GANGLIOSIDE-MEDIATED METABOLIC SYNCHRONIZATION OF PROTEIN SYNTHESIS ACTIVITY IN CULTURED HEPATOCYTES
An ultradian oscillation of protein synthesis was detected by synchronization of metabolic activity in rat hepatocyte cultures. This oscillation occurs in dense cultures in fresh medium, but not in sparse ones. Metabolic synchronization of sparse cultures, however, was initiated by conditioned mediu...
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| Veröffentlicht in: | Cell biology international 2000-04, Vol.24 (4), p.211-222 |
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| creator | Brodsky, Vsevolod Y. Nechaeva, Natalia V. Zvezdina, Natalia D. Novikova, Tatjana E. Gvasava, Inna G. Fateeva, Valentina I. Prokazova, Nina V. Golovanova, Natalia K. |
| description | An ultradian oscillation of protein synthesis was detected by synchronization of metabolic activity in rat hepatocyte cultures. This oscillation occurs in dense cultures in fresh medium, but not in sparse ones. Metabolic synchronization of sparse cultures, however, was initiated by conditioned medium or addition of 0.3–0.5μm of a mixture of bovine brain gangliosides to fresh culture medium along with either 0.06–0.2μm GM1 or 0.1–0.2μm GDIa. GTIb and GDIb did not produce oscillations, nor did human liver ganglioside GM3. High expression of GM1 ganglioside determinants in hepatocytes maintained in the conditioned medium purified polyclonal antibodies to GM1 was coupled with protein synthetic oscillatory activity, i.e. metabolic synchronization. Incubation of dense cultures with GM1-antibodies for 24h decreased the amplitude of these oscillations. In sparse cultures maintained in fresh medium where protein synthesis showed no oscillatory pattern, GM1 expression was low. |
| doi_str_mv | 10.1006/cbir.1999.0505 |
| format | Article |
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This oscillation occurs in dense cultures in fresh medium, but not in sparse ones. Metabolic synchronization of sparse cultures, however, was initiated by conditioned medium or addition of 0.3–0.5μm of a mixture of bovine brain gangliosides to fresh culture medium along with either 0.06–0.2μm GM1 or 0.1–0.2μm GDIa. GTIb and GDIb did not produce oscillations, nor did human liver ganglioside GM3. High expression of GM1 ganglioside determinants in hepatocytes maintained in the conditioned medium purified polyclonal antibodies to GM1 was coupled with protein synthetic oscillatory activity, i.e. metabolic synchronization. Incubation of dense cultures with GM1-antibodies for 24h decreased the amplitude of these oscillations. In sparse cultures maintained in fresh medium where protein synthesis showed no oscillatory pattern, GM1 expression was low.</description><identifier>ISSN: 1065-6995</identifier><identifier>EISSN: 1095-8355</identifier><identifier>DOI: 10.1006/cbir.1999.0505</identifier><identifier>PMID: 10816322</identifier><language>eng</language><publisher>Oxford, UK: Elsevier Ltd</publisher><subject>Activity Cycles ; Animals ; Antibodies ; Cell Communication ; Cell Count ; Cells, Cultured ; Culture Media, Conditioned ; Culture Media, Serum-Free ; G(M1) Ganglioside - pharmacology ; G(M1) Ganglioside - physiology ; gangliosides ; Gangliosides - pharmacology ; Gangliosides - physiology ; hepatocyte culture ; Humans ; immunocytochemistry ; Kinetics ; Liver - cytology ; Liver - metabolism ; metabolic synchronization ; Protein Biosynthesis ; protein synthesis ; ultradian rhythms</subject><ispartof>Cell biology international, 2000-04, Vol.24 (4), p.211-222</ispartof><rights>2000 Academic Press</rights><rights>The Author(s) Journal compilation © 2000 International Federation for Cell Biology</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4222-1a09df3c29a79c868354936b3a92958208e0530bb1a68f4793c7182ca71d75f83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1006%2Fcbir.1999.0505$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1006%2Fcbir.1999.0505$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10816322$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brodsky, Vsevolod Y.