Human Aromatase in High Yield and Purity by Perfusion Chromatography and Its Characterization by Difference Spectroscopy and Mass Spectrometry

Expression of human cytochrome P450 aromatase (CYP19A1, aromatase) was accomplished at a high level using a baculovirus expression system in an insect cell suspension culture. Using the relatively new chromatographic technique of perfusion chromatography, a very rapid procedure for purification of t...

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Veröffentlicht in:	Protein expression and purification 2001-08, Vol.22 (3), p.443-454
Hauptverfasser:	Gartner, Carlos A., Thompson, Stella J., Rettie, Allan E., Nelson, Sidney D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals aromatase Aromatase - genetics Aromatase - isolation & purification Aromatase - metabolism Baculoviridae - genetics Chromatography - methods cytochrome P450 glycosylation Humans Insecta - genetics Kinetics MALDI-TOF Mass Spectrometry perfusion chromatography purification Recombinant Proteins - analysis Recombinant Proteins - metabolism Spectrum Analysis - methods
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