Immunohistochemical examination of the lungs in infant death cases using antibodies against milk components
To examine the use of immunohistochemical staining with antibodies against milk components for detection of aspirated milk on lung sections, eighteen infant death cases were investigated. Immunostaining was performed with anti-human α lactalbumin, anti-human IgA, anti-human milk fat globulin 1, and...
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description | To examine the use of immunohistochemical staining with antibodies against milk components for detection of aspirated milk on lung sections, eighteen infant death cases were investigated. Immunostaining was performed with anti-human α lactalbumin, anti-human IgA, anti-human milk fat globulin 1, and anti-cow whey antibody. Reactivity with each antibody was examined, and semi-quantitative examinations were performed to compare the amount of aspirated milk using anti-human α lactalbumin antibody. Materials in the alveoli or bronchioli on lung sections suspected to be aspirated milk showed the most sensitive and clearest reaction with anti-human α lactalbumin antibody. Of the eighteen cases, ten cases showed positive reaction with this antibody. The amount of aspirated milk varied widely in each case. In conclusion, immunohistochemical staining with antibodies against human milk components, especially anti-human α lactalbumin antibody, can detect small amounts of milk. Using this method, we were able to compare the relative amount of aspirated milk among cases. |
doi_str_mv | 10.1016/S0379-0738(99)00229-7 |
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Immunostaining was performed with anti-human α lactalbumin, anti-human IgA, anti-human milk fat globulin 1, and anti-cow whey antibody. Reactivity with each antibody was examined, and semi-quantitative examinations were performed to compare the amount of aspirated milk using anti-human α lactalbumin antibody. Materials in the alveoli or bronchioli on lung sections suspected to be aspirated milk showed the most sensitive and clearest reaction with anti-human α lactalbumin antibody. Of the eighteen cases, ten cases showed positive reaction with this antibody. The amount of aspirated milk varied widely in each case. In conclusion, immunohistochemical staining with antibodies against human milk components, especially anti-human α lactalbumin antibody, can detect small amounts of milk. Using this method, we were able to compare the relative amount of aspirated milk among cases.</description><identifier>ISSN: 0379-0738</identifier><identifier>EISSN: 1872-6283</identifier><identifier>DOI: 10.1016/S0379-0738(99)00229-7</identifier><identifier>PMID: 10802197</identifier><identifier>CODEN: FSINDR</identifier><language>eng</language><publisher>Kidlington: Elsevier Ireland Ltd</publisher><subject>Alveoli ; Analysis of Variance ; Animal fat ; Animals ; Antibodies ; Autopsies ; Babies ; Biological and medical sciences ; Breast milk ; Bronchi - pathology ; Cause of Death ; Coloring Agents ; Delivery. Postpartum. Lactation ; Disorders ; Female ; Forensic pathology ; Globulins ; Glycolipids - analysis ; Glycoproteins - analysis ; Gynecology. Andrology. Obstetrics ; Humans ; Immunoglobulin A ; Immunoglobulin A, Secretory - analysis ; Immunohistochemistry ; Infant ; Infant mortality ; Infant, Newborn ; Infants ; Lactalbumin ; Lactalbumin - analysis ; Lung - immunology ; Lung - pathology ; Lungs ; Male ; Medical sciences ; Milk ; Milk - chemistry ; Milk - immunology ; Milk aspiration ; Milk Proteins - analysis ; Milk, Human - chemistry ; Milk, Human - immunology ; Oils & fats ; Pneumonia, Aspiration - diagnosis ; Pneumonia, Aspiration - immunology ; Pneumonia, Aspiration - pathology ; Pulmonary Alveoli - pathology ; Sensitivity and Specificity ; Staining ; Statistics, Nonparametric ; Sudden infant death ; Whey Proteins</subject><ispartof>Forensic science international, 2000-05, Vol.110 (1), p.19-28</ispartof><rights>2000 Elsevier Science Ireland Ltd</rights><rights>2000 INIST-CNRS</rights><rights>2000. 