Calcium/calmodulin-dependent protein kinase II containing a nuclear localizing signal is altered in retinal neurons exposed to N-methyl- d-aspartate
This study investigated N-methyl- d-aspartate (NMDA) mediated cell death and its possible regulation by calcium/calmodulin-dependent protein kinase II (CaMKII) in the adult rat retina. To investigate cell death, the terminal deoxyribonucleotidyltransferase (TdT)-mediated biotin-16-dUTP nick-end labe...
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| Veröffentlicht in: | Brain research. Molecular brain research. 2000-03, Vol.76 (2), p.253-265 |
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| creator | Laabich, Aicha Li, Guangyu Cooper, Nigel G.F |
| description | This study investigated
N-methyl-
d-aspartate (NMDA) mediated cell death and its possible regulation by calcium/calmodulin-dependent protein kinase II (CaMKII) in the adult rat retina. To investigate cell death, the terminal deoxyribonucleotidyltransferase (TdT)-mediated biotin-16-dUTP nick-end labelling (TUNEL) method was used to detect fragmented DNA in fixed tissue sections of rat retina. The TUNEL assay confirmed that apoptosis occurs in the inner nuclear layer (INL) and ganglion cell layer (GCL) following NMDA injection. The level of antibody binding to CaMKII-α, the activity of CaMKII, and the mRNA level for the α
B subunit of CaMKII were found to be elevated for short time periods (30 min, 2 h) after a single intravitreal injection of NMDA. In contrast to this, there was a decrease in CaMKII activity and in the CaMKII-α
B mRNA levels at longer time periods (24 h) following injection of NMDA. These effects were specific for the mRNA for the α
B subunit, an alternatively spliced product of the CaMKII-α gene, that contains a nuclear localizing signal (NLS) known to target this protein to the nucleus. It is suggested that regulated expression of CaMKII-α
B could be involved in the NMDA-mediated cell death in retinal neurons. |
| doi_str_mv | 10.1016/S0169-328X(00)00006-1 |
| format | Article |
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N-methyl-
d-aspartate (NMDA) mediated cell death and its possible regulation by calcium/calmodulin-dependent protein kinase II (CaMKII) in the adult rat retina. To investigate cell death, the terminal deoxyribonucleotidyltransferase (TdT)-mediated biotin-16-dUTP nick-end labelling (TUNEL) method was used to detect fragmented DNA in fixed tissue sections of rat retina. The TUNEL assay confirmed that apoptosis occurs in the inner nuclear layer (INL) and ganglion cell layer (GCL) following NMDA injection. The level of antibody binding to CaMKII-α, the activity of CaMKII, and the mRNA level for the α
B subunit of CaMKII were found to be elevated for short time periods (30 min, 2 h) after a single intravitreal injection of NMDA. In contrast to this, there was a decrease in CaMKII activity and in the CaMKII-α
B mRNA levels at longer time periods (24 h) following injection of NMDA. These effects were specific for the mRNA for the α
B subunit, an alternatively spliced product of the CaMKII-α gene, that contains a nuclear localizing signal (NLS) known to target this protein to the nucleus. It is suggested that regulated expression of CaMKII-α
B could be involved in the NMDA-mediated cell death in retinal neurons.</description><identifier>ISSN: 0169-328X</identifier><identifier>EISSN: 1872-6941</identifier><identifier>DOI: 10.1016/S0169-328X(00)00006-1</identifier><identifier>PMID: 10762700</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Apoptosis ; Biological and medical sciences ; Calcium-Calmodulin-Dependent Protein Kinase Type 2 ; Calcium-Calmodulin-Dependent Protein Kinases - chemistry ; Calcium-Calmodulin-Dependent Protein Kinases - genetics ; Calcium-Calmodulin-Dependent Protein Kinases - metabolism ; CaMKII-α ; CaMKII-α B ; Cell Death ; Dizocilpine Maleate - pharmacology ; Eye and associated structures. Visual pathways and centers. Vision ; Fundamental and applied biological sciences. Psychology ; In Situ Nick-End Labeling ; Kinetics ; N-Methyl-D-aspartic acid ; N-Methylaspartate - pharmacology ; Neurons - drug effects ; Neurons - enzymology ; NMDA ; nuclear localization signal ; Nucleus ; Polymerase Chain Reaction ; Rat retina ; Rats ; Rats, Sprague-Dawley ; Retina - cytology ; Retina - enzymology ; Transcription, Genetic - drug effects ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research. Molecular brain research., 2000-03, Vol.76 (2), p.253-265</ispartof><rights>2000 Elsevier Science B.V.