Fluorescence spectroscopy of normal mouse skin exposed to 5-aminolaevulinic acid and red light
Photobleaching and phototransformation of protoporphyrin IX (PpIX) was investigated in normal mouse skin. The PpIX was induced by topical application of 5-aminolaevulinic acid (ALA). Exposure to laser light (635 nm) caused photobleaching of PpIX fluorescence and formation of fluorescent products. An...
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| Veröffentlicht in: | Journal of photochemistry and photobiology. B, Biology Biology, 2001-08, Vol.61 (1), p.78-86 |
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| container_title | Journal of photochemistry and photobiology. B, Biology |
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| creator | Juzenas, Petras Iani, Vladimir Bagdonas, Saulius Rotomskis, Ricardas Moan, Johan |
| description | Photobleaching and phototransformation of protoporphyrin IX (PpIX) was investigated in normal mouse skin. The PpIX was induced by topical application of 5-aminolaevulinic acid (ALA). Exposure to laser light (635 nm) caused photobleaching of PpIX fluorescence and formation of fluorescent products. Analysis of the fluorescence spectra revealed appearance of new fluorescent photoproducts during light exposure. The main photoproduct, supposedly chlorin-type photoprotoporphyrin (PPp), exhibited fluorescence with an emission maximum at 675 nm. The other products exhibited main fluorescence peaks at around 588 and 623 nm that can presumably be attributed to an endogenous metallo-porphyrin and water-soluble porphyrin(s), respectively. Our results indicate that light exposure causes alterations in the enzymatic pathway of PpIX synthesis from ALA and leads to accumulation of intermediate water-soluble porphyrins. ALA-induced porphyrins are transported away from the treated area and partly deposited in remote skin sites. |
| doi_str_mv | 10.1016/S1011-1344(01)00149-X |
| format | Article |
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The PpIX was induced by topical application of 5-aminolaevulinic acid (ALA). Exposure to laser light (635 nm) caused photobleaching of PpIX fluorescence and formation of fluorescent products. Analysis of the fluorescence spectra revealed appearance of new fluorescent photoproducts during light exposure. The main photoproduct, supposedly chlorin-type photoprotoporphyrin (PPp), exhibited fluorescence with an emission maximum at 675 nm. The other products exhibited main fluorescence peaks at around 588 and 623 nm that can presumably be attributed to an endogenous metallo-porphyrin and water-soluble porphyrin(s), respectively. Our results indicate that light exposure causes alterations in the enzymatic pathway of PpIX synthesis from ALA and leads to accumulation of intermediate water-soluble porphyrins. ALA-induced porphyrins are transported away from the treated area and partly deposited in remote skin sites.</description><identifier>ISSN: 1011-1344</identifier><identifier>EISSN: 1873-2682</identifier><identifier>DOI: 10.1016/S1011-1344(01)00149-X</identifier><identifier>PMID: 11485851</identifier><language>eng</language><publisher>Switzerland: Elsevier B.V</publisher><subject>Aminolevulinic Acid - pharmacology ; Animals ; Female ; Fluorescence ; Fluorescence spectroscopy ; Kinetics ; Light ; Mice ; Mice, Inbred BALB C ; Photobleaching ; Photodynamic therapy ; Photoproduct ; Photoprotoporphyrin ; Photosensitizing Agents - pharmacology ; Protoporphyrin IX ; Protoporphyrins - metabolism ; Skin - drug effects ; Skin - metabolism ; Spectrometry, Fluorescence - methods ; Water-soluble porphyrins</subject><ispartof>Journal of photochemistry and photobiology. B, Biology, 2001-08, Vol.61 (1), p.78-86</ispartof><rights>2001 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-4ca186d46231f6f8e6dafb6df44cea68f9406293098f062af5871a61feead6b43</citedby><cites>FETCH-LOGICAL-c361t-4ca186d46231f6f8e6dafb6df44cea68f9406293098f062af5871a61feead6b43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1011-1344(01)00149-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11485851$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Juzenas, Petras</creatorcontrib><creatorcontrib>Iani, Vladimir</creatorcontrib><creatorcontrib>Bagdonas, Saulius</creatorcontrib><creatorcontrib>Rotomskis, Ricardas</creatorcontrib><creatorcontrib>Moan, Johan</creatorcontrib><title>Fluorescence spectroscopy of normal mouse skin exposed to 5-aminolaevulinic acid and red light</title><title>Journal of photochemistry and photobiology. B, Biology</title><addtitle>J Photochem Photobiol B</addtitle><description>Photobleaching and phototransformation of protoporphyrin IX (PpIX) was investigated in normal mouse skin. The PpIX was induced by topical application of 5-aminolaevulinic acid (ALA). Exposure to laser light (635 nm) caused photobleaching of PpIX fluorescence and formation of fluorescent products. Analysis of the fluorescence spectra revealed appearance of new fluorescent photoproducts during light exposure. The main photoproduct, supposedly chlorin-type photoprotoporphyrin (PPp), exhibited fluorescence with an emission maximum at 675 nm. The other products exhibited main fluorescence peaks at around 588 and 623 nm that can presumably be attributed to an endogenous metallo-porphyrin and water-soluble porphyrin(s), respectively. Our results indicate that light exposure causes alterations in the enzymatic pathway of PpIX synthesis from ALA and leads to accumulation of intermediate water-soluble porphyrins. ALA-induced porphyrins are transported away from the treated area and partly deposited in remote skin sites.