MYCOPLASMOSIS IN CAPTIVE CROWS AND ROBINS FROM MINNESOTA
Mycoplasma sturni is a recently described organism previously associated with conjunctivitis in European starlings (Sturnus vulgaris), northern mockingbirds (Mimus polyglottos) and blue jays (Cyanocitta cristata). Herein we describe the isolation of M. sturni from an American crow (Corvus brachyrhyn...
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| Veröffentlicht in: | Journal of wildlife diseases 2001-07, Vol.37 (3), p.547-555 |
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| container_title | Journal of wildlife diseases |
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| creator | Wellehan, James F. X. Calsamiglia, Maria Ley, David H. Zens, Mark S. Amonsin, Alongkorn Kapur, Vivek |
| description | Mycoplasma sturni is a recently described organism previously associated with conjunctivitis in European starlings (Sturnus vulgaris), northern mockingbirds (Mimus polyglottos) and blue jays (Cyanocitta cristata). Herein we describe the isolation of M. sturni from an American crow (Corvus brachyrhynchos) presenting with conjunctivitis. A nested-PCR was designed for identification of M. sturni in clinical specimens and the sensitivity of the reaction was found to be 10 colony-changing units. The organism was found in asymptomatic American crows caged with a nestmate of the crow with conjunctivitis. Mycoplasma sturni also was found in asymptomatic American robins (Turdus migratorius) and in a European starling (Sturnus vulgaris) housed at the same facility as the crows. Heterogenity of M. sturni isolates from different host species was found by random amplified polymorphic DNA (RAPD) analyses. Heterogeneity also was found among M. sturni isolates recovered from American crows. We suggest that M. sturni can successfully infect American crows and American robins with or without the presence of clinical disease. Furthermore, we demonstrate that nested-PCR is an effective method for the detection of M. sturni and that substantial genetic heterogeneity exists among natural isolates of this bacterial pathogen. |
| doi_str_mv | 10.7589/0090-3558-37.3.547 |
| format | Article |
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X. ; Calsamiglia, Maria ; Ley, David H. ; Zens, Mark S. ; Amonsin, Alongkorn ; Kapur, Vivek</creator><creatorcontrib>Wellehan, James F. X. ; Calsamiglia, Maria ; Ley, David H. ; Zens, Mark S. ; Amonsin, Alongkorn ; Kapur, Vivek</creatorcontrib><description>Mycoplasma sturni is a recently described organism previously associated with conjunctivitis in European starlings (Sturnus vulgaris), northern mockingbirds (Mimus polyglottos) and blue jays (Cyanocitta cristata). Herein we describe the isolation of M. sturni from an American crow (Corvus brachyrhynchos) presenting with conjunctivitis. A nested-PCR was designed for identification of M. sturni in clinical specimens and the sensitivity of the reaction was found to be 10 colony-changing units. The organism was found in asymptomatic American crows caged with a nestmate of the crow with conjunctivitis. Mycoplasma sturni also was found in asymptomatic American robins (Turdus migratorius) and in a European starling (Sturnus vulgaris) housed at the same facility as the crows. Heterogenity of M. sturni isolates from different host species was found by random amplified polymorphic DNA (RAPD) analyses. Heterogeneity also was found among M. sturni isolates recovered from American crows. We suggest that M. sturni can successfully infect American crows and American robins with or without the presence of clinical disease. Furthermore, we demonstrate that nested-PCR is an effective method for the detection of M. sturni and that substantial genetic heterogeneity exists among natural isolates of this bacterial pathogen.</description><identifier>ISSN: 0090-3558</identifier><identifier>EISSN: 1943-3700</identifier><identifier>DOI: 10.7589/0090-3558-37.3.547</identifier><identifier>PMID: 11504228</identifier><language>eng</language><publisher>United States: Wildlife Disease Association</publisher><subject>American crow ; American robin ; Animals ; Base Sequence ; Bird Diseases - epidemiology ; Bird Diseases - microbiology ; Colony Count, Microbial ; Conjunctivitis, Bacterial - epidemiology ; Conjunctivitis, Bacterial - microbiology ; Conjunctivitis, Bacterial - veterinary ; Corvus brachyrhynchos ; DNA Fingerprinting - veterinary ; DNA, Ribosomal - chemistry ; Fluorescent Antibody Technique, Indirect ; Minnesota - epidemiology ; Mycoplasma - classification ; Mycoplasma - genetics ; Mycoplasma - isolation & purification ; Mycoplasma Infections - epidemiology ; Mycoplasma Infections - veterinary ; Mycoplasma sturni ; mycoplasmosis ; nested polymerase chain reaction ; Phylogeny ; Random