Guinea Pig Fertilin Exhibits Restricted Lateral Mobility in Epididymal Sperm and Becomes Freely Diffusing during Capacitation
The guinea pig sperm protein fertilin functions in sperm–egg plasma membrane binding. Fertilin is initially present in the plasma membrane of the whole head in testicular sperm, then becomes concentrated into the posterior head domain during epididymal passage. Fertilin remains localized to the post...
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| Veröffentlicht in: | Developmental biology 2001-08, Vol.236 (2), p.502-509 |
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| creator | Cowan, Ann E. Koppel, Dennis E. Vargas, Louis A. Hunnicutt, Gary R. |
| description | The guinea pig sperm protein fertilin functions in sperm–egg plasma membrane binding. Fertilin is initially present in the plasma membrane of the whole head in testicular sperm, then becomes concentrated into the posterior head domain during epididymal passage. Fertilin remains localized to the posterior head plasma membrane following the acrosome reaction, when it functions in sperm–egg interaction. Fluorescence redistribution after photobleaching was used to examine the lateral mobility of fertilin in both acrosome-intact and acrosome-reacted sperm. Fertilin exhibited highly restricted lateral mobility in both testicular and epididymal sperm (D < 10−10 cm2/s). However, fertilin in acrosome-reacted sperm was highly mobile within the membrane bilayer (D = 1.8 × 10−9 cm2/s and %R = 84). Measurement of the lateral mobility of fertilin in capacitated, acrosome-intact sperm revealed two populations of cells. In approximately one-half of the cells, lateral mobility of fertilin was similar to sperm freshly isolated from the cauda epididymis; while in the other half fertilin was highly mobile. The release of fertilin from interactions that restrict its lateral mobility may regulate its function in sperm–egg interaction. |
| doi_str_mv | 10.1006/dbio.2001.0343 |
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Fertilin is initially present in the plasma membrane of the whole head in testicular sperm, then becomes concentrated into the posterior head domain during epididymal passage. Fertilin remains localized to the posterior head plasma membrane following the acrosome reaction, when it functions in sperm–egg interaction. Fluorescence redistribution after photobleaching was used to examine the lateral mobility of fertilin in both acrosome-intact and acrosome-reacted sperm. Fertilin exhibited highly restricted lateral mobility in both testicular and epididymal sperm (D < 10−10 cm2/s). However, fertilin in acrosome-reacted sperm was highly mobile within the membrane bilayer (D = 1.8 × 10−9 cm2/s and %R = 84). Measurement of the lateral mobility of fertilin in capacitated, acrosome-intact sperm revealed two populations of cells. In approximately one-half of the cells, lateral mobility of fertilin was similar to sperm freshly isolated from the cauda epididymis; while in the other half fertilin was highly mobile. The release of fertilin from interactions that restrict its lateral mobility may regulate its function in sperm–egg interaction.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1006/dbio.2001.0343</identifier><identifier>PMID: 11476588</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acrosome Reaction - drug effects ; ADAM Proteins ; Animals ; Calcium - metabolism ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Diffusion - drug effects ; Epididymis - cytology ; Epididymis - metabolism ; Fertilins ; Fluorescence ; Guinea Pigs ; Ionophores - pharmacology ; Male ; Membrane Glycoproteins - metabolism ; Metalloendopeptidases - metabolism ; Protein Transport - drug effects ; Sperm Capacitation - drug effects ; Spermatozoa - cytology ; Spermatozoa - drug effects ; Spermatozoa - metabolism</subject><ispartof>Developmental biology, 2001-08, Vol.236 (2), p.502-509</ispartof><rights>2001 Academic Press</rights><rights>Copyright 2001 Academic Press.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-8fc199c9fe4a13b500e92a800309d80a07e515e17d60a39c1afa5b1cdc5bca923</citedby><cites>FETCH-LOGICAL-c380t-8fc199c9fe4a13b500e92a800309d80a07e515e17d60a39c1afa5b1cdc5bca923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/dbio.2001.0343$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11476588$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cowan, Ann E.</creatorcontrib><creatorcontrib>Koppel, Dennis E.</creatorcontrib><creatorcontrib>Vargas, Louis A.</creatorcontrib><creatorcontrib>Hunnicutt, Gary R.</creatorcontrib><title>Guinea Pig Fertilin Exhibits Restricted Lateral Mobility in Epididymal Sperm and Becomes Freely Diffusing during Capacitation</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>The guinea pig sperm protein fertilin functions in sperm–egg plasma membrane binding. Fertilin is initially present in the plasma membrane of the whole head in testicular sperm, then becomes concentrated into the posterior head domain during epididymal passage. Fertilin remains localized to the posterior head plasma membrane following the acrosome reaction, when it functions in sperm–egg interaction. Fluorescence redistribution after photobleaching was used to examine the lateral mobility of fertilin in both acrosome-intact and acrosome-reacted sperm. Fertilin exhibited highly restricted lateral mobility in both testicular and epididymal sperm (D < 10−10 cm2/s). However, fertilin in acrosome-reacted sperm was highly mobile within the membrane bilayer (D = 1.8 × 10−9 cm2/s and %R = 84). Measurement of the lateral mobility of fertilin in capacitated, acrosome-intact sperm revealed two populations of cells. In approximately one-half of the cells, lateral mobility of fertilin was similar to sperm freshly isolated from the cauda epididymis; while in the other half fertilin was highly mobile. The release of fertilin from interactions that restrict its lateral mobility may regulate its function in sperm–egg interaction.