Differentiation of Aedes triseriatus (Say) from Aedes hendersoni cockerell (Diptera : Culicidae) by restriction fragment length polymorphisms of amplified ribosomal dna
Aedes triseriatus is the primary vector of LaCrosse (LAC) virus, which can cause encephalitis, especially in young children. Aedes hendersoni, a sibling species of Ae. triseriatus, has a salivary gland barrier to LAC virus and, therefore, is not considered a vector of this virus. Adults of Ae. trise...
Gespeichert in:
| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2000-02, Vol.62 (2), p.193-199 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 199 |
|---|---|
| container_issue | 2 |
| container_start_page | 193 |
| container_title | The American journal of tropical medicine and hygiene |
| container_volume | 62 |
| creator | RENO, H. E VODKIN, M. H NOVAK, R. J |
| description | Aedes triseriatus is the primary vector of LaCrosse (LAC) virus, which can cause encephalitis, especially in young children. Aedes hendersoni, a sibling species of Ae. triseriatus, has a salivary gland barrier to LAC virus and, therefore, is not considered a vector of this virus. Adults of Ae. triseriatus are morphologically indistinguishable from those of Ae. hendersoni, and the two species are sympatric in the eastern United States. A definitive method of identifying field specimens is an important part of any disease surveillance program, particularly in the case of LAC virus. This study identifies restriction enzymes that produce species-specific restriction fragment length polymorphisms (RFLPs) from amplified ribosomal (r) DNA. In addition, sequences of the internal transcribed spacers 1 and 2 and the 5.8S regions of the rDNA were used to confirm the RFLP patterns. This study is the first to compare nucleotide sequences from Ae. triseriatus and Ae. hendersoni. |
| doi_str_mv | 10.4269/ajtmh.2000.62.193 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71108194</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17671390</sourcerecordid><originalsourceid>FETCH-LOGICAL-c400t-94f726e061c7661aad7955478e7a1ed38af935396d987fa99abc892f406883b03</originalsourceid><addsrcrecordid>eNqFkc9u1DAQxi0EokvhAbggHxBqD1ns2LHj3qptC0iVOABna-LYXZc4DnZy2DfiMXG6K8GN00gz3_zmz4fQW0q2vBbqIzzOYb-tCSFbUW-pYs_QhnIpKip48xxtSqGulGDyDL3K-ZEQ2taUvkRnlLSUcck26PeNd84mO84eZh9HHB2-tr3NeE4-21SyS8YX3-BwiV2K4VTc27G3KcfRYxPNzwIYBnxx46fZJsBXeLcM3vge7CXuDjjZXGjmie8SPIQyDg92fJj3eIrDIcQ07X0OeZ0OYRq887bHyXcxxwAD7kd4jV44GLJ9c4rn6Mfd7ffd5-r-66cvu-v7ynBC5kpxJ2thiaBGCkEBeqmahsvWSqC2Zy04xRqmRK9a6UAp6EyraseJaFvWEXaOPhy5U4q_lrK4Dj6bch6MNi5ZS7o-T_H_CqkUkjK1EulRaFLMOVmnp-QDpIOmRK8-6icf9eqjFrUuPpaedyf40gXb_9NxNK4I3p8EkA0M5auj8fmvrlzYCM7-AO1WqWw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17671390</pqid></control><display><type>article</type><title>Differentiation of Aedes triseriatus (Say) from Aedes hendersoni cockerell (Diptera : Culicidae) by restriction fragment length polymorphisms of amplified ribosomal dna</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>RENO, H. E ; VODKIN, M. H ; NOVAK, R. J</creator><creatorcontrib>RENO, H. E ; VODKIN, M. H ; NOVAK, R. J</creatorcontrib><description>Aedes triseriatus is the primary vector of LaCrosse (LAC) virus, which can cause encephalitis, especially in young children. Aedes hendersoni, a sibling species of Ae. triseriatus, has a salivary gland barrier to LAC virus and, therefore, is not considered a vector of this virus. Adults of Ae. triseriatus are morphologically indistinguishable from those of Ae. hendersoni, and the two species are sympatric in the eastern United States. A definitive method of identifying field specimens is an important part of any disease surveillance program, particularly in the case of LAC virus. This study identifies restriction enzymes that produce species-specific restriction fragment length polymorphisms (RFLPs) from amplified ribosomal (r) DNA. In addition, sequences of the internal transcribed spacers 1 and 2 and the 5.8S regions of the rDNA were used to confirm the RFLP patterns. This study is the first to compare nucleotide sequences from Ae. triseriatus and Ae. hendersoni.</description><identifier>ISSN: 0002-9637</identifier><identifier>EISSN: 1476-1645</identifier><identifier>DOI: 10.4269/ajtmh.2000.62.193</identifier><identifier>PMID: 10813473</identifier><identifier>CODEN: AJTHAB</identifier><language>eng</language><publisher>Lawrence, KS: Allen Press</publisher><subject>Aedes - chemistry ; Aedes - classification ; Aedes - genetics ; Aedes hendersoni ; Aedes triseriatus ; Animals ; Arboviral encephalitis ; Arboviroses ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; Culicidae ; DNA Primers - chemistry ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - isolation & purification ; Electrophoresis, Agar Gel ; Encephalitis, Viral - transmission ; Encephalitis, Viral - virology ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Human viral diseases ; Illinois ; Infectious