Iba1 Is an Actin-Cross-Linking Protein in Macrophages/Microglia
Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/microglia and is upregulated during the activation of these cells. When exposed to macrophage colony-stimulating factor (M-CSF), microglia cell line MG5 immediately produces intense membrane ruffles in which Iba1 accumula...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 2001-08, Vol.286 (2), p.292-297 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 297 |
|---|---|
| container_issue | 2 |
| container_start_page | 292 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 286 |
| creator | Sasaki, Yo Ohsawa, Keiko Kanazawa, Hiroko Kohsaka, Shinichi Imai, Yoshinori |
| description | Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/microglia and is upregulated during the activation of these cells. When exposed to macrophage colony-stimulating factor (M-CSF), microglia cell line MG5 immediately produces intense membrane ruffles in which Iba1 accumulates together with filamentous actin. In this study, we investigated the physical interaction between Iba1 and actin by centrifugation assay and electron microscopic examination and showed that Iba1 possesses actin-binding and -cross-linking activities. Inhibitory mutant Iba1 that suppresses M-CSF-induced membrane ruffling had lost the actin-cross-linking activity, and it inhibited the cross-linking activity of intact Iba1. These results indicate that Iba1 is a macrophage/microglia-specific actin-cross-linking protein essential for M-CSF-induced membrane ruffling. |
| doi_str_mv | 10.1006/bbrc.2001.5388 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71085360</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X01953884</els_id><sourcerecordid>17926904</sourcerecordid><originalsourceid>FETCH-LOGICAL-c437t-16fa1c94459fbecac058ec1f21b8b2b7c6c6133309614ad7967e33e49dcfb8ef3</originalsourceid><addsrcrecordid>eNqFkE1Lw0AQhhdRbK1ePUpO3pLuZJNN9iSl-FFo0YOCt2V3M6mraVJ3U8F_74YWPIkwMAPzzAvzEHIJNAFK-VRrZ5KUUkhyVpZHZAxU0DgFmh2TMQ1EnAp4HZEz798DBRkXp2QEkIcdy8fkZqEVRAsfqTaamd628dx13sdL237Ydh09ua5H20ahVsq4bvum1uinKxvmdWPVOTmpVePx4tAn5OXu9nn-EC8f7xfz2TI2GSv6GHitwIgsy0Wt0ShD8xIN1CnoUqe6MNxwYIxRwSFTVSF4gYxhJipT6xJrNiHX-9yt6z536Hu5sd5g06gWu52XBdAyZ5z-C0IhUi5oFsBkD5rhYYe13Dq7Ue5bApWDWzm4lYNbObgNB1eH5J3eYPWLH2QGoNwDGER8WXTSG4utwco6NL2sOvtX9g-mF4bJ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17926904</pqid></control><display><type>article</type><title>Iba1 Is an Actin-Cross-Linking Protein in Macrophages/Microglia</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Sasaki, Yo ; Ohsawa, Keiko ; Kanazawa, Hiroko ; Kohsaka, Shinichi ; Imai, Yoshinori</creator><creatorcontrib>Sasaki, Yo ; Ohsawa, Keiko ; Kanazawa, Hiroko ; Kohsaka, Shinichi ; Imai, Yoshinori</creatorcontrib><description>Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/microglia and is upregulated during the activation of these cells. When exposed to macrophage colony-stimulating factor (M-CSF), microglia cell line MG5 immediately produces intense membrane ruffles in which Iba1 accumulates together with filamentous actin. In this study, we investigated the physical interaction between Iba1 and actin by centrifugation assay and electron microscopic examination and showed that Iba1 possesses actin-binding and -cross-linking activities. Inhibitory mutant Iba1 that suppresses M-CSF-induced membrane ruffling had lost the actin-cross-linking activity, and it inhibited the cross-linking activity of intact Iba1. These results indicate that Iba1 is a macrophage/microglia-specific actin-cross-linking protein essential for M-CSF-induced membrane ruffling.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.2001.5388</identifier><identifier>PMID: 11500035</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>actin ; Actins - metabolism ; Actins - ultrastructure ; Animals ; Calcium-Binding Proteins - genetics ; Calcium-Binding Proteins - physiology ; Cytoskeleton - metabolism ; Cytoskeleton - ultrastructure ; Iba1 ; Iba1 protein ; Macrophages - metabolism ; membrane-ruffling ; microglia ; Microglia - metabolism ; Mutation</subject><ispartof>Biochemical and biophysical research communications, 2001-08, Vol.286 (2), p.292-297</ispartof><rights>2001 Academic Press</rights><rights>Copyright 2001 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-16fa1c94459fbecac058ec1f21b8b2b7c6c6133309614ad7967e33e49dcfb8ef3</citedby><cites>FETCH-LOGICAL-c437t-16fa1c94459fbecac058ec1f21b8b2b7c6c6133309614ad7967e33e49dcfb8ef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X01953884$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11500035$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sasaki, Yo</creatorcontrib><creatorcontrib>Ohsawa, Keiko</creatorcontrib><creatorcontrib>Kanazawa, Hiroko</creatorcontrib><creatorcontrib>Kohsaka, Shinichi</creatorcontrib><creatorcontrib>Imai, Yoshinori</creatorcontrib><title>Iba1 Is an Actin-Cross-Linking Protein in Macrophages/Microglia</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/microglia and is upregulated during the activation of these cells. When exposed to macrophage colony-stimulating factor (M-CSF), microglia cell line MG5 immediately produces intense membrane ruffles in which Iba1 accumulates together with filamentous actin. In this study, we investigated the physical interaction between Iba1 and actin by centrifugation assay and electron microscopic examination and showed that Iba1 possesses actin-binding and -cross-linking activities. Inhibitory mutant Iba1 that suppresses M-CSF-induced membrane ruffling had lost the actin-cross-linking activity, and it inhibited the cross-linking activity of intact Iba1. These results indicate that Iba1 is a macrophage/microglia-specific actin-cross-linking protein essential for M-CSF-induced membrane ruffling.