Comparison of porcine reproductive and respiratory syndrome virus growth in media supplemented with fetal bovine serum or a serum replacement
Department of Veterinary Microbiology and Preventive Medicine, Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames 50011, USA. Commercially available serum replacements are often used in cell culture as a cheaper and less variable substitute for fetal b...
Gespeichert in:
| Veröffentlicht in: | Journal of veterinary diagnostic investigation 2001-05, Vol.13 (3), p.276-279 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 279 |
|---|---|
| container_issue | 3 |
| container_start_page | 276 |
| container_title | Journal of veterinary diagnostic investigation |
| container_volume | 13 |
| creator | Schommer, SK Carpenter, SL Paul, PS |
| description | Department of Veterinary Microbiology and Preventive Medicine, Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
Commercially available serum replacements are often used in cell culture as a cheaper and less variable substitute for fetal bovine serum (FBS). The growth of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in CRL11171 cells maintained in a medium supplemented with FBS was compared with virus propagation in the same cell line maintained in the same medium with a serum replacement. The PRRSV replicated significantly better when the cell culture medium was supplemented with FBS. The results of this study have implications for the use of serum replacement-supplemented medium for PRRSV diagnosis by virus isolation. |
| doi_str_mv | 10.1177/104063870101300320 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71053268</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_104063870101300320</sage_id><sourcerecordid>49337468</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-ff20e86ef0bc3ae2e0a3156605ecbbbac9be12e6f60d7ea67492841fc8b0c13d3</originalsourceid><addsrcrecordid>eNqFkc-O0zAQxiMEYpeFF-CAfIFbWY-d2OkRVcsfaSUucLYmzrh1lcTBTlr1IXhn3DbSHpDg5JH9-77xfFMUb4F_BND6HnjJlaw1Bw6Scyn4s-IW1qVclWupnuc6A6szcVO8SmnPeSUqDS-LG4CyFgrkbfF7E_oRo09hYMGxMUTrB2KRxhja2U7-QAyHNl-k0UecQjyxdBraGHpiBx_nxLYxHKcd8wPrqfXI0jyOHfU0TNSyo89PjibsWBMOZ-tEce5ZiAyXMvfq0F4Er4sXDrtEb5bzrvj5-eHH5uvq8fuXb5tPjytb6mpaOSc41Yocb6xEEsRRQqUUr8g2TYN23RAIUk7xVhMqXa5FXYKzdcMtyFbeFR-uvnnKXzOlyfQ-Weo6HCjMyWjglRSq_i-Yg5a6vIDiCtoYUorkzBh9j_FkgJvztszf28qid4v73OTsniTLejLwfgEwWexcxMH69MSVoKS6GN1fuYRbMvswxyHH9-_Wy2A7v90dfSSTeuy6_BFh9ofWgzTSCK3kH-SAuvg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>49337468</pqid></control><display><type>article</type><title>Comparison of porcine reproductive and respiratory syndrome virus growth in media supplemented with fetal bovine serum or a serum replacement</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>SAGE Complete</source><source>Alma/SFX Local Collection</source><creator>Schommer, SK ; Carpenter, SL ; Paul, PS</creator><creatorcontrib>Schommer, SK ; Carpenter, SL ; Paul, PS</creatorcontrib><description>Department of Veterinary Microbiology and Preventive Medicine, Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
Commercially available serum replacements are often used in cell culture as a cheaper and less variable substitute for fetal bovine serum (FBS). The growth of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in CRL11171 cells maintained in a medium supplemented with FBS was compared with virus propagation in the same cell line maintained in the same medium with a serum replacement. The PRRSV replicated significantly better when the cell culture medium was supplemented with FBS. The results of this study have implications for the use of serum replacement-supplemented medium for PRRSV diagnosis by virus isolation.</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/104063870101300320</identifier><identifier>PMID: 11482613</identifier><language>eng</language><publisher>Los Angeles, CA: J Vet Diagn Invest</publisher><subject>Alphaarterivirus ; Animals ; Biological and medical sciences ; blood serum ; Cattle ; cell culture ; Cell Culture Techniques ; Cell Culture Techniques - methods ; Culture Media ; dietary supplements ; Fetal Blood ; fetus ; Fundamental and applied biological sciences. Psychology ; isolation & purification ; methods ; Microbiology ; pathogenicity ; physiology ; porcine reproductive and respiratory syndrome ; Porcine respiratory and reproductive syndrome virus ; Porcine respiratory and reproductive syndrome virus - isolation & purification ; Porcine respiratory and reproductive syndrome virus - pathogenicity ; Porcine respiratory and reproductive syndrome virus - physiology ; Specimen Handling ; virus replication</subject><ispartof>Journal of veterinary diagnostic investigation, 2001-05, Vol.13 (3), p.276-279</ispartof><rights>2001 American Association of Veterinary Laboratory Diagnosticians</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-ff20e86ef0bc3ae2e0a3156605ecbbbac9be12e6f60d7ea67492841fc8b0c13d3</citedby><cites>FETCH-LOGICAL-c475t-ff20e86ef0bc3ae2e0a3156605ecbbbac9be12e6f60d7ea67492841fc8b0c13d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/104063870101300320$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/104063870101300320$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14163620$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11482613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schommer, SK</creatorcontrib><creatorcontrib>Carpenter, SL</creatorcontrib><creatorcontrib>Paul, PS</creatorcontrib><title>Comparison of porcine reproductive and respiratory syndrome virus growth in media supplemented with fetal bovine serum or a serum replacement</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>Department of Veterinary Microbiology and Preventive Medicine, Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
Commercially available serum replacements are often used in cell culture as a cheaper and less variable substitute for fetal bovine serum (FBS). The growth of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in CRL11171 cells maintained in a medium supplemented with FBS was compared with virus propagation in the same cell line maintained in the same medium with a serum replacement. The PRRSV replicated significantly better when the cell culture medium was supplemented with FBS. The results of this study have implications for the use of serum replacement-supplemented medium for PRRSV diagnosis by virus isolation.</description><subject>Alphaarterivirus</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>blood serum</subject><subject>Cattle</subject><subject>cell culture</subject><subject>Cell Culture Techniques</subject><subject>Cell Culture Techniques - methods</subject><subject>Culture Media</subject><subject>dietary supplements</subject><subject>Fetal Blood</subject><subject>fetus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>isolation & purification</subject><subject>methods</subject><subject>Microbiology</subject><subject>pathogenicity</subject><subject>physiology</subject><subject>porcine reproductive and respiratory syndrome</subject><subject>Porcine respiratory and reproductive syndrome virus</subject><subject>Porcine respiratory and reproductive syndrome virus - isolation & purification</subject><subject>Porcine respiratory and reproductive syndrome virus - pathogenicity</subject><subject>Porcine respiratory and reproductive syndrome virus - physiology</subject><subject>Specimen Handling</subject><subject>virus replication</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-O0zAQxiMEYpeFF-CAfIFbWY-d2OkRVcsfaSUucLYmzrh1lcTBTlr1IXhn3DbSHpDg5JH9-77xfFMUb4F_BND6HnjJlaw1Bw6Scyn4s-IW1qVclWupnuc6A6szcVO8SmnPeSUqDS-LG4CyFgrkbfF7E_oRo09hYMGxMUTrB2KRxhja2U7-QAyHNl-k0UecQjyxdBraGHpiBx_nxLYxHKcd8wPrqfXI0jyOHfU0TNSyo89PjibsWBMOZ-tEce5ZiAyXMvfq0F4Er4sXDrtEb5bzrvj5-eHH5uvq8fuXb5tPjytb6mpaOSc41Yocb6xEEsRRQqUUr8g2TYN23RAIUk7xVhMqXa5FXYKzdcMtyFbeFR-uvnnKXzOlyfQ-Weo6HCjMyWjglRSq_i-Yg5a6vIDiCtoYUorkzBh9j_FkgJvztszf28qid4v73OTsniTLejLwfgEwWexcxMH69MSVoKS6GN1fuYRbMvswxyHH9-_Wy2A7v90dfSSTeuy6_BFh9ofWgzTSCK3kH-SAuvg</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>Schommer, SK</creator><creator>Carpenter, SL</creator><creator>Paul, PS</creator><general>J Vet Diagn Invest</general><general>SAGE Publications</general><general>American Association of Veterinary Laboratory Diagnosticians</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>7X8</scope></search><sort><creationdate>20010501</creationdate><title>Comparison of porcine reproductive and respiratory syndrome virus growth in media supplemented with fetal bovine serum or a serum replacement</title><author>Schommer, SK ; Carpenter, SL ; Paul, PS</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-ff20e86ef0bc3ae2e0a3156605ecbbbac9be12e6f60d7ea67492841fc8b0c13d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Alphaarterivirus</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>blood