Expression of a VEGF-like Protein from Parapoxvirus ovis in the Yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe

Expression of a VEGF-like Protein from Parapoxvirus ovis in the Yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe

We report on the expression of a VEGF-like protein encoded by Parapoxvirus ovis in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. We show that a lysine residue at amino acid position 2 (K2) is an important determinant for the stability of this protein in S. cerevisiae. Replacemen...

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Veröffentlicht in:Protein expression and purification 2001-08, Vol.22 (3), p.479-483
Hauptverfasser: Kettner, Karina, Friederichs, Sonja, Schlapp, Tobias, Rödel, Gerhard
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Sprache:eng
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Affinity Labels
Hemagglutinins
Lysine
Plasmids
Recombinant Proteins - metabolism
Saccharomyces - genetics
Saccharomyces cerevisiae - genetics
Viral Proteins - metabolism
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container_issue 3
container_start_page 479
container_title Protein expression and purification
container_volume 22
creator Kettner, Karina
Friederichs, Sonja
Schlapp, Tobias
Rödel, Gerhard
description We report on the expression of a VEGF-like protein encoded by Parapoxvirus ovis in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. We show that a lysine residue at amino acid position 2 (K2) is an important determinant for the stability of this protein in S. cerevisiae. Replacement of K2 by an arginine results in stabilization of the protein. This observation suggests that this lysine may be a target for ubiquitinylation, which is a prerequisite for proteasome-mediated protein degradation. Interestingly, in S. pombe the lysine (K2) has no influence on the stability of the protein. This result indicates that the two yeast species exhibit significant differences in their protein degradation pathways.
doi_str_mv 10.1006/prep.2001.1452
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subjects Affinity Labels
Hemagglutinins
Lysine
Plasmids
Recombinant Proteins - metabolism
Saccharomyces - genetics
Saccharomyces cerevisiae - genetics
Viral Proteins - metabolism
title Expression of a VEGF-like Protein from Parapoxvirus ovis in the Yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe
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