Role of cAMP-Dependent Protein Kinase during Growth and Early Development of Dictyostelium discoideum
cAMP-dependent protein kinase (PKA) is an essential regulator of gene expression and cell differentiation during multicellular development of Dictyostelium discoideum. Here we show that PKA activity also regulates gene expression during the growth phase and at the transition from growth to developme...
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| Veröffentlicht in: | Developmental biology 2000-05, Vol.221 (1), p.101-111 |
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| creator | Primpke, Gerd Iassonidou, Vasiliki Nellen, Wolfgang Wetterauer, Birgit |
| description | cAMP-dependent protein kinase (PKA) is an essential regulator of gene expression and cell differentiation during multicellular development of Dictyostelium discoideum. Here we show that PKA activity also regulates gene expression during the growth phase and at the transition from growth to development. Overexpression of PKA leads to overexpression of the discoidinIγ promoter, while expression of the discoidinIγ promoter is reduced when PKA activity is reduced, either by expression of a dominant negative mutant of the regulatory subunit or by disruption of the gene for the catalytic subunit (PKA-C). The discoidin phenotype of PKA-C null cells is cell autonomous. In particular, normal secretion of discoidin-inducing factors was demonstrated. In addition, PKA-C null cells are able to respond to media conditioned by PSF and CMF. We conclude that PKA is a major activator of discoidin expression. However, it is not required for production or transduction of the inducing extracellular signals. Therefore, PKA-dependent and PKA-independent pathways regulate the expression of the discoidin genes. |
| doi_str_mv | 10.1006/dbio.2000.9662 |
| format | Article |
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Here we show that PKA activity also regulates gene expression during the growth phase and at the transition from growth to development. Overexpression of PKA leads to overexpression of the discoidinIγ promoter, while expression of the discoidinIγ promoter is reduced when PKA activity is reduced, either by expression of a dominant negative mutant of the regulatory subunit or by disruption of the gene for the catalytic subunit (PKA-C). The discoidin phenotype of PKA-C null cells is cell autonomous. In particular, normal secretion of discoidin-inducing factors was demonstrated. In addition, PKA-C null cells are able to respond to media conditioned by PSF and CMF. We conclude that PKA is a major activator of discoidin expression. However, it is not required for production or transduction of the inducing extracellular signals. Therefore, PKA-dependent and PKA-independent pathways regulate the expression of the discoidin genes.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1006/dbio.2000.9662</identifier><identifier>PMID: 10772794</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Differentiation ; CMF ; Culture Media, Conditioned ; Cyclic AMP-Dependent Protein Kinases - genetics ; Cyclic AMP-Dependent Protein Kinases - metabolism ; development ; Dictyostelium ; Dictyostelium - enzymology ; Dictyostelium - growth & development ; Dictyostelium discoideum ; discoidin ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Enzymologic ; Genes, Reporter ; growth ; Phenotype ; PKA ; Promoter Regions, Genetic ; PSF</subject><ispartof>Developmental biology, 2000-05, Vol.221 (1), p.101-111</ispartof><rights>2000 Academic Press</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-8c6b2da97a8f2f3445f30f71c3ad572719a6a03addb27df8fb38f92ba941683d3</citedby><cites>FETCH-LOGICAL-c411t-8c6b2da97a8f2f3445f30f71c3ad572719a6a03addb27df8fb38f92ba941683d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0012160600996623$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10772794$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Primpke, Gerd</creatorcontrib><creatorcontrib>Iassonidou, Vasiliki</creatorcontrib><creatorcontrib>Nellen, Wolfgang</creatorcontrib><creatorcontrib>Wetterauer, Birgit</creatorcontrib><title>Role of cAMP-Dependent Protein Kinase during Growth and Early Development of Dictyostelium discoideum</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>cAMP-dependent protein kinase (PKA) is an essential regulator of gene expression and cell differentiation during multicellular development of Dictyostelium discoideum. Here we show that PKA activity also regulates gene expression during the growth phase and at the transition from growth to development. Overexpression of PKA leads to overexpression of the discoidinIγ promoter, while expression of the discoidinIγ promoter is reduced when PKA activity is reduced, either by expression of a dominant negative mutant of the regulatory subunit or by disruption of the gene for the catalytic subunit (PKA-C). The discoidin phenotype of PKA-C null cells is cell autonomous. In particular, normal secretion of discoidin-inducing factors was demonstrated. In addition, PKA-C null cells are able to respond to media conditioned by PSF and CMF. We conclude that PKA is a major activator of discoidin expression. However, it is not required for production or transduction of the inducing extracellular signals. Therefore, PKA-dependent and PKA-independent pathways regulate the expression of the discoidin genes.