A Sandwich ELISA for Detection of Soluble GPI-80, a Glycosylphosphatidyl-Inositol (GPI)-Anchored Protein on Human Leukocytes Involved in Regulation of Neutrophil Adherence and Migration-Its Release from Activated Neutrophils and Presence in Synovial Fluid of Rheumatoid Arthritis Patients
GPI‐80, mainly distributed on human neutrophils and monocytes, is a novel glycosylphosphatidylinositol (GPI)‐anchored protein regulating neutrophil adherence and migration. We prepared a polyclonal antibody specific to GPI‐80 by immunizing rabbits with the purified GPI‐80 and established a sandwich...
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Veröffentlicht in: | Microbiology and immunology 2001-01, Vol.45 (6), p.467-471 |
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description | GPI‐80, mainly distributed on human neutrophils and monocytes, is a novel glycosylphosphatidylinositol (GPI)‐anchored protein regulating neutrophil adherence and migration. We prepared a polyclonal antibody specific to GPI‐80 by immunizing rabbits with the purified GPI‐80 and established a sandwich ELISA for detecting soluble GPI‐80. We found that soluble GPI‐80 was released from fMLP activated neutrophils and was present at high concentrations in synovial fluids but not sera of rheumatoid arthritis patients, suggesting that GPI‐80 may play a role in inflammatory diseases. The detection of soluble GPI‐80 may help us to understand its physiological and pathological functions. |
doi_str_mv | 10.1111/j.1348-0421.2001.tb02646.x |
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We prepared a polyclonal antibody specific to GPI‐80 by immunizing rabbits with the purified GPI‐80 and established a sandwich ELISA for detecting soluble GPI‐80. We found that soluble GPI‐80 was released from fMLP activated neutrophils and was present at high concentrations in synovial fluids but not sera of rheumatoid arthritis patients, suggesting that GPI‐80 may play a role in inflammatory diseases. The detection of soluble GPI‐80 may help us to understand its physiological and pathological functions.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.2001.tb02646.x</identifier><identifier>PMID: 11497222</identifier><identifier>CODEN: MIIMDV</identifier><language>eng</language><publisher>Tokyo: Blackwell Publishing Ltd</publisher><subject>Amidohydrolases ; Animals ; Antibodies, Monoclonal ; Arthritis, Rheumatoid - blood ; Arthritis, Rheumatoid - metabolism ; Biological and medical sciences ; Cell Adhesion ; Cell Adhesion Molecules - analysis ; Cell Adhesion Molecules - biosynthesis ; Cell Adhesion Molecules - immunology ; Cell Movement ; Cells, Cultured ; Diverse techniques ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; GPI-Linked Proteins ; Humans ; Hydrolases ; Immune Sera ; Immunobiology ; Lymphocyte Activation ; Microbiology ; Molecular and cellular biology ; Myeloid cells: ontogeny, maturation, markers, receptors ; neutrophils ; Neutrophils - immunology ; Neutrophils - metabolism ; Polynuclears ; Rabbits ; sandwich ELISA ; soluble GPI-80 ; Synovial Fluid - metabolism ; synovial fluids</subject><ispartof>Microbiology and immunology, 2001-01, Vol.45 (6), p.467-471</ispartof><rights>owned by Center for Academic Publications Japan (Publisher)</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6386-68106ba615fe6a67d8dbad7867ccb97bd0dccd8409679e5a5dd017743fb940543</citedby><cites>FETCH-LOGICAL-c6386-68106ba615fe6a67d8dbad7867ccb97bd0dccd8409679e5a5dd017743fb940543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1348-0421.2001.tb02646.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1348-0421.2001.tb02646.