Spontaneous galactosylation of agalactoglycoproteins in colostrum
We have found that spontaneous galactosylation of GlcNAc residues occurs in bovine colostrum, but not in dialyzed colostrum, without adding UDP-Gal as a donor substrate. UDP-Gal was shown to be present in bovine colostrum at a level ranging from 200 to 600 μM. When a tracer UDP-[ 14C]Gal was added t...
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| Veröffentlicht in: | FEBS letters 2000-05, Vol.473 (2), p.165-168 |
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| creator | Oubihi, Mohamed Kitajima, Ken Aoki, Naohito Matsuda, Tsukasa |
| description | We have found that spontaneous galactosylation of GlcNAc residues occurs in bovine colostrum, but not in dialyzed colostrum, without adding UDP-Gal as a donor substrate. UDP-Gal was shown to be present in bovine colostrum at a level ranging from 200 to 600 μM. When a tracer UDP-[
14C]Gal was added to the dialyzed colostrum together with a Gal β1,4-specific β-galactosidase, remarkable incorporation of radioactivity into 24–28 kDa and 33 kDa RCA1-positive glycoproteins was demonstrated by SDS–PAGE/autoradiography. Some 100–140 kDa agalactoglycoproteins of a CHO mutant cell line were also galactosylated on a blotted membrane by the incubation in the colostrum. |
| doi_str_mv | 10.1016/S0014-5793(00)01523-4 |
| format | Article |
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14C]Gal was added to the dialyzed colostrum together with a Gal β1,4-specific β-galactosidase, remarkable incorporation of radioactivity into 24–28 kDa and 33 kDa RCA1-positive glycoproteins was demonstrated by SDS–PAGE/autoradiography. Some 100–140 kDa agalactoglycoproteins of a CHO mutant cell line were also galactosylated on a blotted membrane by the incubation in the colostrum.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(00)01523-4</identifier><identifier>PMID: 10812067</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; beta-Galactosidase - metabolism ; Cattle ; Cell-Free System - metabolism ; CHO Cells ; Colostrum ; Colostrum - metabolism ; Cricetinae ; Female ; Galactose - metabolism ; Galactosylation ; Glycoprotein ; Glycoproteins - chemistry ; Glycoproteins - metabolism ; Kinetics ; Lactalbumin - metabolism ; Molecular Weight ; N-Acetyllactosamine Synthase - metabolism ; Pregnancy ; Substrate Specificity ; UDP-Gal ; Uridine Diphosphate Galactose - metabolism ; β-Galactosidase ; β1,4-Galactosyltransferase</subject><ispartof>FEBS letters, 2000-05, Vol.473 (2), p.165-168</ispartof><rights>2000 Federation of European Biochemical Societies</rights><rights>FEBS Letters 473 (2000) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4734-b437aa275450c3e54c44dbe2bb3ab5229b3c53b08a60f962ce0bd41c46fc3e073</citedby><cites>FETCH-LOGICAL-c4734-b437aa275450c3e54c44dbe2bb3ab5229b3c53b08a60f962ce0bd41c46fc3e073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2800%2901523-4$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-5793(00)01523-4$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,1433,3550,27924,27925,45574,45575,45995,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10812067$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oubihi, Mohamed</creatorcontrib><creatorcontrib>Kitajima, Ken</creatorcontrib><creatorcontrib>Aoki, Naohito</creatorcontrib><creatorcontrib>Matsuda, Tsukasa</creatorcontrib><title>Spontaneous galactosylation of agalactoglycoproteins in colostrum</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We have found that spontaneous galactosylation of GlcNAc residues occurs in bovine colostrum, but not in dialyzed colostrum, without adding UDP-Gal as a donor substrate. UDP-Gal was shown to be present in bovine colostrum at a level ranging from 200 to 600 μM. When a tracer UDP-[
14C]Gal was added to the dialyzed colostrum together with a Gal β1,4-specific β-galactosidase, remarkable incorporation of radioactivity into 24–28 kDa and 33 kDa RCA1-positive glycoproteins was demonstrated by SDS–PAGE/autoradiography. Some 100–140 kDa agalactoglycoproteins of a CHO mutant cell line were also galactosylated on a blotted membrane by the incubation in the colostrum.