Simultaneous determination of alpha-, gamma-tocopherol and their quinones in rats plasma and tissues using reversed-phase high-performance liquid chromatography
We established a method to determine simultaneously alpha- and gamma-tocopherol (-Toc) and their quinones (alpha-TQ and rho-gamma-TQ) in biological samples by reverse-phase high-performance liquid chromatography (HPLC). Tocs had a shorter retention time than TQs, and alpha-forms had a shorter retent...
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| Veröffentlicht in: | Journal of Nutritional Science and Vitaminology 2001, Vol.47(2), pp.102-107 |
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| container_title | Journal of Nutritional Science and Vitaminology |
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| creator | Kiyose, C. (Ochanomizu Univ., Tokyo (Japan). Inst. of Environmental Science for Human Life) Saito, H Ueda, T Igarashi, O |
| description | We established a method to determine simultaneously alpha- and gamma-tocopherol (-Toc) and their quinones (alpha-TQ and rho-gamma-TQ) in biological samples by reverse-phase high-performance liquid chromatography (HPLC). Tocs had a shorter retention time than TQs, and alpha-forms had a shorter retention times than gamma-forms. Four peaks of Tocs and TQs were completely separated by this method. Subsequently, we investigated the distribution of alpha-, gamma-Toc and TQ in rat tissues and the excretion of Tocs and TQs in rat bile by the above HPLC method. Rats deficient in vitamin E were divided into two groups, gamma-Toc group and alpha+gamma-Toc group, and tissues were collected at 6 and 24 h after intravenous administration of Tocs. Also, bile collection was started immediately and performed at 3 h intervals during 24 h after intravenous administration. The concentration of alpha- and gamma-Toc and their quinones in plasma, tissues and bile were determined by this method. gamma-Toc concentration in the liver of alpha+gamma-Toc group was higher than that of gamma-Toc group. However, rho-gamma-TQ in the liver was not significantly different between alpha+gamma-Toc group and gamma-Toc group. Also, both alpha-TQ and rho-gamma-TQ were present in very low concentrations in all tissues. Therefore, we suggested that the distribution of gamma-Toc is affected by alpha-Toc present in vivo, but the oxidative production of rho-gamma-TQ from gamma-Toc is not affected. |
| doi_str_mv | 10.3177/jnsv.47.102 |
| format | Article |
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(Ochanomizu Univ., Tokyo (Japan). Inst. of Environmental Science for Human Life) ; Saito, H ; Ueda, T ; Igarashi, O</creator><creatorcontrib>Kiyose, C. (Ochanomizu Univ., Tokyo (Japan). Inst. of Environmental Science for Human Life) ; Saito, H ; Ueda, T ; Igarashi, O</creatorcontrib><description>We established a method to determine simultaneously alpha- and gamma-tocopherol (-Toc) and their quinones (alpha-TQ and rho-gamma-TQ) in biological samples by reverse-phase high-performance liquid chromatography (HPLC). Tocs had a shorter retention time than TQs, and alpha-forms had a shorter retention times than gamma-forms. Four peaks of Tocs and TQs were completely separated by this method. Subsequently, we investigated the distribution of alpha-, gamma-Toc and TQ in rat tissues and the excretion of Tocs and TQs in rat bile by the above HPLC method. Rats deficient in vitamin E were divided into two groups, gamma-Toc group and alpha+gamma-Toc group, and tissues were collected at 6 and 24 h after intravenous administration of Tocs. Also, bile collection was started immediately and performed at 3 h intervals during 24 h after intravenous administration. The concentration of alpha- and gamma-Toc and their quinones in plasma, tissues and bile were determined by this method. gamma-Toc concentration in the liver of alpha+gamma-Toc group was higher than that of gamma-Toc group. However, rho-gamma-TQ in the liver was not significantly different between alpha+gamma-Toc group and gamma-Toc group. Also, both alpha-TQ and rho-gamma-TQ were present in very low concentrations in all tissues. Therefore, we suggested that the distribution of gamma-Toc is affected by alpha-Toc present in vivo, but the oxidative production of rho-gamma-TQ from gamma-Toc is not affected.