Capillary electrophoresis of high-molecular chitosan: The natural carbohydrate biopolymer
Due to its high resolving power and diverse application range, capillary electrophoresis (CE) has been successfully applied to the analysis of carbohydrates. In this paper, a method for the determination of high‐molecular chitosan (Mr 200 000) using CE is presented. We studied the optimal condition...
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Veröffentlicht in: | Electrophoresis 2001-07, Vol.22 (11), p.2217-2221 |
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description | Due to its high resolving power and diverse application range, capillary electrophoresis (CE) has been successfully applied to the analysis of carbohydrates. In this paper, a method for the determination of high‐molecular chitosan (Mr 200 000) using CE is presented. We studied the optimal condition of buffer pH and type, and column type for determination of chitosan. Optimal CE performance was found when employing 100 mM triethylamine (TEA)‐phosphate buffer, pH 2.0 and untreated fused‐silica capillary (50 νm×27 cm) for the chitosan analysis. Under optimum conditions, excellent linear responses were obtained in the concentration range of 1.25–20 νM, with a linear correlation coefficient of 0.9983. The standard deviations of the migration time and peak area were found to be 2.5 and 6.4%, respectively. This method could be readily applied to chitosan determination in real biological samples and commercial products. |
doi_str_mv | 10.1002/1522-2683(20017)22:11<2217::AID-ELPS2217>3.0.CO;2-R |
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In this paper, a method for the determination of high‐molecular chitosan (Mr 200 000) using CE is presented. We studied the optimal condition of buffer pH and type, and column type for determination of chitosan. Optimal CE performance was found when employing 100 mM triethylamine (TEA)‐phosphate buffer, pH 2.0 and untreated fused‐silica capillary (50 νm×27 cm) for the chitosan analysis. Under optimum conditions, excellent linear responses were obtained in the concentration range of 1.25–20 νM, with a linear correlation coefficient of 0.9983. The standard deviations of the migration time and peak area were found to be 2.5 and 6.4%, respectively. This method could be readily applied to chitosan determination in real biological samples and commercial products.</description><identifier>ISSN: 0173-0835</identifier><identifier>EISSN: 1522-2683</identifier><identifier>DOI: 10.1002/1522-2683(20017)22:11<2217::AID-ELPS2217>3.0.CO;2-R</identifier><identifier>PMID: 11504055</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biopolymers - chemistry ; Biopolymers - isolation & purification ; Buffers ; Capillary electrophoresis ; Carbohydrate Sequence ; Carbohydrates ; Chitin - analogs & derivatives ; Chitin - blood ; Chitin - chemistry ; Chitin - isolation & purification ; Chitosan ; Electrophoresis, Capillary - methods ; Ethylamines ; Food Analysis - methods ; Hydrogen-Ion Concentration ; Molecular Sequence Data ; Molecular Structure ; Molecular Weight ; Polysaccharides ; Rats</subject><ispartof>Electrophoresis, 2001-07, Vol.22 (11), p.2217-2221</ispartof><rights>Copyright © 2001 WILEY‐VCH Verlag GmbH, Weinheim, Fed. 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In this paper, a method for the determination of high‐molecular chitosan (Mr 200 000) using CE is presented. We studied the optimal condition of buffer pH and type, and column type for determination of chitosan. Optimal CE performance was found when employing 100 mM triethylamine (TEA)‐phosphate buffer, pH 2.0 and untreated fused‐silica capillary (50 νm×27 cm) for the chitosan analysis. Under optimum conditions, excellent linear responses were obtained in the concentration range of 1.25–20 νM, with a linear correlation coefficient of 0.9983. The standard deviations of the migration time and peak area were found to be 2.5 and 6.4%, respectively. This method could be readily applied to chitosan determination in real biological samples and commercial products.