Detection of Babesia caballi infection by enzyme-linked immunosorbent assay using recombinant 48-kDa merozoite rhoptry protein
The 48-kDa Babesia caballi merozoite rhoptry protein was expressed using a pGEX4T expression vector in Escherichia coli as glutathione S-transferase fusion protein (GST-BC48), and the expressed GST-BC48 was used in an ELISA to detect specific antibodies in serum samples. No cross-reaction was observ...
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| Veröffentlicht in: | International journal for parasitology 2000-04, Vol.30 (5), p.633-635 |
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| container_title | International journal for parasitology |
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| creator | Ikadai, Hiromi Osorio, Claudia Rocio Xuan, Xuenan Igarashi, Ikuo Kanemaru, Takumi Nagasawa, Hideyuki Fujisaki, Kozo Suzuki, Naoyoshi Mikami, Takeshi |
| description | The 48-kDa
Babesia caballi merozoite rhoptry protein was expressed using a pGEX4T expression vector in
Escherichia coli as glutathione S-transferase fusion protein (GST-BC48), and the expressed GST-BC48 was used in an ELISA to detect specific antibodies in serum samples. No cross-reaction was observed with sera from horses experimentally infected with
Babesia equi. GST-BC48 ELISA was a highly sensitive and specific test when compared with the CFT. A total of 209 horse sera obtained from Central Mongolia were examined with the GST-BC48 ELISA and 46.4% (97/209) were found to be seropositive for
B. caballi, suggesting that the GST-BC48 ELISA can be successfully used for both quarantine and epidemiological studies. |
| doi_str_mv | 10.1016/S0020-7519(00)00008-4 |
| format | Article |
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Babesia caballi merozoite rhoptry protein was expressed using a pGEX4T expression vector in
Escherichia coli as glutathione S-transferase fusion protein (GST-BC48), and the expressed GST-BC48 was used in an ELISA to detect specific antibodies in serum samples. No cross-reaction was observed with sera from horses experimentally infected with
Babesia equi. GST-BC48 ELISA was a highly sensitive and specific test when compared with the CFT. A total of 209 horse sera obtained from Central Mongolia were examined with the GST-BC48 ELISA and 46.4% (97/209) were found to be seropositive for
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Babesia caballi merozoite rhoptry protein was expressed using a pGEX4T expression vector in
Escherichia coli as glutathione S-transferase fusion protein (GST-BC48), and the expressed GST-BC48 was used in an ELISA to detect specific antibodies in serum samples. No cross-reaction was observed with sera from horses experimentally infected with
Babesia equi. GST-BC48 ELISA was a highly sensitive and specific test when compared with the CFT. A total of 209 horse sera obtained from Central Mongolia were examined with the GST-BC48 ELISA and 46.4% (97/209) were found to be seropositive for
B. caballi, suggesting that the GST-BC48 ELISA can be successfully used for both quarantine and epidemiological studies.</description><subject>Animal protozoal diseases</subject><subject>Animals</subject><subject>Antigens, Protozoan - immunology</subject><subject>Babesia - isolation & purification</subject><subject>Babesia caballi</subject><subject>Babesiosis - diagnosis</subject><subject>Biological and medical sciences</subject><subject>Cross Reactions</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>GST-BC48</subject><subject>Horse Diseases - parasitology</subject><subject>Horses</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Molecular Weight</subject><subject>Mongolia</subject><subject>Parasitic diseases</subject><subject>Protozoal diseases</subject><subject>Protozoan Proteins - immunology</subject><subject>Recombinant Proteins - immunology</subject><subject>Recombinant rhoptry protein</subject><subject>rhoptry protein</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQQC0EotvCTwD5gFA5BMaxs45PCFq-pEocgLM1cSZgmthbO0FKD_x23O4KuNWXkT1vZqx5jD0R8FKA2L76AlBDpRthTgFeQDltpe6xjWi1qUDI5j7b_EWO2HHOPwFEI5V6yI4EaG0arTfs9znN5GYfA48Df4sdZY_cYYfj6LkPwyHZrZzC9TpRNfpwST3307SEmGPqKMwcc8aVL9mH7zyRi1PnA5Z31VaX58gnSvE6-pl4-hF3c1r5LsWZfHjEHgw4Znp8iCfs2_t3X88-VhefP3w6e3NROdXquTI1atE11G6RSJl-6Lbgyn3oTU9OOUQlWqmEkVqBBASJrlYtGqqd6wXIE_Z837fMvVooz3by2dE4YqC4ZKsFtEI07Z2g0I0EqUwBmz3oUsw50WB3yU-YVivA3hiyt4bszfotgL01ZFWpe3oYsHQT9f9V7ZUU4NkBwOxwHBIG5_M_TpptrbYFe73HqKztl6dks_MUHPW-CJhtH_0dP_kDJa6vZA</recordid><startdate>20000424</startdate><enddate>20000424</enddate><creator>Ikadai, Hiromi</creator><creator>Osorio, Claudia Rocio</creator><creator>Xuan, Xuenan</creator><creator>Igarashi, Ikuo</creator><creator>Kanemaru, Takumi</creator><creator>Nagasawa, Hideyuki</creator><creator>Fujisaki, Kozo</creator><creator>Suzuki, Naoyoshi</creator><creator>Mikami, Takeshi</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20000424</creationdate><title>Detection of Babesia caballi infection by enzyme-linked immunosorbent assay using recombinant 48-kDa merozoite rhoptry protein</title><author>Ikadai, Hiromi ; 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Babesia caballi merozoite rhoptry protein was expressed using a pGEX4T expression vector in
Escherichia coli as glutathione S-transferase fusion protein (GST-BC48), and the expressed GST-BC48 was used in an ELISA to detect specific antibodies in serum samples. No cross-reaction was observed with sera from horses experimentally infected with
Babesia equi. GST-BC48 ELISA was a highly sensitive and specific test when compared with the CFT. A total of 209 horse sera obtained from Central Mongolia were examined with the GST-BC48 ELISA and 46.4% (97/209) were found to be seropositive for
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| subjects | Animal protozoal diseases Animals Antigens, Protozoan - immunology Babesia - isolation & purification Babesia caballi Babesiosis - diagnosis Biological and medical sciences Cross Reactions ELISA Enzyme-Linked Immunosorbent Assay - veterinary GST-BC48 Horse Diseases - parasitology Horses Infectious diseases Medical sciences Molecular Weight Mongolia Parasitic diseases Protozoal diseases Protozoan Proteins - immunology Recombinant Proteins - immunology Recombinant rhoptry protein rhoptry protein |
| title | Detection of Babesia caballi infection by enzyme-linked immunosorbent assay using recombinant 48-kDa merozoite rhoptry protein |
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