</description><identifier>ISSN: 0301-4800</identifier><identifier>EISSN: 1881-7742</identifier><identifier>DOI: 10.3177/jnsv.47.102</identifier><identifier>PMID: 11508699</identifier><language>eng</language><publisher>Tokyo: Center for Academic Publications Japan</publisher><subject>alpha-Tocopherol - analysis ; alpha-Tocopherol - blood ; ANALYTICAL METHODS ; ANIMAL TISSUES ; Animals ; Antioxidants - analysis ; Bile - metabolism ; Biological and medical sciences ; BLOOD PLASMA ; Calibration ; Chromatography, High Pressure Liquid - methods ; Fundamental and applied biological sciences. Psychology ; gamma-Tocopherol - analysis ; gamma-Tocopherol - blood ; General aspects ; HPLC ; HPLC method ; Injections, Intravenous ; Male ; p-γ-tocopherylquinone ; QUINONES ; Quinones - analysis ; Quinones - blood ; RATS ; Rats, Sprague-Dawley ; Reference Standards ; Time Factors ; Tissue Distribution ; TOCOPHEROLS ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Vitamin E Deficiency - blood ; α-tocopherol ; α-tocopherylquinone ; γ-tocopherol</subject><ispartof>Journal of Nutritional Science and Vitaminology, 2001, Vol.47(2), pp.102-107</ispartof><rights>the Center for Academic Publications Japan</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-9f4c674c84898571e465a2b42e47e670d3bd25cb34caa0886a1c0d29fedffd2c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,1884,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1109230$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11508699$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kiyose, C. (Ochanomizu Univ., Tokyo (Japan). Inst. of Environmental Science for Human Life)</creatorcontrib><creatorcontrib>Saito, H</creatorcontrib><creatorcontrib>Ueda, T</creatorcontrib><creatorcontrib>Igarashi, O</creatorcontrib><title>Simultaneous determination of alpha-, gamma-tocopherol and their quinones in rats plasma and tissues using reversed-phase high-performance liquid chromatography</title><title>Journal of Nutritional Science and Vitaminology</title><addtitle>J Nutr Sci Vitaminol</addtitle><description>We established a method to determine simultaneously alpha- and gamma-tocopherol (-Toc) and their quinones (alpha-TQ and rho-gamma-TQ) in biological samples by reverse-phase high-performance liquid chromatography (HPLC). Tocs had a shorter retention time than TQs, and alpha-forms had a shorter retention times than gamma-forms. Four peaks of Tocs and TQs were completely separated by this method. Subsequently, we investigated the distribution of alpha-, gamma-Toc and TQ in rat tissues and the excretion of Tocs and TQs in rat bile by the above HPLC method. Rats deficient in vitamin E were divided into two groups, gamma-Toc group and alpha+gamma-Toc group, and tissues were collected at 6 and 24 h after intravenous administration of Tocs. Also, bile collection was started immediately and performed at 3 h intervals during 24 h after intravenous administration. The concentration of alpha- and gamma-Toc and their quinones in plasma, tissues and bile were determined by this method. gamma-Toc concentration in the liver of alpha+gamma-Toc group was higher than that of gamma-Toc group. However, rho-gamma-TQ in the liver was not significantly different between alpha+gamma-Toc group and gamma-Toc group. Also, both alpha-TQ and rho-gamma-TQ were present in very low concentrations in all tissues. Therefore, we suggested that the distribution of gamma-Toc is affected by alpha-Toc present in vivo, but the oxidative production of rho-gamma-TQ from gamma-Toc is not affected.