Different in vivo localization of the Escherichia coli proteins CspD and CspA
Abstract Two Csp proteins (CspA and CspD) were fused to the green fluorescent protein GFP and expressed from their natural promoters or from an inducible promoter. Fluorescence microscopy and computerized image analysis indicate that in Escherichia coli growing at 37°C CspD localizes in the nucleoid...
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| Veröffentlicht in: | FEMS microbiology letters 2001-08, Vol.202 (2), p.171-176 |
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| container_title | FEMS microbiology letters |
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| creator | Giangrossi, Mara Exley, Rachel M. Le Hegarat, Françoise Pon, Cynthia L. |
| description | Abstract
Two Csp proteins (CspA and CspD) were fused to the green fluorescent protein GFP and expressed from their natural promoters or from an inducible promoter. Fluorescence microscopy and computerized image analysis indicate that in Escherichia coli growing at 37°C CspD localizes in the nucleoid like the control H-NS while CspA occupies a polar position away from the nucleoid. Following cold shock CspA maintains its location, while CspD is not sufficiently expressed to permit its localization. The different localization of CspA and CspD indicates that these proteins play different roles in the cell in spite of their extensive structural similarity. |
| doi_str_mv | 10.1111/j.1574-6968.2001.tb10799.x |
| format | Article |
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Two Csp proteins (CspA and CspD) were fused to the green fluorescent protein GFP and expressed from their natural promoters or from an inducible promoter. Fluorescence microscopy and computerized image analysis indicate that in Escherichia coli growing at 37°C CspD localizes in the nucleoid like the control H-NS while CspA occupies a polar position away from the nucleoid. Following cold shock CspA maintains its location, while CspD is not sufficiently expressed to permit its localization. The different localization of CspA and CspD indicates that these proteins play different roles in the cell in spite of their extensive structural similarity.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2001.tb10799.x</identifier><identifier>PMID: 11520610</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacteria ; Bacterial Proteins - analysis ; Bacterial Proteins - biosynthesis ; Bacteriology ; Biological and medical sciences ; Blotting, Western ; Cell Nucleolus ; Cold shock ; Cold shock protein A ; Cold shock protein D ; Cold Temperature ; CspA protein ; CspD protein ; E coli ; Escherichia coli ; Escherichia coli - metabolism ; Fluorescence ; Fluorescence microscopy ; Fundamental and applied biological sciences. Psychology ; Genetics ; Green fluorescent protein ; Green fluorescent protein fusion ; Green Fluorescent Proteins ; H‐NS ; Image analysis ; Image processing ; In vivo localization ; Localization ; Luminescent Proteins ; Microbiology ; Microscopy, Fluorescence ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Proteins ; Transcription Factors - analysis ; Transcription Factors - biosynthesis</subject><ispartof>FEMS microbiology letters, 2001-08, Vol.202 (2), p.171-176</ispartof><rights>2001 Federation of European Microbiological Societies 2001</rights><rights>2002 INIST-CNRS</rights><rights>2001 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3521-dded67b2a27575ebc4903f5bf5e77c89976f2e51fcf0071b2ac6869e22e0534e3</citedby><cites>FETCH-LOGICAL-c3521-dded67b2a27575ebc4903f5bf5e77c89976f2e51fcf0071b2ac6869e22e0534e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2001.tb10799.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2001.tb10799.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14165288$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11520610$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Giangrossi, Mara</creatorcontrib><creatorcontrib>Exley, Rachel M.</creatorcontrib><creatorcontrib>Le Hegarat, Françoise</creatorcontrib><creatorcontrib>Pon, Cynthia L.</creatorcontrib><title>Different in vivo localization of the Escherichia coli proteins CspD and CspA</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
Two Csp proteins (CspA and CspD) were fused to the green fluorescent protein GFP and expressed from their natural promoters or from an inducible promoter. Fluorescence microscopy and computerized image analysis indicate that in Escherichia coli growing at 37°C CspD localizes in the nucleoid like the control H-NS while CspA occupies a polar position away from the nucleoid. Following cold shock CspA maintains its location, while CspD is not sufficiently expressed to permit its localization. The different localization of CspA and CspD indicates that these proteins play different roles in the cell in spite of their extensive structural similarity.