Construction and Characterization of a Schistosoma mansoni Bacterial Artificial Chromosome Library
A bacterial artificial chromosome (BAC) library has been established from genomic DNA isolated from the trematode parasite of human, Schistosoma mansoni. This library consists of more than 21,000 recombinant clones carrying inserts in the pBeloBAC11 vector. The mean insert size was 100 kb, represent...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 2000-04, Vol.65 (2), p.87-94 |
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| container_title | Genomics (San Diego, Calif.) |
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| creator | Le Paslier, Marie-Christine Pierce, Raymond J. Merlin, Françoise Hirai, Hirohisa Wu, Wenjie Williams, David L. Johnston, David LoVerde, Philip T. Le Paslier, Denis |
| description | A bacterial artificial chromosome (BAC) library has been established from genomic DNA isolated from the trematode parasite of human, Schistosoma mansoni. This library consists of more than 21,000 recombinant clones carrying inserts in the pBeloBAC11 vector. The mean insert size was 100 kb, representing an approximate 7.95-fold genome coverage. Library screening with eight chromosome-specific or single-copy gene probes yielded between 1 and 9 positive clones, and none of those tested was absent from the library. End sequences were obtained for 93 randomly selected clones, and 37 showed sequence identity to S. mansoni sequences (ESTs, genes, or repetitive sequences). A preliminary analysis by fluorescence in situ hybridization localized 8 clones on schistosome chromosomes 1 (2 clones), 2, 3, 5, Z, and W (3 clones). This library provides a new resource for the physical mapping and sequencing of the genome of this important human pathogen. |
| doi_str_mv | 10.1006/geno.2000.6147 |
| format | Article |
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This library consists of more than 21,000 recombinant clones carrying inserts in the pBeloBAC11 vector. The mean insert size was 100 kb, representing an approximate 7.95-fold genome coverage. Library screening with eight chromosome-specific or single-copy gene probes yielded between 1 and 9 positive clones, and none of those tested was absent from the library. End sequences were obtained for 93 randomly selected clones, and 37 showed sequence identity to S. mansoni sequences (ESTs, genes, or repetitive sequences). A preliminary analysis by fluorescence in situ hybridization localized 8 clones on schistosome chromosomes 1 (2 clones), 2, 3, 5, Z, and W (3 clones). This library provides a new resource for the physical mapping and sequencing of the genome of this important human pathogen.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.2000.6147</identifier><identifier>PMID: 10783255</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; bacterial artificial chromosomes ; Base Sequence ; Biological and medical sciences ; Chromosome Mapping ; Chromosomes, Bacterial - genetics ; Cloning, Molecular ; Deoxyribonucleases, Type II Site-Specific ; DNA Probes ; DNA, Helminth - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Library ; Genes. Genome ; In Situ Hybridization, Fluorescence ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Repetitive Sequences, Nucleic Acid ; Schistosoma mansoni ; Schistosoma mansoni - genetics ; Terminal Repeat Sequences</subject><ispartof>Genomics (San Diego, Calif.), 2000-04, Vol.65 (2), p.87-94</ispartof><rights>2000 Academic Press</rights><rights>2000 INIST-CNRS</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-a249c468bdbf113c6e0a4c9a1d1adb1d6a75e1c5af2250e767492ffe24cd11673</citedby><cites>FETCH-LOGICAL-c400t-a249c468bdbf113c6e0a4c9a1d1adb1d6a75e1c5af2250e767492ffe24cd11673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/geno.2000.6147$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1361465$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10783255$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Le Paslier, Marie-Christine</creatorcontrib><creatorcontrib>Pierce, Raymond J.</creatorcontrib><creatorcontrib>Merlin, Françoise</creatorcontrib><creatorcontrib>Hirai, Hirohisa</creatorcontrib><creatorcontrib>Wu, Wenjie</creatorcontrib><creatorcontrib>Williams, David L.</creatorcontrib><creatorcontrib>Johnston, David</creatorcontrib><creatorcontrib>LoVerde, Philip T.</creatorcontrib><creatorcontrib>Le Paslier, Denis</creatorcontrib><title>Construction and Characterization of a Schistosoma mansoni Bacterial Artificial Chromosome Library</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>A bacterial artificial chromosome (BAC) library has been established from genomic DNA isolated from the trematode parasite of human, Schistosoma mansoni. This library consists of more than 21,000 recombinant clones carrying inserts in the pBeloBAC11 vector. The mean insert size was 100 kb, representing an approximate 7.95-fold genome coverage. Library screening with eight chromosome-specific or single-copy gene probes yielded between 1 and 9 positive clones, and none of those tested was absent from the library. End sequences were obtained for 93 randomly selected clones, and 37 showed sequence identity to S. mansoni sequences (ESTs, genes, or repetitive sequences). A preliminary analysis by fluorescence in situ hybridization localized 8 clones on schistosome chromosomes 1 (2 clones), 2, 3, 5, Z, and W (3 clones). This library provides a new resource for the physical mapping and sequencing of the genome of this important human pathogen.