</description><subject>Animals</subject><subject>Biopolymers - chemistry</subject><subject>Biopolymers - isolation & purification</subject><subject>Buffers</subject><subject>Capillary electrophoresis</subject><subject>Carbohydrate Sequence</subject><subject>Carbohydrates</subject><subject>Chitin - analogs & derivatives</subject><subject>Chitin - blood</subject><subject>Chitin - chemistry</subject><subject>Chitin - isolation & purification</subject><subject>Chitosan</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Ethylamines</subject><subject>Food Analysis - methods</subject><subject>Hydrogen-Ion Concentration</subject><subject>Molecular Sequence Data</subject><subject>Molecular Structure</subject><subject>Molecular Weight</subject><subject>Polysaccharides</subject><subject>Rats</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkEGP0zAQhS0EYsvCX0A5ITikjO24TroIaRV2l0UVRdtFLKeRk9gkkNTBTgT99zi0lBMHTtZ43ryn9xFyRmFOAdhLKhiL2SLlzxkAlS8YW1L6ijEql8vz6zfxxerDZppe8znM8_UZi2_ukdnx6j6ZhSseQ8rFCXnk_VcASLIkeUhOKBWQgBAz8jlXfdO2yu0i3epycLavrdO-8ZE1Ud18qePOhsUYJFFZN4P1aruMbmsdbdUwOtVGpXKFrXeVU4OOisb2tt112j0mD4xqvX5yeE_Jx8uL2_xtvFpfXefnq7jkKZdxVsiikqU0KjOiSjjnGVVSQ8FomSSZqECY0NJwY2jGwYBIyzRLIcsoZzQUPCXP9r69s99H7QfsGl_q0Gmr7ehRUkgzuaBBuNkLS2e9d9pg75ouNEcKOBHHiR1O7PA3cQwTpTgxRgzE8Q9x5AiYr5HhTXB9eogfi05Xfz0PiIPgbi_40bR69z-Z_4g8_gXreG_d-EH_PFor9w0XkkuBn95f4Z0Ally-2yDwXzaYqb0</recordid><startdate>200107</startdate><enddate>200107</enddate><creator>Ban, Eunmi</creator><creator>Choi, One-Kyun</creator><creator>Ryu, Jae-Chun</creator><creator>Yoo, Young S.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200107</creationdate><title>Capillary electrophoresis of high-molecular chitosan: The natural carbohydrate biopolymer</title><author>Ban, Eunmi ; Choi, One-Kyun ; Ryu, Jae-Chun ; Yoo, Young S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3837-9b7bd7c7fa9f5d433391a7e0b21c4495d05f221f3ff1930f058c8980991321173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Biopolymers - chemistry</topic><topic>Biopolymers - isolation & purification</topic><topic>Buffers</topic><topic>Capillary electrophoresis</topic><topic>Carbohydrate Sequence</topic><topic>Carbohydrates</topic><topic>Chitin - analogs & derivatives</topic><topic>Chitin - blood</topic><topic>Chitin - chemistry</topic><topic>Chitin - isolation & purification</topic><topic>Chitosan</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Ethylamines</topic><topic>Food Analysis - methods</topic><topic>Hydrogen-Ion Concentration</topic><topic>Molecular Sequence Data</topic><topic>Molecular Structure</topic><topic>Molecular Weight</topic><topic>Polysaccharides</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ban, Eunmi</creatorcontrib><creatorcontrib>Choi, One-Kyun</creatorcontrib><creatorcontrib>Ryu, Jae-Chun</creatorcontrib><creatorcontrib>Yoo, Young S.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ban, Eunmi</au><au>Choi, One-Kyun</au><au>Ryu, Jae-Chun</au><au>Yoo, Young S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Capillary electrophoresis of high-molecular chitosan: The natural carbohydrate biopolymer</atitle><jtitle>Electrophoresis</jtitle><addtitle>ELECTROPHORESIS</addtitle><date>2001-07</date><risdate>2001</risdate><volume>22</volume><issue>11</issue><spage>2217</spage><epage>2221</epage><pages>2217-2221</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>Due to its high resolving power and diverse application range, capillary electrophoresis (CE) has been successfully applied to the analysis of carbohydrates. 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subjects | Animals Biopolymers - chemistry Biopolymers - isolation & purification Buffers Capillary electrophoresis Carbohydrate Sequence Carbohydrates Chitin - analogs & derivatives Chitin - blood Chitin - chemistry Chitin - isolation & purification Chitosan Electrophoresis, Capillary - methods Ethylamines Food Analysis - methods Hydrogen-Ion Concentration Molecular Sequence Data Molecular Structure Molecular Weight Polysaccharides Rats |
title | Capillary electrophoresis of high-molecular chitosan: The natural carbohydrate biopolymer |
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