</description><subject>Bacteria</subject><subject>Bacterial Proteins - analysis</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell Nucleolus</subject><subject>Cold shock</subject><subject>Cold shock protein A</subject><subject>Cold shock protein D</subject><subject>Cold Temperature</subject><subject>CspA protein</subject><subject>CspD protein</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - metabolism</subject><subject>Fluorescence</subject><subject>Fluorescence microscopy</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Green fluorescent protein</subject><subject>Green fluorescent protein fusion</subject><subject>Green Fluorescent Proteins</subject><subject>H‐NS</subject><subject>Image analysis</subject><subject>Image processing</subject><subject>In vivo localization</subject><subject>Localization</subject><subject>Luminescent Proteins</subject><subject>Microbiology</subject><subject>Microscopy, Fluorescence</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Proteins</subject><subject>Transcription Factors - analysis</subject><subject>Transcription Factors - biosynthesis</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqVkd9r1TAUgIMo7m76L0hQ3FvrSdokrQ-DcbepcIcv-hzS9ISbS29zbdq5-debcosDUcS85EC-8ysfIa8Z5Cydd7ucCVVmspZVzgFYPjYMVF3n90_I6tfTU7KCQlUZg1qdkNMYdwBQcpDPyQljIgUMVuT2yjuHA_Yj9T2983eBdsGazv8wow89DY6OW6TX0W5x8HbrDbWh8_QwhBF9H-k6Hq6o6ds5uHxBnjnTRXy53Gfk6831l_XHbPP5w6f15SazheAsa1tspWq44UoogY0tayicaJxApWxV10o6joI56wAUS6CVlayRcwRRlFickfNj3TTGtwnjqPc-Wuw602OYolbpm5QsxT9BVkEtKgkJfPMbuAvT0KclNC8YAyELmKn3R8oOIcYBnT4Mfm-GB81Az270Ts8C9CxAz2704kbfp-RXS4up2WP7mLrISMDbBTAxKXCD6a2Pj1zJpOBVlbiLI_fdd_jwHyPom9sNUywVEMcCYTr8JT370wY_AdGTuZY</recordid><startdate>200108</startdate><enddate>200108</enddate><creator>Giangrossi, Mara</creator><creator>Exley, Rachel M.</creator><creator>Le Hegarat, Françoise</creator><creator>Pon, Cynthia L.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200108</creationdate><title>Different in vivo localization of the Escherichia coli proteins CspD and CspA</title><author>Giangrossi, Mara ; Exley, Rachel M. ; Le Hegarat, Françoise ; Pon, Cynthia L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3521-dded67b2a27575ebc4903f5bf5e77c89976f2e51fcf0071b2ac6869e22e0534e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Bacteria</topic><topic>Bacterial Proteins - analysis</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cell Nucleolus</topic><topic>Cold shock</topic><topic>Cold shock protein A</topic><topic>Cold shock protein D</topic><topic>Cold Temperature</topic><topic>CspA protein</topic><topic>CspD protein</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - metabolism</topic><topic>Fluorescence</topic><topic>Fluorescence microscopy</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Green fluorescent protein</topic><topic>Green fluorescent protein fusion</topic><topic>Green Fluorescent Proteins</topic><topic>H‐NS</topic><topic>Image analysis</topic><topic>Image processing</topic><topic>In vivo localization</topic><topic>Localization</topic><topic>Luminescent Proteins</topic><topic>Microbiology</topic><topic>Microscopy, Fluorescence</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Proteins</topic><topic>Transcription Factors - analysis</topic><topic>Transcription Factors - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Giangrossi, Mara</creatorcontrib><creatorcontrib>Exley, Rachel M.</creatorcontrib><creatorcontrib>Le Hegarat, Françoise</creatorcontrib><creatorcontrib>Pon, Cynthia L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Giangrossi, Mara</au><au>Exley, Rachel M.</au><au>Le Hegarat, Françoise</au><au>Pon, Cynthia L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Different in vivo localization of the Escherichia coli proteins CspD and CspA</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2001-08</date><risdate>2001</risdate><volume>202</volume><issue>2</issue><spage>171</spage><epage>176</epage><pages>171-176</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
Two Csp proteins (CspA and CspD) were fused to the green fluorescent protein GFP and expressed from their natural promoters or from an inducible promoter. Fluorescence microscopy and computerized image analysis indicate that in Escherichia coli growing at 37°C CspD localizes in the nucleoid like the control H-NS while CspA occupies a polar position away from the nucleoid. Following cold shock CspA maintains its location, while CspD is not sufficiently expressed to permit its localization. The different localization of CspA and CspD indicates that these proteins play different roles in the cell in spite of their extensive structural similarity.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>11520610</pmid><doi>10.1111/j.1574-6968.2001.tb10799.x</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2001-08, Vol.202 (2), p.171-176 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71117645 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Bacteria Bacterial Proteins - analysis Bacterial Proteins - biosynthesis Bacteriology Biological and medical sciences Blotting, Western Cell Nucleolus Cold shock Cold shock protein A Cold shock protein D Cold Temperature CspA protein CspD protein E coli Escherichia coli Escherichia coli - metabolism Fluorescence Fluorescence microscopy Fundamental and applied biological sciences. Psychology Genetics Green fluorescent protein Green fluorescent protein fusion Green Fluorescent Proteins H‐NS Image analysis Image processing In vivo localization Localization Luminescent Proteins Microbiology Microscopy, Fluorescence Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Proteins Transcription Factors - analysis Transcription Factors - biosynthesis |
| title | Different in vivo localization of the Escherichia coli proteins CspD and CspA |
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