Immunohistochemical examination of the lungs in infant death cases using antibodies against milk components

To examine the use of immunohistochemical staining with antibodies against milk components for detection of aspirated milk on lung sections, eighteen infant death cases were investigated. Immunostaining was performed with anti-human α lactalbumin, anti-human IgA, anti-human milk fat globulin 1, and...

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Veröffentlicht in:Forensic science international 2000-05, Vol.110 (1), p.19-28
Hauptverfasser: Iwadate, Kimiharu, Doy, Mikio, Nishimaki, Yuko, Liang, Fang, Takatori, Takehiko, Hasekura, Hayato
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container_title Forensic science international
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creator Iwadate, Kimiharu
Doy, Mikio
Nishimaki, Yuko
Liang, Fang
Takatori, Takehiko
Hasekura, Hayato
description To examine the use of immunohistochemical staining with antibodies against milk components for detection of aspirated milk on lung sections, eighteen infant death cases were investigated. Immunostaining was performed with anti-human α lactalbumin, anti-human IgA, anti-human milk fat globulin 1, and anti-cow whey antibody. Reactivity with each antibody was examined, and semi-quantitative examinations were performed to compare the amount of aspirated milk using anti-human α lactalbumin antibody. Materials in the alveoli or bronchioli on lung sections suspected to be aspirated milk showed the most sensitive and clearest reaction with anti-human α lactalbumin antibody. Of the eighteen cases, ten cases showed positive reaction with this antibody. The amount of aspirated milk varied widely in each case. In conclusion, immunohistochemical staining with antibodies against human milk components, especially anti-human α lactalbumin antibody, can detect small amounts of milk. Using this method, we were able to compare the relative amount of aspirated milk among cases.
doi_str_mv 10.1016/S0379-0738(99)00229-7
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Immunostaining was performed with anti-human α lactalbumin, anti-human IgA, anti-human milk fat globulin 1, and anti-cow whey antibody. Reactivity with each antibody was examined, and semi-quantitative examinations were performed to compare the amount of aspirated milk using anti-human α lactalbumin antibody. Materials in the alveoli or bronchioli on lung sections suspected to be aspirated milk showed the most sensitive and clearest reaction with anti-human α lactalbumin antibody. Of the eighteen cases, ten cases showed positive reaction with this antibody. The amount of aspirated milk varied widely in each case. In conclusion, immunohistochemical staining with antibodies against human milk components, especially anti-human α lactalbumin antibody, can detect small amounts of milk. Using this method, we were able to compare the relative amount of aspirated milk among cases.</description><identifier>ISSN: 0379-0738</identifier><identifier>EISSN: 1872-6283</identifier><identifier>DOI: 10.1016/S0379-0738(99)00229-7</identifier><identifier>PMID: 10802197</identifier><identifier>CODEN: FSINDR</identifier><language>eng</language><publisher>Kidlington: Elsevier Ireland Ltd</publisher><subject>Alveoli ; Analysis of Variance ; Animal fat ; Animals ; Antibodies ; Autopsies ; Babies ; Biological and medical sciences ; Breast milk ; Bronchi - pathology ; Cause of Death ; Coloring Agents ; Delivery. Postpartum. Lactation ; Disorders ; Female ; Forensic pathology ; Globulins ; Glycolipids - analysis ; Glycoproteins - analysis ; Gynecology. Andrology. Obstetrics ; Humans ; Immunoglobulin A ; Immunoglobulin A, Secretory - analysis ; Immunohistochemistry ; Infant ; Infant mortality ; Infant, Newborn ; Infants ; Lactalbumin ; Lactalbumin - analysis ; Lung - immunology ; Lung - pathology ; Lungs ; Male ; Medical sciences ; Milk ; Milk - chemistry ; Milk - immunology ; Milk aspiration ; Milk Proteins - analysis ; Milk, Human - chemistry ; Milk, Human - immunology ; Oils &amp; fats ; Pneumonia, Aspiration - diagnosis ; Pneumonia, Aspiration - immunology ; Pneumonia, Aspiration - pathology ; Pulmonary Alveoli - pathology ; Sensitivity and Specificity ; Staining ; Statistics, Nonparametric ; Sudden infant death ; Whey Proteins</subject><ispartof>Forensic science international, 2000-05, Vol.110 (1), p.19-28</ispartof><rights>2000 Elsevier Science Ireland Ltd</rights><rights>2000 INIST-CNRS</rights><rights>2000. 