A Sandwich ELISA for Detection of Soluble GPI-80, a Glycosylphosphatidyl-Inositol (GPI)-Anchored Protein on Human Leukocytes Involved in Regulation of Neutrophil Adherence and Migration-Its Release from Activated Neutrophils and Presence in Synovial Fluid of Rheumatoid Arthritis Patients

GPI‐80, mainly distributed on human neutrophils and monocytes, is a novel glycosylphosphatidylinositol (GPI)‐anchored protein regulating neutrophil adherence and migration. We prepared a polyclonal antibody specific to GPI‐80 by immunizing rabbits with the purified GPI‐80 and established a sandwich...

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Veröffentlicht in:Microbiology and immunology 2001-01, Vol.45 (6), p.467-471
Hauptverfasser: Huang, Jibiao, Takeda, Yuji, Watanabe, Tadashi, Sendo, Fujiro
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container_title Microbiology and immunology
container_volume 45
creator Huang, Jibiao
Takeda, Yuji
Watanabe, Tadashi
Sendo, Fujiro
description GPI‐80, mainly distributed on human neutrophils and monocytes, is a novel glycosylphosphatidylinositol (GPI)‐anchored protein regulating neutrophil adherence and migration. We prepared a polyclonal antibody specific to GPI‐80 by immunizing rabbits with the purified GPI‐80 and established a sandwich ELISA for detecting soluble GPI‐80. We found that soluble GPI‐80 was released from fMLP activated neutrophils and was present at high concentrations in synovial fluids but not sera of rheumatoid arthritis patients, suggesting that GPI‐80 may play a role in inflammatory diseases. The detection of soluble GPI‐80 may help us to understand its physiological and pathological functions.
doi_str_mv 10.1111/j.1348-0421.2001.tb02646.x
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Psychology</subject><subject>Fundamental immunology</subject><subject>GPI-Linked Proteins</subject><subject>Humans</subject><subject>Hydrolases</subject><subject>Immune Sera</subject><subject>Immunobiology</subject><subject>Lymphocyte Activation</subject><subject>Microbiology</subject><subject>Molecular and cellular biology</subject><subject>Myeloid cells: ontogeny, maturation, markers, receptors</subject><subject>neutrophils</subject><subject>Neutrophils - immunology</subject><subject>Neutrophils - metabolism</subject><subject>Polynuclears</subject><subject>Rabbits</subject><subject>sandwich ELISA</subject><subject>soluble GPI-80</subject><subject>Synovial Fluid - metabolism</subject><subject>synovial fluids</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkl1v0zAYhQMCsTH4C8hCGgKJFDtxnIQrwti6QjfKCtql5djO4s2Ni-10y7_H_WC7RFiWbMvPOee9OFH0GsERCuvD9QiluIghTtAogRCNfA0Tgsno7nG0f__1JNqHaZHFGYFwL3ru3DWESZ4U-Fm0hxAu8yRJ9h-BCsxZJ24Vb8HxdDKvQGMs-CK95F6ZDpgGzI3uay3BeDaJC_geMDDWAzdu0MvWuGXLvBKDjiedccobDd4G8F1cdbw1Vgows8ZLFZw6cNovWAemsr8xfPDSgUm3MnoVoPB_Ia96zf6GnsveW7NslQaVaKWVHZcgDArO1JXdUPHEuyDSkjkJGmsWoAojr5gPdg9qtxHNrHQbh5AzHzqzUkyDE90rsc66aGUYzJvwqqxvrfLKgVkIkZ13L6KnDdNOvtydB9Gvk-OfR6fx9Pt4clRNY07SgsSkQJDUjKCskYSRXBSiZiIvSM55Xea1gIJzUWBYkryUGcuEgCjPcdrUJYYZTg-iN1vfpTW_e-k8XSjHpdask6Z3NEewwFkB_wmigkBcliiAH7cgt8Y5Kxu6tGrB7EARpOsi0Wu6bgtdt4Wui0R3RaJ3Qfxql9LXCykepLvmBOBwBzDHmW4s67hyDxxGJEvDPog-bblbpeXwHyPQs8nZ5hos4q2Fcl7e3Vswe0NJnuYZvTwf0zT_cfn5K8b0W_oHfc75Aw</recordid><startdate>20010101</startdate><enddate>20010101</enddate><creator>Huang, Jibiao</creator><creator>Takeda, Yuji</creator><creator>Watanabe, Tadashi</creator><creator>Sendo, Fujiro</creator><general>Blackwell Publishing Ltd</general><general>Center for Academic Publications Japan</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20010101</creationdate><title>A Sandwich ELISA for Detection of Soluble GPI-80, a Glycosylphosphatidyl-Inositol (GPI)-Anchored Protein on Human Leukocytes Involved in Regulation of Neutrophil Adherence and Migration-Its Release from Activated Neutrophils and Presence in Synovial Fluid of Rheumatoid Arthritis Patients</title><author>Huang, Jibiao ; Takeda, Yuji ; Watanabe, Tadashi ; Sendo, Fujiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6386-68106ba615fe6a67d8dbad7867ccb97bd0dccd8409679e5a5dd017743fb940543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amidohydrolases</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Arthritis, Rheumatoid - blood</topic><topic>Arthritis, Rheumatoid - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion</topic><topic>Cell Adhesion Molecules - analysis</topic><topic>Cell Adhesion Molecules - biosynthesis</topic><topic>Cell Adhesion Molecules - immunology</topic><topic>Cell Movement</topic><topic>Cells, Cultured</topic><topic>Diverse techniques</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>GPI-Linked Proteins</topic><topic>Humans</topic><topic>Hydrolases</topic><topic>Immune Sera</topic><topic>Immunobiology</topic><topic>Lymphocyte Activation</topic><topic>Microbiology</topic><topic>Molecular and cellular biology</topic><topic>Myeloid cells: ontogeny, maturation, markers, receptors</topic><topic>neutrophils</topic><topic>Neutrophils - immunology</topic><topic>Neutrophils - metabolism</topic><topic>Polynuclears</topic><topic>Rabbits</topic><topic>sandwich ELISA</topic><topic>soluble GPI-80</topic><topic>Synovial Fluid - metabolism</topic><topic>synovial fluids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Jibiao</creatorcontrib><creatorcontrib>Takeda, Yuji</creatorcontrib><creatorcontrib>Watanabe, Tadashi</creatorcontrib><creatorcontrib>Sendo, Fujiro</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Jibiao</au><au>Takeda, Yuji</au><au>Watanabe, Tadashi</au><au>Sendo, Fujiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Sandwich ELISA for Detection of Soluble GPI-80, a Glycosylphosphatidyl-Inositol (GPI)-Anchored Protein on Human Leukocytes Involved in Regulation of Neutrophil Adherence and Migration-Its Release from Activated Neutrophils and Presence in Synovial Fluid of Rheumatoid Arthritis Patients</atitle><jtitle>Microbiology and immunology</jtitle><addtitle>Microbiology and Immunology</addtitle><date>2001-01-01</date><risdate>2001</risdate><volume>45</volume><issue>6</issue><spage>467</spage><epage>471</epage><pages>467-471</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><coden>MIIMDV</coden><abstract>GPI‐80, mainly distributed on human neutrophils and monocytes, is a novel glycosylphosphatidylinositol (GPI)‐anchored protein regulating neutrophil adherence and migration. We prepared a polyclonal antibody specific to GPI‐80 by immunizing rabbits with the purified GPI‐80 and established a sandwich ELISA for detecting soluble GPI‐80. We found that soluble GPI‐80 was released from fMLP activated neutrophils and was present at high concentrations in synovial fluids but not sera of rheumatoid arthritis patients, suggesting that GPI‐80 may play a role in inflammatory diseases. The detection of soluble GPI‐80 may help us to understand its physiological and pathological functions.</abstract><cop>Tokyo</cop><pub>Blackwell Publishing Ltd</pub><pmid>11497222</pmid><doi>10.1111/j.1348-0421.2001.tb02646.x</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
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subjects Amidohydrolases
Animals
Antibodies, Monoclonal
Arthritis, Rheumatoid - blood
Arthritis, Rheumatoid - metabolism
Biological and medical sciences
Cell Adhesion
Cell Adhesion Molecules - analysis
Cell Adhesion Molecules - biosynthesis
Cell Adhesion Molecules - immunology
Cell Movement
Cells, Cultured
Diverse techniques
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Fundamental immunology
GPI-Linked Proteins
Humans
Hydrolases
Immune Sera
Immunobiology
Lymphocyte Activation
Microbiology
Molecular and cellular biology
Myeloid cells: ontogeny, maturation, markers, receptors
neutrophils
Neutrophils - immunology
Neutrophils - metabolism
Polynuclears
Rabbits
sandwich ELISA
soluble GPI-80
Synovial Fluid - metabolism
synovial fluids
title A Sandwich ELISA for Detection of Soluble GPI-80, a Glycosylphosphatidyl-Inositol (GPI)-Anchored Protein on Human Leukocytes Involved in Regulation of Neutrophil Adherence and Migration-Its Release from Activated Neutrophils and Presence in Synovial Fluid of Rheumatoid Arthritis Patients
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