cDNA cloning of phosphoethanolamine N-methyltransferase from spinach by complementation in Schizosaccharomyces pombe and characterization of the recombinant enzyme

The N-methylation of phosphoethanolamine is the committing step in choline biogenesis in plants and is catalyzed by S-adenosyl-L-methionine:phosphoethanolamine N-methyltransferase (PEAMT, EC ). A spinach PEAMT cDNA was isolated by functional complementation of a Schizosaccharomyces pombe cho2(-) mut...

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Veröffentlicht in:	The Journal of biological chemistry 2000-05, Vol.275 (19), p.14095-14101
Hauptverfasser:	Nuccio, M.L, Ziemak, M.J, Henry, S.A, Weretilnyk, E.A, Hanson, A.D
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences Base Sequence Catalysis choline cloning Cloning, Molecular complementary DNA derivatives DNA, Complementary enzyme activity genbank/af237633 gene expression genetic complementation Genetic Complementation Test messenger RNA Methyltransferases - genetics Methyltransferases - metabolism Molecular Sequence Data mutants nucleotide sequences phosphatidylethanolamines phosphocholine phosphoethanolamine Phosphoethanolamine N-methyltransferase recombinant proteins Recombinant Proteins - genetics Recombinant Proteins - metabolism salinity Salts Schizosaccharomyces - genetics Schizosaccharomyces pombe Sequence Homology, Amino Acid Spinacea oleracea Spinacia oleracea Spinacia oleracea - enzymology Spinacia oleracea - genetics transferases
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