serum</topic><topic>Cattle</topic><topic>cell culture</topic><topic>Cell Culture Techniques</topic><topic>Cell Culture Techniques - methods</topic><topic>Culture Media</topic><topic>dietary supplements</topic><topic>Fetal Blood</topic><topic>fetus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>isolation & purification</topic><topic>methods</topic><topic>Microbiology</topic><topic>pathogenicity</topic><topic>physiology</topic><topic>porcine reproductive and respiratory syndrome</topic><topic>Porcine respiratory and reproductive syndrome virus</topic><topic>Porcine respiratory and reproductive syndrome virus - isolation & purification</topic><topic>Porcine respiratory and reproductive syndrome virus - pathogenicity</topic><topic>Porcine respiratory and reproductive syndrome virus - physiology</topic><topic>Specimen Handling</topic><topic>virus replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schommer, SK</creatorcontrib><creatorcontrib>Carpenter, SL</creatorcontrib><creatorcontrib>Paul, PS</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schommer, SK</au><au>Carpenter, SL</au><au>Paul, PS</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of porcine reproductive and respiratory syndrome virus growth in media supplemented with fetal bovine serum or a serum replacement</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>13</volume><issue>3</issue><spage>276</spage><epage>279</epage><pages>276-279</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>Department of Veterinary Microbiology and Preventive Medicine, Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
Commercially available serum replacements are often used in cell culture as a cheaper and less variable substitute for fetal bovine serum (FBS). The growth of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in CRL11171 cells maintained in a medium supplemented with FBS was compared with virus propagation in the same cell line maintained in the same medium with a serum replacement. The PRRSV replicated significantly better when the cell culture medium was supplemented with FBS. The results of this study have implications for the use of serum replacement-supplemented medium for PRRSV diagnosis by virus isolation.</abstract><cop>Los Angeles, CA</cop><pub>J Vet Diagn Invest</pub><pmid>11482613</pmid><doi>10.1177/104063870101300320</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1040-6387 |
| ispartof | Journal of veterinary diagnostic investigation, 2001-05, Vol.13 (3), p.276-279 |
| issn | 1040-6387 1943-4936 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71053268 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SAGE Complete; Alma/SFX Local Collection |
| subjects | Alphaarterivirus Animals Biological and medical sciences blood serum Cattle cell culture Cell Culture Techniques Cell Culture Techniques - methods Culture Media dietary supplements Fetal Blood fetus Fundamental and applied biological sciences. Psychology isolation & purification methods Microbiology pathogenicity physiology porcine reproductive and respiratory syndrome Porcine respiratory and reproductive syndrome virus Porcine respiratory and reproductive syndrome virus - isolation & purification Porcine respiratory and reproductive syndrome virus - pathogenicity Porcine respiratory and reproductive syndrome virus - physiology Specimen Handling virus replication |
| title | Comparison of porcine reproductive and respiratory syndrome virus growth in media supplemented with fetal bovine serum or a serum replacement |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T09%3A19%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20porcine%20reproductive%20and%20respiratory%20syndrome%20virus%20growth%20in%20media%20supplemented%20with%20fetal%20bovine%20serum%20or%20a%20serum%20replacement&rft.jtitle=Journal%20of%20veterinary%20diagnostic%20investigation&rft.au=Schommer,%20SK&rft.date=2001-05-01&rft.volume=13&rft.issue=3&rft.spage=276&rft.epage=279&rft.pages=276-279&rft.issn=1040-6387&rft.eissn=1943-4936&rft_id=info:doi/10.1177/104063870101300320&rft_dat=%3Cproquest_cross%3E49337468%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=49337468&rft_id=info:pmid/11482613&rft_sage_id=10.1177_104063870101300320&rfr_iscdi=true |