</description><subject>Acrosome Reaction - drug effects</subject><subject>ADAM Proteins</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Diffusion - drug effects</subject><subject>Epididymis - cytology</subject><subject>Epididymis - metabolism</subject><subject>Fertilins</subject><subject>Fluorescence</subject><subject>Guinea Pigs</subject><subject>Ionophores - pharmacology</subject><subject>Male</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Metalloendopeptidases - metabolism</subject><subject>Protein Transport - drug effects</subject><subject>Sperm Capacitation - drug effects</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - metabolism</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEuLFDEUhYMoTju6dSlZuav2pqpTlSy1Z3oUWhQf4C6kklvjlXqZpMRe-N9N0Q2uXF04fPfA-Rh7LmArAOpXvqVpWwKILVS76gHbCNCykPXu20O2yXFZiBrqK_Ykxh8AUClVPWZXQuyaWiq1YX_uFhrR8o90zw8YEvU08tvf36mlFPknjCmQS-j50SYMtufvpzYz6cRXbiZP_jTk-POMYeB29PwNumnAyA8BsT_xG-q6JdJ4z_0S1rO3s3WUbKJpfMoedbaP-Oxyr9nXw-2X_dvi-OHu3f71sXCVglSozgmtne5wZ0XVSgDUpVV5DmivwEKDUkgUja_BVtoJ21nZCuedbJ3VZXXNXp575zD9XPIoM1B02Pd2xGmJphEgy6rRGdyeQRemGAN2Zg402HAyAswq3KzCzSrcrMLzw4tL89IO6P_hF8MZUGcA875fhMFERzg69BTQJeMn-l_3X0yrkSM</recordid><startdate>20010815</startdate><enddate>20010815</enddate><creator>Cowan, Ann E.</creator><creator>Koppel, Dennis E.</creator><creator>Vargas, Louis A.</creator><creator>Hunnicutt, Gary R.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010815</creationdate><title>Guinea Pig Fertilin Exhibits Restricted Lateral Mobility in Epididymal Sperm and Becomes Freely Diffusing during Capacitation</title><author>Cowan, Ann E. ; Koppel, Dennis E. ; Vargas, Louis A. ; Hunnicutt, Gary R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-8fc199c9fe4a13b500e92a800309d80a07e515e17d60a39c1afa5b1cdc5bca923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Acrosome Reaction - drug effects</topic><topic>ADAM Proteins</topic><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Diffusion - drug effects</topic><topic>Epididymis - cytology</topic><topic>Epididymis - metabolism</topic><topic>Fertilins</topic><topic>Fluorescence</topic><topic>Guinea Pigs</topic><topic>Ionophores - pharmacology</topic><topic>Male</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Metalloendopeptidases - metabolism</topic><topic>Protein Transport - drug effects</topic><topic>Sperm Capacitation - drug effects</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cowan, Ann E.</creatorcontrib><creatorcontrib>Koppel, Dennis E.</creatorcontrib><creatorcontrib>Vargas, Louis A.</creatorcontrib><creatorcontrib>Hunnicutt, Gary R.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cowan, Ann E.</au><au>Koppel, Dennis E.</au><au>Vargas, Louis A.</au><au>Hunnicutt, Gary R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Guinea Pig Fertilin Exhibits Restricted Lateral Mobility in Epididymal Sperm and Becomes Freely Diffusing during Capacitation</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>2001-08-15</date><risdate>2001</risdate><volume>236</volume><issue>2</issue><spage>502</spage><epage>509</epage><pages>502-509</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>The guinea pig sperm protein fertilin functions in sperm–egg plasma membrane binding. Fertilin is initially present in the plasma membrane of the whole head in testicular sperm, then becomes concentrated into the posterior head domain during epididymal passage. Fertilin remains localized to the posterior head plasma membrane following the acrosome reaction, when it functions in sperm–egg interaction. Fluorescence redistribution after photobleaching was used to examine the lateral mobility of fertilin in both acrosome-intact and acrosome-reacted sperm. Fertilin exhibited highly restricted lateral mobility in both testicular and epididymal sperm (D < 10−10 cm2/s). However, fertilin in acrosome-reacted sperm was highly mobile within the membrane bilayer (D = 1.8 × 10−9 cm2/s and %R = 84). Measurement of the lateral mobility of fertilin in capacitated, acrosome-intact sperm revealed two populations of cells. In approximately one-half of the cells, lateral mobility of fertilin was similar to sperm freshly isolated from the cauda epididymis; while in the other half fertilin was highly mobile. The release of fertilin from interactions that restrict its lateral mobility may regulate its function in sperm–egg interaction.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11476588</pmid><doi>10.1006/dbio.2001.0343</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acrosome Reaction - drug effects ADAM Proteins Animals Calcium - metabolism Cell Membrane - drug effects Cell Membrane - metabolism Diffusion - drug effects Epididymis - cytology Epididymis - metabolism Fertilins Fluorescence Guinea Pigs Ionophores - pharmacology Male Membrane Glycoproteins - metabolism Metalloendopeptidases - metabolism Protein Transport - drug effects Sperm Capacitation - drug effects Spermatozoa - cytology Spermatozoa - drug effects Spermatozoa - metabolism |
| title | Guinea Pig Fertilin Exhibits Restricted Lateral Mobility in Epididymal Sperm and Becomes Freely Diffusing during Capacitation |
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