Analysis of Moraxella catarrhalis by DNA Typing: Evidence for a Distinct Subpopulation Associated with Virulence Traits
Two DNA typing methods, probe-generated restriction fragment length polymorphism analysis and single-adapter amplified fragment length polymorphism analysis, were used to study the genetic relationships among 90 Moraxella catarrhalis strains. Both methods were found to be highly concordant, generati...
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| Veröffentlicht in: | The Journal of infectious diseases 2000-04, Vol.181 (4), p.1376-1387 |
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| container_title | The Journal of infectious diseases |
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| creator | Bootsma, Hester J. van der Heide, Han G. J. van de Pas, Simone Schouls, Leo M. Mooi, Frits R. |
| description | Two DNA typing methods, probe-generated restriction fragment length polymorphism analysis and single-adapter amplified fragment length polymorphism analysis, were used to study the genetic relationships among 90 Moraxella catarrhalis strains. Both methods were found to be highly concordant, generating a dendrogram with 2 main branches. The division of the M. catarrhalis population into 2 subspecies was supported by analysis of the 16S rRNA sequences. Both β-lactamase—positive and β-lactamase—negative strains were found in all main branches, suggesting horizontal transfer of the β-lactamase gene. In contrast, 2 virulence traits, complement resistance and adherence to epithelial cells, were strongly associated with 1 of the 2 subspecies. The branch depth suggested that complement-resistant adherent strains diverged from a common ancestor more recently than did complement-sensitive nonadherent strains. These findings suggest the existence of subpopulations of M. catarrhalis that differ in virulence, and they may have implications for vaccine development. |
| doi_str_mv | 10.1086/315374 |
| format | Article |
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The branch depth suggested that complement-resistant adherent strains diverged from a common ancestor more recently than did complement-sensitive nonadherent strains. These findings suggest the existence of subpopulations of M. catarrhalis that differ in virulence, and they may have implications for vaccine development.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/315374</identifier><identifier>PMID: 10762569</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Adult ; Antigens, Bacterial - genetics ; Antigens, Surface - genetics ; Bacterial Outer Membrane Proteins - genetics ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Carrier State ; Child ; DNA ; DNA fingerprinting ; Epithelial cells ; Fundamental and applied biological sciences. 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J.</creatorcontrib><creatorcontrib>van de Pas, Simone</creatorcontrib><creatorcontrib>Schouls, Leo M.</creatorcontrib><creatorcontrib>Mooi, Frits R.</creatorcontrib><title>Analysis of Moraxella catarrhalis by DNA Typing: Evidence for a Distinct Subpopulation Associated with Virulence Traits</title><title>The Journal of infectious diseases</title><addtitle>The Journal of Infectious Diseases</addtitle><description>Two DNA typing methods, probe-generated restriction fragment length polymorphism analysis and single-adapter amplified fragment length polymorphism analysis, were used to study the genetic relationships among 90 Moraxella catarrhalis strains. Both methods were found to be highly concordant, generating a dendrogram with 2 main branches. The division of the M. catarrhalis population into 2 subspecies was supported by analysis of the 16S rRNA sequences. Both β-lactamase—positive and β-lactamase—negative strains were found in all main branches, suggesting horizontal transfer of the β-lactamase gene. In contrast, 2 virulence traits, complement resistance and adherence to epithelial cells, were strongly associated with 1 of the 2 subspecies. The branch depth suggested that complement-resistant adherent strains diverged from a common ancestor more recently than did complement-sensitive nonadherent strains. These findings suggest the existence of subpopulations of M. catarrhalis that differ in virulence, and they may have implications for vaccine development.</description><subject>Adult</subject><subject>Antigens, Bacterial - genetics</subject><subject>Antigens, Surface - genetics</subject><subject>Bacterial Outer Membrane Proteins - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Carrier State</subject><subject>Child</subject><subject>DNA</subject><subject>DNA fingerprinting</subject><subject>Epithelial cells</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Gels</topic><topic>Humans</topic><topic>Laryngitis - microbiology</topic><topic>Lung Diseases, Obstructive - genetics</topic><topic>Lung Diseases, Obstructive - microbiology</topic><topic>Major Article</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Moraxella</topic><topic>Moraxella (Branhamella) catarrhalis - classification</topic><topic>Moraxella (Branhamella) catarrhalis - genetics</topic><topic>Moraxella (Branhamella) catarrhalis - pathogenicity</topic><topic>Moraxella catarrhalis</topic><topic>Neisseriaceae Infections - genetics</topic><topic>Neisseriaceae Infections - microbiology</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Pharynx</topic><topic>Polymerase chain reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Respiratory Tract Infections - microbiology</topic><topic>RNA, Ribosomal, 16S - chemistry</topic><topic>Sputum</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bootsma, Hester J.</creatorcontrib><creatorcontrib>van der Heide, Han G. J.</creatorcontrib><creatorcontrib>van de Pas, Simone</creatorcontrib><creatorcontrib>Schouls, Leo M.</creatorcontrib><creatorcontrib>Mooi, Frits R.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bootsma, Hester J.</au><au>van der Heide, Han G. J.</au><au>van de Pas, Simone</au><au>Schouls, Leo M.</au><au>Mooi, Frits R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of Moraxella catarrhalis by DNA Typing: Evidence for a Distinct Subpopulation Associated with Virulence Traits</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>The Journal of Infectious Diseases</addtitle><date>2000-04-01</date><risdate>2000</risdate><volume>181</volume><issue>4</issue><spage>1376</spage><epage>1387</epage><pages>1376-1387</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Two DNA typing methods, probe-generated restriction fragment length polymorphism analysis and single-adapter amplified fragment length polymorphism analysis, were used to study the genetic relationships among 90 Moraxella catarrhalis strains. Both methods were found to be highly concordant, generating a dendrogram with 2 main branches. The division of the M. catarrhalis population into 2 subspecies was supported by analysis of the 16S rRNA sequences. Both β-lactamase—positive and β-lactamase—negative strains were found in all main branches, suggesting horizontal transfer of the β-lactamase gene. In contrast, 2 virulence traits, complement resistance and adherence to epithelial cells, were strongly associated with 1 of the 2 subspecies. The branch depth suggested that complement-resistant adherent strains diverged from a common ancestor more recently than did complement-sensitive nonadherent strains. These findings suggest the existence of subpopulations of M. catarrhalis that differ in virulence, and they may have implications for vaccine development.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>10762569</pmid><doi>10.1086/315374</doi><tpages>12</tpages></addata></record> |
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| subjects | Adult Antigens, Bacterial - genetics Antigens, Surface - genetics Bacterial Outer Membrane Proteins - genetics Bacteriology Base Sequence Biological and medical sciences Carrier State Child DNA DNA fingerprinting Epithelial cells Fundamental and applied biological sciences. Psychology Gels Humans Laryngitis - microbiology Lung Diseases, Obstructive - genetics Lung Diseases, Obstructive - microbiology Major Article Microbiology Molecular Sequence Data Moraxella Moraxella (Branhamella) catarrhalis - classification Moraxella (Branhamella) catarrhalis - genetics Moraxella (Branhamella) catarrhalis - pathogenicity Moraxella catarrhalis Neisseriaceae Infections - genetics Neisseriaceae Infections - microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Pharynx Polymerase chain reaction Polymorphism, Restriction Fragment Length Respiratory Tract Infections - microbiology RNA, Ribosomal, 16S - chemistry Sputum Virulence |
| title | Analysis of Moraxella catarrhalis by DNA Typing: Evidence for a Distinct Subpopulation Associated with Virulence Traits |
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