Isolation of Antibody to Human Placental Alkaline Phosphatase (PLAP) from Extracts of Human Placentae

PROBLEM: Human placental alkaline phosphatase (PLAP) is a unique placental antigen bound to the syncytiotrophoblast, which may be able to elicit a specific immune response in pregnancy.  METHOD OF STUDY: Antibody to PLAP was purified from placental extracts by: a) acid elution of membrane vesicles;...

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Veröffentlicht in:	American journal of reproductive immunology (1989) 2001-08, Vol.46 (2), p.149-160
Hauptverfasser:	Freeman, Jennifer L., Rhyne, Paul W., Dean, Deyrick O., Beard, G. Alan, Doellgast, George J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase Antibodies - isolation & purification Antibodies, Monoclonal - immunology Biological and medical sciences Chromatography, Affinity - methods Female Fetal antigen Fundamental and applied biological sciences. Psychology GPI-Linked Proteins Hormone metabolism and regulation human placental alkaline phosphatase Humans Hydrochloric Acid - pharmacology Imidazoles - pharmacology Immunoglobulin G - immunology Immunoglobulin G - isolation & purification Immunoglobulin M - isolation & purification Isoenzymes - drug effects Isoenzymes - immunology placenta Placenta - immunology Precipitin Tests - methods Pregnancy Pregnancy. Parturition. Lactation Protein Denaturation Sodium Chloride - pharmacology syncytiotrophoblast Tissue Extracts Vertebrates: reproduction
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