Similar myeloid recovery despite superior overall engraftment in NOD/SCID mice after transplantation of human CD34+ cells from umbilical cord blood as compared to adult sources
Umbilical cord blood (UCB), bone marrow (BM) and mobilized peripheral blood (mPB) are used as sources of hematopoietic stem cells for transplantation. The NOD/SCID mouse model was used to compare the lineage-specific repopulating potential of CD34(+) cells derived from these sources. Six to 8 weeks...
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| creator | NOORT, W. A WILPSHAAR, J HERTOGH, C. D. P RAD, M LURVINK, E. G. A VAN LUXEMBURG-HEIJS, S. A. P ZWINDERMAN, K VERWEY, R. A WILLEMZE, R FALKENBURG, J. H. F |
| description | Umbilical cord blood (UCB), bone marrow (BM) and mobilized peripheral blood (mPB) are used as sources of hematopoietic stem cells for transplantation. The NOD/SCID mouse model was used to compare the lineage-specific repopulating potential of CD34(+) cells derived from these sources. Six to 8 weeks after transplantation, blood, BM, spleen, liver and thymus, were harvested, and analyzed by flow cytometry using CD34, CD45, myeloid, and lymphoid lineage-specific antibodies. Fifty percent engraftment of human cells in bone marrow of mice was estimated to be reached with 0.55 x 10(6) CD34(+) UCB cells or with 7.9 x 10(6) CD34(+) cells from adult sources, illustrating a 10-fold superiority of UCB CD34(+) cells to engraft NOD/SCID mice. Lineage-specific characterization of engrafted human cells showed that the high engraftment potential of CD34(+) cells from UCB was due to a preferential B cell development (2-81%). In contrast, comparable percentages of myeloid cells were found following transplantation of CD34(+) cells from UCB, BM and mPB (1-38%), and occurred at significant levels only at relatively high doses. Since the CD34 content of UCB transplants is usually at least one log lower than of transplant from adult sources, these results correspond to the clinical findings with UCB transplantation showing a relatively high overall engraftment, but delayed myeloid recovery. |
| doi_str_mv | 10.1038/sj.bmt.1703120 |
| format | Article |
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A ; WILPSHAAR, J ; HERTOGH, C. D. P ; RAD, M ; LURVINK, E. G. A ; VAN LUXEMBURG-HEIJS, S. A. P ; ZWINDERMAN, K ; VERWEY, R. A ; WILLEMZE, R ; FALKENBURG, J. H. F</creator><creatorcontrib>NOORT, W. A ; WILPSHAAR, J ; HERTOGH, C. D. P ; RAD, M ; LURVINK, E. G. A ; VAN LUXEMBURG-HEIJS, S. A. P ; ZWINDERMAN, K ; VERWEY, R. A ; WILLEMZE, R ; FALKENBURG, J. H. F</creatorcontrib><description>Umbilical cord blood (UCB), bone marrow (BM) and mobilized peripheral blood (mPB) are used as sources of hematopoietic stem cells for transplantation. The NOD/SCID mouse model was used to compare the lineage-specific repopulating potential of CD34(+) cells derived from these sources. Six to 8 weeks after transplantation, blood, BM, spleen, liver and thymus, were harvested, and analyzed by flow cytometry using CD34, CD45, myeloid, and lymphoid lineage-specific antibodies. Fifty percent engraftment of human cells in bone marrow of mice was estimated to be reached with 0.55 x 10(6) CD34(+) UCB cells or with 7.9 x 10(6) CD34(+) cells from adult sources, illustrating a 10-fold superiority of UCB CD34(+) cells to engraft NOD/SCID mice. Lineage-specific characterization of engrafted human cells showed that the high engraftment potential of CD34(+) cells from UCB was due to a preferential B cell development (2-81%). In contrast, comparable percentages of myeloid cells were found following transplantation of CD34(+) cells from UCB, BM and mPB (1-38%), and occurred at significant levels only at relatively high doses. Since the CD34 content of UCB transplants is usually at least one log lower than of transplant from adult sources, these results correspond to the clinical findings with UCB transplantation showing a relatively high overall engraftment, but delayed myeloid recovery.