Expression of HDL Receptor, CLA-1 in Human Smooth-Muscle Cells and Effect of Interferon-γ on its Regulation
High-density lipoprotein (HDL) exerts antiatherogenic effects by various mechanisms. The protective effect of HDL is thought to involve the reverse transport of cholesterol from cells in the arterial wall to the liver for disposal. We previously identified human scavenger receptor BI (hSR-BI/CLA-1)...
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| Veröffentlicht in: | Hormone and metabolic research 2001-07, Vol.33 (7), p.389-393 |
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| creator | Imachi, H. Murao, K. Cao, W. M. Ohyama, T. Sato, M. Sasaguri, Y. Ishida, T. Takahara, J. |
| description | High-density lipoprotein (HDL) exerts antiatherogenic effects by various mechanisms. The protective effect of HDL is thought to involve the reverse transport of cholesterol from cells in the arterial wall to the liver for disposal. We previously identified human scavenger receptor BI (hSR-BI/CLA-1) as a receptor for human HDL, but did not examine the expression of hSR-BI/CLA-1 in smooth-muscle cells. In this present study, a human aortic intima smooth-muscle cell line immortalized with SV 40 DNA was established, and the expression of hSR-BI/CLA-1 in this cell line analyzed by Western blot and RT-PCR. HSR-BI/CLA-1 mRNA and protein were detected in both this cell line and primary human aortic smooth-muscle cells. A cytokine, interferon-gamma (IFN-γ) inhibited the hSR-BI/CLA-1 protein expression, but not mRNA expression. This observation confirmed that selective cholesterol ester uptake from HDL was inhibited by IFN-γ. These results indicated that hSR-BI/CLA-1 may be expressed in human smooth-muscle cells, and the expression may be modulated by IFN-γ. HSR-BI/CLA-1 on smooth-muscle cells could play an important role in atherogenesis. |
| doi_str_mv | 10.1055/s-2001-16237 |
| format | Article |
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M. ; Ohyama, T. ; Sato, M. ; Sasaguri, Y. ; Ishida, T. ; Takahara, J.</creator><creatorcontrib>Imachi, H. ; Murao, K. ; Cao, W. M. ; Ohyama, T. ; Sato, M. ; Sasaguri, Y. ; Ishida, T. ; Takahara, J.</creatorcontrib><description>High-density lipoprotein (HDL) exerts antiatherogenic effects by various mechanisms. The protective effect of HDL is thought to involve the reverse transport of cholesterol from cells in the arterial wall to the liver for disposal. We previously identified human scavenger receptor BI (hSR-BI/CLA-1) as a receptor for human HDL, but did not examine the expression of hSR-BI/CLA-1 in smooth-muscle cells. In this present study, a human aortic intima smooth-muscle cell line immortalized with SV 40 DNA was established, and the expression of hSR-BI/CLA-1 in this cell line analyzed by Western blot and RT-PCR. HSR-BI/CLA-1 mRNA and protein were detected in both this cell line and primary human aortic smooth-muscle cells. A cytokine, interferon-gamma (IFN-γ) inhibited the hSR-BI/CLA-1 protein expression, but not mRNA expression. This observation confirmed that selective cholesterol ester uptake from HDL was inhibited by IFN-γ. These results indicated that hSR-BI/CLA-1 may be expressed in human smooth-muscle cells, and the expression may be modulated by IFN-γ. HSR-BI/CLA-1 on smooth-muscle cells could play an important role in atherogenesis.</description><identifier>ISSN: 0018-5043</identifier><identifier>EISSN: 1439-4286</identifier><identifier>DOI: 10.1055/s-2001-16237</identifier><identifier>PMID: 11507674</identifier><language>eng</language><publisher>Germany</publisher><subject>Aorta, Thoracic - cytology ; Aorta, Thoracic - drug effects ; Aorta, Thoracic - metabolism ; Blotting, Western ; Carrier Proteins ; Cells, Cultured ; Cholesterol, HDL - metabolism ; Cytokines - biosynthesis ; Gene Expression Regulation - drug effects ; Humans ; Interferon-gamma - pharmacology ; Lipoproteins, HDL - metabolism ; Membrane Proteins ; Muscle, Smooth - cytology ; Muscle, Smooth - metabolism ; Original Basic ; Receptors, Immunologic - biosynthesis ; Receptors, Immunologic - genetics ; Receptors, Lipoprotein - biosynthesis ; Receptors, Lipoprotein - genetics ; Receptors, Scavenger ; Recombinant Proteins ; RNA-Binding Proteins ; Scavenger Receptors, Class B ; Transforming Growth Factor beta - pharmacology</subject><ispartof>Hormone and metabolic research, 2001-07, Vol.33 (7), p.389-393</ispartof><rights>Georg Thieme Verlag Stuttgart · New York</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c323t-682ef4ae90a59fa241b5d9a7080b89518e8206650d213884008930f2a9bc2d863</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-2001-16237.