</description><subject>Animals</subject><subject>bacterial artificial chromosomes</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Bacterial - genetics</subject><subject>Cloning, Molecular</subject><subject>Deoxyribonucleases, Type II Site-Specific</subject><subject>DNA Probes</subject><subject>DNA, Helminth - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Library</subject><subject>Genes. Genome</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Schistosoma mansoni</subject><subject>Schistosoma mansoni - genetics</subject><subject>Terminal Repeat Sequences</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0EGP1CAUwHFi3Lizq1ePpgfjrSNQCvS4NuqaTLKH1TN5hYeDaWGFjon76W3tJHoxniDkxwv8CXnJ6J5RKt9-xZj2nFK6l0yoJ2THqO5qLYV8SnZUa12rVjSX5KqUb4vqGs2fkUtGlW542-7I0KdY5nyyc0ixguiq_ggZ7Iw5PMLvw-QrqO7tMZQ5lTRBNUEsKYbq3cZgrG7yHHyw67Y_5jStDqtDGDLkn8_JhYex4Ivzek2-fHj_ub-tD3cfP_U3h9oKSucauOiskHpwg2essRIpCNsBcwzcwJwE1SKzLXjOW4pKKtFx75EL6xiTqrkmb7a5Dzl9P2GZzRSKxXGEiOlUjGJU6lZ2_4VMtZx2nC9wv0GbUykZvXnIYVq-ZBg1a36z5jdrfrPmXy68Ok8-DRO6v_jWewGvzwCKhdFniDaUP65ZxsiV6Y3h0utHwGyKDRgtupDRzsal8K8n_ALlPaIv</recordid><startdate>20000415</startdate><enddate>20000415</enddate><creator>Le Paslier, Marie-Christine</creator><creator>Pierce, Raymond J.</creator><creator>Merlin, Françoise</creator><creator>Hirai, Hirohisa</creator><creator>Wu, Wenjie</creator><creator>Williams, David L.</creator><creator>Johnston, David</creator><creator>LoVerde, Philip T.</creator><creator>Le Paslier, Denis</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20000415</creationdate><title>Construction and Characterization of a Schistosoma mansoni Bacterial Artificial Chromosome Library</title><author>Le Paslier, Marie-Christine ; Pierce, Raymond J. ; Merlin, Françoise ; Hirai, Hirohisa ; Wu, Wenjie ; Williams, David L. ; Johnston, David ; LoVerde, Philip T. ; Le Paslier, Denis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-a249c468bdbf113c6e0a4c9a1d1adb1d6a75e1c5af2250e767492ffe24cd11673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>bacterial artificial chromosomes</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Bacterial - genetics</topic><topic>Cloning, Molecular</topic><topic>Deoxyribonucleases, Type II Site-Specific</topic><topic>DNA Probes</topic><topic>DNA, Helminth - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Library</topic><topic>Genes. Genome</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Schistosoma mansoni</topic><topic>Schistosoma mansoni - genetics</topic><topic>Terminal Repeat Sequences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Le Paslier, Marie-Christine</creatorcontrib><creatorcontrib>Pierce, Raymond J.</creatorcontrib><creatorcontrib>Merlin, Françoise</creatorcontrib><creatorcontrib>Hirai, Hirohisa</creatorcontrib><creatorcontrib>Wu, Wenjie</creatorcontrib><creatorcontrib>Williams, David L.</creatorcontrib><creatorcontrib>Johnston, David</creatorcontrib><creatorcontrib>LoVerde, Philip T.</creatorcontrib><creatorcontrib>Le Paslier, Denis</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Le Paslier, Marie-Christine</au><au>Pierce, Raymond J.</au><au>Merlin, Françoise</au><au>Hirai, Hirohisa</au><au>Wu, Wenjie</au><au>Williams, David L.</au><au>Johnston, David</au><au>LoVerde, Philip T.</au><au>Le Paslier, Denis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction and Characterization of a Schistosoma mansoni Bacterial Artificial Chromosome Library</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>2000-04-15</date><risdate>2000</risdate><volume>65</volume><issue>2</issue><spage>87</spage><epage>94</epage><pages>87-94</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>A bacterial artificial chromosome (BAC) library has been established from genomic DNA isolated from the trematode parasite of human, Schistosoma mansoni. This library consists of more than 21,000 recombinant clones carrying inserts in the pBeloBAC11 vector. The mean insert size was 100 kb, representing an approximate 7.95-fold genome coverage. Library screening with eight chromosome-specific or single-copy gene probes yielded between 1 and 9 positive clones, and none of those tested was absent from the library. End sequences were obtained for 93 randomly selected clones, and 37 showed sequence identity to S. mansoni sequences (ESTs, genes, or repetitive sequences). A preliminary analysis by fluorescence in situ hybridization localized 8 clones on schistosome chromosomes 1 (2 clones), 2, 3, 5, Z, and W (3 clones). This library provides a new resource for the physical mapping and sequencing of the genome of this important human pathogen.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>10783255</pmid><doi>10.1006/geno.2000.6147</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals bacterial artificial chromosomes Base Sequence Biological and medical sciences Chromosome Mapping Chromosomes, Bacterial - genetics Cloning, Molecular Deoxyribonucleases, Type II Site-Specific DNA Probes DNA, Helminth - genetics Fundamental and applied biological sciences. Psychology Gene Library Genes. Genome In Situ Hybridization, Fluorescence Molecular and cellular biology Molecular genetics Molecular Sequence Data Repetitive Sequences, Nucleic Acid Schistosoma mansoni Schistosoma mansoni - genetics Terminal Repeat Sequences |
| title | Construction and Characterization of a Schistosoma mansoni Bacterial Artificial Chromosome Library |
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