The application of ethanol-extracted Gloriosa superba for metaphase chromosome preparation in mosquitos
The application of ethanol-extracted Gloriosa superba for metaphase chromosome preparation in adult and 4th larva Aedes aegypti revealed that 0.5-8% ethanol-extracted Gl. superba solution could be used instead of 1% colchicine in Hanks' balanced salt solution. For adult mosquitos, the metaphase...
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| Veröffentlicht in: | Southeast Asian journal of tropical medicine and public health 2001-03, Vol.32 (1), p.76-82 |
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| creator | CHOOCHOTE, Wej PITASAWAT, Benjawan JITPAKDI, Atchariya RATTANACHANPICHAI, Eumporn RIYONG, Doungrat LEEMINGSAWAT, Somjai WONGKAMCHAI, Sirichit |
| description | The application of ethanol-extracted Gloriosa superba for metaphase chromosome preparation in adult and 4th larva Aedes aegypti revealed that 0.5-8% ethanol-extracted Gl. superba solution could be used instead of 1% colchicine in Hanks' balanced salt solution. For adult mosquitos, the metaphase rates and average number of metaphase chromosomes per positive mosquito after intrathoracic inoculation with 1-2% ethanol-extracted Gl. superba solution were 100% and 11.8 (2-16) -12.6 (3-28) in females, and 80-90% and 16.5 (1-52) - 29.89 (1-72) in males, whereas the inoculation with 1% colchicine solution yielded 80% and 50% metaphase rates, and 18.25 (1-40) and 16.5 (2-53) average number of metaphase chromosomes per positive mosquito in females and males, respectively. For 4th stage larvae, the metaphase rates and average number of metaphase chromosomes per positive mosquito after incubation with 0.5-8% ethanol-extracted Gl. superba solution were 90-100% and 14.42 (1-65) - 64 (19-137), while incubation with 1% colchicine solution yield 100% metaphase rate and 10.9 (7-15) average number of metaphase chromosomes per positive mosquito. |
| format | Article |
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For adult mosquitos, the metaphase rates and average number of metaphase chromosomes per positive mosquito after intrathoracic inoculation with 1-2% ethanol-extracted Gl. superba solution were 100% and 11.8 (2-16) -12.6 (3-28) in females, and 80-90% and 16.5 (1-52) - 29.89 (1-72) in males, whereas the inoculation with 1% colchicine solution yielded 80% and 50% metaphase rates, and 18.25 (1-40) and 16.5 (2-53) average number of metaphase chromosomes per positive mosquito in females and males, respectively. For 4th stage larvae, the metaphase rates and average number of metaphase chromosomes per positive mosquito after incubation with 0.5-8% ethanol-extracted Gl. superba solution were 90-100% and 14.42 (1-65) - 64 (19-137), while incubation with 1% colchicine solution yield 100% metaphase rate and 10.9 (7-15) average number of metaphase chromosomes per positive mosquito.</description><identifier>ISSN: 0125-1562</identifier><identifier>PMID: 11485100</identifier><identifier>CODEN: SJTMAK</identifier><language>eng</language><publisher>Bangkok: Southeast Asian Ministers of Education Organization, Regional Tropical Medicine and Public Health Network</publisher><subject>Animals ; Biological and medical sciences ; Chromosomes ; Culicidae - genetics ; Culicidae - growth & development ; Female ; Fundamental and applied biological sciences. Psychology ; Karyotyping ; Larva - ultrastructure ; Magnoliopsida - chemistry ; Male ; Medically important nuisances and vectors, pests of stored products and materials: population survey and control ; Metaphase ; Plant Extracts - chemistry ; Tropical medicine ; Vectors. 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For adult mosquitos, the metaphase rates and average number of metaphase chromosomes per positive mosquito after intrathoracic inoculation with 1-2% ethanol-extracted Gl. superba solution were 100% and 11.8 (2-16) -12.6 (3-28) in females, and 80-90% and 16.5 (1-52) - 29.89 (1-72) in males, whereas the inoculation with 1% colchicine solution yielded 80% and 50% metaphase rates, and 18.25 (1-40) and 16.5 (2-53) average number of metaphase chromosomes per positive mosquito in females and males, respectively. For 4th stage larvae, the metaphase rates and average number of metaphase chromosomes per positive mosquito after incubation with 0.5-8% ethanol-extracted Gl. superba solution were 90-100% and 14.42 (1-65) - 64 (19-137), while incubation with 1% colchicine solution yield 100% metaphase rate and 10.9 (7-15) average number of metaphase chromosomes per positive mosquito.