Footprinting methods for analysis of pyrrole-imidazole polyamide/DNA complexes

This chapter describes three complementary footprinting methods and the protocols used for analysis of polyamide : DNA complexes: (1) MPE–Fe(II) footprinting, (2) affinity cleavage, and (3) quantitative DNase I footprint titration. Footprinting with methidiumpropyl–EDTA–Fe(II) [MPE–Fe(II)] is used t...

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Veröffentlicht in:	Methods in Enzymology 2001, Vol.340, p.450-466
Hauptverfasser:	Trauger, John W, Dvan, Peter B
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Binding Sites Deoxyribonuclease I DNA - chemistry DNA Footprinting - methods DNA Footprinting - statistics & numerical data DNA Primers - genetics Drug Design Edetic Acid - analogs & derivatives Imidazoles - chemistry Ligands Macromolecular Substances Molecular Sequence Data Pyrroles - chemistry
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