Effect of selenium supplementation on the activities of glutathione metabolizing enzymes in human hepatoma Hep G2 cell line
Cell culture is an important tool for studying injury to cells exposed to oxidative stress. The human hepatoblastoma derived Hep G2 cells retain their morphology and most of their function in culture and are therefore widely used as an in vitro model of human hepatocytes. Conventional cell culture m...
Gespeichert in:
| Veröffentlicht in: | Toxicology (Amsterdam) 2000-04, Vol.144 (1), p.57-61 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 61 |
|---|---|
| container_issue | 1 |
| container_start_page | 57 |
| container_title | Toxicology (Amsterdam) |
| container_volume | 144 |
| creator | Helmy, Madiha H. Ismail, Saad S. Fayed, Hassan El-Bassiouni, Emad A. |
| description | Cell culture is an important tool for studying injury to cells exposed to oxidative stress. The human hepatoblastoma derived Hep G2 cells retain their morphology and most of their function in culture and are therefore widely used as an in vitro model of human hepatocytes. Conventional cell culture media are deficient in selenium, which is essential for activation of glutathione peroxidase (GPx), a key enzyme in the defense against oxidative stress. Supplementation of the culture media with 1 μM sodium selenite increased the activities of total GPx by threefold and the selenium-dependent GPx by fourfold as compared to cells cultured in control media. The non-selenium-dependent GPx activity was unchanged. The activities of the other glutathione (GSH)-related enzymes were practically unchanged despite a tendency toward elevation. The activities of oxidized glutathione (GSSG) reductase and catalase increased by 22.4 and 27.4%, respectively. These relatively small increases did not carry statistical significance. Supplementation of tissue culture media with selenium may prove important, particularly for cell protection against oxidative stress. |
| doi_str_mv | 10.1016/S0300-483X(99)00190-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71072094</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0300483X99001900</els_id><sourcerecordid>17527245</sourcerecordid><originalsourceid>FETCH-LOGICAL-c518t-efe5d7e7315563be4d3aec6e34b4a22704f33a79fb6b801c644a212cf57c36263</originalsourceid><addsrcrecordid>eNqF0VFr1TAUB_AgirtOP4KSBxF96DxJ2qZ5GjLmNhj4MAXfQpqe7EbatDbpYPPLm-5edG8XQgLJ7ySH_Al5y-CEAas_34AAKMpG_Pyo1CcApqCAZ2TDGqkKwZrqOdn8I0fkVYy_AICLsn5JjhjIJkO2IX_OnUOb6OhoxB6DXwYal2nqccCQTPJjoHmkLVJjk7_zyWNc9W2_5ONtPkc6YDLt2PsHH24phof7IRsf6HYZTJ5xMmkcDL3EiV5warHvae8DviYvnOkjvtmvx-TH1_PvZ5fF9beLq7Mv14WtWJMKdFh1EqVgVVWLFstOGLQ1irItDecSSieEkcq1ddsAs3WZtxm3rpJW1LwWx-TD7t5pHn8vGJMefFy7MAHHJWqZv4ODKg9CJisueVkdhtkoBZBhtYN2HmOc0elp9oOZ7zUDveaoH3PUa0haKf2Yo17r3u0fWNoBuydVu-AyeL8HJlrTu9kE6-N_JxTjTGR2umOY__fO46yj9Rgsdn7Osetu9Ac6-QvrOLsX</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14539900</pqid></control><display><type>article</type><title>Effect of selenium supplementation on the activities of glutathione metabolizing enzymes in human hepatoma Hep G2 cell line</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Helmy, Madiha H. ; Ismail, Saad S. ; Fayed, Hassan ; El-Bassiouni, Emad A.</creator><creatorcontrib>Helmy, Madiha H. ; Ismail, Saad S. ; Fayed, Hassan ; El-Bassiouni, Emad A.</creatorcontrib><description>Cell culture is an important tool for studying injury to cells exposed to oxidative stress. The human hepatoblastoma derived Hep G2 cells retain their morphology and most of their function in culture and are therefore widely used as an in vitro model of human hepatocytes. Conventional cell culture media are deficient in selenium, which is essential for activation of glutathione peroxidase (GPx), a key enzyme in the defense against oxidative stress. Supplementation of the culture media with 1 μM sodium selenite increased the activities of total GPx by threefold and the selenium-dependent GPx by fourfold as compared to cells cultured in control media. The non-selenium-dependent GPx activity was unchanged. The activities of the other glutathione (GSH)-related enzymes were practically unchanged despite a tendency toward elevation. The activities of oxidized glutathione (GSSG) reductase and catalase increased by 22.4 and 27.4%, respectively. These relatively small increases did not carry statistical significance. Supplementation of tissue culture media with selenium may prove important, particularly for cell protection against oxidative stress.