</description><identifier>ISSN: 0268-3369</identifier><identifier>EISSN: 1476-5365</identifier><identifier>DOI: 10.1038/sj.bmt.1703120</identifier><identifier>PMID: 11509934</identifier><identifier>CODEN: BMTRE9</identifier><language>eng</language><publisher>Basingstoke: Nature Publishing Group</publisher><subject>Adult ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; Antibodies ; Antigens, CD - blood ; Antigens, CD34 - blood ; Biological and medical sciences ; Blood ; Bone marrow ; Bone Marrow Cells - cytology ; Bone Marrow Cells - immunology ; Bone marrow transplantation ; Bone marrow, stem cells transplantation. Graft versus host reaction ; CD34 antigen ; CD45 antigen ; Cord blood ; Engraftment ; Fetal Blood - cytology ; Flow Cytometry ; Hematopoietic Stem Cell Transplantation ; Hematopoietic stem cells ; Humans ; Infant, Newborn ; Liver - cytology ; Liver - immunology ; Medical sciences ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Myeloid cells ; Peripheral blood ; Recovery ; Spleen ; Spleen - cytology ; Spleen - immunology ; Stem cell transplantation ; Stem cells ; Thymus Gland - cytology ; Thymus Gland - immunology ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy ; Transplantation ; Transplantation, Heterologous - physiology ; Transplants ; Transplants & implants ; Umbilical cord</subject><ispartof>Bone marrow transplantation (Basingstoke), 2001-07, Vol.28 (2), p.163-171</ispartof><rights>2002 INIST-CNRS</rights><rights>COPYRIGHT 2001 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jul 2001</rights><rights>Macmillan Publishers Limited 2001.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-73db22cc5e9884ddd28e8d6b5a9e621aa9ee8cf66e259fd33d362fb717034343</citedby><cites>FETCH-LOGICAL-c474t-73db22cc5e9884ddd28e8d6b5a9e621aa9ee8cf66e259fd33d362fb717034343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14101334$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11509934$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NOORT, W. A</creatorcontrib><creatorcontrib>WILPSHAAR, J</creatorcontrib><creatorcontrib>HERTOGH, C. D. P</creatorcontrib><creatorcontrib>RAD, M</creatorcontrib><creatorcontrib>LURVINK, E. G. A</creatorcontrib><creatorcontrib>VAN LUXEMBURG-HEIJS, S. A. P</creatorcontrib><creatorcontrib>ZWINDERMAN, K</creatorcontrib><creatorcontrib>VERWEY, R. A</creatorcontrib><creatorcontrib>WILLEMZE, R</creatorcontrib><creatorcontrib>FALKENBURG, J. H. F</creatorcontrib><title>Similar myeloid recovery despite superior overall engraftment in NOD/SCID mice after transplantation of human CD34+ cells from umbilical cord blood as compared to adult sources</title><title>Bone marrow transplantation (Basingstoke)</title><addtitle>Bone Marrow Transplant</addtitle><description>Umbilical cord blood (UCB), bone marrow (BM) and mobilized peripheral blood (mPB) are used as sources of hematopoietic stem cells for transplantation. The NOD/SCID mouse model was used to compare the lineage-specific repopulating potential of CD34(+) cells derived from these sources. Six to 8 weeks after transplantation, blood, BM, spleen, liver and thymus, were harvested, and analyzed by flow cytometry using CD34, CD45, myeloid, and lymphoid lineage-specific antibodies. Fifty percent engraftment of human cells in bone marrow of mice was estimated to be reached with 0.55 x 10(6) CD34(+) UCB cells or with 7.9 x 10(6) CD34(+) cells from adult sources, illustrating a 10-fold superiority of UCB CD34(+) cells to engraft NOD/SCID mice. Lineage-specific characterization of engrafted human cells showed that the high engraftment potential of CD34(+) cells from UCB was due to a preferential B cell development (2-81%). In contrast, comparable percentages of myeloid cells were found following transplantation of CD34(+) cells from UCB, BM and mPB (1-38%), and occurred at significant levels only at relatively high doses. Since the CD34 content of UCB transplants is usually at least one log lower than of transplant from adult sources, these results correspond to the clinical findings with UCB transplantation showing a relatively high overall engraftment, but delayed myeloid recovery.</description><subject>Adult</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antigens, CD - blood</subject><subject>Antigens, CD34 - blood</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - immunology</subject><subject>Bone marrow transplantation</subject><subject>Bone marrow, stem cells transplantation. Graft versus host reaction</subject><subject>CD34 antigen</subject><subject>CD45 antigen</subject><subject>Cord blood</subject><subject>Engraftment</subject><subject>Fetal Blood - cytology</subject><subject>Flow Cytometry</subject><subject>Hematopoietic Stem Cell Transplantation</subject><subject>Hematopoietic stem cells</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Liver - cytology</subject><subject>Liver - immunology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred NOD</subject><subject>Mice, SCID</subject><subject>Myeloid cells</subject><subject>Peripheral blood</subject><subject>Recovery</subject><subject>Spleen</subject><subject>Spleen - cytology</subject><subject>Spleen - immunology</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Thymus Gland - cytology</subject><subject>Thymus Gland - immunology</subject><subject>Transfusions. Complications. Transfusion reactions. 