subject>Solanum tuberosum</subject><subject>Solanum tuberosum - genetics</subject><issn>1617-4615</issn><issn>1617-4623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkUtLxTAQhYMoXl9LtxIQRMFqpmmbdqkXX3BV8LEuSTrVSNvUpBX11xvxoujG1ZzFN2ceh5BNYAfAmDj0jCU8Z0EnBVsgK5CBiJIs5ovfGtIJWfX-iTEQWSyWyQQgjbnIixXyMn2UTuoBnXmXg7EdlV1FW9n3pnugtqY3vYF9KmkjB4xUYx4eB-rQGz_ITiNtrB49rZ1taWX6xpqK7sLJ8dUevcRXo2VHb20ju7GlwbALEzzqYWzXyVItG48b87pG7k9P7qbn0ez67GJ6NIt0AskQpUpXhUpUxpMYa8Ac6wwVyFzlWKgqQwStmFZZLlALBgVTMiAyTgtEURd8jex8-fbOPo_oh7I1XmMTVkI7-lIAxEno_heEnMd5WvAAbv8Bn-zounBECQx4eLbIPu2iL0o7673DuuydaaV7C1D5mVv5K7fAb81dR9Vi9UPPg-IfqGGTdg</recordid><startdate>20010801</startdate><enddate>20010801</enddate><creator>Kuhl, J C</creator><creator>Hanneman, Jr, R E</creator><creator>Havey, M J</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20010801</creationdate><title>Characterization and mapping of Rpi1, a late-blight resistance locus from diploid (1EBN) Mexican Solanum pinnatisectum</title><author>Kuhl, J C ; Hanneman, Jr, R E ; Havey, M J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-5bcd9b4b6342ef1e8ef6eb1a8b8e9bd6ee1cb0cb687ec70190baef6a259ee7f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>AR9 gene</topic><topic>chromosome 7</topic><topic>Chromosome Mapping</topic><topic>Chromosomes</topic><topic>Crosses, Genetic</topic><topic>Cultivars</topic><topic>Diploidy</topic><topic>Fungi</topic><topic>Genes</topic><topic>Genetic Linkage</topic><topic>Genetic Markers</topic><topic>Genomics</topic><topic>Immunity, Innate</topic><topic>Lycopersicon esculentum - genetics</topic><topic>Mexico</topic><topic>Phenotype</topic><topic>Phytophthora - pathogenicity</topic><topic>Phytophthora infestans</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - microbiology</topic><topic>Plant introductions</topic><topic>Ploidies</topic><topic>R9 gene</topic><topic>Rpi1 gene</topic><topic>Solanaceae - genetics</topic><topic>Solanaceae - physiology</topic><topic>Solanum pinnatisectum</topic><topic>Solanum tuberosum</topic><topic>Solanum tuberosum - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuhl, J C</creatorcontrib><creatorcontrib>Hanneman, Jr, R E</creatorcontrib><creatorcontrib>Havey, M J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular genetics and genomics : MGG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuhl, J C</au><au>Hanneman, Jr, R E</au><au>Havey, M J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and mapping of Rpi1, a late-blight resistance locus from diploid (1EBN) Mexican Solanum pinnatisectum</atitle><jtitle>Molecular genetics and genomics : MGG</jtitle><addtitle>Mol Genet Genomics</addtitle><date>2001-08-01</date><risdate>2001</risdate><volume>265</volume><issue>6</issue><spage>977</spage><epage>985</epage><pages>977-985</pages><issn>1617-4615</issn><eissn>1617-4623</eissn><abstract>Solanum is a diverse genus with over 200 species occupying a range of habitats from the Southwestern United States to Central Chile. Germplasm evaluations have focused on species that can be crossed with S. tuberosum, while Mexican diploid (2n = 2x = 24) Solanum species with an Endosperm Balance Number (EBN) of 1 have received less attention because of poor crossability due to their ploidy and EBN. Recent changes in Phytophthora infestans populations have increased the need for new sources of genetic resistance to this fungus. We have characterized resistance to P. infestans in the Mexican 2x(1EBN) species S. pinnatisectum. An interspecific hybrid between resistant S. pinnatisectum and susceptible S. cardiophyllum plants was backcrossed to S. cardiophyllum to generate a family segregating for late-blight resistance. The diploid (1EBN) genetic map generated with 99 RFLP markers revealed extensive synteny with previously published potato maps. A single dominant late-blight resistance locus (Rpi1) from S. pinnatisectum was mapped to chromosome 7, a region previously not associated with late-blight resistance. Characterization of the P.infestans isolate used for disease evaluations revealed that it possessed the avirulence gene corresponding to the R9 resistance locus, indicating that Rpi1 could possibly correspond to R9.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>11523789</pmid><doi>10.1007/s004380100490</doi><tpages>9</tpages></addata></record> |
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| ispartof | Molecular genetics and genomics : MGG, 2001-08, Vol.265 (6), p.977-985 |
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| subjects | AR9 gene chromosome 7 Chromosome Mapping Chromosomes Crosses, Genetic Cultivars Diploidy Fungi Genes Genetic Linkage Genetic Markers Genomics Immunity, Innate Lycopersicon esculentum - genetics Mexico Phenotype Phytophthora - pathogenicity Phytophthora infestans Plant Diseases - genetics Plant Diseases - microbiology Plant introductions Ploidies R9 gene Rpi1 gene Solanaceae - genetics Solanaceae - physiology Solanum pinnatisectum Solanum tuberosum Solanum tuberosum - genetics |
| title | Characterization and mapping of Rpi1, a late-blight resistance locus from diploid (1EBN) Mexican Solanum pinnatisectum |
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