Determination of Molecular Self-Diffusion Coefficient Using Multiple Spin-Echo NMR Spectroscopy with Removal of Convection and Background Gradient Artifacts
A new approach is presented for the measurement of the self-diffusion coefficients of molecules in solution. It has been applied to metabolites in biofluids such as seminal and blood plasma at physiological temperature. The method is based on the double-gradient-spin-echo pulse sequence in which CPM...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2001-08, Vol.73 (15), p.3528-3534 |
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| creator | Zhang, Xu Li, Cong-Gang Ye, Chao-Hui Liu, Mai-Li |
| description | A new approach is presented for the measurement of the self-diffusion coefficients of molecules in solution. It has been applied to metabolites in biofluids such as seminal and blood plasma at physiological temperature. The method is based on the double-gradient-spin-echo pulse sequence in which CPMG and bipolar gradient pulses have been implemented. The double-gradient spin-echo is shown to be useful in reducing the thermal convection that can cause over-estimation of the diffusion coefficients. The multiple spin-echoes in association with the CPMG approach is also insensitive to background gradient artifacts. In addition, the CPMG sequence enables longer diffusion periods (up to seconds) to be used without phase distortion; therefore, the proposed method is suitable for determining the diffusion coefficients of small metabolites in biofluids, where the resonances of large molecules, such as proteins, are suppressed during the spin-echo period as a result of their fast relaxation. |
| doi_str_mv | 10.1021/ac0101104 |
| format | Article |
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It has been applied to metabolites in biofluids such as seminal and blood plasma at physiological temperature. The method is based on the double-gradient-spin-echo pulse sequence in which CPMG and bipolar gradient pulses have been implemented. The double-gradient spin-echo is shown to be useful in reducing the thermal convection that can cause over-estimation of the diffusion coefficients. The multiple spin-echoes in association with the CPMG approach is also insensitive to background gradient artifacts. In addition, the CPMG sequence enables longer diffusion periods (up to seconds) to be used without phase distortion; therefore, the proposed method is suitable for determining the diffusion coefficients of small metabolites in biofluids, where the resonances of large molecules, such as proteins, are suppressed during the spin-echo period as a result of their fast relaxation.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac0101104</identifier><identifier>PMID: 11510814</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Amino Acids - analysis ; Amino Acids - blood ; Analytical chemistry ; Biological and medical sciences ; Blood ; Chemistry ; Diffusion ; Electron Spin Resonance Spectroscopy ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Glucose - analysis ; Glycoproteins - analysis ; Glycoproteins - blood ; Humans ; Lipoproteins - analysis ; Lipoproteins - blood ; Male ; Metabolites ; Methods ; Molecular and cellular biology ; Molecules ; NMR ; Nuclear magnetic resonance ; Plasmas ; Proteins ; Semen - chemistry ; Solutions ; Spectrometric and optical methods ; Spectroscopy</subject><ispartof>Analytical chemistry (Washington), 2001-08, Vol.73 (15), p.3528-3534</ispartof><rights>Copyright © 2001 American Chemical Society</rights><rights>2002 INIST-CNRS</rights><rights>Copyright American Chemical Society Aug 1, 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a437t-d78a1033cec52a04b2a2f653bb0efc09738b168abbb05a312af8ae63693ebdfc3</citedby><cites>FETCH-LOGICAL-a437t-d78a1033cec52a04b2a2f653bb0efc09738b168abbb05a312af8ae63693ebdfc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac0101104$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac0101104$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,777,781,2752,27057,27905,27906,56719,56769</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14159071$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11510814$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Xu</creatorcontrib><creatorcontrib>Li, Cong-Gang</creatorcontrib><creatorcontrib>Ye, Chao-Hui</creatorcontrib><creatorcontrib>Liu, Mai-Li</creatorcontrib><title>Determination of Molecular Self-Diffusion Coefficient Using Multiple Spin-Echo NMR Spectroscopy with Removal of Convection and Background Gradient Artifacts</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>A new approach is presented for the measurement of the self-diffusion coefficients of molecules in