A recombinant antigen-based IgG4 ELISA for the specific and sensitive detection of Brugia malayi infection
An IgG4 ELISA based on a novel recombinant antigen was evaluated for detection of Brugia malayi infection, using 2487 sera from various institutions: 2031 samples from Universiti Sains Malaysia, 276 blinded sera from 2 other institutions in Malaysia, 140 blinded sera from India and 40 blinded sera f...
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| Veröffentlicht in: | Transactions of the Royal Society of Tropical Medicine and Hygiene 2001-05, Vol.95 (3), p.280-284 |
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| creator | Rahmah, N. Lim, B.H. Anuar, A.Khairul Shenoy, R.K. Kumaraswami, V. Hakim, S.Lokman Chotechuang, P. Kanjanopas, K. Ramachandran, C.P. |
| description | An IgG4 ELISA based on a novel recombinant antigen was evaluated for detection of
Brugia malayi infection, using 2487 sera from various institutions: 2031 samples from Universiti Sains Malaysia, 276 blinded sera from 2 other institutions in Malaysia, 140 blinded sera from India and 40 blinded sera from Thailand. These sera were from various groups of individuals, i.e., microfilaraemics, chronic patients, endemic normals, non-endemic normals and individuals with other parasitic and bacterial infections. Based on a cut-off optical density reading of 0.300, the IgG4 ELISA demonstrated specificity rates of 95·6–100%, sensitivity rates of 96–100%, positive predictive values of 75–100% and negative predictive values of 98·9–100%. These evaluation studies demonstrated the high specificity and sensitivity of this test for the detection of active
B. malayi infection. Thus, the IgG4 ELISA would be very useful as a tool in diagnosis and in elimination programmes for brugian filariasis. |
| doi_str_mv | 10.1016/S0035-9203(01)90234-2 |
| format | Article |
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Brugia malayi infection, using 2487 sera from various institutions: 2031 samples from Universiti Sains Malaysia, 276 blinded sera from 2 other institutions in Malaysia, 140 blinded sera from India and 40 blinded sera from Thailand. These sera were from various groups of individuals, i.e., microfilaraemics, chronic patients, endemic normals, non-endemic normals and individuals with other parasitic and bacterial infections. Based on a cut-off optical density reading of 0.300, the IgG4 ELISA demonstrated specificity rates of 95·6–100%, sensitivity rates of 96–100%, positive predictive values of 75–100% and negative predictive values of 98·9–100%. These evaluation studies demonstrated the high specificity and sensitivity of this test for the detection of active
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Brugia malayi infection, using 2487 sera from various institutions: 2031 samples from Universiti Sains Malaysia, 276 blinded sera from 2 other institutions in Malaysia, 140 blinded sera from India and 40 blinded sera from Thailand. These sera were from various groups of individuals, i.e., microfilaraemics, chronic patients, endemic normals, non-endemic normals and individuals with other parasitic and bacterial infections. Based on a cut-off optical density reading of 0.300, the IgG4 ELISA demonstrated specificity rates of 95·6–100%, sensitivity rates of 96–100%, positive predictive values of 75–100% and negative predictive values of 98·9–100%. These evaluation studies demonstrated the high specificity and sensitivity of this test for the detection of active
B. malayi infection. Thus, the IgG4 ELISA would be very useful as a tool in diagnosis and in elimination programmes for brugian filariasis.</description><subject>Animals</subject><subject>Antigens, Helminth - blood</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Brugia malayi</subject><subject>Brugia malayi - immunology</subject><subject>diagnosis</subject><subject>Diseases caused by nematodes</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - standards</subject><subject>evaluation</subject><subject>Filariases</subject><subject>filariasis</subject><subject>Filariasis - diagnosis</subject><subject>Filariasis - immunology</subject><subject>Follow-Up Studies</subject><subject>Helminthic diseases</subject><subject>Humans</subject><subject>IgG4</subject><subject>Immunoglobulin G - blood</subject><subject>India</subject><subject>Infectious diseases</subject><subject>Lymphatic filariases</subject><subject>Malaysia</subject><subject>Medical sciences</subject><subject>Parasitic diseases</subject><subject>recombinant antigen</subject><subject>Recombinant Proteins</subject><subject>Sensitivity and Specificity</subject><subject>Thailand</subject><subject>Tropical medicine</subject><issn>0035-9203</issn><issn>1878-3503</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1uEzEUhS0EoqHwCCAvEIKFwT_jGXtVpVFJIgWxKEiIjeXxXAeXyUywZ6r27et0ogISEgvLi_ude3TOReglo-8ZZeWHS0qFJJpT8Zayd5pyURD-CM2YqhQRkorHaPaAnKBnKV1RyiWT-ik6YazQVOtqhq7mOILrd3XobDfg_MIWOlLbBA1eb5cFvtisL-fY9xEPPwCnPbjgg8tkgxN0KQzhGnADA7gh9B3uPT6P4zZYvLOtvQ04dH4aPUdPvG0TvDj-p-jrx4svixXZfF6uF_MNcSUrB-KZrBzYxlNXKsZ5DUo78KArrvJIN7UTXuRQSlpVQk21y3FqSn3leOWlOEVvpr372P8aIQ1mF5KDtrUd9GMyFaOlLFSRQTmBLvYpRfBmH8POxlvDqDmUbO5LNocGDWXmvmTDs-7V0WCsd9D8Vh1bzcDrI2CTs62PtnMh_bFdcyVVxuiE9eP-39bkL2tysCaTJKQBbh5ENv40ZSUqaVbfvhu6EOd6xZbmU-bPJh5y4dcBokkuQOegCfnsg2n68J-wd5e3tNU</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>Rahmah, N.