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14165365$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11497222$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Jibiao</creatorcontrib><creatorcontrib>Takeda, Yuji</creatorcontrib><creatorcontrib>Watanabe, Tadashi</creatorcontrib><creatorcontrib>Sendo, Fujiro</creatorcontrib><title>A Sandwich ELISA for Detection of Soluble GPI-80, a Glycosylphosphatidyl-Inositol (GPI)-Anchored Protein on Human Leukocytes Involved in Regulation of Neutrophil Adherence and Migration-Its Release from Activated Neutrophils and Presence in Synovial Fluid of Rheumatoid Arthritis Patients</title><title>Microbiology and immunology</title><addtitle>Microbiology and Immunology</addtitle><description>GPI‐80, mainly distributed on human neutrophils and monocytes, is a novel glycosylphosphatidylinositol (GPI)‐anchored protein regulating neutrophil adherence and migration. We prepared a polyclonal antibody specific to GPI‐80 by immunizing rabbits with the purified GPI‐80 and established a sandwich ELISA for detecting soluble GPI‐80. We found that soluble GPI‐80 was released from fMLP activated neutrophils and was present at high concentrations in synovial fluids but not sera of rheumatoid arthritis patients, suggesting that GPI‐80 may play a role in inflammatory diseases. The detection of soluble GPI‐80 may help us to understand its physiological and pathological functions.</description><subject>Amidohydrolases</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Arthritis, Rheumatoid - blood</subject><subject>Arthritis, Rheumatoid - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion</subject><subject>Cell Adhesion Molecules - analysis</subject><subject>Cell Adhesion Molecules - biosynthesis</subject><subject>Cell Adhesion Molecules - immunology</subject><subject>Cell Movement</subject><subject>Cells, Cultured</subject><subject>Diverse techniques</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>GPI-Linked Proteins</subject><subject>Humans</subject><subject>Hydrolases</subject><subject>Immune Sera</subject><subject>Immunobiology</subject><subject>Lymphocyte Activation</subject><subject>Microbiology</subject><subject>Molecular and cellular biology</subject><subject>Myeloid cells: ontogeny, maturation, markers, receptors</subject><subject>neutrophils</subject><subject>Neutrophils - immunology</subject><subject>Neutrophils - metabolism</subject><subject>Polynuclears</subject><subject>Rabbits</subject><subject>sandwich ELISA</subject><subject>soluble GPI-80</subject><subject>Synovial Fluid - metabolism</subject><subject>synovial fluids</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkl1v0zAYhQMCsTH4C8hCGgKJFDtxnIQrwti6QjfKCtql5djO4s2Ni-10y7_H_WC7RFiWbMvPOee9OFH0GsERCuvD9QiluIghTtAogRCNfA0Tgsno7nG0f__1JNqHaZHFGYFwL3ru3DWESZ4U-Fm0hxAu8yRJ9h-BCsxZJ24Vb8HxdDKvQGMs-CK95F6ZDpgGzI3uay3BeDaJC_geMDDWAzdu0MvWuGXLvBKDjiedccobDd4G8F1cdbw1Vgows8ZLFZw6cNovWAemsr8xfPDSgUm3MnoVoPB_Ia96zf6GnsveW7NslQaVaKWVHZcgDArO1JXdUPHEuyDSkjkJGmsWoAojr5gPdg9qtxHNrHQbh5AzHzqzUkyDE90rsc66aGUYzJvwqqxvrfLKgVkIkZ13L6KnDdNOvtydB9Gvk-OfR6fx9Pt4clRNY07SgsSkQJDUjKCskYSRXBSiZiIvSM55Xea1gIJzUWBYkryUGcuEgCjPcdrUJYYZTg-iN1vfpTW_e-k8XSjHpdask6Z3NEewwFkB_wmigkBcliiAH7cgt8Y5Kxu6tGrB7EARpOsi0Wu6bgtdt4Wui0R3RaJ3Qfxql9LXCykepLvmBOBwBzDHmW4s67hyDxxGJEvDPog-bblbpeXwHyPQs8nZ5hos4q2Fcl7e3Vswe0NJnuYZvTwf0zT_cfn5K8b0W_oHfc75Aw</recordid><startdate>20010101</startdate><enddate>20010101</enddate><creator>Huang, Jibiao</creator><creator>Takeda, Yuji</creator><creator>Watanabe, Tadashi</creator><creator>Sendo, Fujiro</creator><general>Blackwell Publishing Ltd</general><general>Center for Academic Publications Japan</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20010101</creationdate><title>A Sandwich ELISA for Detection of Soluble GPI-80, a Glycosylphosphatidyl-Inositol (GPI)-Anchored Protein on Human Leukocytes Involved in Regulation of Neutrophil Adherence and Migration-Its Release from Activated Neutrophils and Presence in Synovial Fluid of Rheumatoid Arthritis Patients</title><author>Huang, Jibiao ; Takeda, Yuji ; Watanabe, Tadashi ; Sendo, Fujiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6386-68106ba615fe6a67d8dbad7867ccb97bd0dccd8409679e5a5dd017743fb940543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amidohydrolases</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Arthritis, Rheumatoid - blood</topic><topic>Arthritis, Rheumatoid - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion</topic><topic>Cell Adhesion Molecules - analysis</topic><topic>Cell Adhesion Molecules - biosynthesis</topic><topic>Cell Adhesion Molecules - immunology</topic><topic>Cell Movement</topic><topic>Cells, Cultured</topic><topic>Diverse techniques</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>GPI-Linked Proteins</topic><topic>Humans</topic><topic>Hydrolases</topic><topic>Immune Sera</topic><topic>Immunobiology</topic><topic>Lymphocyte Activation</topic><topic>Microbiology</topic><topic>Molecular and cellular biology</topic><topic>Myeloid cells: ontogeny, maturation, markers, receptors</topic><topic>neutrophils</topic><topic>Neutrophils - immunology</topic><topic>Neutrophils - metabolism</topic><topic>Polynuclears</topic><topic>Rabbits</topic><topic>sandwich ELISA</topic><topic>soluble GPI-80</topic><topic>Synovial Fluid - metabolism</topic><topic>synovial fluids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Jibiao</creatorcontrib><creatorcontrib>Takeda, Yuji</creatorcontrib><creatorcontrib>Watanabe, Tadashi</creatorcontrib><creatorcontrib>Sendo, Fujiro</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Jibiao</au><au>Takeda, Yuji</au><au>Watanabe, Tadashi</au><au>Sendo, Fujiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Sandwich ELISA for Detection of Soluble GPI-80, a Glycosylphosphatidyl-Inositol (GPI)-Anchored Protein on Human Leukocytes Involved in Regulation of Neutrophil Adherence and Migration-Its Release from Activated Neutrophils and Presence in Synovial Fluid of Rheumatoid Arthritis Patients</atitle><jtitle>Microbiology and immunology</jtitle><addtitle>Microbiology and Immunology</addtitle><date>2001-01-01</date><risdate>2001</risdate><volume>45</volume><issue>6</issue><spage>467</spage><epage>471</epage><pages>467-471</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><coden>MIIMDV</coden><abstract>GPI‐80, mainly distributed on human neutrophils and monocytes, is a novel glycosylphosphatidylinositol (GPI)‐anchored protein regulating neutrophil adherence and migration. We prepared a polyclonal antibody specific to GPI‐80 by immunizing rabbits with the purified GPI‐80 and established a sandwich ELISA for detecting soluble GPI‐80. We found that soluble GPI‐80 was released from fMLP activated neutrophils and was present at high concentrations in synovial fluids but not sera of rheumatoid arthritis patients, suggesting that GPI‐80 may play a role in inflammatory diseases. The detection of soluble GPI‐80 may help us to understand its physiological and pathological functions.</abstract><cop>Tokyo</cop><pub>Blackwell Publishing Ltd</pub><pmid>11497222</pmid><doi>10.1111/j.1348-0421.2001.tb02646.x</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Amidohydrolases Animals Antibodies, Monoclonal Arthritis, Rheumatoid - blood Arthritis, Rheumatoid - metabolism Biological and medical sciences Cell Adhesion Cell Adhesion Molecules - analysis Cell Adhesion Molecules - biosynthesis Cell Adhesion Molecules - immunology Cell Movement Cells, Cultured Diverse techniques Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Fundamental immunology GPI-Linked Proteins Humans Hydrolases Immune Sera Immunobiology Lymphocyte Activation Microbiology Molecular and cellular biology Myeloid cells: ontogeny, maturation, markers, receptors neutrophils Neutrophils - immunology Neutrophils - metabolism Polynuclears Rabbits sandwich ELISA soluble GPI-80 Synovial Fluid - metabolism synovial fluids |
title | A Sandwich ELISA for Detection of Soluble GPI-80, a Glycosylphosphatidyl-Inositol (GPI)-Anchored Protein on Human Leukocytes Involved in Regulation of Neutrophil Adherence and Migration-Its Release from Activated Neutrophils and Presence in Synovial Fluid of Rheumatoid Arthritis Patients |
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