Analysis and interpretation of ligand-DNA binding isotherms

Binding studies provide information of fundamental and central importance for the complete understanding of ligand-DNA interactions. Studies of ligand binding to long natural DNA samples, to synthetic deoxypolynucleotides of simple repeating sequence, and to oligonucleotides of defined sequence are...

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Veröffentlicht in:	Methods in Enzymology 2001, Vol.340, p.3-22
1. Verfasser:	Chaires, Jonathan B
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biophysical Phenomena Biophysics Cattle Daunorubicin - chemistry Daunorubicin - metabolism DNA - chemistry DNA - metabolism DNA Adducts - chemistry DNA Adducts - metabolism In Vitro Techniques Kinetics Ligands Models, Biological Thermodynamics
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description	Binding studies provide information of fundamental and central importance for the complete understanding of ligand-DNA interactions. Studies of ligand binding to long natural DNA samples, to synthetic deoxypolynucleotides of simple repeating sequence, and to oligonucleotides of defined sequence are all needed to begin to understand the interaction in detail. Binding studies provide entry into the thermodynamics of the DNA interactions, which in turn provides great insight into the molecular forces that drive the binding process. This chapter summarizes both model-dependent and -independent approaches for the analysis and interpretation of binding isotherms, and should serve as a concise guide for handling experimental data.
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subjects	Animals Base Sequence Biophysical Phenomena Biophysics Cattle Daunorubicin - chemistry Daunorubicin - metabolism DNA - chemistry DNA - metabolism DNA Adducts - chemistry DNA Adducts - metabolism In Vitro Techniques Kinetics Ligands Models, Biological Thermodynamics
title	Analysis and interpretation of ligand-DNA binding isotherms
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