Amplified Polymorphic DNA Technique - methods ; Random Amplified Polymorphic DNA Technique - veterinary ; RNA, Ribosomal, 16S - genetics ; Sensitivity and Specificity ; Songbirds ; Turdus migratorius</subject><ispartof>Journal of wildlife diseases, 2001-07, Vol.37 (3), p.547-555</ispartof><rights>Wildlife Disease Association 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b445t-cc20f5bdca1f6959fd5bb52d2804c57cc6a1511fe461995668f8bec531286b153</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.7589/0090-3558-37.3.547$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>109,314,780,784,27924,27925,52719</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11504228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wellehan, James F. X.</creatorcontrib><creatorcontrib>Calsamiglia, Maria</creatorcontrib><creatorcontrib>Ley, David H.</creatorcontrib><creatorcontrib>Zens, Mark S.</creatorcontrib><creatorcontrib>Amonsin, Alongkorn</creatorcontrib><creatorcontrib>Kapur, Vivek</creatorcontrib><title>MYCOPLASMOSIS IN CAPTIVE CROWS AND ROBINS FROM MINNESOTA</title><title>Journal of wildlife diseases</title><addtitle>J Wildl Dis</addtitle><description>Mycoplasma sturni is a recently described organism previously associated with conjunctivitis in European starlings (Sturnus vulgaris), northern mockingbirds (Mimus polyglottos) and blue jays (Cyanocitta cristata). Herein we describe the isolation of M. sturni from an American crow (Corvus brachyrhynchos) presenting with conjunctivitis. A nested-PCR was designed for identification of M. sturni in clinical specimens and the sensitivity of the reaction was found to be 10 colony-changing units. The organism was found in asymptomatic American crows caged with a nestmate of the crow with conjunctivitis. Mycoplasma sturni also was found in asymptomatic American robins (Turdus migratorius) and in a European starling (Sturnus vulgaris) housed at the same facility as the crows. Heterogenity of M. sturni isolates from different host species was found by random amplified polymorphic DNA (RAPD) analyses. Heterogeneity also was found among M. sturni isolates recovered from American crows. We suggest that M. sturni can successfully infect American crows and American robins with or without the presence of clinical disease. Furthermore, we demonstrate that nested-PCR is an effective method for the detection of M. sturni and that substantial genetic heterogeneity exists among natural isolates of this bacterial pathogen.</description><subject>American crow</subject><subject>American robin</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Bird Diseases - epidemiology</subject><subject>Bird Diseases - microbiology</subject><subject>Colony Count, Microbial</subject><subject>Conjunctivitis, Bacterial - epidemiology</subject><subject>Conjunctivitis, Bacterial - microbiology</subject><subject>Conjunctivitis, Bacterial - veterinary</subject><subject>Corvus brachyrhynchos</subject><subject>DNA Fingerprinting - veterinary</subject><subject>DNA, Ribosomal - chemistry</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Minnesota - epidemiology</subject><subject>Mycoplasma - classification</subject><subject>Mycoplasma - genetics</subject><subject>Mycoplasma - isolation & purification</subject><subject>Mycoplasma Infections - epidemiology</subject><subject>Mycoplasma Infections - veterinary</subject><subject>Mycoplasma sturni</subject><subject>mycoplasmosis</subject><subject>nested polymerase chain reaction</subject><subject>Phylogeny</subject><subject>Random Amplified Polymorphic DNA Technique - methods</subject><subject>Random Amplified Polymorphic DNA Technique - veterinary</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sensitivity and Specificity</subject><subject>Songbirds</subject><subject>Turdus migratorius</subject><issn>0090-3558</issn><issn>1943-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEFPgzAYhhujcXP6BzwYLnpjtqWF9oi4KcmAZUyNpwZKcRgYk24h_ntZWNSbnr68yfO-X_IAcIng2KGM30LIoWlRykzLGVtjSpwjMEScWF2G8BgMv4EBONP6HUJMu3AKBghRSDBmQ8CCVy-az9w4iGI_NvzQ8Nz50n-eGN4ieokNN7w3FtGdH8bGdBEFRuCH4SSOlu45OMmTUquLwx2Bp-lk6T2as-jB99yZmRJCt6aUGOY0zWSCcptTnmc0TSnOMINEUkdKO0EUoVwRG3FObZvlLFWSWggzO0XUGoGbfnfT1B87pbeiKrRUZZmsVb3TwkGQM8bwnyBimCACWQfiHpRNrXWjcrFpiippPgWCYi9W7L2JvTdhOcISndiudHVY36WVyn4qB5MdcN0Dq-Jt1RaNErpKyrLDsWjb9tcQ7Lm0qOu1-s_vLzDyiGo</recordid><startdate>20010701</startdate><enddate>20010701</enddate><creator>Wellehan, James F. X.</creator><creator>Calsamiglia, Maria</creator><creator>Ley, David H.</creator><creator>Zens, Mark S.