</description><identifier>ISSN: 0300-483X</identifier><identifier>EISSN: 1879-3185</identifier><identifier>DOI: 10.1016/S0300-483X(99)00190-0</identifier><identifier>PMID: 10781871</identifier><identifier>CODEN: TXICDD</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Antioxidant defense ; Biological and medical sciences ; Catalase ; Catalase - metabolism ; Cell culture ; Cell Line ; Culture Media ; General and cellular metabolism. Vitamins ; Glutathione - metabolism ; Glutathione peroxidase ; Glutathione Peroxidase - metabolism ; Glutathione reductase ; Glutathione Reductase - metabolism ; Humans ; L-Lactate Dehydrogenase - metabolism ; Liver Neoplasms, Experimental - enzymology ; Medical sciences ; Pharmacology. Drug treatments ; Selenium ; Selenium - pharmacology ; Tumor Cells, Cultured</subject><ispartof>Toxicology (Amsterdam), 2000-04, Vol.144 (1), p.57-61</ispartof><rights>2000 Elsevier Science Ireland Ltd</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-efe5d7e7315563be4d3aec6e34b4a22704f33a79fb6b801c644a212cf57c36263</citedby><cites>FETCH-LOGICAL-c518t-efe5d7e7315563be4d3aec6e34b4a22704f33a79fb6b801c644a212cf57c36263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0300-483X(99)00190-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1391213$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10781871$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Helmy, Madiha H.</creatorcontrib><creatorcontrib>Ismail, Saad S.</creatorcontrib><creatorcontrib>Fayed, Hassan</creatorcontrib><creatorcontrib>El-Bassiouni, Emad A.</creatorcontrib><title>Effect of selenium supplementation on the activities of glutathione metabolizing enzymes in human hepatoma Hep G2 cell line</title><title>Toxicology (Amsterdam)</title><addtitle>Toxicology</addtitle><description>Cell culture is an important tool for studying injury to cells exposed to oxidative stress. The human hepatoblastoma derived Hep G2 cells retain their morphology and most of their function in culture and are therefore widely used as an in vitro model of human hepatocytes. Conventional cell culture media are deficient in selenium, which is essential for activation of glutathione peroxidase (GPx), a key enzyme in the defense against oxidative stress. Supplementation of the culture media with 1 μM sodium selenite increased the activities of total GPx by threefold and the selenium-dependent GPx by fourfold as compared to cells cultured in control media. The non-selenium-dependent GPx activity was unchanged. The activities of the other glutathione (GSH)-related enzymes were practically unchanged despite a tendency toward elevation. The activities of oxidized glutathione (GSSG) reductase and catalase increased by 22.4 and 27.4%, respectively. These relatively small increases did not carry statistical significance. Supplementation of tissue culture media with selenium may prove important, particularly for cell protection against oxidative stress.</description><subject>Animals</subject><subject>Antioxidant defense</subject><subject>Biological and medical sciences</subject><subject>Catalase</subject><subject>Catalase - metabolism</subject><subject>Cell culture</subject><subject>Cell Line</subject><subject>Culture Media</subject><subject>General and cellular metabolism. Vitamins</subject><subject>Glutathione - metabolism</subject><subject>Glutathione peroxidase</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Glutathione reductase</subject><subject>Glutathione Reductase - metabolism</subject><subject>Humans</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Liver Neoplasms, Experimental - enzymology</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Selenium</subject><subject>Selenium - pharmacology</subject><subject>Tumor Cells, Cultured</subject><issn>0300-483X</issn><issn>1879-3185</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0VFr1TAUB_AgirtOP4KSBxF96DxJ2qZ5GjLmNhj4MAXfQpqe7EbatDbpYPPLm-5edG8XQgLJ7ySH_Al5y-CEAas_34AAKMpG_Pyo1CcApqCAZ2TDGqkKwZrqOdn8I0fkVYy_AICLsn5JjhjIJkO2IX_OnUOb6OhoxB6DXwYal2nqccCQTPJjoHmkLVJjk7_zyWNc9W2_5ONtPkc6YDLt2PsHH24phof7IRsf6HYZTJ5xMmkcDL3EiV5warHvae8DviYvnOkjvtmvx-TH1_PvZ5fF9beLq7Mv14WtWJMKdFh1EqVgVVWLFstOGLQ1irItDecSSieEkcq1ddsAs3WZtxm3rpJW1LwWx-TD7t5pHn8vGJMefFy7MAHHJWqZv4ODKg9CJisueVkdhtkoBZBhtYN2HmOc0elp9oOZ7zUDveaoH3PUa0haKf2Yo17r3u0fWNoBuydVu-AyeL8HJlrTu9kE6-N_JxTjTGR2umOY__fO46yj9Rgsdn7Osetu9Ac6-QvrOLsX</recordid><startdate>20000403</startdate><enddate>20000403</enddate><creator>Helmy, Madiha H.</creator><creator>Ismail, Saad S.</creator><creator>Fayed, Hassan</creator><creator>El-Bassiouni, Emad A.