Expression of matrix metalloprotease and tissue inhibitor of metalloprotease genes in human anterior cruciate ligament
Women are more susceptible to anterior cruciate ligament (ACL) injuries than men performing similar athletic activities. Because tissue remodeling may affect ligament strength, we assessed expression of tissue remodeling effector genes in the human ACL. Specifically, we surveyed ACL for RNAs encodin...
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| Veröffentlicht in: | Journal of orthopaedic research 2001-07, Vol.19 (4), p.642-649 |
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| creator | Foos, Marcus J Hickox, John R Mansour, Paul G Slauterbeck, James R Hardy, Daniel M |
| description | Women are more susceptible to anterior cruciate ligament (ACL) injuries than men performing similar athletic activities. Because tissue remodeling may affect ligament strength, we assessed expression of tissue remodeling effector genes in the human ACL. Specifically, we surveyed ACL for RNAs encoding all known matrix metalloproteases (MMPs) and tissue inhibitors of metalloproteases (TIMPs) by reverse transcription/polymerase chain reaction (RT-PCR). These experiments revealed that mRNAs encoding nine of sixteen MMPs and all four TIMPs are present in the normal ACL. The nine expressed proteases were MMPs 1–3, 7, 9, 11, 14, and 17 (collagenase 1, gelatinase A, stromelysin 1, matrilysin, gelatinase B, stromelysin 3, and membrane types 1 and 4, respectively), and MMP-18. Genes for MMPs 8, 10, 12, 13, 15, and 16 appeared not to be expressed in ACL, as their mRNAs were not detected using RT-PCR conditions that did yield positive signals from other tissues (testis or bone). We conclude that numerous genes encoding tissue remodeling effector proteins are expressed in the human ACL. |
| doi_str_mv | 10.1016/S0736-0266(00)00071-1 |
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Because tissue remodeling may affect ligament strength, we assessed expression of tissue remodeling effector genes in the human ACL. Specifically, we surveyed ACL for RNAs encoding all known matrix metalloproteases (MMPs) and tissue inhibitors of metalloproteases (TIMPs) by reverse transcription/polymerase chain reaction (RT-PCR). These experiments revealed that mRNAs encoding nine of sixteen MMPs and all four TIMPs are present in the normal ACL. The nine expressed proteases were MMPs 1–3, 7, 9, 11, 14, and 17 (collagenase 1, gelatinase A, stromelysin 1, matrilysin, gelatinase B, stromelysin 3, and membrane types 1 and 4, respectively), and MMP-18. Genes for MMPs 8, 10, 12, 13, 15, and 16 appeared not to be expressed in ACL, as their mRNAs were not detected using RT-PCR conditions that did yield positive signals from other tissues (testis or bone). We conclude that numerous genes encoding tissue remodeling effector proteins are expressed in the human ACL.</description><identifier>ISSN: 0736-0266</identifier><identifier>EISSN: 1554-527X</identifier><identifier>DOI: 10.1016/S0736-0266(00)00071-1</identifier><identifier>PMID: 11518274</identifier><identifier>CODEN: JOREDR</identifier><language>eng</language><publisher>Hoboken: Elsevier Ltd</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Anterior Cruciate Ligament - enzymology ; Anterior Cruciate Ligament - physiology ; Anterior Cruciate Ligament Injuries ; DNA Primers ; Female ; Gene Expression Regulation, Enzymologic ; Humans ; Male ; Matrix Metalloproteinases - genetics ; Middle Aged ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; Sex Factors ; Tissue Inhibitor of Metalloproteinases - genetics</subject><ispartof>Journal of orthopaedic research, 2001-07, Vol.19 (4), p.642-649</ispartof><rights>2001 Orthopaedic Research