Method for screening peptide fragment ion mass spectra prior to database searching
A methodology is described for screening fragment ion spectra of peptides prior to database searching for protein identification. A software routine written in the Perl programming language was used to analyze data from previous Sequest database searches and develop a set of statistical descriptors...
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| Veröffentlicht in: | Journal of the American Society for Mass Spectrometry 2000-05, Vol.11 (5), p.422-426 |
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| container_end_page | 426 |
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| container_issue | 5 |
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| container_title | Journal of the American Society for Mass Spectrometry |
| container_volume | 11 |
| creator | Moore, Roger E. Young, Mary K. Lee, Terry D. |
| description | A methodology is described for screening fragment ion spectra of peptides prior to database searching for protein identification. A software routine written in the Perl programming language was used to analyze data from previous Sequest database searches and develop a set of statistical descriptors that could be used to identify spectra not likely to yield useful results in a database search. A second Perl program used an evolutionary algorithm to optimize the criteria for each statistical descriptor and generate a formula for determining spectral quality. This formula was used by a third Perl program to screen data sets from four independent liquid chromatography tandem mass spectrometry runs. On the average, use of the screening program reduced the time required for a database search by 1/2 with little loss of useful information from the database search results. |
| doi_str_mv | 10.1016/S1044-0305(00)00097-0 |
| format | Article |
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| identifier | ISSN: 1044-0305 |
| ispartof | Journal of the American Society for Mass Spectrometry, 2000-05, Vol.11 (5), p.422-426 |
| issn | 1044-0305 1879-1123 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; SpringerLink Journals - AutoHoldings |
| subjects | Algorithms Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Biological and medical sciences Databases, Factual Endopeptidases Evolutionary algorithms Fundamental and applied biological sciences. Psychology Hydrolysis Liquid chromatography Mass spectra Mass Spectrometry Peptide Library Peptides Peptides - chemistry Perl Programming languages Proteins Screening Searching Software Trypsin |
| title | Method for screening peptide fragment ion mass spectra prior to database searching |
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