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7U7</scope><scope>7X8</scope></search><sort><creationdate>20000403</creationdate><title>Effect of selenium supplementation on the activities of glutathione metabolizing enzymes in human hepatoma Hep G2 cell line</title><author>Helmy, Madiha H. ; Ismail, Saad S. ; Fayed, Hassan ; El-Bassiouni, Emad A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-efe5d7e7315563be4d3aec6e34b4a22704f33a79fb6b801c644a212cf57c36263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Antioxidant defense</topic><topic>Biological and medical sciences</topic><topic>Catalase</topic><topic>Catalase - metabolism</topic><topic>Cell culture</topic><topic>Cell Line</topic><topic>Culture Media</topic><topic>General and cellular metabolism. Vitamins</topic><topic>Glutathione - metabolism</topic><topic>Glutathione peroxidase</topic><topic>Glutathione Peroxidase - metabolism</topic><topic>Glutathione reductase</topic><topic>Glutathione Reductase - metabolism</topic><topic>Humans</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Liver Neoplasms, Experimental - enzymology</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Selenium</topic><topic>Selenium - pharmacology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Helmy, Madiha H.</creatorcontrib><creatorcontrib>Ismail, Saad S.</creatorcontrib><creatorcontrib>Fayed, Hassan</creatorcontrib><creatorcontrib>El-Bassiouni, Emad A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Toxicology Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Toxicology (Amsterdam)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Helmy, Madiha H.</au><au>Ismail, Saad S.</au><au>Fayed, Hassan</au><au>El-Bassiouni, Emad A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of selenium supplementation on the activities of glutathione metabolizing enzymes in human hepatoma Hep G2 cell line</atitle><jtitle>Toxicology (Amsterdam)</jtitle><addtitle>Toxicology</addtitle><date>2000-04-03</date><risdate>2000</risdate><volume>144</volume><issue>1</issue><spage>57</spage><epage>61</epage><pages>57-61</pages><issn>0300-483X</issn><eissn>1879-3185</eissn><coden>TXICDD</coden><abstract>Cell culture is an important tool for studying injury to cells exposed to oxidative stress. The human hepatoblastoma derived Hep G2 cells retain their morphology and most of their function in culture and are therefore widely used as an in vitro model of human hepatocytes. Conventional cell culture media are deficient in selenium, which is essential for activation of glutathione peroxidase (GPx), a key enzyme in the defense against oxidative stress. Supplementation of the culture media with 1 μM sodium selenite increased the activities of total GPx by threefold and the selenium-dependent GPx by fourfold as compared to cells cultured in control media. The non-selenium-dependent GPx activity was unchanged. The activities of the other glutathione (GSH)-related enzymes were practically unchanged despite a tendency toward elevation. The activities of oxidized glutathione (GSSG) reductase and catalase increased by 22.4 and 27.4%, respectively. These relatively small increases did not carry statistical significance. Supplementation of tissue culture media with selenium may prove important, particularly for cell protection against oxidative stress.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier Ireland Ltd</pub><pmid>10781871</pmid><doi>10.1016/S0300-483X(99)00190-0</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-483X |
| ispartof | Toxicology (Amsterdam), 2000-04, Vol.144 (1), p.57-61 |
| issn | 0300-483X 1879-3185 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71072094 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Antioxidant defense Biological and medical sciences Catalase Catalase - metabolism Cell culture Cell Line Culture Media General and cellular metabolism. Vitamins Glutathione - metabolism Glutathione peroxidase Glutathione Peroxidase - metabolism Glutathione reductase Glutathione Reductase - metabolism Humans L-Lactate Dehydrogenase - metabolism Liver Neoplasms, Experimental - enzymology Medical sciences Pharmacology. Drug treatments Selenium Selenium - pharmacology Tumor Cells, Cultured |
| title | Effect of selenium supplementation on the activities of glutathione metabolizing enzymes in human hepatoma Hep G2 cell line |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T09%3A03%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20selenium%20supplementation%20on%20the%20activities%20of%20glutathione%20metabolizing%20enzymes%20in%20human%20hepatoma%20Hep%20G2%20cell%20line&rft.jtitle=Toxicology%20(Amsterdam)&rft.au=Helmy,%20Madiha%20H.&rft.date=2000-04-03&rft.volume=144&rft.issue=1&rft.spage=57&rft.epage=61&rft.pages=57-61&rft.issn=0300-483X&rft.eissn=1879-3185&rft.coden=TXICDD&rft_id=info:doi/10.1016/S0300-483X(99)00190-0&rft_dat=%3Cproquest_cross%3E17527245%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14539900&rft_id=info:pmid/10781871&rft_els_id=S0300483X99001900&rfr_iscdi=true |