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Immunostaining was performed with anti-human α lactalbumin, anti-human IgA, anti-human milk fat globulin 1, and anti-cow whey antibody. Reactivity with each antibody was examined, and semi-quantitative examinations were performed to compare the amount of aspirated milk using anti-human α lactalbumin antibody. Materials in the alveoli or bronchioli on lung sections suspected to be aspirated milk showed the most sensitive and clearest reaction with anti-human α lactalbumin antibody. Of the eighteen cases, ten cases showed positive reaction with this antibody. The amount of aspirated milk varied widely in each case. In conclusion, immunohistochemical staining with antibodies against human milk components, especially anti-human α lactalbumin antibody, can detect small amounts of milk. Using this method, we were able to compare the relative amount of aspirated milk among cases.</description><subject>Alveoli</subject><subject>Analysis of Variance</subject><subject>Animal fat</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Autopsies</subject><subject>Babies</subject><subject>Biological and medical sciences</subject><subject>Breast milk</subject><subject>Bronchi - pathology</subject><subject>Cause of Death</subject><subject>Coloring Agents</subject><subject>Delivery. Postpartum. Lactation</subject><subject>Disorders</subject><subject>Female</subject><subject>Forensic pathology</subject><subject>Globulins</subject><subject>Glycolipids - analysis</subject><subject>Glycoproteins - analysis</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Immunoglobulin A</subject><subject>Immunoglobulin A, Secretory - analysis</subject><subject>Immunohistochemistry</subject><subject>Infant</subject><subject>Infant mortality</subject><subject>Infant, Newborn</subject><subject>Infants</subject><subject>Lactalbumin</subject><subject>Lactalbumin - analysis</subject><subject>Lung - immunology</subject><subject>Lung - pathology</subject><subject>Lungs</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Milk</subject><subject>Milk - chemistry</subject><subject>Milk - immunology</subject><subject>Milk aspiration</subject><subject>Milk Proteins - analysis</subject><subject>Milk, Human - chemistry</subject><subject>Milk, Human - immunology</subject><subject>Oils & fats</subject><subject>Pneumonia, Aspiration - diagnosis</subject><subject>Pneumonia, Aspiration - immunology</subject><subject>Pneumonia, Aspiration - pathology</subject><subject>Pulmonary Alveoli - pathology</subject><subject>Sensitivity and Specificity</subject><subject>Staining</subject><subject>Statistics, Nonparametric</subject><subject>Sudden infant death</subject><subject>Whey Proteins</subject><issn>0379-0738</issn><issn>1872-6283</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkVuLFDEQhYMo7rj6E5SAIvrQmkunkzwtsnhZWPBBfQ7pdPVMdjvJmKRF_70ZZ1DxRSiooviqOJyD0GNKXlFCh9efCJe6I5KrF1q_JIQx3ck7aEOVZN3AFL-LNr-RM_SglBtCiBBsuI_OKFGEUS036PYqhDWmnS81uR0E7-yC4bsNPtrqU8RpxnUHeFnjtmAfW802VjyBrTvsbIGC1-LjFretH9Pk28JurY-l4uCXW-xS2KcIsZaH6N5slwKPTv0cfXn39vPlh-764_uryzfXneupqt00SDlTJlmvR92rfuRCsZFxxpxtMwihnHRcjhNjYu4HpvvZgZODkJPlrOfn6Pnx7z6nryuUaoIvDpbFRkhrMZISrZXmDXz6D3iT1hybNsN0c0srKmmjxJFyOZWSYTb77IPNPwwl5pCF-ZWFORhtdOuHLIxsd09O39cxwPTX1dH8Bjw7AbY02-dso_PlD9dTMYiDyosjBs2zbx6yKc5DdDD5DK6aKfn_KPkJJ3umEw</recordid><startdate>20000508</startdate><enddate>20000508</enddate><creator>Iwadate, Kimiharu</creator><creator>Doy, Mikio</creator><creator>Nishimaki, Yuko</creator><creator>Liang, Fang</creator><creator>Takatori, Takehiko</creator><creator>Hasekura, Hayato</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20000508</creationdate><title>Immunohistochemical examination of the lungs in infant death cases using antibodies against milk components</title><author>Iwadate, Kimiharu ; Doy, Mikio ; Nishimaki, Yuko ; Liang, Fang ; Takatori, Takehiko ; Hasekura, Hayato</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-d677f127249b9484b3582b2322cab35e558c7c37bd225f46294fcec7657da3243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Alveoli</topic><topic>Analysis of Variance</topic><topic>Animal fat</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Autopsies</topic><topic>Babies</topic><topic>Biological and medical sciences</topic><topic>Breast milk</topic><topic>Bronchi - pathology</topic><topic>Cause of Death</topic><topic>Coloring Agents</topic><topic>Delivery. Postpartum. Lactation</topic><topic>Disorders</topic><topic>Female</topic><topic>Forensic pathology</topic><topic>Globulins</topic><topic>Glycolipids - analysis</topic><topic>Glycoproteins - analysis</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Immunoglobulin A</topic><topic>Immunoglobulin A, Secretory - analysis</topic><topic>Immunohistochemistry</topic><topic>Infant</topic><topic>Infant