solution. It has been applied to metabolites in biofluids such as seminal and blood plasma at physiological temperature. The method is based on the double-gradient-spin-echo pulse sequence in which CPMG and bipolar gradient pulses have been implemented. The double-gradient spin-echo is shown to be useful in reducing the thermal convection that can cause over-estimation of the diffusion coefficients. The multiple spin-echoes in association with the CPMG approach is also insensitive to background gradient artifacts. In addition, the CPMG sequence enables longer diffusion periods (up to seconds) to be used without phase distortion; therefore, the proposed method is suitable for determining the diffusion coefficients of small metabolites in biofluids, where the resonances of large molecules, such as proteins, are suppressed during the spin-echo period as a result of their fast relaxation.</description><subject>Amino Acids - analysis</subject><subject>Amino Acids - blood</subject><subject>Analytical chemistry</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Chemistry</subject><subject>Diffusion</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - analysis</subject><subject>Glycoproteins - analysis</subject><subject>Glycoproteins - blood</subject><subject>Humans</subject><subject>Lipoproteins - analysis</subject><subject>Lipoproteins - blood</subject><subject>Male</subject><subject>Metabolites</subject><subject>Methods</subject><subject>Molecular and cellular biology</subject><subject>Molecules</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Plasmas</subject><subject>Proteins</subject><subject>Semen - chemistry</subject><subject>Solutions</subject><subject>Spectrometric and optical methods</subject><subject>Spectroscopy</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdtu1DAQhiMEokvhghdAFhJIXATsOKe9bNNSEF0O3fbamjjj1q0TB9sp9F14WBx21ZXggisf_k__zPyTJM8Zfctoxt6BpIwyRvMHyYIVGU3Lus4eJgtKKU-zitK95In313RmWPk42WOsYLRm-SL5dYQBXa8HCNoOxCqysgblZMCRNRqVHmmlJj9rjUWltNQ4BHLh9XBJVpMJejRI1qMe0mN5Zcnn1Vl8oQzOemnHO_JDhytyhr29BTPbN3a4jfJsCENHDkHeXDo7xeuJg-6P-YELWoEM_mnySIHx-Gx77icX74_Pmw_p6ZeTj83BaQo5r0LaVTUwyrlEWWRA8zaDTJUFb1uKStJlxeuWlTW08aMAzjJQNWDJyyXHtlOS7yevN76js98n9EH02ks0Bga0kxfVnG0dS_wPzFhexGRZBF_-BV7byQ1xiMhUdVHyOo_Qmw0kY1jeoRKj0z24O8GomBcr7hcb2Rdbw6ntsduR201G4NUWAC_BKAeD1H7H5axY0mruLN1w2gf8ea-DuxFlxatCnH9di8Os-LT6tmxEufMF6XdD_Nvgb0NKxoE</recordid><startdate>20010801</startdate><enddate>20010801</enddate><creator>Zhang, Xu</creator><creator>Li, Cong-Gang</creator><creator>Ye, Chao-Hui</creator><creator>Liu, Mai-Li</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20010801</creationdate><title>Determination of Molecular Self-Diffusion Coefficient Using Multiple Spin-Echo NMR Spectroscopy with Removal of Convection and Background Gradient Artifacts</title><author>Zhang, Xu ; Li, Cong-Gang ; Ye, Chao-Hui ; Liu, Mai-Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a437t-d78a1033cec52a04b2a2f653bb0efc09738b168abbb05a312af8ae63693ebdfc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino Acids - analysis</topic><topic>Amino Acids - blood</topic><topic>Analytical chemistry</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Chemistry</topic><topic>Diffusion</topic><topic>Electron Spin Resonance Spectroscopy</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. 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In addition, the CPMG sequence enables longer diffusion periods (up to seconds) to be used without phase distortion; therefore, the proposed method is suitable for determining the diffusion coefficients of small metabolites in biofluids, where the resonances of large molecules, such as proteins, are suppressed during the spin-echo period as a result of their fast relaxation.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>11510814</pmid><doi>10.1021/ac0101104</doi><tpages>7</tpages></addata></record> |
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| subjects | Amino Acids - analysis Amino Acids - blood Analytical chemistry Biological and medical sciences Blood Chemistry Diffusion Electron Spin Resonance Spectroscopy Exact sciences and technology Fundamental and applied biological sciences. Psychology Glucose - analysis Glycoproteins - analysis Glycoproteins - blood Humans Lipoproteins - analysis Lipoproteins - blood Male Metabolites Methods Molecular and cellular biology Molecules NMR Nuclear magnetic resonance Plasmas Proteins Semen - chemistry Solutions Spectrometric and optical methods Spectroscopy |
| title | Determination of Molecular Self-Diffusion Coefficient Using Multiple Spin-Echo NMR Spectroscopy with Removal of Convection and Background Gradient Artifacts |
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