</description><subject>alpha-Tocopherol - analysis</subject><subject>alpha-Tocopherol - blood</subject><subject>ANALYTICAL METHODS</subject><subject>ANIMAL TISSUES</subject><subject>Animals</subject><subject>Antioxidants - analysis</subject><subject>Bile - metabolism</subject><subject>Biological and medical sciences</subject><subject>BLOOD PLASMA</subject><subject>Calibration</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma-Tocopherol - analysis</subject><subject>gamma-Tocopherol - blood</subject><subject>General aspects</subject><subject>HPLC</subject><subject>HPLC method</subject><subject>Injections, Intravenous</subject><subject>Male</subject><subject>p-γ-tocopherylquinone</subject><subject>QUINONES</subject><subject>Quinones - analysis</subject><subject>Quinones - blood</subject><subject>RATS</subject><subject>Rats, Sprague-Dawley</subject><subject>Reference Standards</subject><subject>Time Factors</subject><subject>Tissue Distribution</subject><subject>TOCOPHEROLS</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Vitamin E Deficiency - blood</subject><subject>α-tocopherol</subject><subject>α-tocopherylquinone</subject><subject>γ-tocopherol</subject><issn>0301-4800</issn><issn>1881-7742</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkU-L1TAUxYsozji6cq1kIW60zyRNm3Q5jP8ZUFDX5b70ts0jTTpJOjDfxo9qnn2MQkgI58c5yT1F8ZzRXcWkfHdw8XYn5I5R_qA4Z0qxUkrBHxbntKKsFIrSs-JJjAdKRauEelycMVZT1bTtefH7h5lXm8ChXyPpMWGYjYNkvCN-IGCXCcq3ZIR5hjJ57ZcJg7cEXE_ShCaQm9U47zAS40iAFMliIc6wESbGNUtrNG4kAW8xROzL7BmRTGacygXD4MMMTiOxJnv1RE_Bz5D8GGCZ7p4WjwawEZ-dzovi18cPP68-l9ffPn25urwutahVKttB6EYKnf_XqloyFE0NfC84ComNpH2173mt95XQAFSpBpimPW8H7Ieh57q6KF5vvkvwN_nNqZtN1GjtNppOMkaZYiyDbzZQBx9jwKFbgpkh3HWMdsdCumMhnZD5zjP98mS77mfs_7GnBjLw6gRA1GCHkEdh4n8cbXlFM_Z-ww4xwYj3OoRktMW_mayV1TGXb1uOv5f1BKFDl21ebDYD-A7GkJO-fueU5kWbmld_AC8-uZk</recordid><startdate>20010401</startdate><enddate>20010401</enddate><creator>Kiyose, C. (Ochanomizu Univ., Tokyo (Japan). Inst. of Environmental Science for Human Life)</creator><creator>Saito, H</creator><creator>Ueda, T</creator><creator>Igarashi, O</creator><general>Center for Academic Publications Japan</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010401</creationdate><title>Simultaneous determination of alpha-, gamma-tocopherol and their quinones in rats plasma and tissues using reversed-phase high-performance liquid chromatography</title><author>Kiyose, C. (Ochanomizu Univ., Tokyo (Japan). Inst. of Environmental Science for Human Life) ; Saito, H ; Ueda, T ; Igarashi, O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-9f4c674c84898571e465a2b42e47e670d3bd25cb34caa0886a1c0d29fedffd2c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>alpha-Tocopherol - analysis</topic><topic>alpha-Tocopherol - blood</topic><topic>ANALYTICAL METHODS</topic><topic>ANIMAL TISSUES</topic><topic>Animals</topic><topic>Antioxidants - analysis</topic><topic>Bile - metabolism</topic><topic>Biological and medical sciences</topic><topic>BLOOD PLASMA</topic><topic>Calibration</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma-Tocopherol - analysis</topic><topic>gamma-Tocopherol - blood</topic><topic>General aspects</topic><topic>HPLC</topic><topic>HPLC method</topic><topic>Injections, Intravenous</topic><topic>Male</topic><topic>p-γ-tocopherylquinone</topic><topic>QUINONES</topic><topic>Quinones - analysis</topic><topic>Quinones - blood</topic><topic>RATS</topic><topic>Rats, Sprague-Dawley</topic><topic>Reference Standards</topic><topic>Time Factors</topic><topic>Tissue Distribution</topic><topic>TOCOPHEROLS</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Vitamin