diseases ; Insect Vectors - chemistry ; Insect Vectors - classification ; Insect Vectors - genetics ; La Crosse virus ; Medical sciences ; Medically important nuisances and vectors, pests of stored products and materials: population survey and control ; Molecular Sequence Data ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid ; Tropical medicine ; Tropical viral diseases ; USA ; Vectors. Intermediate hosts ; Viral diseases</subject><ispartof>The American journal of tropical medicine and hygiene, 2000-02, Vol.62 (2), p.193-199</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-94f726e061c7661aad7955478e7a1ed38af935396d987fa99abc892f406883b03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1396564$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10813473$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>RENO, H. E</creatorcontrib><creatorcontrib>VODKIN, M. H</creatorcontrib><creatorcontrib>NOVAK, R. J</creatorcontrib><title>Differentiation of Aedes triseriatus (Say) from Aedes hendersoni cockerell (Diptera : Culicidae) by restriction fragment length polymorphisms of amplified ribosomal dna</title><title>The American journal of tropical medicine and hygiene</title><addtitle>Am J Trop Med Hyg</addtitle><description>Aedes triseriatus is the primary vector of LaCrosse (LAC) virus, which can cause encephalitis, especially in young children. Aedes hendersoni, a sibling species of Ae. triseriatus, has a salivary gland barrier to LAC virus and, therefore, is not considered a vector of this virus. Adults of Ae. triseriatus are morphologically indistinguishable from those of Ae. hendersoni, and the two species are sympatric in the eastern United States. A definitive method of identifying field specimens is an important part of any disease surveillance program, particularly in the case of LAC virus. This study identifies restriction enzymes that produce species-specific restriction fragment length polymorphisms (RFLPs) from amplified ribosomal (r) DNA. In addition, sequences of the internal transcribed spacers 1 and 2 and the 5.8S regions of the rDNA were used to confirm the RFLP patterns. This study is the first to compare nucleotide sequences from Ae. triseriatus and Ae. hendersoni.</description><subject>Aedes - chemistry</subject><subject>Aedes - classification</subject><subject>Aedes - genetics</subject><subject>Aedes hendersoni</subject><subject>Aedes triseriatus</subject><subject>Animals</subject><subject>Arboviral encephalitis</subject><subject>Arboviroses</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Culicidae</subject><subject>DNA Primers - chemistry</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - isolation & purification</subject><subject>Electrophoresis, Agar Gel</subject><subject>Encephalitis, Viral - transmission</subject><subject>Encephalitis, Viral - virology</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human viral diseases</subject><subject>Illinois</subject><subject>Infectious diseases</subject><subject>Insect Vectors - chemistry</subject><subject>Insect Vectors - classification</subject><subject>Insect Vectors - genetics</subject><subject>La Crosse virus</subject><subject>Medical sciences</subject><subject>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Tropical medicine</subject><subject>Tropical viral diseases</subject><subject>USA</subject><subject>Vectors. Intermediate hosts</subject><subject>Viral diseases</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxi0EokvhAbggHxBqD1ns2LHj3qptC0iVOABna-LYXZc4DnZy2DfiMXG6K8GN00gz3_zmz4fQW0q2vBbqIzzOYb-tCSFbUW-pYs_QhnIpKip48xxtSqGulGDyDL3K-ZEQ2taUvkRnlLSUcck26PeNd84mO84eZh9HHB2-tr3NeE4-21SyS8YX3-BwiV2K4VTc27G3KcfRYxPNzwIYBnxx46fZJsBXeLcM3vge7CXuDjjZXGjmie8SPIQyDg92fJj3eIrDIcQ07X0OeZ0OYRq887bHyXcxxwAD7kd4jV44GLJ9c4rn6Mfd7ffd5-r-66cvu-v7ynBC5kpxJ2thiaBGCkEBeqmahsvWSqC2Zy04xRqmRK9a6UAp6EyraseJaFvWEXaOPhy5U4q_lrK4Dj6bch6MNi5ZS7o-T_H_CqkUkjK1EulRaFLMOVmnp-QDpIOmRK8-6icf9eqjFrUuPpaedyf40gXb_9NxNK4I3p8EkA0M5auj8fmvrlzYCM7-AO1WqWw</recordid><startdate>20000201</startdate><enddate>20000201</enddate><creator>RENO, H. E</creator><creator>VODKIN, M. H</creator><creator>NOVAK, R. J</creator><general>Allen Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7U9</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20000201</creationdate><title>Differentiation of Aedes triseriatus (Say) from Aedes hendersoni cockerell (Diptera : Culicidae) by restriction fragment length polymorphisms of amplified ribosomal dna</title><author>RENO, H. E ; VODKIN, M. H ; NOVAK, R. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-94f726e061c7661aad7955478e7a1ed38af935396d987fa99abc892f406883b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Aedes - chemistry</topic><topic>Aedes - classification</topic><topic>Aedes - genetics</topic><topic>Aedes hendersoni</topic><topic>Aedes triseriatus</topic><topic>Animals</topic><topic>Arboviral encephalitis</topic><topic>Arboviroses</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Culicidae</topic><topic>DNA Primers - chemistry</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - isolation & purification</topic><topic>Electrophoresis, Agar Gel</topic><topic>Encephalitis, Viral - transmission</topic><topic>Encephalitis, Viral - virology</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Human viral diseases</topic><topic>Illinois</topic><topic>Infectious diseases</topic><topic>Insect Vectors - chemistry</topic><topic>Insect Vectors - classification</topic><topic>Insect Vectors - genetics</topic><topic>La Crosse virus</topic><topic>Medical sciences</topic><topic>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Tropical medicine</topic><topic>Tropical viral diseases</topic><topic>USA</topic><topic>Vectors. Intermediate hosts</topic><topic>Viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RENO, H. E</creatorcontrib><creatorcontrib>VODKIN, M. H</creatorcontrib><creatorcontrib>NOVAK, R. J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of tropical medicine and hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>RENO, H. E</au><au>VODKIN, M. H</au><au>NOVAK, R. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differentiation of Aedes triseriatus (Say) from Aedes hendersoni cockerell (Diptera : Culicidae) by restriction fragment length polymorphisms of amplified ribosomal dna</atitle><jtitle>The American journal of tropical medicine and hygiene</jtitle><addtitle>Am J Trop Med Hyg</addtitle><date>2000-02-01</date><risdate>2000</risdate><volume>62</volume><issue>2</issue><spage>193</spage><epage>199</epage><pages>193-199</pages><issn>0002-9637</issn><eissn>1476-1645</eissn><coden>AJTHAB</coden><abstract>Aedes triseriatus is the primary vector of LaCrosse (LAC) virus, which can cause encephalitis, especially in young children. Aedes hendersoni, a sibling species of Ae. triseriatus, has a salivary gland barrier to LAC virus and, therefore, is not considered a vector of this virus. Adults of Ae. triseriatus are morphologically indistinguishable from those of Ae. hendersoni, and the two species are sympatric in the eastern United States. A definitive method of identifying field specimens is an important part of any disease surveillance program, particularly in the case of LAC virus. This study identifies restriction enzymes that produce species-specific restriction fragment length polymorphisms (RFLPs) from amplified ribosomal (r) DNA. In addition, sequences of the internal transcribed spacers 1 and 2 and the 5.8S regions of the rDNA were used to confirm the RFLP patterns. This study is the first to compare nucleotide sequences from Ae. triseriatus and Ae. hendersoni.</abstract><cop>Lawrence, KS</cop><pub>Allen Press</pub><pmid>10813473</pmid><doi>10.4269/ajtmh.2000.62.193</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0002-9637 |
| ispartof | The American journal of tropical medicine and hygiene, 2000-02, Vol.62 (2), p.193-199 |
| issn | 0002-9637 1476-1645 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71108194 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Aedes - chemistry Aedes - classification Aedes - genetics Aedes hendersoni Aedes triseriatus Animals Arboviral encephalitis Arboviroses Base Sequence Biological and medical sciences Cloning, Molecular Culicidae DNA Primers - chemistry DNA, Ribosomal - chemistry DNA, Ribosomal - isolation & purification Electrophoresis, Agar Gel Encephalitis, Viral - transmission Encephalitis, Viral - virology Freshwater Fundamental and applied biological sciences. Psychology Human viral diseases Illinois Infectious diseases Insect Vectors - chemistry Insect Vectors - classification Insect Vectors - genetics La Crosse virus Medical sciences Medically important nuisances and vectors, pests of stored products and materials: population survey and control Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Sequence Alignment Sequence Analysis, DNA Sequence Homology, Nucleic Acid Tropical medicine Tropical viral diseases USA Vectors. Intermediate hosts Viral diseases |
| title | Differentiation of Aedes triseriatus (Say) from Aedes hendersoni cockerell (Diptera : Culicidae) by restriction fragment length polymorphisms of amplified ribosomal dna |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T19%3A59%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differentiation%20of%20Aedes%20triseriatus%20(Say)%20from%20Aedes%20hendersoni%20cockerell%20(Diptera%20:%20Culicidae)%20by%20restriction%20fragment%20length%20polymorphisms%20of%20amplified%20ribosomal%20dna&rft.jtitle=The%20American%20journal%20of%20tropical%20medicine%20and%20hygiene&rft.au=RENO,%20H.%20E&rft.date=2000-02-01&rft.volume=62&rft.issue=2&rft.spage=193&rft.epage=199&rft.pages=193-199&rft.issn=0002-9637&rft.eissn=1476-1645&rft.coden=AJTHAB&rft_id=info:doi/10.4269/ajtmh.2000.62.193&rft_dat=%3Cproquest_cross%3E17671390%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17671390&rft_id=info:pmid/10813473&rfr_iscdi=true |