A novel method of isolation of a bone-morphogenetic-protein-like protein from ossein
A new bone‐morphogenetic‐protein (BMP)‐like protein has been isolated through a new protocol from a novel source, ossein. The BMP‐like protein was hydrophilic and characterized through Fourier‐transform IR studies, SDS/PAGE and coupled with a neutral binder, hydroxypropylmethylcellulose (HPMC) for c...
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| Veröffentlicht in: | Biotechnology and applied biochemistry 2001-08, Vol.34 (1), p.33-36 |
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| container_title | Biotechnology and applied biochemistry |
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| creator | Mythili, J. Padmavathy, S. Chandrakasan, Gowri |
| description | A new bone‐morphogenetic‐protein (BMP)‐like protein has been isolated through a new protocol from a novel source, ossein. The BMP‐like protein was hydrophilic and characterized through Fourier‐transform IR studies, SDS/PAGE and coupled with a neutral binder, hydroxypropylmethylcellulose (HPMC) for control release. The IR spectrum of the protein showed peaks in tandem with BMP from bone matrix, and its molecular mass was in the range 18–21 kDa. Sustained release from the surface of HPMC was achieved for a period of 3 days. |
| doi_str_mv | 10.1042/BA20000061 |
| format | Article |
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The BMP‐like protein was hydrophilic and characterized through Fourier‐transform IR studies, SDS/PAGE and coupled with a neutral binder, hydroxypropylmethylcellulose (HPMC) for control release. The IR spectrum of the protein showed peaks in tandem with BMP from bone matrix, and its molecular mass was in the range 18–21 kDa. 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The BMP‐like protein was hydrophilic and characterized through Fourier‐transform IR studies, SDS/PAGE and coupled with a neutral binder, hydroxypropylmethylcellulose (HPMC) for control release. The IR spectrum of the protein showed peaks in tandem with BMP from bone matrix, and its molecular mass was in the range 18–21 kDa. Sustained release from the surface of HPMC was achieved for a period of 3 days.</description><subject>Biochemistry - methods</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Bone and Bones - chemistry</subject><subject>Bone Morphogenetic Proteins - chemistry</subject><subject>Bone Morphogenetic Proteins - isolation & purification</subject><subject>bone-graft device</subject><subject>bovine</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Health. Pharmaceutical industry</subject><subject>hydroxypropylmethylcellulose</subject><subject>Hypromellose Derivatives</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Methylcellulose - analogs & derivatives</subject><subject>Methylcellulose - chemistry</subject><subject>Miscellaneous</subject><subject>ossein</subject><subject>osteogenin</subject><subject>Protein Binding</subject><subject>Spectroscopy, Fourier Transform Infrared - methods</subject><subject>Time Factors</subject><issn>0885-4513</issn><issn>1470-8744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vEzEQhi0EoqFw4QegvcChkou_dm0f04gU1AguQXCzvOsxNd1dp_YG2n-Po6zIDXwYz0jPO2O_g9BrSi4pEez91ZKRw2noE7SgQhKspBBP0YIoVWNRU36GXuT8syBKKvYcnVEqFKc1W6DtshrjL-irAabb6Kroq5Bjb6cQx0NhqzaOgIeYdrfxB4wwhQ7vUpwgjLgPd1DNReVTHKqYc8lfomfe9hlezfc5-rr-sF19xJsv159Wyw3uBKMCSy0cc6JpQdSsbhvugfqWWG29Zr5x1DvnZKdBaKqAEmVr5qyUmoETnWz5OXp37FvecL-HPJkh5A763o4Q99lISuqmhP-CVHEhtGgKeHEEu1S-ksCbXQqDTY-GEnMw25zMLvCbueu-HcCd0NndArydAZs72_tkxy7kEyeI1mUjhbs8cr9DD4__GFnSK81FEeCjIOQJHv4KbLozjeSyNt8-X5vt-mZzs_6-Mpz_AXO5oxo</recordid><startdate>200108</startdate><enddate>200108</enddate><creator>Mythili, J.</creator><creator>Padmavathy, S.</creator><creator>Chandrakasan, Gowri</creator><general>Blackwell Publishing Ltd</general><general>Portland Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200108</creationdate><title>A novel method of isolation of a bone-morphogenetic-protein-like protein from ossein</title><author>Mythili, J. ; Padmavathy, S. ; Chandrakasan, Gowri</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4214-794d2d46be4525b63fe1fb0a9af92f6d1fddd7c9e4918e108a52da7792ed4c7b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Biochemistry - methods</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Bone and Bones - chemistry</topic><topic>Bone Morphogenetic Proteins - chemistry</topic><topic>Bone Morphogenetic Proteins - isolation & purification</topic><topic>bone-graft device</topic><topic>bovine</topic><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Health. Pharmaceutical industry</topic><topic>hydroxypropylmethylcellulose</topic><topic>Hypromellose Derivatives</topic><topic>Industrial applications and implications. 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The BMP‐like protein was hydrophilic and characterized through Fourier‐transform IR studies, SDS/PAGE and coupled with a neutral binder, hydroxypropylmethylcellulose (HPMC) for control release. The IR spectrum of the protein showed peaks in tandem with BMP from bone matrix, and its molecular mass was in the range 18–21 kDa. Sustained release from the surface of HPMC was achieved for a period of 3 days.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>11483152</pmid><doi>10.1042/BA20000061</doi><tpages>4</tpages></addata></record> |
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| ispartof | Biotechnology and applied biochemistry, 2001-08, Vol.34 (1), p.33-36 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Biochemistry - methods Biocompatible Materials - chemistry Biological and medical sciences Biotechnology Bone and Bones - chemistry Bone Morphogenetic Proteins - chemistry Bone Morphogenetic Proteins - isolation & purification bone-graft device bovine Electrophoresis, Polyacrylamide Gel - methods Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry hydroxypropylmethylcellulose Hypromellose Derivatives Industrial applications and implications. Economical aspects Methylcellulose - analogs & derivatives Methylcellulose - chemistry Miscellaneous ossein osteogenin Protein Binding Spectroscopy, Fourier Transform Infrared - methods Time Factors |
| title | A novel method of isolation of a bone-morphogenetic-protein-like protein from ossein |
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