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><linktohtml>$$Uhttps://www.thieme-connect.de/products/ejournals/html/10.1055/s-2001-16237$$EHTML$$P50$$Gthieme$$H</linktohtml><link.rule.ids>314,776,780,3004,3005,27901,27902,54534,54535</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11507674$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Imachi, H.</creatorcontrib><creatorcontrib>Murao, K.</creatorcontrib><creatorcontrib>Cao, W. 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In this present study, a human aortic intima smooth-muscle cell line immortalized with SV 40 DNA was established, and the expression of hSR-BI/CLA-1 in this cell line analyzed by Western blot and RT-PCR. HSR-BI/CLA-1 mRNA and protein were detected in both this cell line and primary human aortic smooth-muscle cells. A cytokine, interferon-gamma (IFN-γ) inhibited the hSR-BI/CLA-1 protein expression, but not mRNA expression. This observation confirmed that selective cholesterol ester uptake from HDL was inhibited by IFN-γ. These results indicated that hSR-BI/CLA-1 may be expressed in human smooth-muscle cells, and the expression may be modulated by IFN-γ. HSR-BI/CLA-1 on smooth-muscle cells could play an important role in atherogenesis.</description><subject>Aorta, Thoracic - cytology</subject><subject>Aorta, Thoracic - drug effects</subject><subject>Aorta, Thoracic - metabolism</subject><subject>Blotting, Western</subject><subject>Carrier Proteins</subject><subject>Cells, Cultured</subject><subject>Cholesterol, HDL - metabolism</subject><subject>Cytokines - biosynthesis</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humans</subject><subject>Interferon-gamma - pharmacology</subject><subject>Lipoproteins, HDL - metabolism</subject><subject>Membrane Proteins</subject><subject>Muscle, Smooth - cytology</subject><subject>Muscle, Smooth - metabolism</subject><subject>Original Basic</subject><subject>Receptors, Immunologic - biosynthesis</subject><subject>Receptors, Immunologic - genetics</subject><subject>Receptors, Lipoprotein - biosynthesis</subject><subject>Receptors, Lipoprotein - genetics</subject><subject>Receptors, Scavenger</subject><subject>Recombinant Proteins</subject><subject>RNA-Binding Proteins</subject><subject>Scavenger Receptors, Class B</subject><subject>Transforming Growth Factor beta - pharmacology</subject><issn>0018-5043</issn><issn>1439-4286</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE1P3DAQhi0EYhfaG2fkE5di8GfiHFG6dJEWIfXjbHmTMRuUxIvtSOV39X_wm-rtrtQLJ2usZ56ZeRG6YPSGUaVuI-GUMsIKLsojNGdSVERyXRyjef7XRFEpZugsxpdcyorJUzRjTNGyKOUc9Yvf2wAxdn7E3uHl1xX-Dg1skw_XuF7dEYa7ES-nwY74x-B92pDHKTY94Br6PmI7tnjhHDRp1_4wJggOgh_J-x-clV2K2fc89TblCZ_QibN9hM-H9xz9ul_8rJdk9fTtob5bkUZwkUihOThpoaJWVc5yydaqrWxJNV3rSjENmtOiULTlTGgtKdWVoI7bat3wVhfiHF3tvdvgXyeIyQxdbPK-dgQ_RVMyRiUTKoPXe7AJPsYAzmxDN9jwZhg1u3RNNLt0zb90M3558E7rAdr_8CHODHzZA2nTwQDmxU9hzJd-rPsLX-uAmA</recordid><startdate>20010701</startdate><enddate>20010701</enddate><creator>Imachi, H.</creator><creator>Murao, K.</creator><creator>Cao, W. 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In this present study, a human aortic intima smooth-muscle cell line immortalized with SV 40 DNA was established, and the expression of hSR-BI/CLA-1 in this cell line analyzed by Western blot and RT-PCR. HSR-BI/CLA-1 mRNA and protein were detected in both this cell line and primary human aortic smooth-muscle cells. A cytokine, interferon-gamma (IFN-γ) inhibited the hSR-BI/CLA-1 protein expression, but not mRNA expression. This observation confirmed that selective cholesterol ester uptake from HDL was inhibited by IFN-γ. These results indicated that hSR-BI/CLA-1 may be expressed in human smooth-muscle cells, and the expression may be modulated by IFN-γ. HSR-BI/CLA-1 on smooth-muscle cells could play an important role in atherogenesis.</abstract><cop>Germany</cop><pmid>11507674</pmid><doi>10.1055/s-2001-16237</doi><tpages>5</tpages></addata></record> |
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| subjects | Aorta, Thoracic - cytology Aorta, Thoracic - drug effects Aorta, Thoracic - metabolism Blotting, Western Carrier Proteins Cells, Cultured Cholesterol, HDL - metabolism Cytokines - biosynthesis Gene Expression Regulation - drug effects Humans Interferon-gamma - pharmacology Lipoproteins, HDL - metabolism Membrane Proteins Muscle, Smooth - cytology Muscle, Smooth - metabolism Original Basic Receptors, Immunologic - biosynthesis Receptors, Immunologic - genetics Receptors, Lipoprotein - biosynthesis Receptors, Lipoprotein - genetics Receptors, Scavenger Recombinant Proteins RNA-Binding Proteins Scavenger Receptors, Class B Transforming Growth Factor beta - pharmacology |
| title | Expression of HDL Receptor, CLA-1 in Human Smooth-Muscle Cells and Effect of Interferon-γ on its Regulation |
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