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Immunostaining was performed with anti-human α lactalbumin, anti-human IgA, anti-human milk fat globulin 1, and anti-cow whey antibody. Reactivity with each antibody was examined, and semi-quantitative examinations were performed to compare the amount of aspirated milk using anti-human α lactalbumin antibody. Materials in the alveoli or bronchioli on lung sections suspected to be aspirated milk showed the most sensitive and clearest reaction with anti-human α lactalbumin antibody. Of the eighteen cases, ten cases showed positive reaction with this antibody. The amount of aspirated milk varied widely in each case. In conclusion, immunohistochemical staining with antibodies against human milk components, especially anti-human α lactalbumin antibody, can detect small amounts of milk. Using this method, we were able to compare the relative amount of aspirated milk among cases.</description><subject>Alveoli</subject><subject>Analysis of Variance</subject><subject>Animal fat</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Autopsies</subject><subject>Babies</subject><subject>Biological and medical sciences</subject><subject>Breast milk</subject><subject>Bronchi - pathology</subject><subject>Cause of Death</subject><subject>Coloring Agents</subject><subject>Delivery. Postpartum. Lactation</subject><subject>Disorders</subject><subject>Female</subject><subject>Forensic pathology</subject><subject>Globulins</subject><subject>Glycolipids - analysis</subject><subject>Glycoproteins - analysis</subject><subject>Gynecology. Andrology. 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Immunostaining was performed with anti-human α lactalbumin, anti-human IgA, anti-human milk fat globulin 1, and anti-cow whey antibody. Reactivity with each antibody was examined, and semi-quantitative examinations were performed to compare the amount of aspirated milk using anti-human α lactalbumin antibody. Materials in the alveoli or bronchioli on lung sections suspected to be aspirated milk showed the most sensitive and clearest reaction with anti-human α lactalbumin antibody. Of the eighteen cases, ten cases showed positive reaction with this antibody. The amount of aspirated milk varied widely in each case. In conclusion, immunohistochemical staining with antibodies against human milk components, especially anti-human α lactalbumin antibody, can detect small amounts of milk. Using this method, we were able to compare the relative amount of aspirated milk among cases.</abstract><cop>Kidlington</cop><pub>Elsevier Ireland Ltd</pub><pmid>10802197</pmid><doi>10.1016/S0379-0738(99)00229-7</doi><tpages>10</tpages></addata></record>
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subjects Alveoli
Analysis of Variance
Animal fat
Animals
Antibodies
Autopsies
Babies
Biological and medical sciences
Breast milk
Bronchi - pathology
Cause of Death
Coloring Agents
Delivery. Postpartum. Lactation
Disorders
Female
Forensic pathology
Globulins
Glycolipids - analysis
Glycoproteins - analysis
Gynecology. Andrology. Obstetrics
Humans
Immunoglobulin A
Immunoglobulin A, Secretory - analysis
Immunohistochemistry
Infant
Infant mortality
Infant, Newborn
Infants
Lactalbumin
Lactalbumin - analysis
Lung - immunology
Lung - pathology
Lungs
Male
Medical sciences
Milk
Milk - chemistry
Milk - immunology
Milk aspiration
Milk Proteins - analysis
Milk, Human - chemistry
Milk, Human - immunology
Oils & fats
Pneumonia, Aspiration - diagnosis
Pneumonia, Aspiration - immunology
Pneumonia, Aspiration - pathology
Pulmonary Alveoli - pathology
Sensitivity and Specificity
Staining
Statistics, Nonparametric
Sudden infant death
Whey Proteins
title Immunohistochemical examination of the lungs in infant death cases using antibodies against milk components
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