</description><subject>Animals</subject><subject>Cell Differentiation</subject><subject>CMF</subject><subject>Culture Media, Conditioned</subject><subject>Cyclic AMP-Dependent Protein Kinases - genetics</subject><subject>Cyclic AMP-Dependent Protein Kinases - metabolism</subject><subject>development</subject><subject>Dictyostelium</subject><subject>Dictyostelium - enzymology</subject><subject>Dictyostelium - growth & development</subject><subject>Dictyostelium discoideum</subject><subject>discoidin</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Genes, Reporter</subject><subject>growth</subject><subject>Phenotype</subject><subject>PKA</subject><subject>Promoter Regions, Genetic</subject><subject>PSF</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFL5DAYxYMo6-ju1aPk5K1jkrZJehRHXVFRZBf2FtLki0baZkxaZf77TRkPXsTTxwe_93i8h9ARJUtKCD-1rQ9LRghZNpyzHbSgpKmLmlf_dtGCEMoKygnfRwcpvWSqlLL8gfYpEYKJplogeAwd4OCwObt7KFawhsHCMOKHGEbwA77xg06A7RT98ISvYngfn7EeLL7QsdvgFbxBF9b9LMkmK2_GTUgjdH7qsfXJBG9h6n-iPae7BL8-7iH6e3nx5_x3cXt_dX1-dluYitKxkIa3zOpGaOmYK6uqdiVxgppS2zoHpo3mmuTHtkxYJ11bStewVjcV5bK05SE62fquY3idII2qzxmg6_QAYUpKUFIzzsW3IBWC1pKTDC63oIkhpQhOraPvddwoStS8gJoXUPMCal4gC44_nKe2B_sJ31aeAbkFIBfx5iGqZDwMBqyPYEZlg__K-z9Xc5W0</recordid><startdate>20000501</startdate><enddate>20000501</enddate><creator>Primpke, Gerd</creator><creator>Iassonidou, Vasiliki</creator><creator>Nellen, Wolfgang</creator><creator>Wetterauer, Birgit</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20000501</creationdate><title>Role of cAMP-Dependent Protein Kinase during Growth and Early Development of Dictyostelium discoideum</title><author>Primpke, Gerd ; Iassonidou, Vasiliki ; Nellen, Wolfgang ; Wetterauer, Birgit</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-8c6b2da97a8f2f3445f30f71c3ad572719a6a03addb27df8fb38f92ba941683d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Cell Differentiation</topic><topic>CMF</topic><topic>Culture Media, Conditioned</topic><topic>Cyclic AMP-Dependent Protein Kinases - genetics</topic><topic>Cyclic AMP-Dependent Protein Kinases - metabolism</topic><topic>development</topic><topic>Dictyostelium</topic><topic>Dictyostelium - enzymology</topic><topic>Dictyostelium - growth & development</topic><topic>Dictyostelium discoideum</topic><topic>discoidin</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Genes, Reporter</topic><topic>growth</topic><topic>Phenotype</topic><topic>PKA</topic><topic>Promoter Regions, Genetic</topic><topic>PSF</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Primpke, Gerd</creatorcontrib><creatorcontrib>Iassonidou, Vasiliki</creatorcontrib><creatorcontrib>Nellen, Wolfgang</creatorcontrib><creatorcontrib>Wetterauer, Birgit</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Primpke, Gerd</au><au>Iassonidou, Vasiliki</au><au>Nellen, Wolfgang</au><au>Wetterauer, Birgit</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of cAMP-Dependent Protein Kinase during Growth and Early Development of Dictyostelium discoideum</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>2000-05-01</date><risdate>2000</risdate><volume>221</volume><issue>1</issue><spage>101</spage><epage>111</epage><pages>101-111</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>cAMP-dependent protein kinase (PKA) is an essential regulator of gene expression and cell differentiation during multicellular development of Dictyostelium discoideum. Here we show that PKA activity also regulates gene expression during the growth phase and at the transition from growth to development. Overexpression of PKA leads to overexpression of the discoidinIγ promoter, while expression of the discoidinIγ promoter is reduced when PKA activity is reduced, either by expression of a dominant negative mutant of the regulatory subunit or by disruption of the gene for the catalytic subunit (PKA-C). The discoidin phenotype of PKA-C null cells is cell autonomous. In particular, normal secretion of discoidin-inducing factors was demonstrated. In addition, PKA-C null cells are able to respond to media conditioned by PSF and CMF. We conclude that PKA is a major activator of discoidin expression. However, it is not required for production or transduction of the inducing extracellular signals. Therefore, PKA-dependent and PKA-independent pathways regulate the expression of the discoidin genes.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10772794</pmid><doi>10.1006/dbio.2000.9662</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Cell Differentiation CMF Culture Media, Conditioned Cyclic AMP-Dependent Protein Kinases - genetics Cyclic AMP-Dependent Protein Kinases - metabolism development Dictyostelium Dictyostelium - enzymology Dictyostelium - growth & development Dictyostelium discoideum discoidin Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Genes, Reporter growth Phenotype PKA Promoter Regions, Genetic PSF |
| title | Role of cAMP-Dependent Protein Kinase during Growth and Early Development of Dictyostelium discoideum |
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