Society</rights><rights>Copyright © 2001 Orthopaedic Research Society</rights><rights>Copyright Journal of Bone and Joint Surgery, Inc. Jul 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6563-feb36c53f9be08cc60ca9a1d90950c623bb410aa28f3cef10c118e1fc3899d173</citedby><cites>FETCH-LOGICAL-c6563-feb36c53f9be08cc60ca9a1d90950c623bb410aa28f3cef10c118e1fc3899d173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0736-0266%2800%2900071-1$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2FS0736-0266%2800%2900071-1$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11518274$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Foos, Marcus J</creatorcontrib><creatorcontrib>Hickox, John R</creatorcontrib><creatorcontrib>Mansour, Paul G</creatorcontrib><creatorcontrib>Slauterbeck, James R</creatorcontrib><creatorcontrib>Hardy, Daniel M</creatorcontrib><title>Expression of matrix metalloprotease and tissue inhibitor of metalloprotease genes in human anterior cruciate ligament</title><title>Journal of orthopaedic research</title><addtitle>J. Orthop. Res</addtitle><description>Women are more susceptible to anterior cruciate ligament (ACL) injuries than men performing similar athletic activities. Because tissue remodeling may affect ligament strength, we assessed expression of tissue remodeling effector genes in the human ACL. Specifically, we surveyed ACL for RNAs encoding all known matrix metalloproteases (MMPs) and tissue inhibitors of metalloproteases (TIMPs) by reverse transcription/polymerase chain reaction (RT-PCR). These experiments revealed that mRNAs encoding nine of sixteen MMPs and all four TIMPs are present in the normal ACL. The nine expressed proteases were MMPs 1–3, 7, 9, 11, 14, and 17 (collagenase 1, gelatinase A, stromelysin 1, matrilysin, gelatinase B, stromelysin 3, and membrane types 1 and 4, respectively), and MMP-18. Genes for MMPs 8, 10, 12, 13, 15, and 16 appeared not to be expressed in ACL, as their mRNAs were not detected using RT-PCR conditions that did yield positive signals from other tissues (testis or bone). We conclude that numerous genes encoding tissue remodeling effector proteins are expressed in the human ACL.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Anterior Cruciate Ligament - enzymology</subject><subject>Anterior Cruciate Ligament - physiology</subject><subject>Anterior Cruciate Ligament Injuries</subject><subject>DNA Primers</subject><subject>Female</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Humans</subject><subject>Male</subject><subject>Matrix Metalloproteinases - genetics</subject><subject>Middle Aged</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>Sex Factors</subject><subject>Tissue Inhibitor of Metalloproteinases - genetics</subject><issn>0736-0266</issn><issn>1554-527X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqN0U1v1DAQBmALgei28BNAEQcEh8BMHDvJCVDVLqBVi_gQ3CzHmbQu-Vhsp93-e9zNqki9lNNcnnk1o5exZwhvEFC-_QYFlylkUr4CeA0ABab4gC1QiDwVWfHrIVvckj227_3FFmXlY7aHKLDMinzBLo82a0fe23FIxjbpdXB2k_QUdNeNazcG0p4SPTRJsN5PlNjh3NY2jG7L77gzGshHkpxPvR7iWiBnIzVuMlYHSjp7pnsawhP2qNWdp6e7ecB-HB99P_yYrk6Xnw4_rFIjheRpSzWXRvC2qglKYyQYXWlsKqgEGJnxus4RtM7KlhtqEQxiSdgaXlZVgwU_YC_n3Hjin4l8UL31hrpODzROXhWIWOQo74VYogAoRYQv7sCLcXJDfEJlXCDIeFpEYkbGjd47atXa2V67a4WgbupT2_rUTTcKQG2bURj3nu_Cp7qn5t_Wrq8I3s_gynZ0_X-p6vPpV0QArCDPeIxI5wjrA21uI7T7rWTBC6F-nixVVp4sV1X1RR1H_272FHu6tOSUN5YGQ411ZIJqRnvPV38BFyPM7g</recordid><startdate>200107</startdate><enddate>200107</enddate><creator>Foos, Marcus J</creator><creator>Hickox, John R</creator><creator>Mansour, Paul G</creator><creator>Slauterbeck, James R</creator><creator>Hardy, Daniel M</creator><general>Elsevier Ltd</general><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7TS</scope><scope>7X8</scope></search><sort><creationdate>200107</creationdate><title>Expression