</creatorcontrib><creatorcontrib>Nechaeva, Natalia V.</creatorcontrib><creatorcontrib>Zvezdina, Natalia D.</creatorcontrib><creatorcontrib>Novikova, Tatjana E.</creatorcontrib><creatorcontrib>Gvasava, Inna G.</creatorcontrib><creatorcontrib>Fateeva, Valentina I.</creatorcontrib><creatorcontrib>Prokazova, Nina V.</creatorcontrib><creatorcontrib>Golovanova, Natalia K.</creatorcontrib><title>GANGLIOSIDE-MEDIATED METABOLIC SYNCHRONIZATION OF PROTEIN SYNTHESIS ACTIVITY IN CULTURED HEPATOCYTES</title><title>Cell biology international</title><addtitle>Cell Biol Int</addtitle><description>An ultradian oscillation of protein synthesis was detected by synchronization of metabolic activity in rat hepatocyte cultures. This oscillation occurs in dense cultures in fresh medium, but not in sparse ones. Metabolic synchronization of sparse cultures, however, was initiated by conditioned medium or addition of 0.3–0.5μm of a mixture of bovine brain gangliosides to fresh culture medium along with either 0.06–0.2μm GM1 or 0.1–0.2μm GDIa. GTIb and GDIb did not produce oscillations, nor did human liver ganglioside GM3. High expression of GM1 ganglioside determinants in hepatocytes maintained in the conditioned medium purified polyclonal antibodies to GM1 was coupled with protein synthetic oscillatory activity, i.e. metabolic synchronization. Incubation of dense cultures with GM1-antibodies for 24h decreased the amplitude of these oscillations. In sparse cultures maintained in fresh medium where protein synthesis showed no oscillatory pattern, GM1 expression was low.</description><subject>Activity Cycles</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Cell Communication</subject><subject>Cell Count</subject><subject>Cells, Cultured</subject><subject>Culture Media, Conditioned</subject><subject>Culture Media, Serum-Free</subject><subject>G(M1) Ganglioside - pharmacology</subject><subject>G(M1) Ganglioside - physiology</subject><subject>gangliosides</subject><subject>Gangliosides - pharmacology</subject><subject>Gangliosides - physiology</subject><subject>hepatocyte culture</subject><subject>Humans</subject><subject>immunocytochemistry</subject><subject>Kinetics</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>metabolic synchronization</subject><subject>Protein Biosynthesis</subject><subject>protein synthesis</subject><subject>ultradian rhythms</subject><issn>1065-6995</issn><issn>1095-8355</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9v0zAYhi0EYmNw5Yhy4pbOP-LEPqap11pkydS4Q-ViJY4jBdp1s1tg__0SZZp2QZw-6_PzvtL3APAZwRmCML40Te9miHM-gxTSN-AcQU5DRih9O75jGsac0zPwwfufECIUsfg9OEOQoZhgfA7aZVosc1lWciHCa7GQqRKL4FqodF7mMguqbZGt1mUhf6RKlkVQXgU361IJWYxfaiUqWQVppuStVNtg2GabXG3WQ8dK3KSqzLZKVB_Bu67eefvpeV6AzZVQ2SrMy6XM0jw0EcY4RDXkbUcM5nXCDYuHKyJO4obUHHPKMGQWUgKbBtUx66KEE5Mghk2doDahHSMX4OvUe-8ODyfrj3rfe2N3u_rOHk5eJwhhFFEygLMJNO7gvbOdvnf9vnaPGkE9etWjVz161aPXIfDlufnU7G37Cp9EDgCZgD_9zj7-p05nc1lwOqbCKdX7o_37kqrdLx0nJKH6e7HU8zy_5eob1sXAs4m3g8XfvXXam97eGdv2zpqjbg_9vy54AjoXnI0</recordid><startdate>200004</startdate><enddate>200004</enddate><creator>Brodsky, Vsevolod Y.</creator><creator>Nechaeva, Natalia V.</creator><creator>Zvezdina, Natalia D.</creator><creator>Novikova, Tatjana E.</creator><creator>Gvasava, Inna G.</creator><creator>Fateeva, Valentina I.</creator><creator>Prokazova, Nina V.</creator><creator>Golovanova, Natalia K.