</description><subject>Aminolevulinic Acid - pharmacology</subject><subject>Animals</subject><subject>Female</subject><subject>Fluorescence</subject><subject>Fluorescence spectroscopy</subject><subject>Kinetics</subject><subject>Light</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Photobleaching</subject><subject>Photodynamic therapy</subject><subject>Photoproduct</subject><subject>Photoprotoporphyrin</subject><subject>Photosensitizing Agents - pharmacology</subject><subject>Protoporphyrin IX</subject><subject>Protoporphyrins - metabolism</subject><subject>Skin - drug effects</subject><subject>Skin - metabolism</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Water-soluble porphyrins</subject><issn>1011-1344</issn><issn>1873-2682</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P5SAUhokZ4_dP0LCa6KLKaSmXribG-JWYuFATVxIuHGbQtnSgNfrv5XqvcSkLOAnP4fA-hOwDOwYG4uQu71BAxfkhgyPGgDfF4xrZAjmrilLI8leuv5BNsp3SM8urFrMNsgnAZS1r2CJPF-0UIiaDvUGaBjRjDMmE4Z0GR_sQO93SLkwpX774nuLbEBJaOgZaF7rzfWg1vk6t772h2nhLdW9pzETr__4bd8m6023CvdW5Qx4uzu_Proqb28vrs9ObwlQCxoIbDVJYLsoKnHAShdVuLqzj3KAW0jWcibKpWCNdLrSr5Qy0AIeorZjzaof8Xr47xPB_wjSqzudMbat7zJ9XM2B1U0qWwXoJmhwzRXRqiL7T8V0BUwux6lOsWlhTDNSnWPWY-w5WA6Z5h_a7a2UyA3-WAOaYrx6jSsYvpFofs1Rlg_9hxAcRYIlU</recordid><startdate>20010815</startdate><enddate>20010815</enddate><creator>Juzenas, Petras</creator><creator>Iani, Vladimir</creator><creator>Bagdonas, Saulius</creator><creator>Rotomskis, Ricardas</creator><creator>Moan, Johan</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010815</creationdate><title>Fluorescence spectroscopy of normal mouse skin exposed to 5-aminolaevulinic acid and red light</title><author>Juzenas, Petras ; Iani, Vladimir ; Bagdonas, Saulius ; Rotomskis, Ricardas ; Moan, Johan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-4ca186d46231f6f8e6dafb6df44cea68f9406293098f062af5871a61feead6b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Aminolevulinic Acid - pharmacology</topic><topic>Animals</topic><topic>Female</topic><topic>Fluorescence</topic><topic>Fluorescence spectroscopy</topic><topic>Kinetics</topic><topic>Light</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Photobleaching</topic><topic>Photodynamic therapy</topic><topic>Photoproduct</topic><topic>Photoprotoporphyrin</topic><topic>Photosensitizing Agents - pharmacology</topic><topic>Protoporphyrin IX</topic><topic>Protoporphyrins - metabolism</topic><topic>Skin - drug effects</topic><topic>Skin - metabolism</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Water-soluble porphyrins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Juzenas, Petras</creatorcontrib><creatorcontrib>Iani, Vladimir</creatorcontrib><creatorcontrib>Bagdonas, Saulius</creatorcontrib><creatorcontrib>Rotomskis, Ricardas</creatorcontrib><creatorcontrib>Moan, Johan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Juzenas, Petras</au><au>Iani, Vladimir</au><au>Bagdonas, Saulius</au><au>Rotomskis, Ricardas</au><au>Moan, Johan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescence spectroscopy of normal mouse skin exposed to 5-aminolaevulinic acid and red light</atitle><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle><addtitle>J Photochem Photobiol B</addtitle><date>2001-08-15</date><risdate>2001</risdate><volume>61</volume><issue>1</issue><spage>78</spage><epage>86</epage><pages>78-86</pages><issn>1011-1344</issn><eissn>1873-2682</eissn><abstract>Photobleaching and phototransformation of protoporphyrin IX (PpIX) was investigated in normal mouse skin. The PpIX was induced by topical application of 5-aminolaevulinic acid (ALA). Exposure to laser light (635 nm) caused photobleaching of PpIX fluorescence and formation of fluorescent products. Analysis of the fluorescence spectra revealed appearance of new fluorescent photoproducts during light exposure. The main photoproduct, supposedly chlorin-type photoprotoporphyrin (PPp), exhibited fluorescence with an emission maximum at 675 nm. The other products exhibited main fluorescence peaks at around 588 and 623 nm that can presumably be attributed to an endogenous metallo-porphyrin and water-soluble porphyrin(s), respectively. Our results indicate that light exposure causes alterations in the enzymatic pathway of PpIX synthesis from ALA and leads to accumulation of intermediate water-soluble porphyrins. ALA-induced porphyrins are transported away from the treated area and partly deposited in remote skin sites.</abstract><cop>Switzerland</cop><pub>Elsevier B.V</pub><pmid>11485851</pmid><doi>10.1016/S1011-1344(01)00149-X</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of photochemistry and photobiology. B, Biology, 2001-08, Vol.61 (1), p.78-86 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aminolevulinic Acid - pharmacology Animals Female Fluorescence Fluorescence spectroscopy Kinetics Light Mice Mice, Inbred BALB C Photobleaching Photodynamic therapy Photoproduct Photoprotoporphyrin Photosensitizing Agents - pharmacology Protoporphyrin IX Protoporphyrins - metabolism Skin - drug effects Skin - metabolism Spectrometry, Fluorescence - methods Water-soluble porphyrins |
| title | Fluorescence spectroscopy of normal mouse skin exposed to 5-aminolaevulinic acid and red light |
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