Capillary electrophoresis of high-molecular chitosan: The natural carbohydrate biopolymer

Due to its high resolving power and diverse application range, capillary electrophoresis (CE) has been successfully applied to the analysis of carbohydrates. In this paper, a method for the determination of high‐molecular chitosan (Mr 200 000) using CE is presented. We studied the optimal condition...

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Veröffentlicht in:Electrophoresis 2001-07, Vol.22 (11), p.2217-2221
Hauptverfasser: Ban, Eunmi, Choi, One-Kyun, Ryu, Jae-Chun, Yoo, Young S.
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container_title Electrophoresis
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creator Ban, Eunmi
Choi, One-Kyun
Ryu, Jae-Chun
Yoo, Young S.
description Due to its high resolving power and diverse application range, capillary electrophoresis (CE) has been successfully applied to the analysis of carbohydrates. In this paper, a method for the determination of high‐molecular chitosan (Mr 200 000) using CE is presented. We studied the optimal condition of buffer pH and type, and column type for determination of chitosan. Optimal CE performance was found when employing 100 mM triethylamine (TEA)‐phosphate buffer, pH 2.0 and untreated fused‐silica capillary (50 νm×27 cm) for the chitosan analysis. Under optimum conditions, excellent linear responses were obtained in the concentration range of 1.25–20 νM, with a linear correlation coefficient of 0.9983. The standard deviations of the migration time and peak area were found to be 2.5 and 6.4%, respectively. This method could be readily applied to chitosan determination in real biological samples and commercial products.
doi_str_mv 10.1002/1522-2683(20017)22:11<2217::AID-ELPS2217>3.0.CO;2-R
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source MEDLINE; Wiley Online Library Journals Frontfile Complete
subjects Animals
Biopolymers - chemistry
Biopolymers - isolation & purification
Buffers
Capillary electrophoresis
Carbohydrate Sequence
Carbohydrates
Chitin - analogs & derivatives
Chitin - blood
Chitin - chemistry
Chitin - isolation & purification
Chitosan
Electrophoresis, Capillary - methods
Ethylamines
Food Analysis - methods
Hydrogen-Ion Concentration
Molecular Sequence Data
Molecular Structure
Molecular Weight
Polysaccharides
Rats
title Capillary electrophoresis of high-molecular chitosan: The natural carbohydrate biopolymer
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