</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-74ac96cef7a24ba46792fb1783392ac7bcf42d51bb1921486bde7ac25b4deefc3</citedby><cites>FETCH-LOGICAL-c421t-74ac96cef7a24ba46792fb1783392ac7bcf42d51bb1921486bde7ac25b4deefc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1337577$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10762700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laabich, Aicha</creatorcontrib><creatorcontrib>Li, Guangyu</creatorcontrib><creatorcontrib>Cooper, Nigel G.F</creatorcontrib><title>Calcium/calmodulin-dependent protein kinase II containing a nuclear localizing signal is altered in retinal neurons exposed to N-methyl- d-aspartate</title><title>Brain research. Molecular brain research.</title><addtitle>Brain Res Mol Brain Res</addtitle><description>This study investigated
N-methyl-
d-aspartate (NMDA) mediated cell death and its possible regulation by calcium/calmodulin-dependent protein kinase II (CaMKII) in the adult rat retina. To investigate cell death, the terminal deoxyribonucleotidyltransferase (TdT)-mediated biotin-16-dUTP nick-end labelling (TUNEL) method was used to detect fragmented DNA in fixed tissue sections of rat retina. The TUNEL assay confirmed that apoptosis occurs in the inner nuclear layer (INL) and ganglion cell layer (GCL) following NMDA injection. The level of antibody binding to CaMKII-α, the activity of CaMKII, and the mRNA level for the α
B subunit of CaMKII were found to be elevated for short time periods (30 min, 2 h) after a single intravitreal injection of NMDA. In contrast to this, there was a decrease in CaMKII activity and in the CaMKII-α
B mRNA levels at longer time periods (24 h) following injection of NMDA. These effects were specific for the mRNA for the α
B subunit, an alternatively spliced product of the CaMKII-α gene, that contains a nuclear localizing signal (NLS) known to target this protein to the nucleus. It is suggested that regulated expression of CaMKII-α
B could be involved in the NMDA-mediated cell death in retinal neurons.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Calcium-Calmodulin-Dependent Protein Kinase Type 2</subject><subject>Calcium-Calmodulin-Dependent Protein Kinases - chemistry</subject><subject>Calcium-Calmodulin-Dependent Protein Kinases - genetics</subject><subject>Calcium-Calmodulin-Dependent Protein Kinases - metabolism</subject><subject>CaMKII-α</subject><subject>CaMKII-α B</subject><subject>Cell Death</subject><subject>Dizocilpine Maleate - pharmacology</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Situ Nick-End Labeling</subject><subject>Kinetics</subject><subject>N-Methyl-D-aspartic acid</subject><subject>N-Methylaspartate - pharmacology</subject><subject>Neurons - drug effects</subject><subject>Neurons - enzymology</subject><subject>NMDA</subject><subject>nuclear localization signal</subject><subject>Nucleus</subject><subject>Polymerase Chain Reaction</subject><subject>Rat retina</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Retina - cytology</subject><subject>Retina - enzymology</subject><subject>Transcription, Genetic - drug effects</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0169-328X</issn><issn>1872-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-O1SAUxhujce6MPoKGhTHjAgdaWtqVMTf-uclEF2rijpzC6YhSuAI1js_hA0vn3qi7YQHJ4fcdDt9XVY84e84Z7y4-lG2gTd1_PmfsGSuro_xOteG9rGk3CH632vxFTqrTlL4Whvec369OOJNdLRnbVL-34LRd5gsNbg5mcdZTg3v0Bn0m-xgyWk--WQ8JyW5HdPAZrLf-igDxi3YIkbhQ1PbXWkz2yoMjNhFwGSMaUuQRs12rHpcYfCL4cx9SucqBvKMz5i_XjhJDIe0hZsj4oLo3gUv48HieVZ9ev_q4fUsv37_ZbV9eUi1qnqkUoIdO4yShFiOITg71NHLZN81Qg5ajnkRtWj6OfKi56LvRoARdt6MwiJNuzqqnh77ln98XTFnNNml0DjyGJSlZfOJDJ28FuWwH0QhewPYA6hhSijipfbQzxGvFmVpzUze5qTUUxZi6yU2tusfHB5ZxRvOf6hBUAZ4cAUjF7CmC1zb945pGtnId9MUBw2LbD4tRJW3RazQ2os7KBHvLJH8AYM-39A</recordid><startdate>20000329</startdate><enddate>20000329</enddate><creator>Laabich, Aicha</creator><creator>Li, Guangyu</creator><creator>Cooper, Nigel G.F</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20000329</creationdate><title>Calcium/calmodulin-dependent protein kinase II containing a nuclear localizing signal is altered in retinal neurons exposed to N-methyl- d-aspartate</title><author>Laabich, Aicha ; Li, Guangyu ; Cooper, Nigel G.F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-74ac96cef7a24ba46792fb1783392ac7bcf42d51bb1921486bde7ac25b4deefc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Calcium-Calmodulin-Dependent