mortality</topic><topic>Infant, Newborn</topic><topic>Infants</topic><topic>Lactalbumin</topic><topic>Lactalbumin - analysis</topic><topic>Lung - immunology</topic><topic>Lung - pathology</topic><topic>Lungs</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Milk</topic><topic>Milk - chemistry</topic><topic>Milk - immunology</topic><topic>Milk aspiration</topic><topic>Milk Proteins - analysis</topic><topic>Milk, Human - chemistry</topic><topic>Milk, Human - immunology</topic><topic>Oils & fats</topic><topic>Pneumonia, Aspiration - diagnosis</topic><topic>Pneumonia, Aspiration - immunology</topic><topic>Pneumonia, Aspiration - pathology</topic><topic>Pulmonary Alveoli - pathology</topic><topic>Sensitivity and Specificity</topic><topic>Staining</topic><topic>Statistics, Nonparametric</topic><topic>Sudden infant death</topic><topic>Whey Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Iwadate, Kimiharu</creatorcontrib><creatorcontrib>Doy, Mikio</creatorcontrib><creatorcontrib>Nishimaki, Yuko</creatorcontrib><creatorcontrib>Liang, Fang</creatorcontrib><creatorcontrib>Takatori, Takehiko</creatorcontrib><creatorcontrib>Hasekura, Hayato</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Toxicology Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest research library</collection><collection>ProQuest Biological Science Journals</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Forensic science international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Iwadate, Kimiharu</au><au>Doy, Mikio</au><au>Nishimaki, Yuko</au><au>Liang, Fang</au><au>Takatori, Takehiko</au><au>Hasekura, Hayato</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical examination of the lungs in infant death cases using antibodies against milk components</atitle><jtitle>Forensic science international</jtitle><addtitle>Forensic Sci Int</addtitle><date>2000-05-08</date><risdate>2000</risdate><volume>110</volume><issue>1</issue><spage>19</spage><epage>28</epage><pages>19-28</pages><issn>0379-0738</issn><eissn>1872-6283</eissn><coden>FSINDR</coden><abstract>To examine the use of immunohistochemical staining with antibodies against milk components for detection of aspirated milk on lung sections, eighteen infant death cases were investigated. Immunostaining was performed with anti-human α lactalbumin, anti-human IgA, anti-human milk fat globulin 1, and anti-cow whey antibody. Reactivity with each antibody was examined, and semi-quantitative examinations were performed to compare the amount of aspirated milk using anti-human α lactalbumin antibody. Materials in the alveoli or bronchioli on lung sections suspected to be aspirated milk showed the most sensitive and clearest reaction with anti-human α lactalbumin antibody. Of the eighteen cases, ten cases showed positive reaction with this antibody. The amount of aspirated milk varied widely in each case. In conclusion, immunohistochemical staining with antibodies against human milk components, especially anti-human α lactalbumin antibody, can detect small amounts of milk. Using this method, we were able to compare the relative amount of aspirated milk among cases.</abstract><cop>Kidlington</cop><pub>Elsevier Ireland Ltd</pub><pmid>10802197</pmid><doi>10.1016/S0379-0738(99)00229-7</doi><tpages>10</tpages></addata></record> |
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subjects | Alveoli Analysis of Variance Animal fat Animals Antibodies Autopsies Babies Biological and medical sciences Breast milk Bronchi - pathology Cause of Death Coloring Agents Delivery. Postpartum. Lactation Disorders Female Forensic pathology Globulins Glycolipids - analysis Glycoproteins - analysis Gynecology. Andrology. Obstetrics Humans Immunoglobulin A Immunoglobulin A, Secretory - analysis Immunohistochemistry Infant Infant mortality Infant, Newborn Infants Lactalbumin Lactalbumin - analysis Lung - immunology Lung - pathology Lungs Male Medical sciences Milk Milk - chemistry Milk - immunology Milk aspiration Milk Proteins - analysis Milk, Human - chemistry Milk, Human - immunology Oils & fats Pneumonia, Aspiration - diagnosis Pneumonia, Aspiration - immunology Pneumonia, Aspiration - pathology Pulmonary Alveoli - pathology Sensitivity and Specificity Staining Statistics, Nonparametric Sudden infant death Whey Proteins |
title | Immunohistochemical examination of the lungs in infant death cases using antibodies against milk components |
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