</description><subject>Animals</subject><subject>beta-Galactosidase - metabolism</subject><subject>Cattle</subject><subject>Cell-Free System - metabolism</subject><subject>CHO Cells</subject><subject>Colostrum</subject><subject>Colostrum - metabolism</subject><subject>Cricetinae</subject><subject>Female</subject><subject>Galactose - metabolism</subject><subject>Galactosylation</subject><subject>Glycoprotein</subject><subject>Glycoproteins - chemistry</subject><subject>Glycoproteins - metabolism</subject><subject>Kinetics</subject><subject>Lactalbumin - metabolism</subject><subject>Molecular Weight</subject><subject>N-Acetyllactosamine Synthase - metabolism</subject><subject>Pregnancy</subject><subject>Substrate Specificity</subject><subject>UDP-Gal</subject><subject>Uridine Diphosphate Galactose - metabolism</subject><subject>β-Galactosidase</subject><subject>β1,4-Galactosyltransferase</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkTFPwzAQhS0EoqXwE0CZEAyBc2zH6YRK1VKkSgyF2bIdpzJK4hInoPx7kqZCbDBZvvvu3dM7hC4x3GHA8f0GANOQ8Sm5AbgFzCIS0iM0xgknIaFxcozGP8gInXn_Dt0_wdNTNMKQ4AhiPkazzc6VtSyNa3ywlbnUtfNtLmvrysBlgTzUtnmr3a5ytbGlD2wZaJc7X1dNcY5OMpl7c3F4J-htuXidr8L1y9PzfLYONeWEhooSLmXEGWWgiWFUU5oqEylFpGJRNFVEM6IgkTFk0zjSBlRKsaZx1uHAyQRdD7qdi4_G-FoU1muT54N5wTHGDHPcgWwAdeW8r0wmdpUtZNUKDKLPTuyzE30wAkDssxO0m7s6LGhUYdJfU0NYHbAagC-bm_Z_qmK5eIz2nb7RX6Ar97seBinTJfZpTSW8tqbUJrWV0bVInf3D7Tfpw5LM</recordid><startdate>20000512</startdate><enddate>20000512</enddate><creator>Oubihi, Mohamed</creator><creator>Kitajima, Ken</creator><creator>Aoki, Naohito</creator><creator>Matsuda, Tsukasa</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000512</creationdate><title>Spontaneous galactosylation of agalactoglycoproteins in colostrum</title><author>Oubihi, Mohamed ; Kitajima, Ken ; Aoki, Naohito ; Matsuda, Tsukasa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4734-b437aa275450c3e54c44dbe2bb3ab5229b3c53b08a60f962ce0bd41c46fc3e073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>beta-Galactosidase - metabolism</topic><topic>Cattle</topic><topic>Cell-Free System - metabolism</topic><topic>CHO Cells</topic><topic>Colostrum</topic><topic>Colostrum - metabolism</topic><topic>Cricetinae</topic><topic>Female</topic><topic>Galactose - metabolism</topic><topic>Galactosylation</topic><topic>Glycoprotein</topic><topic>Glycoproteins - chemistry</topic><topic>Glycoproteins - metabolism</topic><topic>Kinetics</topic><topic>Lactalbumin - metabolism</topic><topic>Molecular Weight</topic><topic>N-Acetyllactosamine Synthase - metabolism</topic><topic>Pregnancy</topic><topic>Substrate Specificity</topic><topic>UDP-Gal</topic><topic>Uridine Diphosphate Galactose - metabolism</topic><topic>β-Galactosidase</topic><topic>β1,4-Galactosyltransferase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oubihi, Mohamed</creatorcontrib><creatorcontrib>Kitajima, Ken</creatorcontrib><creatorcontrib>Aoki, Naohito</creatorcontrib><creatorcontrib>Matsuda, Tsukasa</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oubihi, Mohamed</au><au>Kitajima, Ken</au><au>Aoki, Naohito</au><au>Matsuda, Tsukasa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spontaneous galactosylation of agalactoglycoproteins in colostrum</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2000-05-12</date><risdate>2000</risdate><volume>473</volume><issue>2</issue><spage>165</spage><epage>168</epage><pages>165-168</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>We have found that spontaneous galactosylation of GlcNAc residues occurs in bovine colostrum, but not in dialyzed colostrum, without adding UDP-Gal as a donor substrate. UDP-Gal was shown to be present in bovine colostrum at a level ranging from 200 to 600 μM. When a tracer UDP-[
14C]Gal was added to the dialyzed colostrum together with a Gal β1,4-specific β-galactosidase, remarkable incorporation of radioactivity into 24–28 kDa and 33 kDa RCA1-positive glycoproteins was demonstrated by SDS–PAGE/autoradiography. Some 100–140 kDa agalactoglycoproteins of a CHO mutant cell line were also galactosylated on a blotted membrane by the incubation in the colostrum.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>10812067</pmid><doi>10.1016/S0014-5793(00)01523-4</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Journals; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Alma/SFX Local Collection |
| subjects | Animals beta-Galactosidase - metabolism Cattle Cell-Free System - metabolism CHO Cells Colostrum Colostrum - metabolism Cricetinae Female Galactose - metabolism Galactosylation Glycoprotein Glycoproteins - chemistry Glycoproteins - metabolism Kinetics Lactalbumin - metabolism Molecular Weight N-Acetyllactosamine Synthase - metabolism Pregnancy Substrate Specificity UDP-Gal Uridine Diphosphate Galactose - metabolism β-Galactosidase β1,4-Galactosyltransferase |
| title | Spontaneous galactosylation of agalactoglycoproteins in colostrum |
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