</description><subject>actin</subject><subject>Actins - metabolism</subject><subject>Actins - ultrastructure</subject><subject>Animals</subject><subject>Calcium-Binding Proteins - genetics</subject><subject>Calcium-Binding Proteins - physiology</subject><subject>Cytoskeleton - metabolism</subject><subject>Cytoskeleton - ultrastructure</subject><subject>Iba1</subject><subject>Iba1 protein</subject><subject>Macrophages - metabolism</subject><subject>membrane-ruffling</subject><subject>microglia</subject><subject>Microglia - metabolism</subject><subject>Mutation</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1Lw0AQhhdRbK1ePUpO3pLuZJNN9iSl-FFo0YOCt2V3M6mraVJ3U8F_74YWPIkwMAPzzAvzEHIJNAFK-VRrZ5KUUkhyVpZHZAxU0DgFmh2TMQ1EnAp4HZEz798DBRkXp2QEkIcdy8fkZqEVRAsfqTaamd628dx13sdL237Ydh09ua5H20ahVsq4bvum1uinKxvmdWPVOTmpVePx4tAn5OXu9nn-EC8f7xfz2TI2GSv6GHitwIgsy0Wt0ShD8xIN1CnoUqe6MNxwYIxRwSFTVSF4gYxhJipT6xJrNiHX-9yt6z536Hu5sd5g06gWu52XBdAyZ5z-C0IhUi5oFsBkD5rhYYe13Dq7Ue5bApWDWzm4lYNbObgNB1eH5J3eYPWLH2QGoNwDGER8WXTSG4utwco6NL2sOvtX9g-mF4bJ</recordid><startdate>20010817</startdate><enddate>20010817</enddate><creator>Sasaki, Yo</creator><creator>Ohsawa, Keiko</creator><creator>Kanazawa, Hiroko</creator><creator>Kohsaka, Shinichi</creator><creator>Imai, Yoshinori</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20010817</creationdate><title>Iba1 Is an Actin-Cross-Linking Protein in Macrophages/Microglia</title><author>Sasaki, Yo ; Ohsawa, Keiko ; Kanazawa, Hiroko ; Kohsaka, Shinichi ; Imai, Yoshinori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-16fa1c94459fbecac058ec1f21b8b2b7c6c6133309614ad7967e33e49dcfb8ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>actin</topic><topic>Actins - metabolism</topic><topic>Actins - ultrastructure</topic><topic>Animals</topic><topic>Calcium-Binding Proteins - genetics</topic><topic>Calcium-Binding Proteins - physiology</topic><topic>Cytoskeleton - metabolism</topic><topic>Cytoskeleton - ultrastructure</topic><topic>Iba1</topic><topic>Iba1 protein</topic><topic>Macrophages - metabolism</topic><topic>membrane-ruffling</topic><topic>microglia</topic><topic>Microglia - metabolism</topic><topic>Mutation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sasaki, Yo</creatorcontrib><creatorcontrib>Ohsawa, Keiko</creatorcontrib><creatorcontrib>Kanazawa, Hiroko</creatorcontrib><creatorcontrib>Kohsaka, Shinichi</creatorcontrib><creatorcontrib>Imai, Yoshinori</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sasaki, Yo</au><au>Ohsawa, Keiko</au><au>Kanazawa, Hiroko</au><au>Kohsaka, Shinichi</au><au>Imai, Yoshinori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Iba1 Is an Actin-Cross-Linking Protein in Macrophages/Microglia</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2001-08-17</date><risdate>2001</risdate><volume>286</volume><issue>2</issue><spage>292</spage><epage>297</epage><pages>292-297</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/microglia and is upregulated during the activation of these cells. When exposed to macrophage colony-stimulating factor (M-CSF), microglia cell line MG5 immediately produces intense membrane ruffles in which Iba1 accumulates together with filamentous actin. In this study, we investigated the physical interaction between Iba1 and actin by centrifugation assay and electron microscopic examination and showed that Iba1 possesses actin-binding and -cross-linking activities. Inhibitory mutant Iba1 that suppresses M-CSF-induced membrane ruffling had lost the actin-cross-linking activity, and it inhibited the cross-linking activity of intact Iba1. These results indicate that Iba1 is a macrophage/microglia-specific actin-cross-linking protein essential for M-CSF-induced membrane ruffling.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11500035</pmid><doi>10.1006/bbrc.2001.5388</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2001-08, Vol.286 (2), p.292-297 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71085360 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | actin Actins - metabolism Actins - ultrastructure Animals Calcium-Binding Proteins - genetics Calcium-Binding Proteins - physiology Cytoskeleton - metabolism Cytoskeleton - ultrastructure Iba1 Iba1 protein Macrophages - metabolism membrane-ruffling microglia Microglia - metabolism Mutation |
| title | Iba1 Is an Actin-Cross-Linking Protein in Macrophages/Microglia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T10%3A55%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Iba1%20Is%20an%20Actin-Cross-Linking%20Protein%20in%20Macrophages/Microglia&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Sasaki,%20Yo&rft.date=2001-08-17&rft.volume=286&rft.issue=2&rft.spage=292&rft.epage=297&rft.pages=292-297&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1006/bbrc.2001.5388&rft_dat=%3Cproquest_cross%3E17926904%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17926904&rft_id=info:pmid/11500035&rft_els_id=S0006291X01953884&rfr_iscdi=true |