of matrix metalloprotease and tissue inhibitor of metalloprotease genes in human anterior cruciate ligament</title><author>Foos, Marcus J ; Hickox, John R ; Mansour, Paul G ; Slauterbeck, James R ; Hardy, Daniel M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6563-feb36c53f9be08cc60ca9a1d90950c623bb410aa28f3cef10c118e1fc3899d173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Anterior Cruciate Ligament - enzymology</topic><topic>Anterior Cruciate Ligament - physiology</topic><topic>Anterior Cruciate Ligament Injuries</topic><topic>DNA Primers</topic><topic>Female</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Humans</topic><topic>Male</topic><topic>Matrix Metalloproteinases - genetics</topic><topic>Middle Aged</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>Sex Factors</topic><topic>Tissue Inhibitor of Metalloproteinases - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Foos, Marcus J</creatorcontrib><creatorcontrib>Hickox, John R</creatorcontrib><creatorcontrib>Mansour, Paul G</creatorcontrib><creatorcontrib>Slauterbeck, James R</creatorcontrib><creatorcontrib>Hardy, Daniel M</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Physical Education Index</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of orthopaedic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Foos, Marcus J</au><au>Hickox, John R</au><au>Mansour, Paul G</au><au>Slauterbeck, James R</au><au>Hardy, Daniel M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of matrix metalloprotease and tissue inhibitor of metalloprotease genes in human anterior cruciate ligament</atitle><jtitle>Journal of orthopaedic research</jtitle><addtitle>J. Orthop. Res</addtitle><date>2001-07</date><risdate>2001</risdate><volume>19</volume><issue>4</issue><spage>642</spage><epage>649</epage><pages>642-649</pages><issn>0736-0266</issn><eissn>1554-527X</eissn><coden>JOREDR</coden><abstract>Women are more susceptible to anterior cruciate ligament (ACL) injuries than men performing similar athletic activities. Because tissue remodeling may affect ligament strength, we assessed expression of tissue remodeling effector genes in the human ACL. Specifically, we surveyed ACL for RNAs encoding all known matrix metalloproteases (MMPs) and tissue inhibitors of metalloproteases (TIMPs) by reverse transcription/polymerase chain reaction (RT-PCR). These experiments revealed that mRNAs encoding nine of sixteen MMPs and all four TIMPs are present in the normal ACL. The nine expressed proteases were MMPs 1–3, 7, 9, 11, 14, and 17 (collagenase 1, gelatinase A, stromelysin 1, matrilysin, gelatinase B, stromelysin 3, and membrane types 1 and 4, respectively), and MMP-18. Genes for MMPs 8, 10, 12, 13, 15, and 16 appeared not to be expressed in ACL, as their mRNAs were not detected using RT-PCR conditions that did yield positive signals from other tissues (testis or bone). We conclude that numerous genes encoding tissue remodeling effector proteins are expressed in the human ACL.</abstract><cop>Hoboken</cop><pub>Elsevier Ltd</pub><pmid>11518274</pmid><doi>10.1016/S0736-0266(00)00071-1</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Anterior Cruciate Ligament - enzymology Anterior Cruciate Ligament - physiology Anterior Cruciate Ligament Injuries DNA Primers Female Gene Expression Regulation, Enzymologic Humans Male Matrix Metalloproteinases - genetics Middle Aged Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis Sex Factors Tissue Inhibitor of Metalloproteinases - genetics |
| title | Expression of matrix metalloprotease and tissue inhibitor of metalloprotease genes in human anterior cruciate ligament |
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