</creator><general>Elsevier Ltd</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200004</creationdate><title>GANGLIOSIDE-MEDIATED METABOLIC SYNCHRONIZATION OF PROTEIN SYNTHESIS ACTIVITY IN CULTURED HEPATOCYTES</title><author>Brodsky, Vsevolod Y. ; Nechaeva, Natalia V. ; Zvezdina, Natalia D. ; Novikova, Tatjana E. ; Gvasava, Inna G. ; Fateeva, Valentina I. ; Prokazova, Nina V. ; Golovanova, Natalia K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4222-1a09df3c29a79c868354936b3a92958208e0530bb1a68f4793c7182ca71d75f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Activity Cycles</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Cell Communication</topic><topic>Cell Count</topic><topic>Cells, Cultured</topic><topic>Culture Media, Conditioned</topic><topic>Culture Media, Serum-Free</topic><topic>G(M1) Ganglioside - pharmacology</topic><topic>G(M1) Ganglioside - physiology</topic><topic>gangliosides</topic><topic>Gangliosides - pharmacology</topic><topic>Gangliosides - physiology</topic><topic>hepatocyte culture</topic><topic>Humans</topic><topic>immunocytochemistry</topic><topic>Kinetics</topic><topic>Liver - cytology</topic><topic>Liver - metabolism</topic><topic>metabolic synchronization</topic><topic>Protein Biosynthesis</topic><topic>protein synthesis</topic><topic>ultradian rhythms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brodsky, Vsevolod Y.</creatorcontrib><creatorcontrib>Nechaeva, Natalia V.</creatorcontrib><creatorcontrib>Zvezdina, Natalia D.</creatorcontrib><creatorcontrib>Novikova, Tatjana E.</creatorcontrib><creatorcontrib>Gvasava, Inna G.</creatorcontrib><creatorcontrib>Fateeva, Valentina I.</creatorcontrib><creatorcontrib>Prokazova, Nina V.</creatorcontrib><creatorcontrib>Golovanova, Natalia K.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brodsky, Vsevolod Y.</au><au>Nechaeva, Natalia V.</au><au>Zvezdina, Natalia D.</au><au>Novikova, Tatjana E.</au><au>Gvasava, Inna G.</au><au>Fateeva, Valentina I.</au><au>Prokazova, Nina V.</au><au>Golovanova, Natalia K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GANGLIOSIDE-MEDIATED METABOLIC SYNCHRONIZATION OF PROTEIN SYNTHESIS ACTIVITY IN CULTURED HEPATOCYTES</atitle><jtitle>Cell biology international</jtitle><addtitle>Cell Biol Int</addtitle><date>2000-04</date><risdate>2000</risdate><volume>24</volume><issue>4</issue><spage>211</spage><epage>222</epage><pages>211-222</pages><issn>1065-6995</issn><eissn>1095-8355</eissn><abstract>An ultradian oscillation of protein synthesis was detected by synchronization of metabolic activity in rat hepatocyte cultures. This oscillation occurs in dense cultures in fresh medium, but not in sparse ones. Metabolic synchronization of sparse cultures, however, was initiated by conditioned medium or addition of 0.3–0.5μm of a mixture of bovine brain gangliosides to fresh culture medium along with either 0.06–0.2μm GM1 or 0.1–0.2μm GDIa. GTIb and GDIb did not produce oscillations, nor did human liver ganglioside GM3. High expression of GM1 ganglioside determinants in hepatocytes maintained in the conditioned medium purified polyclonal antibodies to GM1 was coupled with protein synthetic oscillatory activity, i.e. metabolic synchronization. Incubation of dense cultures with GM1-antibodies for 24h decreased the amplitude of these oscillations. 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| subjects | Activity Cycles Animals Antibodies Cell Communication Cell Count Cells, Cultured Culture Media, Conditioned Culture Media, Serum-Free G(M1) Ganglioside - pharmacology G(M1) Ganglioside - physiology gangliosides Gangliosides - pharmacology Gangliosides - physiology hepatocyte culture Humans immunocytochemistry Kinetics Liver - cytology Liver - metabolism metabolic synchronization Protein Biosynthesis protein synthesis ultradian rhythms |
| title | GANGLIOSIDE-MEDIATED METABOLIC SYNCHRONIZATION OF PROTEIN SYNTHESIS ACTIVITY IN CULTURED HEPATOCYTES |
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