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A ; WILPSHAAR, J ; HERTOGH, C. D. P ; RAD, M ; LURVINK, E. G. A ; VAN LUXEMBURG-HEIJS, S. A. P ; ZWINDERMAN, K ; VERWEY, R. A ; WILLEMZE, R ; FALKENBURG, J. H. F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-73db22cc5e9884ddd28e8d6b5a9e621aa9ee8cf66e259fd33d362fb717034343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adult</topic><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antigens, CD - blood</topic><topic>Antigens, CD34 - blood</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - immunology</topic><topic>Bone marrow transplantation</topic><topic>Bone marrow, stem cells transplantation. 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A</au><au>WILPSHAAR, J</au><au>HERTOGH, C. D. P</au><au>RAD, M</au><au>LURVINK, E. G. A</au><au>VAN LUXEMBURG-HEIJS, S. A. P</au><au>ZWINDERMAN, K</au><au>VERWEY, R. A</au><au>WILLEMZE, R</au><au>FALKENBURG, J. H. F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Similar myeloid recovery despite superior overall engraftment in NOD/SCID mice after transplantation of human CD34+ cells from umbilical cord blood as compared to adult sources</atitle><jtitle>Bone marrow transplantation (Basingstoke)</jtitle><addtitle>Bone Marrow Transplant</addtitle><date>2001-07-01</date><risdate>2001</risdate><volume>28</volume><issue>2</issue><spage>163</spage><epage>171</epage><pages>163-171</pages><issn>0268-3369</issn><eissn>1476-5365</eissn><coden>BMTRE9</coden><abstract>Umbilical cord blood (UCB), bone marrow (BM) and mobilized peripheral blood (mPB) are used as sources of hematopoietic stem cells for transplantation. The NOD/SCID mouse model was used to compare the lineage-specific repopulating potential of CD34(+) cells derived from these sources. Six to 8 weeks after transplantation, blood, BM, spleen, liver and thymus, were harvested, and analyzed by flow cytometry using CD34, CD45, myeloid, and lymphoid lineage-specific antibodies. Fifty percent engraftment of human cells in bone marrow of mice was estimated to be reached with 0.55 x 10(6) CD34(+) UCB cells or with 7.9 x 10(6) CD34(+) cells from adult sources, illustrating a 10-fold superiority of UCB CD34(+) cells to engraft NOD/SCID mice. Lineage-specific characterization of engrafted human cells showed that the high engraftment potential of CD34(+) cells from UCB was due to a preferential B cell development (2-81%). In contrast, comparable percentages of myeloid cells were found following transplantation of CD34(+) cells from UCB, BM and mPB (1-38%), and occurred at significant levels only at relatively high doses. Since the CD34 content of UCB transplants is usually at least one log lower than of transplant from adult sources, these results correspond to the clinical findings with UCB transplantation showing a relatively high overall engraftment, but delayed myeloid recovery.</abstract><cop>Basingstoke</cop><pub>Nature Publishing Group</pub><pmid>11509934</pmid><doi>10.1038/sj.bmt.1703120</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0268-3369 |
| ispartof | Bone marrow transplantation (Basingstoke), 2001-07, Vol.28 (2), p.163-171 |
| issn | 0268-3369 1476-5365 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adult Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Antibodies Antigens, CD - blood Antigens, CD34 - blood Biological and medical sciences Blood Bone marrow Bone Marrow Cells - cytology Bone Marrow Cells - immunology Bone marrow transplantation Bone marrow, stem cells transplantation. Graft versus host reaction CD34 antigen CD45 antigen Cord blood Engraftment Fetal Blood - cytology Flow Cytometry Hematopoietic Stem Cell Transplantation Hematopoietic stem cells Humans Infant, Newborn Liver - cytology Liver - immunology Medical sciences Mice Mice, Inbred NOD Mice, SCID Myeloid cells Peripheral blood Recovery Spleen Spleen - cytology Spleen - immunology Stem cell transplantation Stem cells Thymus Gland - cytology Thymus Gland - immunology Transfusions. Complications. Transfusion reactions. Cell and gene therapy Transplantation Transplantation, Heterologous - physiology Transplants Transplants & implants Umbilical cord |
| title | Similar myeloid recovery despite superior overall engraftment in NOD/SCID mice after transplantation of human CD34+ cells from umbilical cord blood as compared to adult sources |
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