</creator><creator>Lim, B.H.</creator><creator>Anuar, A.Khairul</creator><creator>Shenoy, R.K.</creator><creator>Kumaraswami, V.</creator><creator>Hakim, S.Lokman</creator><creator>Chotechuang, P.</creator><creator>Kanjanopas, K.</creator><creator>Ramachandran, C.P.</creator><general>Elsevier Ltd</general><general>Royal Society of Tropical Medicine and Hygiene</general><general>Elsevier</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010501</creationdate><title>A recombinant antigen-based IgG4 ELISA for the specific and sensitive detection of Brugia malayi infection</title><author>Rahmah, N. ; Lim, B.H. ; Anuar, A.Khairul ; Shenoy, R.K. ; Kumaraswami, V. ; Hakim, S.Lokman ; Chotechuang, P. ; Kanjanopas, K. ; Ramachandran, C.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c616t-f157ceadf0c68122be89cefe97281579dbc3f320385a86eb09c114b00f7c27f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Antigens, Helminth - blood</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Brugia malayi</topic><topic>Brugia malayi - immunology</topic><topic>diagnosis</topic><topic>Diseases caused by nematodes</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - standards</topic><topic>evaluation</topic><topic>Filariases</topic><topic>filariasis</topic><topic>Filariasis - diagnosis</topic><topic>Filariasis - immunology</topic><topic>Follow-Up Studies</topic><topic>Helminthic diseases</topic><topic>Humans</topic><topic>IgG4</topic><topic>Immunoglobulin G - blood</topic><topic>India</topic><topic>Infectious diseases</topic><topic>Lymphatic filariases</topic><topic>Malaysia</topic><topic>Medical sciences</topic><topic>Parasitic diseases</topic><topic>recombinant antigen</topic><topic>Recombinant Proteins</topic><topic>Sensitivity and Specificity</topic><topic>Thailand</topic><topic>Tropical medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rahmah, N.</creatorcontrib><creatorcontrib>Lim, B.H.</creatorcontrib><creatorcontrib>Anuar, A.Khairul</creatorcontrib><creatorcontrib>Shenoy, R.K.</creatorcontrib><creatorcontrib>Kumaraswami, V.</creatorcontrib><creatorcontrib>Hakim, S.Lokman</creatorcontrib><creatorcontrib>Chotechuang, P.</creatorcontrib><creatorcontrib>Kanjanopas, K.</creatorcontrib><creatorcontrib>Ramachandran, C.P.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rahmah, N.</au><au>Lim, B.H.</au><au>Anuar, A.Khairul</au><au>Shenoy, R.K.</au><au>Kumaraswami, V.</au><au>Hakim, S.Lokman</au><au>Chotechuang, P.</au><au>Kanjanopas, K.</au><au>Ramachandran, C.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A recombinant antigen-based IgG4 ELISA for the specific and sensitive detection of Brugia malayi infection</atitle><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle><stitle>Trans R Soc Trop Med Hyg</stitle><addtitle>Trans R Soc Trop Med Hyg</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>95</volume><issue>3</issue><spage>280</spage><epage>284</epage><pages>280-284</pages><issn>0035-9203</issn><eissn>1878-3503</eissn><coden>TRSTAZ</coden><abstract>An IgG4 ELISA based on a novel recombinant antigen was evaluated for detection of
Brugia malayi infection, using 2487 sera from various institutions: 2031 samples from Universiti Sains Malaysia, 276 blinded sera from 2 other institutions in Malaysia, 140 blinded sera from India and 40 blinded sera from Thailand. These sera were from various groups of individuals, i.e., microfilaraemics, chronic patients, endemic normals, non-endemic normals and individuals with other parasitic and bacterial infections. Based on a cut-off optical density reading of 0.300, the IgG4 ELISA demonstrated specificity rates of 95·6–100%, sensitivity rates of 96–100%, positive predictive values of 75–100% and negative predictive values of 98·9–100%. These evaluation studies demonstrated the high specificity and sensitivity of this test for the detection of active
B. malayi infection. Thus, the IgG4 ELISA would be very useful as a tool in diagnosis and in elimination programmes for brugian filariasis.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11490997</pmid><doi>10.1016/S0035-9203(01)90234-2</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0035-9203 |
| ispartof | Transactions of the Royal Society of Tropical Medicine and Hygiene, 2001-05, Vol.95 (3), p.280-284 |
| issn | 0035-9203 1878-3503 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Animals Antigens, Helminth - blood Biological and medical sciences Blotting, Western Brugia malayi Brugia malayi - immunology diagnosis Diseases caused by nematodes ELISA Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - standards evaluation Filariases filariasis Filariasis - diagnosis Filariasis - immunology Follow-Up Studies Helminthic diseases Humans IgG4 Immunoglobulin G - blood India Infectious diseases Lymphatic filariases Malaysia Medical sciences Parasitic diseases recombinant antigen Recombinant Proteins Sensitivity and Specificity Thailand Tropical medicine |
| title | A recombinant antigen-based IgG4 ELISA for the specific and sensitive detection of Brugia malayi infection |
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