</creator><creator>Amonsin, Alongkorn</creator><creator>Kapur, Vivek</creator><general>Wildlife Disease Association</general><general>Wildlife Dis Assoc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20010701</creationdate><title>MYCOPLASMOSIS IN CAPTIVE CROWS AND ROBINS FROM MINNESOTA</title><author>Wellehan, James F. X. ; Calsamiglia, Maria ; Ley, David H. ; Zens, Mark S. ; Amonsin, Alongkorn ; Kapur, Vivek</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b445t-cc20f5bdca1f6959fd5bb52d2804c57cc6a1511fe461995668f8bec531286b153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>American crow</topic><topic>American robin</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Bird Diseases - epidemiology</topic><topic>Bird Diseases - microbiology</topic><topic>Colony Count, Microbial</topic><topic>Conjunctivitis, Bacterial - epidemiology</topic><topic>Conjunctivitis, Bacterial - microbiology</topic><topic>Conjunctivitis, Bacterial - veterinary</topic><topic>Corvus brachyrhynchos</topic><topic>DNA Fingerprinting - veterinary</topic><topic>DNA, Ribosomal - chemistry</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Minnesota - epidemiology</topic><topic>Mycoplasma - classification</topic><topic>Mycoplasma - genetics</topic><topic>Mycoplasma - isolation & purification</topic><topic>Mycoplasma Infections - epidemiology</topic><topic>Mycoplasma Infections - veterinary</topic><topic>Mycoplasma sturni</topic><topic>mycoplasmosis</topic><topic>nested polymerase chain reaction</topic><topic>Phylogeny</topic><topic>Random Amplified Polymorphic DNA Technique - methods</topic><topic>Random Amplified Polymorphic DNA Technique - veterinary</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sensitivity and Specificity</topic><topic>Songbirds</topic><topic>Turdus migratorius</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wellehan, James F. X.</creatorcontrib><creatorcontrib>Calsamiglia, Maria</creatorcontrib><creatorcontrib>Ley, David H.</creatorcontrib><creatorcontrib>Zens, Mark S.</creatorcontrib><creatorcontrib>Amonsin, Alongkorn</creatorcontrib><creatorcontrib>Kapur, Vivek</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of wildlife diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wellehan, James F. X.</au><au>Calsamiglia, Maria</au><au>Ley, David H.</au><au>Zens, Mark S.</au><au>Amonsin, Alongkorn</au><au>Kapur, Vivek</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MYCOPLASMOSIS IN CAPTIVE CROWS AND ROBINS FROM MINNESOTA</atitle><jtitle>Journal of wildlife diseases</jtitle><addtitle>J Wildl Dis</addtitle><date>2001-07-01</date><risdate>2001</risdate><volume>37</volume><issue>3</issue><spage>547</spage><epage>555</epage><pages>547-555</pages><issn>0090-3558</issn><eissn>1943-3700</eissn><abstract>Mycoplasma sturni is a recently described organism previously associated with conjunctivitis in European starlings (Sturnus vulgaris), northern mockingbirds (Mimus polyglottos) and blue jays (Cyanocitta cristata). Herein we describe the isolation of M. sturni from an American crow (Corvus brachyrhynchos) presenting with conjunctivitis. A nested-PCR was designed for identification of M. sturni in clinical specimens and the sensitivity of the reaction was found to be 10 colony-changing units. The organism was found in asymptomatic American crows caged with a nestmate of the crow with conjunctivitis. Mycoplasma sturni also was found in asymptomatic American robins (Turdus migratorius) and in a European starling (Sturnus vulgaris) housed at the same facility as the crows. Heterogenity of M. sturni isolates from different host species was found by random amplified polymorphic DNA (RAPD) analyses. Heterogeneity also was found among M. sturni isolates recovered from American crows. We suggest that M. sturni can successfully infect American crows and American robins with or without the presence of clinical disease. 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| source | MEDLINE; BioOne Open Access Titles; Allen Press Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | American crow American robin Animals Base Sequence Bird Diseases - epidemiology Bird Diseases - microbiology Colony Count, Microbial Conjunctivitis, Bacterial - epidemiology Conjunctivitis, Bacterial - microbiology Conjunctivitis, Bacterial - veterinary Corvus brachyrhynchos DNA Fingerprinting - veterinary DNA, Ribosomal - chemistry Fluorescent Antibody Technique, Indirect Minnesota - epidemiology Mycoplasma - classification Mycoplasma - genetics Mycoplasma - isolation & purification Mycoplasma Infections - epidemiology Mycoplasma Infections - veterinary Mycoplasma sturni mycoplasmosis nested polymerase chain reaction Phylogeny Random Amplified Polymorphic DNA Technique - methods Random Amplified Polymorphic DNA Technique - veterinary RNA, Ribosomal, 16S - genetics Sensitivity and Specificity Songbirds Turdus migratorius |
| title | MYCOPLASMOSIS IN CAPTIVE CROWS AND ROBINS FROM MINNESOTA |
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