Protein Kinase Type 2</topic><topic>Calcium-Calmodulin-Dependent Protein Kinases - chemistry</topic><topic>Calcium-Calmodulin-Dependent Protein Kinases - genetics</topic><topic>Calcium-Calmodulin-Dependent Protein Kinases - metabolism</topic><topic>CaMKII-α</topic><topic>CaMKII-α B</topic><topic>Cell Death</topic><topic>Dizocilpine Maleate - pharmacology</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Situ Nick-End Labeling</topic><topic>Kinetics</topic><topic>N-Methyl-D-aspartic acid</topic><topic>N-Methylaspartate - pharmacology</topic><topic>Neurons - drug effects</topic><topic>Neurons - enzymology</topic><topic>NMDA</topic><topic>nuclear localization signal</topic><topic>Nucleus</topic><topic>Polymerase Chain Reaction</topic><topic>Rat retina</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Retina - cytology</topic><topic>Retina - enzymology</topic><topic>Transcription, Genetic - drug effects</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laabich, Aicha</creatorcontrib><creatorcontrib>Li, Guangyu</creatorcontrib><creatorcontrib>Cooper, Nigel G.F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research. Molecular brain research.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laabich, Aicha</au><au>Li, Guangyu</au><au>Cooper, Nigel G.F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calcium/calmodulin-dependent protein kinase II containing a nuclear localizing signal is altered in retinal neurons exposed to N-methyl- d-aspartate</atitle><jtitle>Brain research. Molecular brain research.</jtitle><addtitle>Brain Res Mol Brain Res</addtitle><date>2000-03-29</date><risdate>2000</risdate><volume>76</volume><issue>2</issue><spage>253</spage><epage>265</epage><pages>253-265</pages><issn>0169-328X</issn><eissn>1872-6941</eissn><abstract>This study investigated
N-methyl-
d-aspartate (NMDA) mediated cell death and its possible regulation by calcium/calmodulin-dependent protein kinase II (CaMKII) in the adult rat retina. To investigate cell death, the terminal deoxyribonucleotidyltransferase (TdT)-mediated biotin-16-dUTP nick-end labelling (TUNEL) method was used to detect fragmented DNA in fixed tissue sections of rat retina. The TUNEL assay confirmed that apoptosis occurs in the inner nuclear layer (INL) and ganglion cell layer (GCL) following NMDA injection. The level of antibody binding to CaMKII-α, the activity of CaMKII, and the mRNA level for the α
B subunit of CaMKII were found to be elevated for short time periods (30 min, 2 h) after a single intravitreal injection of NMDA. In contrast to this, there was a decrease in CaMKII activity and in the CaMKII-α
B mRNA levels at longer time periods (24 h) following injection of NMDA. These effects were specific for the mRNA for the α
B subunit, an alternatively spliced product of the CaMKII-α gene, that contains a nuclear localizing signal (NLS) known to target this protein to the nucleus. It is suggested that regulated expression of CaMKII-α
B could be involved in the NMDA-mediated cell death in retinal neurons.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10762700</pmid><doi>10.1016/S0169-328X(00)00006-1</doi><tpages>13</tpages></addata></record> |
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| ispartof | Brain research. Molecular brain research., 2000-03, Vol.76 (2), p.253-265 |
| issn | 0169-328X 1872-6941 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Apoptosis Biological and medical sciences Calcium-Calmodulin-Dependent Protein Kinase Type 2 Calcium-Calmodulin-Dependent Protein Kinases - chemistry Calcium-Calmodulin-Dependent Protein Kinases - genetics Calcium-Calmodulin-Dependent Protein Kinases - metabolism CaMKII-α CaMKII-α B Cell Death Dizocilpine Maleate - pharmacology Eye and associated structures. Visual pathways and centers. Vision Fundamental and applied biological sciences. Psychology In Situ Nick-End Labeling Kinetics N-Methyl-D-aspartic acid N-Methylaspartate - pharmacology Neurons - drug effects Neurons - enzymology NMDA nuclear localization signal Nucleus Polymerase Chain Reaction Rat retina Rats Rats, Sprague-Dawley Retina - cytology Retina - enzymology Transcription, Genetic - drug effects Vertebrates: nervous system and sense organs |
| title | Calcium/calmodulin-dependent protein kinase II containing a nuclear localizing signal is altered in retinal neurons exposed to N-methyl- d-aspartate |
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