E Deficiency - blood</topic><topic>α-tocopherol</topic><topic>α-tocopherylquinone</topic><topic>γ-tocopherol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kiyose, C. (Ochanomizu Univ., Tokyo (Japan). Inst. of Environmental Science for Human Life)</creatorcontrib><creatorcontrib>Saito, H</creatorcontrib><creatorcontrib>Ueda, T</creatorcontrib><creatorcontrib>Igarashi, O</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Nutritional Science and Vitaminology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kiyose, C. (Ochanomizu Univ., Tokyo (Japan). Inst. of Environmental Science for Human Life)</au><au>Saito, H</au><au>Ueda, T</au><au>Igarashi, O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous determination of alpha-, gamma-tocopherol and their quinones in rats plasma and tissues using reversed-phase high-performance liquid chromatography</atitle><jtitle>Journal of Nutritional Science and Vitaminology</jtitle><addtitle>J Nutr Sci Vitaminol</addtitle><date>2001-04-01</date><risdate>2001</risdate><volume>47</volume><issue>2</issue><spage>102</spage><epage>107</epage><pages>102-107</pages><issn>0301-4800</issn><eissn>1881-7742</eissn><abstract>We established a method to determine simultaneously alpha- and gamma-tocopherol (-Toc) and their quinones (alpha-TQ and rho-gamma-TQ) in biological samples by reverse-phase high-performance liquid chromatography (HPLC). Tocs had a shorter retention time than TQs, and alpha-forms had a shorter retention times than gamma-forms. Four peaks of Tocs and TQs were completely separated by this method. Subsequently, we investigated the distribution of alpha-, gamma-Toc and TQ in rat tissues and the excretion of Tocs and TQs in rat bile by the above HPLC method. Rats deficient in vitamin E were divided into two groups, gamma-Toc group and alpha+gamma-Toc group, and tissues were collected at 6 and 24 h after intravenous administration of Tocs. Also, bile collection was started immediately and performed at 3 h intervals during 24 h after intravenous administration. The concentration of alpha- and gamma-Toc and their quinones in plasma, tissues and bile were determined by this method. gamma-Toc concentration in the liver of alpha+gamma-Toc group was higher than that of gamma-Toc group. However, rho-gamma-TQ in the liver was not significantly different between alpha+gamma-Toc group and gamma-Toc group. Also, both alpha-TQ and rho-gamma-TQ were present in very low concentrations in all tissues. Therefore, we suggested that the distribution of gamma-Toc is affected by alpha-Toc present in vivo, but the oxidative production of rho-gamma-TQ from gamma-Toc is not affected.</abstract><cop>Tokyo</cop><pub>Center for Academic Publications Japan</pub><pmid>11508699</pmid><doi>10.3177/jnsv.47.102</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | alpha-Tocopherol - analysis alpha-Tocopherol - blood ANALYTICAL METHODS ANIMAL TISSUES Animals Antioxidants - analysis Bile - metabolism Biological and medical sciences BLOOD PLASMA Calibration Chromatography, High Pressure Liquid - methods Fundamental and applied biological sciences. Psychology gamma-Tocopherol - analysis gamma-Tocopherol - blood General aspects HPLC HPLC method Injections, Intravenous Male p-γ-tocopherylquinone QUINONES Quinones - analysis Quinones - blood RATS Rats, Sprague-Dawley Reference Standards Time Factors Tissue Distribution TOCOPHEROLS Vertebrates: anatomy and physiology, studies on body, several organs or systems Vitamin E Deficiency - blood α-tocopherol α-tocopherylquinone γ-tocopherol |
| title | Simultaneous determination of alpha-, gamma-tocopherol and their quinones in rats plasma and tissues using reversed-phase high-performance liquid chromatography |
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