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromosomes</subject><subject>Culicidae - genetics</subject><subject>Culicidae - growth & development</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Karyotyping</subject><subject>Larva - ultrastructure</subject><subject>Magnoliopsida - chemistry</subject><subject>Male</subject><subject>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</subject><subject>Metaphase</subject><subject>Plant Extracts - chemistry</subject><subject>Tropical medicine</subject><subject>Vectors. 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Psychology</topic><topic>Karyotyping</topic><topic>Larva - ultrastructure</topic><topic>Magnoliopsida - chemistry</topic><topic>Male</topic><topic>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</topic><topic>Metaphase</topic><topic>Plant Extracts - chemistry</topic><topic>Tropical medicine</topic><topic>Vectors. Intermediate hosts</topic><toplevel>online_resources</toplevel><creatorcontrib>CHOOCHOTE, Wej</creatorcontrib><creatorcontrib>PITASAWAT, Benjawan</creatorcontrib><creatorcontrib>JITPAKDI, Atchariya</creatorcontrib><creatorcontrib>RATTANACHANPICHAI, Eumporn</creatorcontrib><creatorcontrib>RIYONG, Doungrat</creatorcontrib><creatorcontrib>LEEMINGSAWAT, Somjai</creatorcontrib><creatorcontrib>WONGKAMCHAI, Sirichit</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Southeast Asian journal of tropical medicine and public health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHOOCHOTE, Wej</au><au>PITASAWAT, Benjawan</au><au>JITPAKDI, Atchariya</au><au>RATTANACHANPICHAI, Eumporn</au><au>RIYONG, Doungrat</au><au>LEEMINGSAWAT, Somjai</au><au>WONGKAMCHAI, Sirichit</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The application of ethanol-extracted Gloriosa superba for metaphase chromosome preparation in mosquitos</atitle><jtitle>Southeast Asian journal of tropical medicine and public health</jtitle><addtitle>Southeast Asian J Trop Med Public Health</addtitle><date>2001-03</date><risdate>2001</risdate><volume>32</volume><issue>1</issue><spage>76</spage><epage>82</epage><pages>76-82</pages><issn>0125-1562</issn><coden>SJTMAK</coden><abstract>The application of ethanol-extracted Gloriosa superba for metaphase chromosome preparation in adult and 4th larva Aedes aegypti revealed that 0.5-8% ethanol-extracted Gl. superba solution could be used instead of 1% colchicine in Hanks' balanced salt solution. For adult mosquitos, the metaphase rates and average number of metaphase chromosomes per positive mosquito after intrathoracic inoculation with 1-2% ethanol-extracted Gl. superba solution were 100% and 11.8 (2-16) -12.6 (3-28) in females, and 80-90% and 16.5 (1-52) - 29.89 (1-72) in males, whereas the inoculation with 1% colchicine solution yielded 80% and 50% metaphase rates, and 18.25 (1-40) and 16.5 (2-53) average number of metaphase chromosomes per positive mosquito in females and males, respectively. For 4th stage larvae, the metaphase rates and average number of metaphase chromosomes per positive mosquito after incubation with 0.5-8% ethanol-extracted Gl. superba solution were 90-100% and 14.42 (1-65) - 64 (19-137), while incubation with 1% colchicine solution yield 100% metaphase rate and 10.9 (7-15) average number of metaphase chromosomes per positive mosquito.</abstract><cop>Bangkok</cop><pub>Southeast Asian Ministers of Education Organization, Regional Tropical Medicine and Public Health Network</pub><pmid>11485100</pmid><tpages>7</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Chromosomes Culicidae - genetics Culicidae - growth & development Female Fundamental and applied biological sciences. Psychology Karyotyping Larva - ultrastructure Magnoliopsida - chemistry Male Medically important nuisances and vectors, pests of stored products and materials: population survey and control Metaphase Plant Extracts - chemistry Tropical medicine Vectors. Intermediate hosts |
| title | The application of ethanol-extracted Gloriosa superba for metaphase chromosome preparation in mosquitos |
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