Localization of Cytosolic NADP-dependent Isocitrate Dehydrogenase in the Peroxisomes of Rat Liver Cells: Biochemical and Immunocytochemical Studies

Two types of NADP-dependent isocitrate dehydrogenases (ICDs) have been reported: mitochondrial (ICD1) and cytosolic (ICD2). The C-terminal amino acid sequence of ICD2 has a tripeptide peroxisome targeting signal 1 sequence (PTS1). After differential centrifugation of the postnuclear fraction of rat...

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Veröffentlicht in:	The journal of histochemistry and cytochemistry 2001-09, Vol.49 (9), p.1123-1131
Hauptverfasser:	Yoshihara, Takashi, Hamamoto, Tatsuhiko, Munakata, Ryo, Tajiri, Ryosuke, Ohsumi, Mariko, Yokota, Sadaki
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibody Specificity Catalase - metabolism Cell Fractionation Centrifugation, Density Gradient Cytosol - enzymology Fluorescent Antibody Technique Frozen Sections Immunoblotting Immunohistochemistry Isocitrate Dehydrogenase - immunology Isocitrate Dehydrogenase - metabolism Liver - enzymology Liver - ultrastructure Male Microscopy, Electron - methods NADP - metabolism Organelles - enzymology Peroxisomes - enzymology Rats Rats, Wistar
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